Amino acid replacement: K417M.
Amino acid replacement: K417M. K417 falls in the DEP domain.
A11357603T
K417M | dsh-PA
K417M
Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.
adult thorax & microchaeta
axon & dorsal cluster neuron
egg chamber (with dsh4)
leg & joint
mesothoracic tergum & trichome
ovarian sheath (with dsh4)
dsh1 males show ommatidial chirality phenotypes - planar cell polarity defects -, such as symmetric R3/R4 or R4/R4 configurations.
dsh1 males exhibit reduced fertility in single male crosses (the number of single male crosses that fail to produce offspring is higher than controls) while the number of offspring generated by fertile males is not different when compared to controls.
dsh1 males have short testes with significantly fewer waste bags, fewer normal individualization complexes (ICs) and more abnormal ICs when compared to controls.
dsh1/Y flies are viable and have ommatidial rotation defects (planar cell polarity-like defects) in the eye, as well as mushroom body axon growth and guidance defects.
Dorsal cluster neurons axon branching at the third branching point is unaffected in dsh1 mutant males.
Mutants have ectopic joints in the leg.
The majority of sensory organs of the leg of mutant flies project protrusions indistinguishable in morphology and orientation from those of wild type at 24 hours after puparium formation. However, improperly positioned bract cells and misdirected protrusions from the sensory organs are observed at similar, low frequencies (2.3 +/- 1.4% and 3.0 +/- 1.6%, respectively) and at similar but higher frequencies in the tarsi (8.5 +/- 2.9% and 17.1 +/- 3.3%, respectively) of mutant flies. These errors are not seen in wild type.
dsh1 hemizygotes display ommatidial planar cell polarity (PCP) defects in the eye.
The number of dendritic branches is decreased in the class IV da neurons of homozygous larvae.
dsh1 mutants display PCP defects.
dsh1 maternal/zygotic mutants show little to no epidermal cell shape and denticle alignment defects.
In wild-type wings, the ratio of non-hexagonally shaped cells relative to hexagonally shaped cells is around 11%, whereas in dsh1 homozygous mutant wings this ratio increases to 28%.
Homozygous stage 16 embryos have longer dorsal trunks than normal.
Many dsh1 ommatidia mis-rotate; the variance (s.d.) in degree of rotation is greater than in wild type in adult eyes.
Wing membrane ridges in the anterior region of the wings of wild-type flies have an anterior-posterior orientation, but have a proximal-distal orientation in a dsh1 mutant.
Border cell clusters from dsh1 mutants show an average of 38.4 actin protrusions, reduced compared to the average of 94.8 in wild-type clusters.
Hemizygous males have polarity defects in the wing such as swirling hair patterns and multiple wing hairs.
Hemizygotes show defects in ommatidial polarity.
The orientation of bristles on the nota of dsh1 flies is disturbed.
dsh1 wing hairs show planar polarity defects.
Flies have a planar cell polarity phenotype in the eye.
dsh1/Y mutants have wings with altered planar polarity that contain multiple wing hairs.
Cells in the wing produce double hairs at a frequency of 6.3 +/- 1.0% in dsh1 flies.
Mutant clones in the wing show disruption of polarity, as indicated by wing hairs.
dsh1 mutants show disrupted orientation of wing hairs and random orientation of hairs on the notum.
Mutant animals have a mutant planar cell polarity phenotype.
12% of cells in a defined region of the wing (the ventral surface of the proximal-anterior region) have multiple hairs in hemizygous males. Ectopic F-actin prehairs are seen in pupal wing cells. A hair misorientation phenotype is seen.
Proximal-distal planar polarity is roughly normal at the distal wing tip in the dorsal epidermis of mutant flies.
Legs develop ectopic joints in homozygous flies. These ectopic joints have reversed orientation of the "ball and socket" of the joint and are formed proximal to the normal joints.
dsh1 flies have rough eyes and only 55% of ommatidia have correct rotation and chirality.
Does not affect the eye polarity phenotype of Nact.sev.
Eyes show disruption of ommatidial arrangement in 55.5% of ommatidia, with random or no chirality and misrotations of single ommatidia. The equator is not evident.
