Internal 25kb partial duplication from position 46 to 71 and a complex insertion of more than 40kb including two roo elements. Truncated roo element has inserted at position 71 within intron sequences in opposite transcriptional orientation. The 5' half of a roo element has inserted at position 46 in opposite transcriptional orientation. The duplication gives the gene three promoters, P1, P2A and P2B. Dominant mutant phenotype due to ectopic expression from promoter Antp P2A.
25kb duplication of the exon 3 region with a roo transposon separating the duplicated sequences.
The AntpNs/+ antenna-to-leg transformation phenotype is exacerbated by Abd-BFab7-12 (which deletes the Fab-7 element) after a AntpNs Abd-BFab7-12 stock has been maintained for several generations; no difference in the AntpNs phenotype is seen immediately after the establishment of the AntpNs Abd-BFab7-12 stock, however, an exacerbation of the AntpNs phenotype emerges in subsequent generations of the stock, reaching a plateau after 3 to 5 generations.
The mutant arista phenotype seen in AntpNs/+ adults is partially suppressed if they are also heterozygous for Gug519 (36% of flies have normal arista development), Gug1757 (26% of flies have normal arista development) or Gug3333 (28% of flies have normal arista development), but is unaffected if they are also heterozygous for Gug2295.
ash2 alleles affect the expressivity or penetrance of the antenna to leg transformation. ash21, ash29, ash211, ash212 and ash218 enhance the transformation phenotype, ash22 and ash24 suppress the transformation phenotype.