macrochaeta & adult thorax
When a wing clone is doubly mutant for fz23 and mwh1 or dsh1, domineering nonautonomy of polarity defect of fz23 is unaffected. When a wing clone is doubly mutant for fz23 and mwh1 (or dsh1) in a genetic background deficient for mwh (or dsh1), domineering nonautonomy of polarity defect of fz23 is suppressed, though polarity defect within the clone is not.
fz23 homozygous clones in pk1 wings, exhibit domineering cell non-autonomy where the direction of the non-autonomy is no longer distal. For these clones the domineering cell autonomy is in the same direction as local hair polarity. fz25 clones in a pkD background show significantly stronger domineering non-autonomy than in a wild-type background. They almost always show proximal domineering non-autonomy. That is cells proximal, but not distal to the clone show abnormal polarity. Thus the direction of the domineering non-autonomy is the same as local hair polarity.
Df(3L)fz-D21 Df(3L)fz2 embryos derived from fz23/Df(3L)fz-D21 Df(3L)fz2 females show a "denticle lawn" cuticle phenotype which is suppressed by expression of fz::fz2fz2CRD.Scer\UAS.T:Hsap\MYC or fz::fz2fzCRD.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4da.G32.
Double mutants with Df(3L)fz2 show variable segmentation defects ranging from a few extra denticles in the posterior part of some segments to complete replacement of naked cuticle with denticles. This phenotype is reminiscent of that for wg loss of function. The expression of armΔN.Scer\UAS driven by Scer\GAL4da.G32 completely overrides this phenotype, causing naked cuticle. Double mutants with Df(3L)fz2 show a reduction of RP2 neurons, though 26% of embryos retain RP2 neurons in 1-3 hemisegments. In double mutants of fz21 or fz23 with Df(3L)fz2 cardiac precursors are missing. Double mutants of fz21 or fz23 with Df(3L)fz2 lack the normal number of midgut constrictions. Overexpression of wgScer\UAS.cLb by Scer\GAL4da.G32 has no effect on the cuticle phenotype of double mutants of fz21 or fz23 with Df(3L)fz2.
Expression of fzΔCRD.tub.T:Hsap\MYC fails to rescue the non-autonomous effects of fz23/fz21 mutant clones on the orientation of the wing hairs of neighbouring wild type cells. 92.3% of clones have surrounding wild type cells pointing towards the clone.