Mutants exhibit randomly oriented pI divisions in the epithelial plane. The socket, shaft and pIIb cells are always localised in the region of the cell in contact with pIIb. The socket cells always occupies an eccentric position.
Homozygotes display a strong tissue polarity phenotype. Homozygotes in combination with P{hsfzI} heat shocked to induce a strong hsfz-late phenotype exhibit suppression of the MHC phenotype.
All cells in which planar polarity is evident are affected by dsh mutations. Leg bracts are induced in the wrong position relative to the bristle. Legs show extra mirror image duplications of tarsal joints. Rough eye phenotype reflects abnormal facet packing. Antidromic illumination reveals that although each photoreceptor cluster has the normal trapezoidal arrangement the clusters are misoriented reflecting an abnormal polarity of the whole ommatidium. Some ommatidia are rotated from their normal orientation but have normal handedness. Others are reversed in the A/P or D/V axis.
Most wing cells of homozygous flies form a single hair. Homozygous flies have disruptions in the pattern of wing hair polarity. The prehair initiation site is moved to near the cell centre in pupal wing cells. Double mutants of dsh1 with in1, fy2 or mwh1 resemble in1, fy2 or mwh1 single mutants respectively.
No maternal effect. Hemizygous males and homozygous females have poor viability due to a second site mutation, hemizygous females are fully viable.
Thoracic hairs deranged, males have poor fertility.
Thoracic microchaetae are completely deranged. One or more macrochaetae are abnormally curved. The wings are abnormally inserted, and are usually divergent or slightly upheld. Occasionally one wing is completely deformed, small and blistered. The eyes are ellipsoid with some derangement of the ommatidia.
dsh1 has abnormal neuroanatomy phenotype, enhanceable by DAAM[+]/DAAMEx1
dsh1 has abnormal planar polarity phenotype, enhanceable by CG15283GD13108/Scer\GAL4hs.2sev
dsh1 has abnormal planar polarity phenotype, enhanceable by bdg[+]/bdgGMREP
dsh1 has abnormal planar polarity phenotype, enhanceable by bdg164/bdg[+]
dsh1 has abnormal neuroanatomy phenotype, enhanceable by Wnt5400/Wnt5[+]
dsh1 has abnormal neuroanatomy phenotype, enhanceable by bskDN.UAS/Scer\GAL4ato.3.6
dsh1 has visible | recessive phenotype, enhanceable by sqhA21.Tag:FLAG
dsh1 has abnormal planar polarity | adult stage phenotype, non-enhanceable by Frl[+]/FrlEx83
dsh1 has abnormal neuroanatomy | adult stage phenotype, non-enhanceable by Frl[+]/FrlEx83
dsh1 has abnormal planar polarity | adult stage phenotype, non-enhanceable by Frl[+]/FrlExK62
dsh1 has abnormal neuroanatomy | adult stage phenotype, non-enhanceable by Frl[+]/FrlEx88
dsh1 has abnormal planar polarity | male | adult stage phenotype, suppressible by numb[+]/numb2
dsh1 has abnormal planar polarity | male | adult stage phenotype, suppressible by numb[+]/numb15
dsh1 has visible phenotype, suppressible by Rab5UAS.cEa/Scer\GAL4ap-md544
dsh1 has visible phenotype, suppressible by Scer\GAL4ap-md544/dxΔNBS.UAS
dsh1 has abnormal neuroanatomy phenotype, suppressible by Rac1N17.UAS/Scer\GAL4ato.3.6
