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General Information
Symbol
Dmel\dally06464
Species
D. melanogaster
Name
FlyBase ID
FBal0009563
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
dallyP2, dally-lacZ, l(3)06464
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Insertion components
P{PZ}dally06464
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The ovarioles of dally06464/dally80 females show progressive loss of the germline stem cells (GSCs), with 59% of ovarioles containing no GSCs in the niche at day 10 after eclosion.

Wings of homozygous adults are smaller than normal, primarily due to a reduction in cell number rather than cell size.

dally06464 mutants display ISNb bypass at a similar rate to wild-type embryos.

Homozygotes have rough eyes, a reduced antenna, incomplete wing vein 5 and reduced genitalia, with incomplete penetrance. The ability of the lamina precursor neurons to enter a second division cycle is reduced in homozygous larvae.

The number of the multiply innervated (recurved) bristles in the dorsal row of dally06464/dallygem wings decreases to 55% of that of wild-type flies. Homozygotes exhibit defects the formation of macrochaetae. They lack anterior dorsocentral (aDC) and posterior supralar (pSA) bristles. the posterior dorsocentral (pDC) is displaced anteriorly or forms at an intermediate position between the aDC and pDC. Homozygotes also defects in microchaetae. Numbers are reduced and occur irregularly. In dallygem/dally06464 flies, 86% of animals lack the posterior supraalar bristle (pSA), 24% the anterior dorsocentral bristles (aDC) and 1% the posterior supraalar bristle (pSA).

3% of homozygous dally06464 flies exhibit a wing notching phenotype.

dallyΔP-527/dally06464 mutant embryos show a loss of naked cuticle and abnormalities with segment polarity (21% penetrance). About 70% of dally06464/dallygem flies show abnormalities in their genitalia.

Semilethal. Reduction or complete loss of genitalia, disordering and reduction in the number of ommatidia, reductions and duplications of the antenna and incomplete wing vein V and wing notching. Disruptions in the cell cycle progression of lamina precursors and of G2-M progression in the eye disc.

Homozygotes have wing vein defect.

some visible defects in eyes, wing veins, genitalia, male sterile

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

dally06464 has visible | recessive phenotype, enhanceable by wg[+]/wgl-8

dally06464 has visible | recessive phenotype, enhanceable by dppd-blk/dpp[+]

dally06464 has visible | recessive phenotype, enhanceable by dppd14/dpp[+]

NOT Enhanced by
Statement
Reference

dally06464 has partially lethal phenotype, non-enhanceable by Df(2R)59AB/+

Suppressed by
Statement
Reference

dally06464 has visible phenotype, suppressible | partially by CycA3/CycA[+]

dally06464 has partially lethal phenotype, suppressible by CycA3/CycA[+]

dally06464 has visible phenotype, suppressible | partially by CycA[+]/CycA5

dally06464 has partially lethal phenotype, suppressible | partially by CycA[+]/CycA5

dally06464 has visible | recessive phenotype, suppressible by dppd14/dpp[+]

NOT suppressed by
Statement
Reference

dally06464 has partially lethal phenotype, non-suppressible by Df(2R)59AB/+

Enhancer of
Statement
Reference
NOT Enhancer of
Statement
Reference

dally[+]/dally06464 is a non-enhancer of visible | dominant phenotype of sogEP7

dally[+]/dally06464 is a non-enhancer of visible | dominant phenotype of sogEP11

Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference

dally[+]/dally06464 is a non-suppressor of visible | dominant phenotype of sogEP11

dally[+]/dally06464 is a non-suppressor of visible | dominant phenotype of sogEP7

Other
Statement
Reference
Phenotype Manifest In
Enhanced by
Statement
Reference
NOT Enhanced by
Statement
Reference

dally06464 has antenna phenotype, non-enhanceable by Df(2R)59AB/+

dally06464 has genitalia phenotype, non-enhanceable by Df(2R)59AB/+

dally06464 has ommatidium phenotype, non-enhanceable by Df(2R)59AB/+

dally06464 has wing vein L5 phenotype, non-enhanceable by Df(2R)59AB/+

dally06464 has genitalia phenotype, non-enhanceable by CycA[+]/CycAunspecified

Suppressed by
Statement
Reference

dally06464 has antenna phenotype, suppressible | partially by CycA3/CycA[+]

dally06464 has lamina anlage & mitotic cell cycle phenotype, suppressible | partially by CycA3/CycA[+]

dally06464 has ommatidium phenotype, suppressible by CycA3/CycA[+]

dally06464 has wing vein L5 phenotype, suppressible | partially by CycA3/CycA[+]

dally06464 has antenna phenotype, suppressible | partially by CycA[+]/CycA5

dally06464 has ommatidium phenotype, suppressible by CycA[+]/CycA5

dally06464 has wing vein L5 phenotype, suppressible | partially by CycA[+]/CycA5

dallygem/dally06464 has genitalia phenotype, suppressible by wg[+]/wgl-17

dally06464 has wing phenotype, suppressible by dppd14/dpp[+]

