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General Information
Symbol
Dmel\fz23
Species
D. melanogaster
Name
FlyBase ID
FBal0004939
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
fzR52
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

G14367034A

Reported nucleotide change:

G?A

Amino acid change:

W500term | fz-PA; W500term | fz-PC

Reported amino acid change:

W500term

Comment:

TGG to TGA nonsense mutation at amino acid 500.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: W500term.

There may be a second mutation in the non-translated sequences.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

In fz21/fz23 testes the number of normal individualization complexes (ICs), abnormal ICs and waste bags is similar to controls.

Approximately 15% of fz30/fz23 mutant mushroom bodies exhibit medial lobe fusion phenotypes.

The directional preferences of growing microtubules in fz30/fz23 wing cells is not significantly different from that seen in wild type at 24 hours after puparium formation.

In contrast to wild-types, no clear wing membrane ridge orientation is apparent in either the anterior or posterior region of the wing of fz23/fz21 mutants.

fz23/fz15 mutant clones in the wing non-autonomously disrupt wing membrane ridges.

fz23 mutant clones non-autonomously reorient the wing hairs of neighbouring wild type cells.

Border cell clusters from fz21/fz23 mutants show an average of 38.4 actin protrusions, reduced compared to the average of 94.8 in wild-type clusters.

The hexagonal packing of intervein cells, which usually occurs between wing development stage P2B (when the first morphological signs of veins appear (FBrf0005070), and the middle of P2C (before hair formation (FBrf0005070)) is severely disrupted in fz23/fz23 somatic clones. Hexagonal packing of intervein cells is also partially disrupted over a distance of several cell diameters from these clones.

fz23 has a moderate multiple wing hair phenotype in combination with fz30.

Mutant clones in the wing show domineering non-autonomy: cells distal to the clone, as well as within the clone, show alteration of polarity of wing hairs.

Mutant animals have a mutant planar cell polarity phenotype.

Homozygous fz23 clones in the wing exhibit a non-symmetrical domineering non-autonomy. The polarity defects seen are biased in that cells medial to the clone are more likely to be affected.

Shows domineering non-autonomy of wing hair polarity phenotype in clones in the wing.

Embryos lacking maternal and zygotic fz function (derived from a cross between fz30/fz23 males and females) are missing the RP2/sib lineage in 1-3 hemisegments in 15% of cases. Row 6 neuroblasts are infrequently missing in a partially penetrant manner. Embryos lacking maternal and zygotic fz function (derived from a cross between fz1/fz23 males and females) are missing the RP2/sib lineage in 1-2 hemisegments in 11% of cases.

Non-autonomous phenotype in wing clones. Strong fz phenotype.

Moderate thoracic bristle phenotype; moderate wing-hair disorientation.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
NOT Enhanced by
Statement
Reference

fz23/fz19 has viable phenotype, non-enhanceable by Ror[+]/Ror4

fz23/fz19 has abnormal planar polarity | adult stage phenotype, non-enhanceable by Ror[+]/Ror4

fz23/fz19 has viable phenotype, non-enhanceable by Ror4/Ror4

fz23/fz21 has viable phenotype, non-enhanceable by Ror[+]/Ror4

fz23/fz21 has abnormal planar polarity | adult stage phenotype, non-enhanceable by Ror[+]/Ror4

fz23/fz21 has viable phenotype, non-enhanceable by Ror4/Ror4

fz23 has lethal phenotype, non-enhanceable by Ror[+]/Ror4

fz23 has lethal phenotype, non-enhanceable by Ror4/Ror4

Suppressed by
NOT suppressed by
Statement
Reference

fz23/fz19 has viable phenotype, non-suppressible by Ror[+]/Ror4

fz23/fz19 has abnormal planar polarity | adult stage phenotype, non-suppressible by Ror[+]/Ror4

fz23/fz19 has viable phenotype, non-suppressible by Ror4/Ror4

fz23/fz21 has viable phenotype, non-suppressible by Ror[+]/Ror4

fz23/fz21 has abnormal planar polarity | adult stage phenotype, non-suppressible by Ror[+]/Ror4