dsh1 has abnormal neuroanatomy phenotype, suppressible by hepUAS.cBa/Scer\GAL4ato.3.6
dsh1 has abnormal neuroanatomy phenotype, suppressible by btlDN.UAS/Scer\GAL4ato.3.6
dsh1 has abnormal planar polarity phenotype, suppressible by pk[+]/pk1
dsh1 has visible | recessive phenotype, suppressible by sqhA20.E21
dsh1 has visible | recessive phenotype, suppressible by sqhE20.E21
dsh1 has visible | recessive phenotype, suppressible by sqhE21.Tag:FLAG
dsh1 has visible | recessive phenotype, suppressible by RokαTub84B.PW
dsh1 has abnormal planar polarity | recessive phenotype, suppressible by Scer\GAL4salm-459.2/E(spl)mβ-HLHUAS.cdCa
dsh1 has visible | recessive phenotype, suppressible by Scer\GAL4salm-459.2/E(spl)mβ-HLHUAS.cdCa
dsh1 has abnormal planar polarity | adult stage phenotype, non-suppressible by Frl[+]/FrlEx83
dsh1 has abnormal planar polarity | adult stage phenotype, non-suppressible by Frl[+]/FrlExK62
dsh1 has visible phenotype, non-suppressible by Scer\GAL4ap-md544/dxUAS.cMa
dsh1 has visible phenotype, non-suppressible by Scer\GAL4ap-md544/dxΔPRM.UAS
dsh1 has visible phenotype, non-suppressible by Scer\GAL4ap-md544/dxmRZF.UAS
dsh1 has abnormal planar polarity phenotype, non-suppressible by MbsT541/Mbs[+]
dsh1 has abnormal planar polarity phenotype, non-suppressible by RokαTub84B.PW
dsh1 has abnormal planar polarity phenotype, non-suppressible by sqhE20.E21
dsh1/dsh1 is an enhancer of visible | adult stage phenotype of meru1
dsh1/dsh[+] is an enhancer of abnormal neuroanatomy | adult stage | dominant phenotype of tapGal4
dsh1/dsh[+] is an enhancer of abnormal neuroanatomy phenotype of DAAMEx1
dsh1 is an enhancer of abnormal neuroanatomy phenotype of Scer\GAL4ato.3.6, bskDN.UAS
dsh1/dsh[+] is an enhancer of abnormal neuroanatomy phenotype of Wnt5400
dsh1/dsh[+] is an enhancer of abnormal cell polarity phenotype of fz20/fz19
dsh1/dsh[+] is an enhancer of abnormal planar polarity phenotype of dgo269
dsh1 is an enhancer of visible phenotype of HUAS.cMa, Scer\GAL4GMR.PF
dsh1 is an enhancer of abnormal cell polarity phenotype of Scer\GAL4hs.2sev, pksple.UAS
dsh1 is an enhancer of abnormal cell polarity phenotype of pkpk.sev
dsh1 is an enhancer of abnormal cell polarity phenotype of fz20/fz19
dsh1 is a non-enhancer of abnormal planar polarity | adult stage | somatic clone - Minute background phenotype of FrlEx83
dsh1 is a non-enhancer of abnormal planar polarity | adult stage | somatic clone - Minute background phenotype of FrlExK62
dsh1/dsh[+] is a non-enhancer of abnormal planar polarity phenotype of Scer\GAL4hs.2sev, nmoUAS.cUa
dsh1/dsh[+] is a non-enhancer of visible phenotype of Scer\GAL4en-e16E, kermitGS2053
dsh1/dsh[+] is a non-enhancer of abnormal cell polarity phenotype of Scer\GAL4hs.2sev, shgdCR3h.UAS.sgGFP
dsh1 is a non-enhancer of abnormal neuroanatomy phenotype of Rac1N17.UAS, Scer\GAL4ato.3.6
dsh1 is a non-enhancer of visible phenotype of Scer\GAL4GMR.PF, btlλ.UAS, stumpsUAS.Tag:FLAG
dsh1/dsh[+] is a suppressor | partially of increased mortality during development phenotype of Nab2ex3
dsh1 is a suppressor | partially of abnormal neuroanatomy phenotype of Scer\GAL4ey-OK107, VangUAS.mGFP6
dsh1 is a suppressor | partially of abnormal neuroanatomy phenotype of Scer\GAL4ey-OK107, Wnt5UAS.cFa
