FlyBase Allele Report: Dmel\Dll[17]

General Information

Symbol

Dmel\Dll¹⁷

Species

D. melanogaster

Name

FlyBase ID

FBal0001014

Feature type

allele

Associated gene

Dmel\Dll

Associated Insertion(s)

Carried in Construct

Also Known As

Dll^SA1

Key Links

Allele class

amorphic allele - genetic evidence

Mutagen

X ray

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Cohen et al., 1993, Cohen and Jurgens, 1989)

Mutagen

X ray

(Cohen and Jurgens, 1989)

Progenitor genotype

Cytology

Description

5.5kb deletion removing the exon encoding the amino terminal two thirds of the homeodomain.

(Cohen et al., 1993)

about 6 kb deletion around about 120 kb; removes middle exon; Coordinates estimated from Cohen, Broenner, Kuettner, Jurgens and Jaeckle (1989); origin undefined; positive values extend to left.

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal chemosensory behavior | dominant | embryonic stage

(Plavicki et al., 2012)

abnormal chemosensory behavior | larval stage (with Dll³)

(Plavicki et al., 2012)

abnormal neuroanatomy | embryonic stage (with Dll³)

(Plavicki et al., 2012)

abnormal neuroanatomy | larval stage (with Dll³)

(Plavicki et al., 2012)

decreased occurrence of cell division | larval stage | somatic clone

(Gorfinkiel et al., 1997)

lethal | recessive

(Gorfinkiel et al., 1999)

lethal - all die before end of P-stage | recessive

(Dong et al., 2000)

some die during pharate adult stage | recessive

(Dong et al., 2000)

visible (with Dll¹⁵)

(Galindo et al., 2011)

visible | homeotic | recessive

(Dong et al., 2000)

visible | recessive

(Campbell and Tomlinson, 1998)

visible | recessive | somatic clone

(Gorfinkiel et al., 1999)

visible | somatic clone

(Moreno and Morata, 1999, Wu and Cohen, 1999, Gorfinkiel et al., 1997)

Phenotype Manifest In

adult cuticle | somatic clone

(Moreno and Morata, 1999)

antenna

(Dong et al., 2000)

arista

(Dong et al., 2000)

arista | somatic clone

(Gorfinkiel et al., 1997)

cercus | male | somatic clone

(Gorfinkiel et al., 1999)

cercus | somatic clone

(Moreno and Morata, 1999)

distiproboscis | somatic clone

(Yasunaga et al., 2006)

dorsal organ | embryonic stage

(Plavicki et al., 2012)

dorsal organ | larval stage (with Dll³)

(Plavicki et al., 2012)

dorsal row of double row of wing sensilla | somatic clone | somatic clone

(Gorfinkiel et al., 1997)

dorsal row of triple row of wing sensilla | somatic clone

(Gorfinkiel et al., 1997)

epiproct | female | somatic clone

(Gorfinkiel et al., 1999)

femur

(Dong et al., 2000)

femur (with Dll¹⁵)

(Galindo et al., 2011)

femur | somatic clone

(Gorfinkiel et al., 1997)

leg

(Dong et al., 2000)

leg | somatic clone

(Campbell and Tomlinson, 1998, Gorfinkiel et al., 1997)

leg disc | somatic clone

(Wu and Cohen, 1999, Gorfinkiel et al., 1997)

macrochaeta & leg | somatic clone

(Gorfinkiel et al., 1997)

medial row of triple row of wing sensilla | somatic clone

(Gorfinkiel et al., 1997)

mesothoracic femoral bract | somatic clone

(Gorfinkiel et al., 1997)

mesothoracic leg disc | larval stage

(Gorfinkiel et al., 1997)

mesothoracic tibial bract | somatic clone

(Gorfinkiel et al., 1997)

metathoracic femoral bract | somatic clone

(Gorfinkiel et al., 1997)

metathoracic leg disc | larval stage

(Gorfinkiel et al., 1997)

metathoracic tibial bract | somatic clone

(Gorfinkiel et al., 1997)

prothoracic femoral bract | somatic clone

(Gorfinkiel et al., 1997)

prothoracic leg disc | larval stage

(Gorfinkiel et al., 1997)

prothoracic tibial bract | somatic clone

(Gorfinkiel et al., 1997)

second segment of antenna | somatic clone

(Gorfinkiel et al., 1997)

tarsal segment (with Dll¹⁵)

(Galindo et al., 2011)

tarsal segment | somatic clone

(Wu and Cohen, 1999, Gorfinkiel et al., 1997)

terminal organ | embryonic stage

(Plavicki et al., 2012)

third segment of antenna

(Dong et al., 2000)

third segment of antenna | somatic clone

(Gorfinkiel et al., 1997)

tibia

(Dong et al., 2000)

tibia (with Dll¹⁵)

