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General Information
D. melanogaster
FlyBase ID
Feature type
Computed Breakpoints include


Sequence coordinates
3L:8,745,326..8,745,326 (Df(3L)ED4421:bk1)
3L:9,384,075..9,384,075 (Df(3L)ED4421:bk2)
Member of large scale dataset(s)

A set of isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Initial core set of 209 isogenic deletions provides ~60% euchromatic genome coverage.

Nature of Aberration
Cytological Order
Class of aberration (relative to wild type)
Class of aberration (relative to progenitor)
Causes alleles
Carries alleles
Transposon Insertions
Formalized genetic data
Genetic mapping information

This deletion removes a single ribosomal protein-coding gene (though other genes are also removed).

Comments on Cytology

Confirmed by 3-step PCR confirmation as shown in Confirmed by lack of complementation to a known lethal mutation predicted to fall between the progenitor insertions.

Limits of break 1 computationally determined from location of progenitor P insertion on genome sequence between P{PZ}SrpRβrK561&P{lacW}l(3)j5B6j5B6 and P{PZ}l(3)0162901629&P{PZ}mRpL1210534 Limits of break 2 computationally determined from location of progenitor P insertion on genome sequence between P{EP}Hsp26EP3336&P{EP}Hsp26EP3315 and P{PZ}fry02240

Sequence Crossreferences
DNA sequence
Protein sequence
Gene Deletion and Duplication Data
Genes Deleted / Disrupted
Complementation Data
Completely deleted / disrupted
Partially deleted / disrupted
Molecular Data
Completely deleted
Partially deleted
Genes NOT Deleted / Disrupted
Complementation Data
Molecular Data
Genes Duplicated
Complementation Data
Completely duplicated
Partially duplicated
Molecular Data
Completely duplicated
Partially duplicated
Genes NOT Duplicated
Complementation Data
Molecular Data
Affected Genes Inferred by Location
Phenotypic Data
In combination with other aberrations

Df(3L)knittrigΔ1 embryos transheterozygous with Df(3L)ED4421 show reduced formation of ganglionic tracheal branches with significant penetrance - the majority of embryos show disruption in at least one ganglionic branch. No abnormal phenotype is detected for midline glial cells.

Df(3L)knittrigΔ1 flies transheterozygous with Df(3L)ED4421 show reduced viability and only rare escaper flies are found. Most mutants die during pupal stages or as pharate adults. Most escapers are unable to undergo complete wing inflation, causing wrinkled wings. Some escapers show partially inflated and abnormally opaque wings. Mutant pupal wings appar normal.

NOT in combination with other aberrations

Df(3L)ED4421 results in cardiac enlargement in adults, but no significant reduction in fractional shortening, as compared to controls.

Flies heterozygous for the deletion do not show a Minute bristle phenotype.

Stocks (2)
Notes on Origin
Balancer / Genotype Variants of the Aberration
Separable Components
Other Comments
Synonyms and Secondary IDs (2)
References (18)