dsh1/dsh[+] is a suppressor of abnormal planar polarity phenotype of bdgGMREP
dsh1/dsh[+] is a suppressor of abnormal neuroanatomy phenotype of Scer\GAL4ato.3.6, btlDN.UAS
dsh1/dsh[+] is a suppressor of abnormal planar polarity phenotype of Scer\GAL4hs.2sev, dgoUAS.cFa
dsh1 is a suppressor of abnormal planar polarity | somatic clone | cell non-autonomous phenotype of fz23
dsh1 is a non-suppressor of abnormal planar polarity | adult stage | somatic clone - Minute background phenotype of FrlEx83
dsh1 is a non-suppressor of abnormal planar polarity | adult stage | somatic clone - Minute background phenotype of FrlExK62
dsh1/dsh[+] is a non-suppressor of abnormal neuroanatomy phenotype of DAAMC.UASp, Scer\GAL4ey-OK107
dsh1/dsh[+] is a non-suppressor of abnormal planar polarity phenotype of Scer\GAL4hs.2sev, nmoUAS.cUa
dsh1/dsh[+] is a non-suppressor of visible phenotype of Scer\GAL4en-e16E, kermitGS2053
dsh1/dsh[+] is a non-suppressor of abnormal cell polarity phenotype of Scer\GAL4hs.2sev, shgdCR3h.UAS.sgGFP
dsh1 is a non-suppressor of abnormal neuroanatomy phenotype of Rac1N17.UAS, Scer\GAL4ato.3.6
dsh1 is a non-suppressor of visible phenotype of Scer\GAL4GMR.PF, btlλ.UAS, stumpsUAS.Tag:FLAG
dsh1 is a non-suppressor of abnormal planar polarity | somatic clone | cell autonomous phenotype of fz23
dsh1 is a non-suppressor of abnormal planar polarity | somatic clone | cell non-autonomous phenotype of fz23
dsh1 is a non-suppressor of visible phenotype of Scer\GAL471B, fz::fz2fz2CRD.UAS.Tag:MYC
Rho172O, dsh1 has visible | adult stage phenotype
dsh1/dsh[+], kmr2 has visible | adult stage phenotype
dsh1/dsh[+], kmr2 has abnormal planar polarity | adult stage phenotype
dsh1/dsh[+], kmr1 has visible | adult stage phenotype
dsh1/dsh[+], kmr1 has abnormal planar polarity | adult stage phenotype
Rac1J11/Rac1[+], dsh1 has abnormal neuroanatomy phenotype
dsh1 has adult mushroom body alpha-lobe phenotype, enhanceable by DAAM[+]/DAAMEx1
dsh1 has adult mushroom body beta-lobe phenotype, enhanceable by DAAM[+]/DAAMEx1
dsh1 has ommatidium phenotype, enhanceable by CG15283GD13108/Scer\GAL4hs.2sev
dsh1 has ommatidium phenotype, enhanceable by ed[+]/ed1X5
dsh1 has ommatidium phenotype, enhanceable by bdg[+]/bdgGMREP
dsh1 has ommatidium phenotype, enhanceable by bdg164/bdg[+]
dsh1 has axon & dorsal cluster neuron phenotype, enhanceable by bskDN.UAS/Scer\GAL4ato.3.6
dsh1 has wing hair | increased number phenotype, enhanceable by sqhA21.Tag:FLAG
dsh1 has wing hair | increased number phenotype, enhanceable by Rok2
dsh1 has wing hair | increased number phenotype, enhanceable by zip[+]/zip1
dsh1 has ommatidium phenotype, non-enhanceable by Frl[+]/FrlEx83
dsh1 has axon | adult stage phenotype, non-enhanceable by Frl[+]/FrlEx83
dsh1 has adult mushroom body beta-lobe | adult stage phenotype, non-enhanceable by Frl[+]/FrlEx83
dsh1 has adult mushroom body alpha-lobe | adult stage phenotype, non-enhanceable by Frl[+]/FrlEx83
dsh1 has ommatidium phenotype, non-enhanceable by Frl[+]/FrlExK62
dsh1 has axon | adult stage phenotype, non-enhanceable by Frl[+]/FrlEx88
dsh1 has adult mushroom body beta-lobe | adult stage phenotype, non-enhanceable by Frl[+]/FrlEx88
