Mutant flies show normal responses to both low salt (50mM NaCl) and high salt (500mM NaCl) in a two-way choice test.

Df(3L)Gr64^ab flies prefer fructose to sucrose, whereas wild-type flies prefer sucrose. In addition Df(3L)Gr64^ab flies fail to show a bias in favor of the 5mM over 1mM sucrose, but retain the ability to select the 5mM over 2mM fructose. Thus, the behavioral response to sucrose, but not fructose, is defective in Df(3L)Gr64^ab flies. Df(3L)Gr64^ab flies exhibit a normal preference for 20mM arabinose over 2mM fructose. However, unlike wild-type, the Df(3L)Gr64^ab mutant prefers fructose over either glucose or maltose. Also in contrast to wildtype, Df(3L)Gr64^ab selects fructose rather than trehalose, which is surprising given that trehalose is the ligand that activates Tre. Df(3L)Gr64^ab flies show a normal aversion to quinine and caffeine.

The frequencies of action potentials induced by sucrose, glucose, maltose, and trehalose are dramatically reduced in Df(3L)Gr64^ab flies.