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General Information
Symbol
Df(3L)BSC395
Species
D. melanogaster
Name
FlyBase ID
FBab0045206
Feature type
Computed Breakpoints include

[68F1-68F1];[68F2-68F2];

Sequence coordinates
3L:12,004,542..12,004,542 (Df(3L)BSC395:bk1)
3L:12,081,822..12,081,822 (Df(3L)BSC395:bk2)
Member of large scale dataset(s)
Dfs_BSC_set2

A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.

Nature of Aberration
Cytological Order
Class of aberration (relative to wild type)
Class of aberration (relative to progenitor)
Breakpoints
Causes alleles
Carries alleles
Formalized genetic data
Genetic mapping information
Comments

Breakpoint from FlyBase's release 5 sequence location of progenitor insertion.

Comments on Cytology

Exelixis, Inc. determined the insertion site of the progenitor P{XP}d03723 to be at Release 3 genomic coordinate 12039353 on chromosome arm 3L. The Gene Disruption project determined the insertion site of the progenitor P{XP}d03723 to be at Release 3 genomic coordinate 12039369 on arm 3L. This corresponds to 68F2 on both the Release 3 and 5 genome maps. The predicted position of the progenitor PBac{RB}CG6928e01931 on the Release 5 map is 68F1. Consequently, the cytological breakpoints of Df(3L)BSC395 are predicted to be 68F1;68F2.

Sequence Crossreferences
DNA sequence
Protein sequence
Gene Deletion and Duplication Data
Genes Deleted / Disrupted
Complementation Data
Completely deleted / disrupted
Partially deleted / disrupted
Molecular Data
Completely deleted
Partially deleted
Genes NOT Deleted / Disrupted
Complementation Data
 
Molecular Data
 
Genes Duplicated
Complementation Data
Completely duplicated
Partially duplicated
Molecular Data
Completely duplicated
Partially duplicated
Genes NOT Duplicated
Complementation Data
 
Molecular Data
 
Affected Genes Inferred by Location
Phenotypic Data
In combination with other aberrations

Inferred to overlap with: Df(3L)BSC840.

Inferred to overlap with: Df(3L)BSC574.

NOT in combination with other aberrations

Df(3L)BSC395 homozygous embryos show a regular muscle pattern but the segmental border muscles are reduced to mini muscles and some unfused myoblasts are visible, as compared to controls.

Stocks (1)
Notes on Origin
Discoverer
 
Balancer / Genotype Variants of the Aberration
 
Separable Components
 
Other Comments
 

The presence of P+PBac{XP5.RB3}BSC395 was verified using the PCR methods and primers described in FBrf0175003, with the substitution of the primer 5'-CCAATGCGTTTATTTCAGGTCACG-3' for the RB3' plus or RB3' minus primer in the Hybrid PCR protocol in the Supplementary Methods.

Synonyms and Secondary IDs (2)
References (9)