dsh1 has adult mushroom body alpha-lobe | adult stage phenotype, non-enhanceable by Frl[+]/FrlEx88
dsh1 has ommatidium | male phenotype, suppressible by numb[+]/numb15
dsh1 has ommatidium | male phenotype, suppressible by numb[+]/numb2
dsh1 has leg joint | ectopic phenotype, suppressible by Rab5UAS.cEa/Scer\GAL4ap-md544
dsh1 has leg phenotype, suppressible by Rab5UAS.cEa/Scer\GAL4ap-md544
dsh1 has leg joint | ectopic phenotype, suppressible by Scer\GAL4ap-md544/dxΔNBS.UAS
dsh1 has leg phenotype, suppressible by Scer\GAL4ap-md544/dxΔNBS.UAS
dsh1 has axon & dorsal cluster neuron phenotype, suppressible by Rac1N17.UAS/Scer\GAL4ato.3.6
dsh1 has axon & dorsal cluster neuron phenotype, suppressible by hepUAS.cBa/Scer\GAL4ato.3.6
dsh1 has axon & dorsal cluster neuron phenotype, suppressible by btlDN.UAS/Scer\GAL4ato.3.6
dsh1 has ommatidium phenotype, suppressible by pk[+]/pk1
dsh1 has wing hair | increased number phenotype, suppressible by MbsT541/Mbs[+]
dsh1 has wing hair | increased number phenotype, suppressible by MoeG0323/Moe[+]
dsh1 has wing hair | increased number phenotype, suppressible by ck13
dsh1 has wing hair | increased number phenotype, suppressible by RokαTub84B.PW
dsh1 has wing hair | increased number phenotype, suppressible by ck07130
dsh1 has wing hair | increased number phenotype, suppressible by sqhA20.E21
dsh1 has wing hair | increased number phenotype, suppressible by sqhE20.E21
dsh1 has wing hair | increased number phenotype, suppressible by sqhE21.Tag:FLAG
dsh1 has eye phenotype, suppressible by Rac1hs.sev
dsh1 has ommatidium phenotype, suppressible by Rac1hs.sev
dsh1 has ommatidium phenotype, suppressible by Scer\GAL4salm-459.2/E(spl)mβ-HLHUAS.cdCa
dsh1 has eye phenotype, suppressible by Scer\GAL4salm-459.2/E(spl)mβ-HLHUAS.cdCa
dsh1 has phenotype, suppressible by Rho1V14.sev
dsh1 has ommatidium phenotype, non-suppressible by Frl[+]/FrlEx83
dsh1 has ommatidium phenotype, non-suppressible by Frl[+]/FrlExK62
dsh1 has leg joint | ectopic phenotype, non-suppressible by Scer\GAL4ap-md544/dxUAS.cMa
dsh1 has leg joint | ectopic phenotype, non-suppressible by Scer\GAL4ap-md544/dxΔPRM.UAS
dsh1 has leg joint | ectopic phenotype, non-suppressible by Scer\GAL4ap-md544/dxmRZF.UAS
dsh1 has leg phenotype, non-suppressible by Scer\GAL4ap-md544/dxUAS.cMa
dsh1 has leg phenotype, non-suppressible by Scer\GAL4ap-md544/dxΔPRM.UAS
dsh1 has leg phenotype, non-suppressible by Scer\GAL4ap-md544/dxmRZF.UAS
dsh1 has wing hair phenotype, non-suppressible by RokαTub84B.PW
dsh1 has wing hair phenotype, non-suppressible by sqhE20.E21
dsh1/dsh1 is an enhancer of wing margin bristle phenotype of meru1
dsh1/dsh[+] is an enhancer of adult mushroom body beta-lobe | adult stage phenotype of tapGal4
dsh1/dsh[+] is an enhancer of adult mushroom body alpha-lobe phenotype of DAAMEx1
dsh1/dsh[+] is an enhancer of adult mushroom body beta-lobe phenotype of DAAMEx1
dsh1 is an enhancer of axon & dorsal cluster neuron phenotype of Scer\GAL4ato.3.6, bskDN.UAS
dsh1/dsh[+] is an enhancer of ommatidium phenotype of fz20/fz19
dsh1/dsh[+] is an enhancer of ommatidium phenotype of dgo269
dsh1 is an enhancer of eye phenotype of HUAS.cMa, Scer\GAL4GMR.PF
