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General Information
D. melanogaster
FlyBase ID
Feature type
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
de2f1rm729, E2Frm729, dE2F1729, dE2F729, E2f729, E2F-lacZ
Key Links
Nature of the Allele
Mutations Mapped to the Genome
Additional Notes
Associated Sequence Data
DNA sequence
Protein sequence
Progenitor genotype
Nature of the lesion

P{PZ} is inserted near to the translation start site, such that the Ecol\lacZ coding region is in the same orientation as E2f.

P{PZ} is inserted 48 nucleotides upstream of the initiator methionine, with the Ecol\lacZ in the same orientation as E2f.

Insertion components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Modifiers Based on Experimental Evidence ( 0 )
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
Disease-implicated variant(s)
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description

E2f1rM729/E2f1rM729 somatic clones of terminal tracheal cells are smaller in size, have reduced nuclei, less branches and show gas filling defects compared to wild-type controls in third instar larvae. Heterozygous neighboring stalk cells invade the small mutant terminal cells leading to the formation of two or more adherens junctions (not one as in wild-type controls ) between them and show a compensatory increase in cell size.

Homozygous clones in the developing optic lobe are very small. Some of the mutant cells are extruded from the neuroepithelium and basally enter the medulla cortex, where ectopic neuroblasts form prematurely.

E2f1rM729 germline stem cell (GSC) clones behave similarly to control GSC clones and do not dramatically affect GSC maintenance.

Homozygous clones in the eye disc are very small.

E2frM729 eye disc clones in a Minute/+ background are extremely small.

E2f91/E2frM729 transheterozygous larvae, are much smaller than wild-type, 5 days after egg laying. They fail to develop into 3rd instar larvae.

E2f91/E2frM729 transheterozygotes can develop into the late second instar or early third instar larval stage at a very slow pace (around 13 days after egg laying), but do not develop into pupae. BrdU incorporation is not seen in the optic lobe region of the brain in day 13 E2f91/E2frM729 larvae, in contrast to wild-type third instar larvae. The eye discs of E2f91/E2frM729 larvae are very small and have only a few cells labelled with BrdU with no specific pattern.

Cells in homozygous clones induced in the wing pouch of the wing disc become enlarged and are gradually lost from the disc epithelium.

Clones induced in the eye in the first or second larval instar are not detected in the adult eye. They can be detected in the eye disc, but are much smaller than the adjacent twin spots, with fewer cells. These cells have initiated neuronal differentiation. Resulting ommatidia have incomplete sets of photoreceptors, and photoreceptors that are present often have abnormal morphology. Rhabdomeres are small in diameter, the rhabdomeres of the outer photoreceptors may project centrally and the photoreceptors do not extend the full length of the retina.

Cells in the CNS that normally enter endocycles fail at S17. Replication either fails or occurs at a very low rate.

External Data
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Enhancer of
NOT Enhancer of
Suppressor of
NOT Suppressor of
Phenotype Manifest In
Suppressed by

E2f191/E2f1rM729 has eye disc phenotype, suppressible by Rbf14/Rbf120a

E2f191/E2f1rM729 has eye disc & mitotic cell cycle phenotype, suppressible by Rbf14/Rbf120a

NOT suppressed by
Enhancer of
Suppressor of
Additional Comments
Genetic Interactions

The high levels of apoptosis (detected by Caspase-3 staining) observed in Rbf15aΔ;Stam19 double homozygous somatic clones in third instar larval eye disc is completely abolished when the clones are created in E2f1[i2]/E2f1[rM729] mutant background.

An E2f1rM729 mutant background dominantly enhances the Scer\GAL4GMR.PF>lin-52GD12885 eye phenotype. This is accompanied by a corresponding increase in hid protein and elevated apoptosis in the eye-antennal disc.

Tsc1f01910 E2frM729 double mutant eye disc clones in a wild-type background are smaller than Tsc1f01910 single mutant eye disc clones.

When Tsc1f01910 E2frM729 double mutant clones are generated in Rbf120a, the sizes of the Tsc1f01910 E2frM729 double mutant clones are much smaller than that of Tsc1f01910 single mutant clones.

The prevailing cell death phenotype observed in Rbf120a Tsc1f01910 double mutant clones at the morphogenetic furrow is no longer present in Rbf120a E2frM729 Tsc1f01910 triple mutant cells, indicating that the increased level of ectopic cell death observed in Rbf120a Tsc1f01910 double mutant cells is E2f-dependent.

Each of l(3)mbt6a14, l(3)mbt4d30, l(3)mbt3d23, l(3)mbt5d8, l(3)mbt4d16, l(3)mbt5d6, l(3)mbt6a31, l(3)mbt3d33, Doa6d2, Doa6a21, Doa5d7, gfzf6a27, B525d21, B523d16, B523d24, CG311337d21, CG31133f07858, bel7d19, Su(E1)-A7d13, Su(E1)-A6a39, Su(E1)-B4d26, Su(E1)-B6a11, Su(E1)-C1d24, Su(E1)-C3d28, Su(E1)-D6a47, Su(E1)-E3d25, Su(E1)-F6b9, Su(E1)-G6a3, Su(E1)-H6a50, Su(E1)-I6d9, Su(E1)-J6a8, Su(E1)-K6b12, Su(E1)-L6b19, Su(E1)-M6b31, Su(E1)-N4d22 and Su(E1)-O6a19 can suppress the proliferation block of E2frM729 clones, allowing recovery of double mutant clones in the eye.

