[61C8-61C8];[61D2-61D2];
A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
61C8;61D2
Breakpoint based on release 3 sequence coordinate from Thibault et al., 2004, Supplementary Table 2 (FBrf0174227) or 3 (FBrf0174228), converted to release 5 coordinate.
Breakpoint from FlyBase's release 5 sequence location of progenitor insertion.
Inferred to overlap with: Df(3L)BSC632.
Inferred to overlap with: Df(3L)ED202.
The presence of P+PBac{XP5.WH5}BSC440 was verified using the PCR methods and primers described in FBrf0175003.
Exelixis, Inc. determined the insertion site of P{XP}d06287 to be Release 3 genomic coordinate 688843 on chromosome arm 3L and the insertion site of PBac{WH}f03465 to be Release 3 genomic coordinate 807399 on arm 3L. The Gene Disruption project determined the insertion site of P{XP}d06287 to be Release 3 genomic coordinate 688835 on arm 3L. The P{XP}d06287 insertion site corresponds to 61C8 and the PBac{WH}f03465 insertion site corresponds to 61D2 on the Release 3 and Release 5 genome maps. Consequently, the cytological breakpoints of Df(3L)BSC440 are predicted to be 61C8;61D2.