dsh1 is an enhancer of ommatidium phenotype of Scer\GAL4hs.2sev, pksple.UAS
dsh1 is an enhancer of ommatidium phenotype of pkpk.sev
dsh1 is an enhancer of ommatidium phenotype of fz20/fz19
dsh1 is a non-enhancer of ommatidium | somatic clone - Minute background phenotype of FrlEx83
dsh1 is a non-enhancer of ommatidium | somatic clone - Minute background phenotype of FrlExK62
dsh1/dsh[+] is a non-enhancer of ommatidium phenotype of Scer\GAL4hs.2sev, nmoUAS.cUa
dsh1/dsh[+] is a non-enhancer of wing hair phenotype of Scer\GAL4en-e16E, kermitGS2053
dsh1/dsh[+] is a non-enhancer of ommatidium phenotype of Scer\GAL4hs.2sev, shgdCR3h.UAS.sgGFP
dsh1 is a non-enhancer of axon & dorsal cluster neuron phenotype of Rac1N17.UAS, Scer\GAL4ato.3.6
dsh1 is a non-enhancer of eye phenotype of Scer\GAL4GMR.PF, btlλ.UAS, stumpsUAS.Tag:FLAG
dsh1/dsh[+] is a suppressor | partially of humeral bristle phenotype of Nab2ex3
dsh1 is a suppressor | partially of mushroom body medial lobe phenotype of Scer\GAL4ey-OK107, VangUAS.mGFP6
dsh1 is a suppressor | partially of mushroom body medial lobe phenotype of Scer\GAL4ey-OK107, Wnt5UAS.cFa
dsh1/dsh[+] is a suppressor of ommatidium phenotype of bdgGMREP
dsh1/dsh[+] is a suppressor of axon & dorsal cluster neuron & adult brain phenotype of Scer\GAL4ato.3.6, btlDN.UAS
dsh1/dsh[+] is a suppressor of ommatidium phenotype of Scer\GAL4hs.2sev, dgoUAS.cFa
dsh1 is a suppressor of wing hair | somatic clone | cell non-autonomous phenotype of fz23
dsh1/dsh[+] is a suppressor of wing hair | increased number phenotype of fzI.hs
dsh1 is a non-suppressor of ommatidium | somatic clone - Minute background phenotype of FrlEx83
dsh1 is a non-suppressor of ommatidium | somatic clone - Minute background phenotype of FrlExK62
dsh1/dsh[+] is a non-suppressor of mushroom body phenotype of DAAMC.UASp, Scer\GAL4ey-OK107
dsh1/dsh[+] is a non-suppressor of ommatidium phenotype of Scer\GAL4hs.2sev, nmoUAS.cUa
dsh1/dsh[+] is a non-suppressor of wing hair phenotype of Scer\GAL4en-e16E, kermitGS2053
dsh1/dsh[+] is a non-suppressor of ommatidium phenotype of Scer\GAL4hs.2sev, shgdCR3h.UAS.sgGFP
dsh1 is a non-suppressor of axon & dorsal cluster neuron phenotype of Rac1N17.UAS, Scer\GAL4ato.3.6
dsh1 is a non-suppressor of eye phenotype of Scer\GAL4GMR.PF, btlλ.UAS, stumpsUAS.Tag:FLAG
dsh1 is a non-suppressor of wing hair | somatic clone | cell autonomous phenotype of fz23
dsh1 is a non-suppressor of wing hair | somatic clone | cell non-autonomous phenotype of fz23
dsh1 is a non-suppressor of wing margin bristle | ectopic phenotype of Scer\GAL471B, fz::fz2fz2CRD.UAS.Tag:MYC
dsh1 is a non-suppressor of wing phenotype of Scer\GAL471B, fz::fz2fz2CRD.UAS.Tag:MYC
dsh1/dsh[+], kmr2 has interommatidial bristle phenotype
dsh1/dsh[+], kmr1 has interommatidial bristle phenotype
Rac1J11/Rac1[+], dsh1 has mushroom body phenotype
Presence of FrlEx83/+ or FrlExK62/+ does not significantly alter eye phenotypes seen in dsh1/Y flies. Presence of dsh1/Y (or dsh1/+ females) does not significantly alter eye phenotypes in FrlEx83/FrlEx83 or FrlExK62/FrlExK62 clones. Presence of FrlEx83/+ or FrlEx88/+ does not enhance mushroom body axon growth defects seen in dsh1/Y flies.