(Galindo et al., 2011)

tibia | proximal | somatic clone

(Gorfinkiel et al., 1997)

tibia | somatic clone

(Gorfinkiel et al., 1997)

trochanter | somatic clone

(Gorfinkiel et al., 1997)

ventral row of double row of wing sensilla | somatic clone

(Gorfinkiel et al., 1997)

ventral row of triple row of wing sensilla

(Gorfinkiel et al., 1997)

wing & macrochaeta | somatic clone

(Gorfinkiel et al., 1997)

wing blade anterior compartment | somatic clone

(Gorfinkiel et al., 1997)

wing blade posterior compartment | somatic clone

(Gorfinkiel et al., 1997)

wing margin | somatic clone

(Gorfinkiel et al., 1997)

wing margin bristle | cell autonomous | somatic clone

(Campbell and Tomlinson, 1998)

wing margin bristle | somatic clone

(Gorfinkiel et al., 1997)

wing vein | cell non-autonomous | somatic clone

(Gorfinkiel et al., 1997)

wing vein | somatic clone

(Gorfinkiel et al., 1997)

Detailed Description

Statement

Reference

Dll¹⁷/Dll³ mutant larvae show a significantly impaired behavioural response to an attractive odorant (EtOAc). Some larvae show dorsal organ malformations but there appears to be no correlation between the larvae with cuticular defects and those with olfactory impairment. Motor activity appears normal, and allowing more time for the larva to respond (10 minutes rather than 5 minutes) does not change the response indices.

Heterozygous Dll¹⁷ mutant larvae show a significantly impaired response to an attractive odorant (EtOAc). No malformations are seen in in the cuticular components of the dorsal organ.

Heterozygous Dll¹⁷ mutant embryos have fewer neurons and fewer support cells in the dorsal organ (DO) and terminal organ than wild type animals. No cell death is seen (TUNEL staining) and the remaining neurons exhibit aberrant morphologies. The DO dendrites are loosely associated rather than tightly bundled.

(Plavicki et al., 2012)

Large minute clones of Dll¹⁷ result in almost all portions of distiproboscis being eliminated. The mediproboscis is not eliminated by these clones.

(Yasunaga et al., 2006)

The basal capsule of the arista is almost normal in heterozygous flies.

(Emerald et al., 2003)

Dll³/Dll¹⁷ animals die as pharate adults. They show truncations of the antenna, such as deletion of antennal segment 3 and the arista. A complete loss of the tarsal segments and shortening of both the femur and tibia is seen in the legs.

(Dong et al., 2000)

Dll¹⁷ somatic clones do not develop anal plates in males, or dorsal anal plates in females. Clones do not show any detectable alterations in the male external genitalia. Clones do not affect the development of the female ventral anal plates.

(Gorfinkiel et al., 1999)

Dll¹⁷ somatic clones produce areas of defective cuticle and are unable to form anal plates.

(Moreno and Morata, 1999)

The distribution of homozygous clones along the proximodistal axis of the leg differs from the distribution of control clones; the ratio of distal-to-proximal clones is 2:1 for control clones and 1:4 for homozygous Dll¹⁷ clones, suggesting that homozygous Dll¹⁷ clones are lost distally. Homozygous clones in the tarsal segments segregate out of the surrounding wild-type imaginal disc epithelium. Vesicles of homozygous Dll¹⁷ tissue are sometimes seen inside the tarsal segments of the legs of adult flies.

(Wu and Cohen, 1999)

Homozygous clones can be recovered in the dorsal femur when they are generated at any stage in development (from embryo to mid-third instar larva), although early in development their frequency is reduced compared to wild-type clones. When the leg is composed almost entirely of Dll¹⁷ tissue then the region more distal to the coxa is represented by only a small stump of tissue. The characteristic hairs and bristles found at the wing margin are deleted in homozygous clones at the margin. The effect of these clones is autonomous.

(Campbell and Tomlinson, 1998)

Homozygous clones induced in the leg discs do not proliferate. Homozygous clones induced in the leg during the first and second larval instar stages are only found in the pleural and coxa regions and produce no morphological abnormalities. Homozygous clones induced in the leg during the third larval instar stage are frequently seen in the distal regions of the leg. The majority of clones in the trochanter and tibia-tarsus region form vesicles that invaginate inside the appendage. These clones often contain bristles and trichomes that do not resemble those in the vicinity of the clone. Clones in the femur and proximal tibia contain bristles without bracts. Homozygous clones induced in the antenna during the first larval instar stage are able to differentiate the aI antennal segment and a small part of the aII antennal segment, but are unable to form the rest of the aII segment, segment aIII or the arista. Homozygous clones induced in the antenna during the third larval instar stage tend to form vesicles that are separated from the surrounding tissue in segments aII and aIII. Clones in the arista often differentiate bracted bristles, suggesting an antennal to leg transformation. Homozygous clones induced in the wing during the larval stage affect the wing margin; the triple row of bristles in the anterior compartment and the double row of long hairs in the posterior compartment are eliminated. Clones away from the margin often affect vein differentiation in the vicinity of the margin. This affect can be nonautonomous as wild-type cells in the vicinity of the clone are often affected. The clones develop socketed bristles in the posterior compartment and also develop a halo of pigment.

