Double heterozygotes with Eip74EFneo24 or Eip74EFDL-1 show no effect on development. More than 25% of Eip74EFneo24 carrying a single effective dose of br+ formed a misshapen puparium. This phenotype is 100% penetrant in a br2Bc-2 mutant background. Most double mutants die during the prepupal stage, earlier than either br2Bc-2 or Eip74EFneo24 mutants alone. In 16% of double mutants eye development proceeds to the point where pigment can be observed, but other discs do not undergo morphogenesis. In double mutants with Eip74EFDL-1 the lethal phase shifts earlier to the prepupal stage, and the puparium is extremely curved.
Expression of the brBRcore.NS.Z3.hs transgene partially rescues the malrotated genitalia phenotype of br2Bc-2 males; this is inversely related to temperature, with 40% of flies showing the phenotype at 22-23oC and only 14% showing the phenotype at 25oC.
br2Bc-2 animals are rescued by expression of brBRcore.NS.Z3.hs during larval development. Rescued flies that eclose have wild-type legs and wings and live for several days as fertile adults. br2Bc-2 animals are partially rescued by expression of brZ2.hs during larval development. Rescued flies that eclose have malformed wings and legs and die very soon after eclosing. br2Bc-2 animals are not rescued by expression of brBRcore.Q1.Z1.hs or brBRcore.TNT.Q1.Z1.hs during larval development. brBRcore.Z4.hs has a small effect on br2Bc-2 when expressed using 37oC heat shocks during larval development; some animals develop into late pupal stages, and very rarely, very malformed animals eclose.