The second mitotic wave is strongly restored in l(3)mbt3d33 E2frM729 and Su(E1)-A7d13 E2frM729 double mutant clones in the eye disc.

The second mitotic wave is weakly rescued in CG311337d21 E2frM729 and gfzf6a27 E2frM729 double mutant clones in the eye disc, with the double mutant cells entering the second mitotic wave several columns more posterior than in wild-type cells. In addition BrdU incorporation persists several columns more posterior in the double mutant cells than in the adjacent wild-type cells.

The second mitotic wave is weakly rescued in Doa6d2 E2frM729 double mutant clones in the eye disc, with double mutant cells only being sporadically labelled with BrdU in the second mitotic wave. Those double mutant cells that do incorporate BrdU are always found to be significantly more than adjacent wild-type cells that incorporate BrdU.

The shiny-eye and multiple photoreceptor-R8 phenotype seen in rno1, Rbf15aΔ eye somatic mutant clones is significantly suppressed in a E2fi2/E2frM729 mutant background.

bamΔ86 E2f1rM729 double mutant germline stem cell (GSC) clones are much less competitive than bamΔ86 single mutant GSCs.

Relatively large E2f2c03344 E2frM729 double mutant clones can be recovered in the eye disc.

The small size of E2frM729 clones in the eye disc is partially rescued if they are also mutant for either bel7d19, belL4740 or smo1.

Scer\GAL4GMR.PF-driven expression of Su(H)Scer\UAS.T:Hsim\VP16 is unable to rescue the small size of E2frM729 eye disc clones.

In wild-type animals, exposure to 40Gy of γ rays induces a distinct pattern of apoptosis in the wing disc, focussed on the the wing pouch, but excluding the DV boundary region and prospective vein. In E2frM729/E2f91; E2f276Q1/E2f2g5 animals, the same irradiation treatment induces a different pattern of apoptosis a smaller number of apoptotic cells are clustered in the center of the wing pouch, many in the D-V boundary region or prospective veins.

The addition of E2f276Q1/Df(2L)G5.1 to E2f91/E2frM729 flies almost completely suppresses the E2f91/E2frM729 phenotypes - mutants develop normally without any significant delay in larval growth, reach pupal stage and finally die as mid- or late pupae. The pattern of DNA synthesis in eye discs is largely normal. The addition of Dpa2/Df(2R)vg-B to E2f91/E2frM729 flies almost completely suppresses the E2f91/E2frM729 phenotypes - mutants develop normally without any significant delay in larval growth.

The rough eye phenotype seen upon expression of DrefScer\UAS.cSa in the developing eye under the control of Scer\GAL4GMR.PS is suppressed in an E2frM729 heterozygous background.

Approximately 50% of the expected number of Rbf14/Y ; E2fi2/E2frM729 flies survive to the adult stage, and the development of these flies is not significantly delayed compared to sibling flies. The surviving flies have normal eyes, normal macrochaetae on the notum and normal wings and legs. The larval lethality of E2f91/E2frM729 animals can be suppressed by Rbf120a/Rbf14; the larvae develop into large late third instar larvae and many can pupate. Some Rbf120a/Rbf14 ; E2f91/E2frM729 animals can develop into pharate adults with adult eyes, legs and wing structures. The development of these larvae is significantly retarded compared to wild-type larvae, with the earliest pupae observed at around day 11 after egg laying. The normal pattern of DNA replication in the optic lobe region is restored in E2f91/E2frM729 larvae that are also carrying Rbf120a/Rbf14. In Rbf120a/Rbf14 ; E2f91/E2frM729 eye discs the overall pattern of DNA replication is normal. No significant cell death is seen in these eye discs. In late third instar discs there are about 25 rows of developing photoreceptor cells (as in wild-type discs) but there are much fewer cells anterior to the morphogenetic furrow than in wild type.

Dominantly enhances the rough eye phenotype of CycEJP homozygotes.

Xenogenetic Interactions
Complementation and Rescue Data

The early larval lethality of E2f1rM729/Df(3R)Exel6186 transheterozygotes is partially rescues by Scer\GAL4Ubi.PU-driven expression of either E2f1UAS.a.Tag:FLAG,Tag:MYC or E2f1UAS.b.Tag:FLAG,Tag:MYC (giving rise to few, severely developmentally delayed third instar larvae and pupae, but no pharate adults), or of both transgenes (giving rise to the expected numbers of mildly developmentally delayed third instar larvae, pupae and to pharate adults, but to no adults).

Images (0)
Stocks (2)
Notes on Origin

G. Rubin.


Transposase-mediated excision of the P{PZ} produces ry- flies that lack both the insertion and the lethal phenotype.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (20)
References (33)