fz23/fz21 has viable phenotype, non-suppressible by Ror4/Ror4

fz23 has lethal phenotype, non-suppressible by Ror[+]/Ror4

fz23 has lethal phenotype, non-suppressible by Ror4/Ror4

fz23 has abnormal cell polarity phenotype, non-suppressible by dshhs.PA

Enhancer of
Statement
Reference
NOT Enhancer of
Statement
Reference

fz23/fz19 is a non-enhancer of viable phenotype of Ror4

fz23/fz[+] is a non-enhancer of viable phenotype of Ror4

fz23 is a non-enhancer of abnormal cell polarity phenotype of pkpk.sev

Suppressor of
Statement
Reference

fz23/fz[+] is a suppressor | partially of abnormal planar polarity phenotype of Egfrt1/Egfrf11

fz23/fz[+] is a suppressor | partially of abnormal planar polarity | dominant phenotype of Egfrf24

fz23 is a suppressor of abnormal cell polarity phenotype of dshhs.PA

NOT Suppressor of
Statement
Reference

fz23/fz19 is a non-suppressor of viable phenotype of Ror4

fz23/fz[+] is a non-suppressor of viable phenotype of Ror4

fz23 is a non-suppressor of abnormal cell polarity phenotype of pkpk.sev

Other
Phenotype Manifest In
Enhanced by
Statement
Reference
Suppressed by
NOT suppressed by
Statement
Reference

fz23 has wing hair | somatic clone phenotype, non-suppressible by VangA3

fz23/fz21 has eye phenotype, non-suppressible by fz::fz21-2-2.tub.W

fz23/fz21 has wing hair phenotype, non-suppressible by fz::fz21-2-2.tub.W

fz23/fz21 has eye phenotype, non-suppressible by fz::fz22-1-1.tub.W

fz23/fz21 has wing hair phenotype, non-suppressible by fz::fz22-1-1.tub.W

fz23 has wing hair | somatic clone | cell autonomous phenotype, non-suppressible by dsh1

fz23 has wing hair | somatic clone | cell autonomous phenotype, non-suppressible by mwh1

fz23 has wing hair phenotype, non-suppressible by dshhs.PA

Enhancer of
Statement
Reference

fz23/fz30 is an enhancer of wing hair phenotype of fy2/fy6

fz23 is an enhancer of phenotype of VangTbs42

fz23 is an enhancer of phenotype of VangA3

NOT Enhancer of
Statement
Reference

fz23/fz[+] is a non-enhancer of ommatidium phenotype of Scer\GAL4hs.2sev, nmoUAS.cUa

fz23 is a non-enhancer of ommatidium phenotype of pkpk.sev

Suppressor of
Statement
Reference

fz23/fz[+] is a suppressor | partially of ommatidium phenotype of Egfrt1/Egfrf11

fz23/fz[+] is a suppressor | partially of ommatidium phenotype of Egfrf24

fz23 is a suppressor of wing hair | somatic clone phenotype of VangA3

fz23 is a suppressor of wing hair phenotype of dshhs.PA

NOT Suppressor of
Statement
Reference

fz23/fz[+] is a non-suppressor of ommatidium phenotype of Scer\GAL4hs.2sev, nmoUAS.cUa

fz23 is a non-suppressor of ommatidium phenotype of pkpk.sev

Other
Additional Comments
Genetic Interactions
Statement
Reference

The planar cell polarity defects seen in Egfrf11/Egfrt1 eyes are partially suppressed by fz23/+.

The frequency of rotation defects in the ommatidia of Egfrf24/+ flies is reduced to 12% or 18% respectively if they are also carrying fz23/+.

As is seen with fz23 clones alone, fz23 VangA3 double mutant clones non-autonomously reorient the wing hairs of neighbouring wild type cells so that they point towards the clone.

Expression of fz::fz22-1-1.tub.T:Hsap\MYC fails to rescue the non-autonomous effects of fz23/fz21 mutant clones on the orientation of the wing hairs of neighbouring wild type cells.

A fz23/+ background does not affect the Scer\GAL4hs.2sev>shgdCR3h.Scer\UAS.T:Avic\GFP-rs ommatidial phenotype.

