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General Information
Symbol
Dmel\crc1
Species
D. melanogaster
Name
FlyBase ID
FBal0001818
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Mutagen
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

A21318382G

Amino acid change:

Q179R | crc-PA; Q96R | crc-PB; Q179R | crc-PE; Q170R | crc-PF

Reported amino acid change:

Q171R

Comment:

The annotated CDS is 8aa longer at the amino terminus than the published CDS accounting for the difference in the reported and annotated mutation locations.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: Q171R.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Up to 2% adult escapers are seen among crc1 hemizygotes. Mutants show several lethal phases during larval, pupal and adult development. Hemizygotes show 15-50% of their lethality after pupariation. Hemizygous puparia are generally aberrant to varying degrees (approximately 5% are indistinguishable from wild type). The defects include a failure to evert the anterior spiracles, and a retention of a larval shape, which is thinned, elongated and sometimes curved to one side. In the most severe cases, the abdominal gas bubble, which normally forms approximately 6 hours after pupariation, does not appear, although the larval mouthparts are later expelled. Head eversion fails or is incomplete and the leg and wing discs do not completely elongate. Segmentation and differentiation of the abdomen usually fail, although in some cases the anterior abdominal segments differentiate. Other aspects of adult development proceed normally, resulting in the appearance of mature eye pigments and darkened macrochaetae and differentiation of the wings and legs. Hemizygous females show markedly reduced fecundity. 5-10% of hemizygous adults fail to expand their wings and fully tan the adult cuticle. Other hemizygous adults show more subtle defects involving the wings, legs, scutellum, scutellar bristles, halteres and dorsal thorax.

Low rate of chitin deposition during prepupal chitin deposition period. Studies of pupal cuticles suggest that crc1 homozygotes are unable to evert their cephalic complexes at pupation. Total chitin content studies of the pupal cuticle cannot exclude the possibilty of specific increases in chitin content of the head and anterior thorax region.

Head eversion is totally or partially suppressed, wings and legs are shortened, abdomen is longer than normal. Homozygotes die within the puparium after completion of imaginal development.

Homozygotes frequently die as larvae; failure to eliminate chitinous mouthparts at the larval molt frequently results in larvae with supernumerary mouth parts; homozygotes undergo pupation but rarely eclose from puparia. Imaginal head is not everted from thorax. Except for slightly reduced eyes and shortened legs, wings and thoracic bristles, the head and thorax are fully differentiated; frequently fail to pass gas bubble to anterior retro-opercular position, thus failing to provide space for head eversion. Head eversion is inhibited by integument being more rigid than normal. Mutant integument contains more glucosamine than normal; Sparrow and Chadfield (1982) unable to confirm. Feeding glucosamine to wild-type larvae produces a phenocopy very similar to crc. Abdomen often shows no differentiation and internal organ development is arrested at pupal stage. Lethality suppressed by su(crc)1 (Sparrow, 1981). RK3.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement
Reference
NOT Enhancer of
Statement
Reference
Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference
Phenotype Manifest In
Suppressed by
Statement
Reference

crc1 has phenotype, suppressible by Su(crc)2Su2

crc1 has phenotype, suppressible by Su(crc)1

Enhancer of
Statement
Reference
NOT Suppressor of
Statement
Reference

crc1/crcR6 is a non-suppressor of eye disc phenotype of Scer\GAL4GMR.PF, ninaEG69D.UAS

Additional Comments
Genetic Interactions
Statement
Reference

The massive apoptosis seen in the larval eye discs of animals expressing ninaEG69D.Scer\UAS under the control of Scer\GAL4GMR.PF is not suppressed by crcR6/crc1.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (4)
Notes on Origin
Discoverer

Hadorn, 1942.

E. Hadorn.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Name Synonyms
lethal(2)cryptocephal
Secondary FlyBase IDs
    References (10)