(Gorfinkiel et al., 1997)

Dll null mutant embryos produced both leg and imaginal discs following in vivo culture.

(Cohen et al., 1993)

Embryos have normal mouth hooks and ventral organs, but missing an average of 5 cirri from the anterior row and 7 cirri from the posterior row. Also the ventral organ often includes extra papillae of unknown origin.

(O'Hara et al., 1993)

Wnt5 expression in head and thoracic segments is absent in embryos presumed to be homozygous for this allele.

(Eisenberg et al., 1992)

Greatly reduced femur and tibia, associated with normal trochanter and coxa.

(Cohen et al., 1989)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Dll¹⁷ is a suppressor | partially of homeotic | somatic clone phenotype of Scer\GAL4^Tub.PU, btd^UAS.cSa

(Estella and Mann, 2010)

Other

Statement

Reference

Df(3R)ex56, Dll¹⁷ has visible phenotype

(Emerald et al., 2003)

Dll¹⁷, danr^ex35 has visible phenotype

(Emerald et al., 2003)

Phenotype Manifest In

Suppressor of

Statement

Reference

Dll¹⁷ is a suppressor | partially of wing | somatic clone phenotype of Scer\GAL4^Tub.PU, btd^UAS.cSa

(Estella and Mann, 2010)

Dll¹⁷ is a suppressor of leg | distal | ectopic | somatic clone phenotype of Scer\GAL4^Tub.PU, btd^UAS.cSa

(Estella and Mann, 2010)

NOT Suppressor of

Statement

Reference

Dll¹⁷ is a non-suppressor of leg | proximal | ectopic | somatic clone phenotype of Scer\GAL4^Tub.PU, btd^UAS.cSa

(Estella and Mann, 2010)

Other

Statement

Reference

Df(3R)ex56, Dll¹⁷ has basal cylinder phenotype

(Emerald et al., 2003)

Df(3R)ex56, Dll¹⁷ has tarsal segment | ectopic phenotype

(Emerald et al., 2003)

Dll¹⁷, danr^ex35 has basal cylinder phenotype

(Emerald et al., 2003)

Dll¹⁷, danr^ex35 has tarsal segment | ectopic phenotype

(Emerald et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

A Dll¹⁷ mutant background partially suppresses the ectopic leg tissue phenotype seen when btd^Scer\UAS.cSa clones are generated under the control of Scer\GAL4^tub.PU using the MARCM system. These clones cannot generate distal leg fates, but are able to produce what appears to be proximal leg tissue.

(Estella and Mann, 2010)

Dll¹⁷ danr^ex35 or Dll¹⁷ Df(3R)ex56 double heterozygotes show ectopic tarsal bristles in the basal capsule of the arista.

(Emerald et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Df(2R)ED4065/Dll¹⁷ is partially rescued by Dll^+m312

(Galindo et al., 2011)

Comments

Some Dll¹⁷/Df(2R)ED4065 animals carrying Dll^+m312 survive until the pharate adult stage. These animals show severe truncation and malformation of the medial and distal leg segments. The distal tibia and tarsal regions are absent, the femur is shortened and the trochanter is deformed. The bristles on the femur lack bracts, in contrast to wild type. Mechanosensory bristles of the wing margin are missing.

(Galindo et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

78356

w^*; P{neoFRT}42D Dll¹⁷/CyO

Notes on Origin

Discoverer

Comments

Clonal analysis indicates that the requirement for Dll in the femur and most of the tibia is lost by about the early third larval instar stage.

(Campbell and Tomlinson, 1998)

Strong Dll allele.

(Cohen et al., 1989)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

Ba¹⁷

(Lindsley and Zimm, 1992)

Ba^SA1

(Cohen et al., 1993, Lindsley and Zimm, 1992)

Df(2R)SA1

Dll¹⁷

Dll^sa1

(Giorgianni and Mann, 2011)

DllSA1

(Dong et al., 2002)

Dll[SA1]

(Enriquez et al., 2018)

dll^SA-1

(Bertet et al., 2014)

dll^SA1

(Plavicki et al., 2012)

Name Synonyms

Secondary FlyBase IDs

FBab0002035

References (43)

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