pole cell & cytoskeleton | germ-line clone
Embryos that are both maternally and zygotically mutant for RhoGEF204291 and are also heterozygous or homozygous for Gef64C1 show a mild increase in the frequency of spiracle defects compared to embryos that are both maternally and zygotically mutant for RhoGEF204291 in a Gef64C+ background.
Embryos derived from RhoGEF204291 germ-line clones that are also zygotically homozygous for Df(3R)tll-e resemble RhoGEF204291 single mutants (derived form germ-line clones) during syncytial divisions. However, RhoGEF204291, Df(3R)tll-e double mutants show a progressive deterioration in their actin networks during cellularization: they have breaks in actin rings more frequently than the single mutants; toward the end of the slow phase of cellularization, their actin network is severely fragmented with only isolated actin rings being found (although these isolated rings did not constrict prematurely, as is observed in Df(3R)tll-e embryos); and at later stages of cellularization, the actin network network is completely disrupted.
RhoGEF204291/+ suppresses the mutant wing phenotype caused by expression of LIMK1Scer\UAS.cCa under the control of Scer\GAL4en-e16E (the % of wings with normal morphology at 18oC is increased from 9% to 87%).
RhoGEF204291 shows a weak interaction (5-24% of double heterozygotes have at least one malformed leg) with the following mutations: Sb63b and Sb70. br1 fails to show a significant genetic interaction (assayed in terms of a malformed leg phenotype) with RhoGEF204291. Sbsbd-201/Sbsbd-1 shows a weak interaction (5-24% of double mutants have at least one malformed leg) with the following mutations: RhoGEF204291/+.
The fraction of flies showing a malformed leg phenotype in at least one leg, for RhoGEF204291 in double heterozygous combination with one of the following alleles is - SbEbr20 : 1%, SbEbr48 : 2%, SbEbr228 : 4%, SbEbr448 : 4%, SbEbr536 : 2%, SbEbr623 : 0%, Rho1Ebr233 : 4%, Rho1Ebr246 : 17%, bsEbr292 : 0%, E(br)24Ebr24 : 2%, E(br)65Ebr65 : 4%, E(br)155Ebr155 : 6%, E(br)165Ebr165 : 7%, E(br)333Ebr333 : 9%, E(br)72Ebr72 : 1%, E(br)121Ebr121 : 49%, E(br)160Ebr160 : 2%, E(br)187Ebr187 : 2%, E(br)420Ebr420 : 3% and E(br)444Ebr444 : 36%.