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General Information
Symbol
Dmel\Dfd16
Species
D. melanogaster
Name
FlyBase ID
FBal0002389
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Dfdw21, DfdrW21
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

G6793812A

Reported nucleotide change:

G?A

Amino acid change:

W346term | Dfd-PA

Reported amino acid change:

W346term

Comment:

TGG to TGA

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology

Polytene chromosomes normal.

Nature of the lesion
Statement
Reference

Amino acid replacement: W346term.

Nucleotide substitution: G?A.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Dfd16 mutant clones encompassing the maxillary palp region have deleted palps and disordered suborbital vibrissae. Clones on the posterior of the head display altered postorbital and occipital bristles.

Mutant embryos show a fusion of the maxillary and mandibular segments.

Stage 12 Dfd16/Dfd12 embryos show displacement of the maxillary segment and mandibular segments due to an accumulation of supernumerary cells at the ventral side of both segments. Cells in the ventral part of the maxillary segment are elongated, instead of round like wild-type cells.

The mouthhooks are missing and the lateralgraten are shortened in mutant larvae.

In Dfd12/Dfd16 embryos, the maxillary cirri primordium and the anterior boundary of the dorsal ridge, between the mandibular and maxillary segments, are missing. The number of apoptotic cells in the maxillary segment is reduced when compared to wild-type.

Homozygous embryos lack cirri, mouth hooks, the ventral organ and the dental sclerite, the lateral process is truncated and the lateral bar of the H-piece is missing. A portion of the maxillary sense organ is missing and the remaining structure does not align properly with the antennal sense organ.

Mutants lack mouth hooks and cirri.

Dfd12/Df(3R)Scr embryos lack the mouthhooks and cirri, while the more posterior mouthparts are unaffected. The mouthhook and cirri defects are rescued in Dfd12/Df(3R)Scr embryos expressing DfdScer\UAS.cBa under the control of Scer\GAL469B. Head involution fails in these animals and the posterior mouthparts fail to form. Dfd12/Dfd16 embryos lack the mouthhooks and cirri, while the more posterior mouthparts are unaffected.

The morphology of the gnathal lobes is abnormal in Dfd16 embryos.

Homozygous embryos lack mouth hooks and cirri and the lateralgraten are shortened.

Dfd16 Scr4 double homozygous embryos exhibit failure of the labial lobes to migrate ventrally or internalise. Ectopic external sensory organs, derived from failure of the labial lobes to internalise, are the same as for Scr- embryos.

Double mutant combinations with cnc- embryos show additive effects in the head skeleton.

Dfd is required but not sufficient for 1.28 expression.

No salivary gland defects.

In Ubxhs.PG embryos that are homozygous for Dfd16 the cirri and mouthhooks are missing.

Failure head involution and head defects: defects in structures derived from the maxillary, mandibular and premandibular regions of the embryonic head. Mitotic recombination analysis suggests that in the adult Dfd affects the head structures homologous to those of the embryo.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Suppressed by
Enhancer of
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference

In Dfd16;Scr4 double mutant embryos the corpus allata and prothoracic glands do not form.

Addition of DfdScer\UAS.cBa to Dfd16;Scr4 double mutant embryos (under the control of Scer\GAL4salm-459.2) results in both the Mx and Lb segments forming corpora allata.

Addition of ScrScer\UAS.cMa to Dfd16;Scr4 double mutant embryos (under the control of Scer\GAL4salm-459.2) results in both the Mx and Lb segments forming prothoracic glands.

Addition of UbxScer\UAS.cCa to Dfd16;Scr4 double mutant embryos (under the control of Scer\GAL4salm-459.2) results in both the Mx and Lb segments forming tracheal tubes instead of migratory gland primordia.

Addition of Abd-BScer\UAS.cCa to Dfd16;Scr4 double mutant embryos (under the control of Scer\GAL4salm-459.2) results in both the Mx and Lb segments forming tracheal tubes instead of migratory gland primordia.

Addition of AntpScer\UAS.cMb to Dfd16;Scr4 double mutant embryos (under the control of Scer\GAL4salm-459.2) results in both the Mx and Lb segments forming tracheal tubes instead of migratory gland primordia. results in both the Mx and Lb segments forming prothoracic glands.

The formation of the maxillary-mandibular boundary is completely lost in gcmN7-4, Dfd16 double mutant embryos.

The addition of cncB.hs suppresses the rescuing effect seen by DfdScer\UAS.cLa (when driven by Scer\GAL4arm.PS) on Dfd16 mutants. Mouth hooks and cirri are underdeveloped.

Xenogenetic Interactions
Statement
Reference

The mouthhook and cirri defects are rescued in Dfd12/Dfd16 embryos expressing Tcas\DfdScer\UAS.cBa under the control of Scer\GAL469B. Head involution fails in these animals and the posterior mouthparts fail to form.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer

Wakimoto.

Comments
Comments

Strong Dfd allele.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (8)
References (34)