FlyBase Allele Report: Dmel\car[1]

General Information

Symbol

Dmel\car¹

Species

D. melanogaster

Name

FlyBase ID

FBal0001541

Feature type

allele

Associated gene

Dmel\car

Associated Insertion(s)

Carried in Construct

Also Known As

carnation¹

Key Links

Genomic Maps

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Allele class

Mutagen

X ray

Nature of the Allele

Allele class

Mutagen

X ray

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

point_mutation

X:19,566,417..19,566,417 [+]

Nucleotide change:

C19566417G

Amino acid change:

L26V | car-PA; L26V | car-PB; L26V | car-PC

Reported amino acid change:

L26V

Comment:

One of two amino acid substitutions observed in car¹ mutant. Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Sevrioukov et al., 1999)

point_mutation

X:19,567,087..19,567,087 [+]

Nucleotide change:

G19567087T

Amino acid change:

G249V | car-PA; G249V | car-PB; G249V | car-PC

Reported amino acid change:

G249V

Comment:

One of two amino acid substitutions observed in car¹ mutant. Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Sevrioukov et al., 1999)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Cytology

Nature of the lesion

Statement

Reference

Mutated residue Gly249 is a conserved residue in car homologues of different species.

(Sevrioukov et al., 1999)

Amino acid replacement: L26V.

(Sevrioukov et al., 1999)

Amino acid replacement: G249V.

(Sevrioukov et al., 1999)

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates neurofibromatosis

modeled by Nf1^E2

(Walker et al., 2013)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal eye color

(Sevrioukov et al., 1999)

decreased cell death

(Yacobi-Sharon et al., 2013)

short lived | male

(Simonsen et al., 2007)

viable

(Warner et al., 1998)

visible | recessive

Phenotype Manifest In

Detailed Description

Statement

Reference

car¹ homozygotes or heterozygotes do not exhibit a significant increase in the proportion of hyperplastic testes, as compared to controls.

(Napoletano et al., 2017)

car¹ mutant males exhibit ~40% less spermatogonial cyst death compared to controls.

(Yacobi-Sharon et al., 2013)

car¹ mutants exhibit an endosome clustering phenotype.

(Gailite et al., 2012)

car¹ mutants exhibit retinal degeneration after 9-days of constant light.

(Kinser and Dolph, 2012)

Both number and morphology of LysoTracker-positive puncta and lipid droplets during ecdysone-induced autophagy in car¹ male wandering third instar larvae is comparable to wild-type.

(Bartlett et al., 2011)

Mutant flies show some decrease in ommochrome pigment levels and a significant reduction in drosopterin pigment levels compared to wild type.

(Harris et al., 2011)

Mutant males have a shortened adult life span compared to controls, with a mean life span of 17.42 days at 25^o.

(Simonsen et al., 2007)

Hemocyte endosomal degradation is significantly reduced in car¹ mutants compared to wild-type cells. This reduction also correlates with an increased severity of eye-colour defect. car¹ mutants show a small but significant impairment in endosomal degradation. There is no difference between in the morphology and formation of the multi-vesicular body and the small dense organelle between car¹ and wild-type cells. Cells from car¹ animals exhibit a block in transition of small dense vesicles to tubular-vesicular compartments. Endosomes in car¹ mutant cells exhibit wild-type fusion characteristics and mature into fusion-inaccessible small dense structures.

(Sriram et al., 2003)

Brain histology abnormal.

(McCarthy and Nickla, 1980)

Malpighian tubule colour: pale yellow; hard to distinguish from wild type before third instar.

(Beadle, 1937)

Eye color autonomous in transplant into wild-type host.

(Beadle and Ephrussi, 1936)

Eye colour: dark ruby. Body shape and proportions seem rounded.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^arm.PS, car¹, rush^UAS.GFP has partially lethal - majority live phenotype, suppressible by sta^+t4.4/Df(1)rush⁴

(Gailite et al., 2012)

Enhancer of

Statement

Reference

car[+]/car¹ is an enhancer of visible phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, bchs^EP2299

(Simonsen et al., 2007)

Suppressor of

Statement

Reference

car¹ is a suppressor of decreased body size | pupal stage phenotype of Nf1^E2

(Walker et al., 2013)

Other

Statement

Reference

Scer\GAL4^arm.PS, car¹, rush^UAS.GFP has partially lethal - majority live phenotype

(Gailite et al., 2012)

car¹, dor¹ has lethal | prepupal stage phenotype

(Sriram et al., 2003)

car¹, lt¹ has lethal | recessive phenotype

(Warner et al., 1998, Nickla, 1977)

car¹, lt¹/lt¹¹ has lethal phenotype

(Warner et al., 1998)

car¹, lt¹/lt¹¹ has lethal | recessive phenotype

(Warner et al., 1998)

car¹, lt^unspecified has lethal phenotype

(Nickla, 1977)

car¹, dor¹ has lethal | pupal stage phenotype

(Nash, 1971)

Phenotype Manifest In

Suppressed by

Statement

Reference

car¹ has eye phenotype, suppressible by Cys^GMR.PK

(Kinser and Dolph, 2012)

car¹ has ommatidium phenotype, suppressible by Cys^GMR.PK

(Kinser and Dolph, 2012)

car¹ has rhabdomere phenotype, suppressible by Cys^GMR.PK

(Kinser and Dolph, 2012)

NOT suppressed by

Statement

Reference

car¹ has phenotype, non-suppressible by su(Hw)²

(Lindsley and Grell, 1968)

Enhancer of

Statement

Reference

car[+]/car¹ is an enhancer of eye phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, bchs^EP2299

(Simonsen et al., 2007)

