A set of ~800 largely isogenic deficiency stocks created by FLP-induced recombination between FRT-carrying transgenic insertions; molecularly defined deletion endpoints correspond to initial location of the progenitor insertions. Designed to fill gaps in deletion coverage and breakpoint placement; also used to replace older available deficiencies that have not been molecularly mapped.
Df(3R)BSC789/Df(3R)BSC806 first instar larvae do not show any dorsal closure defects, although head development is abnormal.
Inferred to overlap with: Df(3R)BSC874.
Homozygotes die as embryos. 26% of the embryos produce cuticles with strong head involution and dorsal closure defects, including kinked features and holes in the dorsal epidermis. The ventro-lateral epidermis and the denticle belts are unaffected. Some cell-cell contacts seem to be lost in the amnioserosa of homozygous stage 13 embryos, while no gaps are seen in the ectoderm or leading edge.
The presence of P+PBac{XP5.RB3}BSC789 was verified using the PCR methods and primers described in FBrf0175003 with the substitution of the primer 5’-GCTTCTAAACGCTTACGCATAAACGATG-3’ for the RB3’ plus or RB3’ minus primer in the Hybrid PCR protocol in the Supplementary Methods.
The breakpoints of Df(3R)BSC789 predicted from the Release 5 genomic coordinates of the progenitor PBac{RB}e00251 and P{XP}Pkc98Ed08513 transposable element insertion sites are 3R:24645856 ;24866229 and the cytological breakpoints predicted from these coordinates are 98E5;98F6.