One copy of dsh1 enhances the axonal projection defects seen in the α and β lobes of DAAMEx1 mutant mushroom bodies in females. Very few, if any, defects are seen in dsh1 DAAMEx1 double heterozygotes. DAAMEx1 enhances the axon projection phenotypes seen in dsh1/Y males alone. Many of these double hemizygous males exhibit an early growth termination phenotype not seen in either mutant alone.
dsh1 does not suppress the growth and guidance defects seen in the mushroom body axons of flies expressing DAAMC.Scer\UAS.P\T under the control of Scer\GAL4ey-OK107.
Hemizygous dsh1 mutant males with one copy of Rac1J11 exhibit an early growth termination phenotype in the mushroom body axons that is not observed in either dsh1/Y or Rac1J11/+ males alone.
Hemizygous dsh1 partially suppresses the medial lobe fusion phenotype seen when VangScer\UAS.T:Avic\GFP-m6 is expressed in male flies under the control of Scer\GAL4ey-OK107.
Hemizygous dsh1 partially suppresses the medial lobe fusion phenotype seen when Wnt5Scer\UAS.cFa is expressed in male flies under the control of Scer\GAL4ey-OK107.
Expression of Rab5Scer\UAS.cEa under the control of Scer\GAL4ap-md544 partially suppresses the dsh1 ectopic leg joint phenotype, with partial suppression being seen in 80% of the legs examined.
Expression of either dxScer\UAS.cMa, dxΔPRM.Scer\UAS or dxmRZF.Scer\UAS under the control of Scer\GAL4ap-md544 has no effect on the ectopic leg joint phenotype of dsh1 flies.
Expression of dxΔNBS.Scer\UAS under the control of Scer\GAL4ap-md544 in a dsh1 background partially inhibits normal joint formation (this phenotype seen in dxΔNBS.Scer\UAS, Scer\GAL4ap-md544 single mutant) and partially suppresses the formation of ectopic leg joints seen in dsh1 single mutants.
The dsh1 planar cell polarity (PCP) phenotype in the eye is strongly enhanced by the expression of CG15283GD13108 under the control of Scer\GAL4hs.2sev.
Clones expressing fzScer\UAS.T:Avic\GFP-EGFP under the control of Scer\GAL4Act5C.PI in a dsh1 background result in non-clonal cells pointing their trichomes away from the clone. This effect extends at most 3-4 cells from the clone boundary.
A dsh1/+ background does not affect the Scer\GAL4hs.2sev>shgdCR3h.Scer\UAS.T:Avic\GFP-rs ommatidial phenotype.
Wnt5400/+, dsh1/+ double mutants show a more severe dorsal cluster neuron axon phenotype than dsh1/+ single mutants.
Expression of Rac1N17.Scer\UAS, under the control of Scer\GAL4ato.3.6, in a dsh1 background results in a dorsal neuron cluster phenotype similar to when Rac1N17.Scer\UAS is expressed in a wild-type background, which is opposite to that seen in dsh1 mutants.
Expression of hepScer\UAS.cBa, under the control of Scer\GAL4ato.3.6, in a dsh1 background results in a large increase of dorsal cluster neuron axons crossing the optic chiasm, indicating a complete dominance of the hep gain of function phenotype.
Expression of one copy of bskDN.Scer\UAS, under the control of Scer\GAL4ato.3.6, in dsh1 mutant animals results in a synergistic reduction of extension of dorsal cluster neuron axons, with 60% showing no axons crossing the optic chiasm.
Expression of btlDN.Scer\UAS under the control of Scer\GAL4ato.3.6 in a dsh1/+ background suppresses the reduction of dorsal cluster neuron axon extension phenotype seen in either single mutant, with the number of dorsal cluster neuron axons crossing the optic chiasm restored to almost wild-type levels.
The planar cell polarity defects seen in the eyes of flies expressing dgoScer\UAS.cFa under the control of Scer\GAL4hs.2sev are dominantly suppressed by dsh1/+.
