In contrast to wild-type, gl² mutants fail to exhibit any light-dependent temperature preference.

Mutant flies maintain a high level of activity in an open field arena over the full 10-minute assay (wild-type flies show a decay in locomotor activity over time in this assay).

gl² mutants survive until the late larval period. Ring gland innervation does not occur normally, with aberrant axonal projections and defasciculation. The number of Fmrf-positive axons reaching the corpora allata is reduced.

The precursors of the corpus cardiacum and Bolwig's organ are reduced in number in stage 12 mutant embryos (this phenotype is a dominant effect). At stage 16, the corpus cardiacum is missing in mutant embryos, although the corpus allatum and stomatogastric nervous system are normal.

Homozygous larvae disperse randomly among illuminated and dark quadrants of a petri plate in a visual assay (as do wild-type larvae).

Homozygous third instar larvae show a normal response to UVC radiation. e⁴ gl² mutant larvae show an abnormal response to UVC radiation.

Displays locomotor activity rhythm with an approximately 24h period.

Despite the absence of Bolwig's nerve, bsh expression is normal.

Bolwig's nerves are absent in third larval instar. In place of the normal Bolwig's organ is a loosely clustered group of abnormal neurons with random axonal projections.

Malpighian tubule colour: wild-type.

Cephalic complex smaller than wild type at 36 hr after hatching (27^oC); growth rate subsequently normal.

Mild allele. Slightly less extreme than gl¹. Eyes reduced to two-thirds normal size, ovoid, glassier and smoother than gl¹. Eye colour: blotchy scarlet in both sexes with rim and eroded patches of colorless material. Ocellus colour: colorless and papillose.

gl² is a non-enhancer of phenotype of Doa⁷/Doa^HD

gl² is a non-enhancer of phenotype of Doa^HD/Doa^Dem

gl² is a non-suppressor of phenotype of Doa⁷/Doa^HD

gl² is a non-suppressor of phenotype of Doa^HD/Doa^Dem