NOT suppressed by
Statement
Reference

dally06464 has genitalia phenotype, non-suppressible by Df(2R)59AB/+

dally06464 has lamina anlage & mitotic cell cycle phenotype, non-suppressible by Df(2R)59AB/+

dally06464 has ommatidium phenotype, non-suppressible by Df(2R)59AB/+

dally06464 has wing vein L5 phenotype, non-suppressible by Df(2R)59AB/+

dally06464 has genitalia phenotype, non-suppressible by CycA[+]/CycAunspecified

dally06464 has lamina anlage & mitotic cell cycle phenotype, non-suppressible by stg2/stg2/stg[+]

dally06464 has antenna phenotype, non-suppressible by Df(2R)59AB/+

Enhancer of
Statement
Reference
NOT Enhancer of
Statement
Reference

dally06464 is a non-enhancer of anterior fascicle & axon | ectopic phenotype of Larbypass

dally[+]/dally06464 is a non-enhancer of wing vein phenotype of sogEP7

dally[+]/dally06464 is a non-enhancer of wing vein phenotype of sogEP11

Suppressor of
Statement
Reference

dally06464 is a suppressor | partially of phenotype of wgPE2

NOT Suppressor of
Statement
Reference

dally[+]/dally06464 is a non-suppressor of wing vein phenotype of sogEP11

dally[+]/dally06464 is a non-suppressor of wing vein phenotype of sogEP7

Other
Statement
Reference

dally[+]/dally06464, dppd12 has eye phenotype

dally[+]/dally06464, dppd-blk has eye phenotype

dally[+]/dally06464, dppd5 has eye phenotype

dally[+]/dally06464, dppd5 has antenna phenotype

Additional Comments
Genetic Interactions
Statement
Reference

The dally06464 mutation does not increase the frequency of the Larbypass ISNb bypass phenotype.

The lethality seen in dally06464 homozygotes is reduced if they are also heterozygous for CycA5. The penetrance of the eye, antenna and wing defects seen in dally06464 homozygotes is reduced if they are also heterozygous for CycA5. The lethality seen in dally06464 homozygotes is completely suppressed if they are also heterozygous for CycA3. The penetrance of the eye, antenna and wing defects seen in dally06464 homozygotes is reduced if they are also heterozygous for CycA3. The severity of the dally06464 eye defects are also reduced in the double mutants. The ability of dally06464 lamina precursor neurons to enter a second division cycle is largely restored if they are also carrying CycA3. Df(2R)59AB has no effect on the divisions of dally06464 lamina precursor neurons. One copy of stg2 does not rescue the second division in dally06464 lamina precursor neurons and appears to expand the first G2/M domain of lamina precursor neuron division.

The addition of wgl-17 to dallygem/dally06464 flies, leads to enhancement of the posterior supraalar bristle phenotype seen in dallygem/dally06464 flies alone: 30% of flies have the phenotype, rather than 1%. The addition of put10460 to dallygem/dally06464 flies, leads to enhancement of the anterior dorsocentral bristle phenotype seen in dallygem/dally06464 flies alone: 90% of flies have the phenotype, rather than 24%. The posterior supraalar bristle phenotype is also enhanced by the addition of put10460. All flies have the phenotype, rather than 86%.

Homozygous embryos derived from dally06464 germline clones show weak segment polarity defects (8% penetrance). More severe defects are detected in embryos where the maternal sfl message is also eliminated (14% penetrance). The segment polarity phenotype seen in embryos derived from dally06464 germline clones is enhanced if zygotic wg is reduced by half. Penetrance is increased from 8% to 16%. Wings on wgl-8/+; dally06464/dally06464 animals show a stronger wing notch phenotype than dally06464/dally06464 alone at a higher penetrance (3% to 8%). dally06464/dally06464 strongly enhances the fully penetrant mild wing margin defect see when ectopic expression of fz2Scer\UAS.N at the presumptive wing margin is driven by Scer\GAL4C96. The wing margin phenotype seen in dally06464/dally06464, is fully suppressed by ectopic expression of armact.Scer\UAS driven by Scer\GAL4C96. The ectopic bristle phenotype seen when fz2Scer\UAS.cCa is driven uniformly in the wing pouch by Scer\GAL469B is strikingly reduced in fz2Scer\UAS.cCa, dally06464/Scer\GAL469B, dally06464 flies.

The abnormal genitalia phenotype of dally06464/dallygem flies is dominantly suppressed (down to less than 10% penetrance) by wgl-17.

Flies heterozygous for both dally06464 and dppd-blk show eye abnormalities never observed in flies heterozygous for dally06464 or dppd-blk alone. dally06464 homozygotes show a slight reduction in the eye which is more severe when they are also heterozygous for dppd-blk. The penetrance and expressivity of eye, antenna and genitalia defects seen in dally06464 homozygotes is increased if they are also heterozygous for dppd14. The wing defects seen in dally06464 homozygotes are suppressed if they are also heterozygous for dppd14. The penetrance of eye, antenna and genitalia defects seen in dally06464 homozygotes is decreased if they are carrying three copies of dpp+. The wing defects seen in dally06464 homozygotes are enhanced if they are carrying three copies of dpp+.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer

A. Spradling.

Separable from: l(3)87Df06464. Evidence suggests that the "l(3)06464" line contains 2 P{PZ} insertions; one at 87D in l(3)87Df (P{PZ}l(3)87Df06464) corresponding to the site of the flanking sequence AQ073333, and one at 66E (P{PZ}dally06464) which causes a mutation in dally.

Comments
Comments

Complements: l(3)0162901629. Complements: l(3)0626406264. Complements: mRpL1210534. Complements: mRpL12j4B2. Complements: l(3)neo141. Complements: mRpL12rL424. Complements: l(3)s2383s2383.

Reversion of the P-element insertion provides evidence that the insertion is responsible for the mutant phenotype.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (7)
References (24)