The weak planar cell polarity phenotype seen in the eyes of dgo269 homozygotes is enhanced by dsh1/+.
The planar cell polarity phenotype seen in the eyes of dsh1/Y flies is partially suppressed by pk1/+.
ParpGMR.PU shows no genetic interaction with dsh1/+.
The average number of cells having multiple wing hairs in a defined region of the wing (the ventral surface of the proximal-anterior region) is reduced by more than 7-fold in dsh1 males that are also expressing rokαTub84B.PW compared to dsh1 single mutants. The hair misorientation phenotype is not suppressed. Suppression of ectopic F-actin-rich prehairs is seen in double mutant pupae, but the abnormal assembly of the prehair at the centre of the cell is not suppressed. Addition of rok2 to dsh1 mutants results in a 2.5-fold increase in the number of multiple wing hair cells compared to dsh1 single mutants. The dsh1 multiple hair phenotype but not the hair misorientation phenotype is suppressed by sqhE20.E21.
The presence of Rac1hs.sev significantly rescues the dsh1 eye phenotype, increasing the percentage of correctly polarised ommatidia.
The planar polarity defect in dsh1 wings that are expressing fzScer\UAS.cAa under the control of Scer\GAL471B is indistinguishable from the dsh1 single mutant phenotype.
The dsh1 phenotype is partially rescued by HLHmβScer\UAS.cdCa expressed under the control of Scer\GAL4salm-459.2.
Double mutants of VangTbs42, VangA3 or VangA5 with fz24, dsh1 inunspecified and mwhunspecified all showed hair polarity pattern typical of the fz-in group. Multiple hair cell phenotypes of the cell autonomous dsh, in and mwh mutants are epistatic to Vang. Show no interaction with the dominant wing basal cell 1 swirl phenotype of VangTbs42.
dsh1 is rescued by dshST5.EGFP
dsh1 is rescued by dshCK2.hs.Tag:MYC
dsh1 is rescued by dshST1.hs.Tag:MYC
dsh1 is rescued by dshΔBasic.hs.Tag:MYC
dsh1 is rescued by dshCK4.hs.Tag:MYC
dsh1 is rescued by dshfl.hs.Tag:MYC
dsh1 is rescued by dshCK3.hs.Tag:MYC
dsh1 is rescued by dshhs.sev.B
dsh1 is rescued by dshΔDIX.hs.sev
dsh1 is partially rescued by dshTag:FLAG
dsh1 is partially rescued by dshmut6.EGFP
dsh1 is partially rescued by dshST8.EGFP
dsh1 is partially rescued by dshΔPDZ.hs.sev
dsh1 is not rescued by dshY473F.EGFP
dsh1 is not rescued by dshST45.hs.Tag:MYC
dsh1 is not rescued by dshΔDEPD-2.hs.Tag:MYC
dsh1 is not rescued by dshΔPDZ.hs.Tag:MYC
dsh1 is not rescued by dshST124.hs.Tag:MYC
dsh1 is not rescued by dshST4.hs.Tag:MYC
dsh1 is not rescued by dshΔDEP+.UAS/Scer\GAL4hs.PB
dsh1 is not rescued by dshΔbPDZ.UAS/Scer\GAL4hs.PB
dsh1 is not rescued by dshDEP+.hs
dsh1 is not rescued by dshΔC.hs.sev
dsh1 is not rescued by dsh1.hs.sev
dshT:Avic\GFP-EGFP rescues the ectopic leg joint phenotype of dsh1 animals.
dshmut6.T:Avic\GFP-EGFP partially rescues the ectopic leg joint phenotype of dsh1 animals.
dsh1.T:Avic\GFP-EGFP fails to rescue the ectopic leg joint phenotype of dsh1 animals.
Expression of dshY473F.T:Avic\GFP-EGFP is unable to rescue the PCP defects seen in dsh1 mutants.
Expression of dshST8.T:Avic\GFP-EGFP partially rescues the polarity defects of dsh1 mutants; most of the wing hairs show correct polarity but some regions are left with polarity defects.
Suppresses the ommatidial polarity defects seen in dshhs.sev.B.
Fahmy, 1956.
Fahmy.