Embryos that lack zygotic fz and fz2 function which are derived from fz23/fzGL31 fz2e2 females mated to fzGL31 fz2e2/+ males are modestly shortened and have mild patterning defects in the cuticle.

When a wing clone is doubly mutant for fz23 and mwh1 or dsh1, domineering nonautonomy of polarity defect of fz23 is unaffected. When a wing clone is doubly mutant for fz23 and mwh1 (or dsh1) in a genetic background deficient for mwh (or dsh1), domineering nonautonomy of polarity defect of fz23 is suppressed, though polarity defect within the clone is not.

The addition of dshhs.PA to fz23 animals has no effect on the fz23 wing hair polarity phenotype.

ParpGMR.PU shows no genetic interaction with fz23/+.

fz23 homozygous clones in pk1 wings, exhibit domineering cell non-autonomy where the direction of the non-autonomy is no longer distal. For these clones the domineering cell autonomy is in the same direction as local hair polarity. fz25 clones in a pkD background show significantly stronger domineering non-autonomy than in a wild-type background. They almost always show proximal domineering non-autonomy. That is cells proximal, but not distal to the clone show abnormal polarity. Thus the direction of the domineering non-autonomy is the same as local hair polarity.

Df(3L)fz-D21 Df(3L)fz2 embryos derived from fz23/Df(3L)fz-D21 Df(3L)fz2 females show a "denticle lawn" cuticle phenotype which is suppressed by expression of fz::fz2fz2CRD.Scer\UAS.T:Hsap\MYC or fz::fz2fzCRD.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4da.G32.

Double mutants with Df(3L)fz2 show variable segmentation defects ranging from a few extra denticles in the posterior part of some segments to complete replacement of naked cuticle with denticles. This phenotype is reminiscent of that for wg loss of function. The expression of armΔN.Scer\UAS driven by Scer\GAL4da.G32 completely overrides this phenotype, causing naked cuticle. Double mutants with Df(3L)fz2 show a reduction of RP2 neurons, though 26% of embryos retain RP2 neurons in 1-3 hemisegments. In double mutants of fz21 or fz23 with Df(3L)fz2 cardiac precursors are missing. Double mutants of fz21 or fz23 with Df(3L)fz2 lack the normal number of midgut constrictions. Overexpression of wgScer\UAS.cLb by Scer\GAL4da.G32 has no effect on the cuticle phenotype of double mutants of fz21 or fz23 with Df(3L)fz2.

The domineering non-autonomy of fz23 clones is suppressed by stan3.

All fz23/fz23; Df(3L)N2-27 double mutant embryos show RP2/sib lineage defects, with 10-13 hemisegments per embryo being affected.

Acts as a dominant enhancer of the dominant wing basal cell 1 phenotype of VangTbs42 and VangA3. VangA3 suppresses the domineering nonautonomy of fz23 clones.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of fzΔSWRNF.tub.T:Hsap\MYC rescues the non-autonomous effects of fz23 mutant clones on the orientation of the wing hairs of neighbouring wild-type cells.

Expression of fztub.T:Hsap\MYC rescues the non-autonomous effects of fz23 mutant clones on the orientation of the wing hairs of neighbouring cells.

Expression of fzΔCRD.tub.T:Hsap\MYC fails to rescue the non-autonomous effects of fz23/fz21 mutant clones on the orientation of the wing hairs of neighbouring wild type cells. 92.3% of clones have surrounding wild type cells pointing towards the clone.

Expression of fztub.T:Hsap\MYC rescues the wing hair phenotype seen in fz21/fz23 mutants.

Expression of fzΔSWRNF.tub.T:Hsap\MYC fails to rescue the wing hair phenotype seen in fz21/fz23 mutants.

Expression of fzΔSKT.tub.T:Hsap\MYC fails to rescue the wing hair phenotype seen in fz21/fz23 mutants.

Expression of fzM469R.tub.T:Hsap\MYC fails to rescue the wing hair phenotype seen in fz21/fz23 mutants.

Expression of fz565Stop.tub.T:Hsap\MYC rescues the wing hair phenotype seen in fz21/fz23 mutants.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer

Adler.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
References (46)