Open Close
General Information
Symbol
Dmel\amn1
Species
D. melanogaster
Name
FlyBase ID
FBal0000500
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

The open reading frame of the mutant is identical to wild type. The amn mRNA is strongly reduced in the mutant compared to wild type.

FlyBase curator comment: FBrf0081985 suggests that the amn1 mutation is due to a single base pair mutation which results in a frameshift. However, FBrf0102808 shows that the open reading frame in the amn1 mutant is identical to wild type, and that the level of mRNA produced by the mutant is reduced compared to wild-type levels.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 1 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

As in wild type, amn1 mutant males exhibit inhibition of the female contact-induced male-male aggression seen in wild type males that have previously been exposed to females.

Compared with wild-type, amn1 show a decrease in axonal branch number without affecting synaptic varicosity number or innervation length.

amn1 mutant larvae show a significant delay in response to noxious heat compared to control flies. These flies also exhibit a delay in their jump response to noxious heat of 9 seconds.

amn1 mutants make choices according to their recent experience and completely ignore the past experience in a 60-60V 1hr delay choice, similar to wild-type. No significant difference is observed between the 1hr Anesthesia-resistant memory (ARM) of a single conditioning event and that followed by a second conditioning event in amn1 mutants.

The choice PI in the 60-30 V 1hr delay protocol is not significantly different from the 30 V immediate memory PI in amn1 mutants, compared to wild-type where it is significantly lower.

amn1 and amnX8/amn1 flies are able to learn to associate an odor with a sugar reward (although they have a small but significant initial performance defect), but they forget this association within 60 minutes of training.

Muscles of third instar amn1 larvae show a reduction in the L-type Ca2+ current compared to control larvae.

Mutant animals, unlike wild-type, do not show significant age-related memory impairment, 1 hour memory in aged flies was not significantly different from young mutant flies.

In amn1 homozygotes no effect is seen on the amplitude of the synchronous oscillation of intracellular calcium concentration seen in wild type Kenyon cells.

Shows defects in memory retention both immediately (initial learning) or 180 minutes (3 hour memory) after training.

Hemizygous males and homozygous females show increased sensitivity to ethanol in an inebriometer assay.

After presentation of electric shock with a first odour or with fresh air, amn1 flies show a strongly reduced avoidance of a second, different odour compared to wild-type flies.

amn1 flies kept in constant darkness have a smaller lamina volume than amn1 flies kept in constant light, as is also seen for wild-type flies.

In experiments involving 'operant' conditioning, with heat as the aversive unconditioned stimulus, amn1 exhibits a small decrement in learning per se and subsequently has no detectable memory.

It appears that short-term memory is defective in the mutant (in shock-odor tests), with long-term memory being normal.

Groups of amn1 flies exhibit apparently abnormal acquisition of learning in tests using visual cues.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement
Reference
Phenotype Manifest In
Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

amn1, amnX8 and amn28A all fail to complement each other for memory retention either immediately (initial learning) or 180 minutes (3 hour memory) after training.

Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
Comments
Comments

Memory decay of rad1 flies is faster than in amn1 flies. Cold-induced anaesthesia treatment has little effect on memory.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
References (31)