FlyBase Allele Report: Dmel\cac[HC129]

General Information

Symbol

Dmel\cac^HC129

Species

D. melanogaster

Name

FlyBase ID

FBal0006138

Feature type

allele

Associated gene

Dmel\cac

Associated Insertion(s)

Carried in Construct

Also Known As

l(1)L13^HC129

Key Links

Allele class

loss of function allele

Mutagen

X ray

Nature of the Allele

Allele class

loss of function allele

(Frank et al., 2009)

Mutagen

X ray

(Kulkarni and Hall, 1987)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Cytology

Nature of the lesion

Statement

Reference

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Huntington's disease

modeled by Hsap\HTT^128Q.FL.UAS

(Romero et al., 2008)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/larval neuromuscular junction | larval stage, with Scer\GAL4^elav-C155, cac^RQ.UAS.EGFP

(Brusich et al., 2018)

larval multidendritic class IV neuron | P-stage | somatic clone

(Kanamori et al., 2013)

Detailed Description

Statement

Reference

In CNS-removed larvae that are both mutant for cac^HC129 and express cac^RQ.UAS.EGFP under the control of Scer\GAL4^elav-C155, 30 presynaptic pulses lead to NMJ EPSP profiles with shoulders.

(Brusich et al., 2018)

cac^HC129 mutant embryos exhibit normal amplitude mEJPs but severely reduced synaptic transmission. No evoked response can be detected in 1mM Ca[2+] Saline, compared to an average response of over 1nA in wild-type embryos.

cac^HC129 mutant embryos develop boutons and exhibit normal Ank2 localisation.

(Kurshan et al., 2009)

Song pulse amplitude in heterozygotes with cac¹ is higher than wild type.

(Kulkarni and Hall, 1987)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

cac^HC129 has abnormal neurophysiology | somatic clone | P-stage phenotype, enhanceable by Ca-α1D^X10/Ca-α1D^X10

(Kanamori et al., 2013)

Suppressed by

Statement

Reference

cac^HC129 has viable phenotype, suppressible by Frq2[+]/Frq2^f06131

(Dason et al., 2009)

Enhancer of

Statement

Reference

cac^HC129/cac^HC129 is an enhancer of abnormal neurophysiology | somatic clone | P-stage phenotype of Ca-α1D^X10

(Kanamori et al., 2013)

NOT Enhancer of

Statement

Reference

cac^HC129/cac[+] is a non-enhancer of abnormal neurophysiology | recessive | third instar larval stage phenotype of GluRIIA^SP16

(Frank et al., 2009)

Suppressor of

Statement

Reference

cac^HC129 is a suppressor of viable phenotype of Frq2^f06131

(Dason et al., 2009)

cac^HC129/cac[+] is a suppressor of abnormal neurophysiology phenotype of Hsap\HTT^128Q.FL.UAS, Scer\GAL4^elav-C155

(Romero et al., 2008)

NOT Suppressor of

Statement

Reference

cac^HC129/cac[+] is a non-suppressor of abnormal neurophysiology | recessive | third instar larval stage phenotype of GluRIIA^SP16

(Frank et al., 2009)

Other

Statement

Reference

Ca-α1D^X10, cac^HC129 has abnormal neuroanatomy | somatic clone | P-stage phenotype

(Kanamori et al., 2013)

Df(1)frq^del1, cac^HC129 has abnormal neurophysiology phenotype

(Dason et al., 2009)

Exn[+]/Exn^unspecified, GluRIIA^SP16, cac^HC129/cac[+] has abnormal neurophysiology | recessive | third instar larval stage phenotype

(Frank et al., 2009)

Phenotype Manifest In

Enhanced by

Statement

Reference

cac^HC129 has larval multidendritic class IV neuron | somatic clone | P-stage phenotype, enhanceable by Ca-α1D^X10/Ca-α1D^X10

(Kanamori et al., 2013)

Enhancer of

Statement

Reference

cac^HC129/cac^HC129 is an enhancer of larval multidendritic class IV neuron | somatic clone | P-stage phenotype of Ca-α1D^X10

