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General Information
Symbol
Df(3L)ED4543
Species
D. melanogaster
Name
FlyBase ID
FBab0035791
Feature type
Computed Breakpoints include

[70C6-70C6];[70F4-70F4];

Sequence coordinates
3L:13,935,225..13,935,225 (Df(3L)ED4543:bk1)
3L:14,758,040..14,758,040 (Df(3L)ED4543:bk2)
Member of large scale dataset(s)
Dfs_DrosDel_set1

A set of isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Initial core set of 209 isogenic deletions provides ~60% euchromatic genome coverage.

Nature of Aberration
Cytological Order
Class of aberration (relative to wild type)
Class of aberration (relative to progenitor)
Breakpoints
Causes alleles
Carries alleles
Transposon Insertions
Formalized genetic data
Genetic mapping information
Comments
Comments on Cytology

Confirmed by 3-step PCR confirmation as shown in http://www.drosdel.org.uk/del_confirm.php Confirmed by lack of complementation to a known lethal mutation predicted to fall between the progenitor insertions.

Limits of break 1 computationally determined from location of progenitor P insertion on genome sequence between P{lacW}l(3)L5212L5212&P{PZ}l(3)0587105871 and P{EP}EP3561EP3561 Limits of break 2 computationally determined from location of progenitor P insertion on genome sequence between P{PZ}Mpcp00564 and P{PZ}l(3)rO220rO220

Sequence Crossreferences
DNA sequence
Protein sequence
Gene Deletion and Duplication Data
Genes Deleted / Disrupted
Complementation Data
Completely deleted / disrupted
Partially deleted / disrupted
Molecular Data
Completely deleted
Partially deleted
Genes NOT Deleted / Disrupted
Complementation Data
 
Molecular Data
 
Genes Duplicated
Complementation Data
Completely duplicated
Partially duplicated
Molecular Data
Completely duplicated
Partially duplicated
Genes NOT Duplicated
Complementation Data
 
Molecular Data
 
Affected Genes Inferred by Location
Phenotypic Data
In combination with other aberrations
NOT in combination with other aberrations

Newly formed primordial germ cells (PGCs) of wild-type stage 4 and 5 embryos (syncytial blastoderm and cellular blastoderm, respectively) are predominantly spherical. By contrast, similarly staged embryos from mothers heterozygous for Df(3L)ED4543 display a dominant phenotype in which most embryos (60-80%) include multiple PGCs with strikingly abnormal morphology. The cells have an irregular shape, often with small protrusions. In some cases, the protrusions appear to pinch off from the larger part of the cell. Often, the mutant PGCs are not as tightly coalesced as for wild-type. Instead of the one or two layers of closely packed PGCs, gaps sometimes appear between the PGCs, and the layered organization could be disrupted.

Stocks (2)
Notes on Origin
Discoverer
 
Balancer / Genotype Variants of the Aberration
 
Separable Components
 
Other Comments
 
Synonyms and Secondary IDs (4)
References (22)