Suppressor of

Statement

Reference

car¹/car¹ is a suppressor of phenotype of l(2)gd1^d7

(Schneider et al., 2013)

car¹ is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, Sk2^UAS.cYa

(Yonamine et al., 2011)

car¹ is a suppressor of photoreceptor cell phenotype of Scer\GAL4^GMR.PF, Sk2^UAS.cYa

(Yonamine et al., 2011)

car¹ is a suppressor of ommatidium phenotype of Scer\GAL4^GMR.PF, Sk2^UAS.cYa

(Yonamine et al., 2011)

car¹ is a suppressor of rhabdomere phenotype of Scer\GAL4^GMR.PF, Sk2^UAS.cYa

(Yonamine et al., 2011)

Other

Statement

Reference

Dp(1;Y)1E, car¹ has late endosome phenotype

(Sriram et al., 2003)

car¹, lt¹ has embryonic/larval brain phenotype

(McCarthy and Nickla, 1980)

car¹, lt¹ has ventral nerve cord phenotype

(Nickla et al., 1980)

Additional Comments

Genetic Interactions

Statement

Reference

car¹/car¹, p53^E8/+ adults do not exhibit a significant increase in the proportion of hyperplastic testes, as compared to car¹/car¹ adults, p53^E8/+ adults, or wild-type controls.

(Napoletano et al., 2017)

Homozygosity of car¹ suppresses the ectopic N activation phenotype (ectopic expression of wg) in l(2)gd1^d7 wing discs.

(Schneider et al., 2013)

Expression of rush^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^arm.PS decreases the fraction of car¹ flies eclosing. This effect is rescued by Df(1)rush⁴, sta^+t4.4.

(Gailite et al., 2012)

The presence of Cys^GMR rescues the retinal degeneration seen in car¹ after 9-days of constant light.

(Kinser and Dolph, 2012)

The reduction in drosopterin pigment levels that is seen in car¹ flies is not affected by HPS4^W515X.

The reduction in drosopterin pigment levels seen in flies carrying w^dsRNA.GMR is not enhanced by car¹.

(Harris et al., 2011)

A car¹ mutant background suppresses the retinal degeneration seen upon expression of Sk2^Scer\UAS.cYa under the control of Scer\GAL4^GMR.PF in the developing eye through enhanced lysosomal degradation.

(Yonamine et al., 2011)

Eye colour: car¹; p^p eyes are lighter than either single mutant. The levels of red pigment in the double mutants is comparable to that in car¹ single mutant eyes and the level of brown pigment is comparable to p^p single mutant eyes.

(Falcon-Perez et al., 2007)

The double mutant, car¹ dor¹ does not survive beyond the prepupal stage. In cell culture, car¹ dor¹ cells exhibit the most severe defect in endosomal degradation, compared to either single mutant. There is a small but significant difference between the degradation defect in dor¹ car¹ and dor¹, consistent with a role for both car and dor in endosomal degradation. Endosomes in cells from car¹ dor¹ mutants, although incapable of a morphological transition to small dense vesicles, are capable of fusion at early times and normal maturation. The presence of Dp(1;Y)1E in car¹ mutants blocks the transition from large to small endosomes during maturation.

(Sriram et al., 2003)

Lethal focus of the lt car lethal interaction as measured in car¹/+; lt¹/lt¹, car¹/0; lt¹/lt¹ gynandromorphs is in ventral nervous tissue.

(Nickla et al., 1980)

Dies as larva when mother car¹; lt^unspecified/+ or car¹/+; lt^unspecified and as pupa when mother car¹/+; lt^unspecified/+.

(Nickla, 1977)

Eye colour: brownish yellow to brown with bw¹. Eye colour: yellow-brown with st¹.

(Mainx, 1938)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

car^Δ146

(Akbar et al., 2009)

car^Δ141

(Akbar et al., 2009)

car^Δ163

(Akbar et al., 2009)

car^Δ33

(Akbar et al., 2009)

Rescued by

car¹ is rescued by car^GMR.PS

(Sevrioukov et al., 1999)

Comments

Images (2)

Holtzman, S. and Kaufman, T. (2013)

Mutant

Wild-type

Stocks (45)

Bloomington

car¹

car¹ su(f)⁸

579

Df(1)JF5, f¹ car¹/FM7a

788

In(1)rst³, y¹ car¹

ec¹ ct⁶ sable¹ car¹/FM6

1321

In(1)sdx, sdx¹, y² f¹ car¹

Kyoto

105680

car¹

101065

car¹ su(f)⁸

101194

v¹ f^3N car¹

105990

Df(1)JF5, f¹ car¹/FM7a

105690

ec¹ ct⁶ sable¹ car¹/FM6

102034

In(1)sc⁷ + In(1)AM, car¹ / FM4, B⁺

More stocks available...

Notes on Origin

Discoverer

Comments

Reduced recovery in gynandromorphs with male parts car dor.

(Nash, 1971)

Lethal focus domineering; fate maps to ventral nervous system.

(Nickla et al., 1980)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

car¹

(Napoletano et al., 2017, Shimizu et al., 2017, Walker et al., 2013, Yacobi-Sharon et al., 2013, Gailite et al., 2012, Kinser and Dolph, 2012, Harris et al., 2011, Yonamine et al., 2011, Popodi, 2010.10.8, Popodi et al., 2010-, Akbar et al., 2009, Christensen and Cook, 2007.3.22, Falcon-Perez et al., 2007, Pulipparacharuvil et al., 2005)

carn¹

(Simonsen et al., 2007)

carnation¹

(Bartlett et al., 2011)

Name Synonyms

carnation1

(Simonsen et al., 2007)

Secondary FlyBase IDs

References (29)

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