(Kanamori et al., 2013)

NOT Enhancer of

Statement

Reference

cac^HC129/cac[+] is a non-enhancer of embryonic/larval neuromuscular junction phenotype of GluRIIA^SP16

(Frank et al., 2009)

NOT Suppressor of

Statement

Reference

cac^HC129/cac[+] is a non-suppressor of embryonic/larval neuromuscular junction phenotype of GluRIIA^SP16

(Frank et al., 2009)

Other

Statement

Reference

Ca-α1D^X10, cac^HC129 has dendrite | somatic clone | P-stage phenotype

(Kanamori et al., 2013)

Exn[+]/Exn^unspecified, GluRIIA^SP16, cac^HC129/cac[+] has embryonic/larval neuromuscular junction phenotype

(Frank et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Heterozygosity for cac^HC129 significantly suppresses the increase in evoked excitatory junctional potential currents (EJCs) and quantal content (QC) in homozygous Df(2R)KT40 mutants.

(Tsurudome et al., 2010)

Flies trans-heterozygous for Frq2^f06131 and cac^HC129 exhibit a 50% reduction in viability.

There is a significant reduction in EJP amplitude in Df(X)frq^del1/cac^HC129 larvae compared to controls. mEJP frequency and amplitudes are not significantly different.

(Dason et al., 2009)

The presence of the heterozygous cac^HC129/+ mutation has little effect on the quantal content in a homozygous GluRIIA^SP16 background.

The recessive GluRIIA^SP16 single mutant NMJ synaptic homeostatic response is completely suppressed in the presence of a heterozygous cac^HC129/+ mutation in combination with an Exn^unspecified/+ heterozygous genetic background.

(Frank et al., 2009)

Xenogenetic Interactions

Statement

Reference

cac^HC129/+ significantly suppresses the increased excitatory junction potential (EJP) amplitude and decreased failure rate seen in Hsap\HD^{128Q.FL.Scer\UAS}, Scer\GAL4^elav-C155 animals at 0.25 mM Ca[2+].

(Romero et al., 2008)

Complementation and Rescue Data

Fails to complement

cac¹

(Kulkarni and Hall, 1987)

Rescued by

cac^HC129 is rescued by Scer\GAL4^elav-C155/cac^UAS.EGFP

(Kawasaki et al., 2004)

Partially rescued by

cac^HC129 is partially rescued by cac^RQ.UAS.EGFP/Scer\GAL4^elav-C155

(Brusich et al., 2018)

cac^HC129 is partially rescued by Scer\GAL4^elav-C155/cac^UAS.cKa

(Kawasaki et al., 2002)

Not rescued by

cac^HC129 is not rescued by Scer\GAL4^elav-C155/cac^SL.UAS.EGFP

(Brusich et al., 2018)

cac^HC129 is not rescued by Scer\GAL4^elav-C155/cac^{RQ,SL.UAS.EGFP}

(Brusich et al., 2018)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

8579

P{GawB}elav^C155 cac^HC129 sd¹ f¹/FM7i, P{ActGFP}JMR3

Notes on Origin

Discoverer

Lefevre.

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

1(1)_L13^HC129

(Kuromi et al., 2010)

Dmca1A⁶

(Rieckhof et al., 2003)

Dmca1A^HC129

(Romero et al., 2008)

cac^HC129

(Brusich et al., 2018, Brusich et al., 2015, Wiemerslage and Lee, 2015, Kanamori et al., 2013, Popodi et al., 2010-, Tsurudome et al., 2010, Dason et al., 2009, Frank et al., 2009)

cac^null

(Kuromi et al., 2010)

cacL13^HC129

(Kurshan et al., 2009)

l(1)11Aa⁶

l(1)HC129

l(1)L13^HC129

(Kawasaki and Ordway, 2009, Venken et al., 2009, Kawasaki et al., 2004, Kawasaki et al., 2002, Kulkarni and Hall, 1987)

nbA^HC129

Name Synonyms

Secondary FlyBase IDs

References (17)

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