FlyBase Allele Report: Dmel\E2f1[rM729]

(Sainz de la Maza et al., 2022, Zappia et al., 2021, Steele et al., 2009, Reis and Edgar, 2004, Neufeld et al., 1998, Sawado et al., 1998, Brook et al., 1996, Duronio et al., 1995, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell adhesion | somatic clone | third instar larval stage

(Burguete et al., 2019)

abnormal cell shape | somatic clone | third instar larval stage

(Burguete et al., 2019)

abnormal developmental rate (with E2f1⁹¹)

(Frolov et al., 2001)

abnormal developmental rate | larval stage (with E2f1⁹¹)

(Du, 2000)

abnormal endomitotic cell cycle

(Duronio et al., 1995)

abnormal size | larval stage (with E2f1⁹¹)

(Du, 2000)

abnormal size | somatic clone | third instar larval stage

(Burguete et al., 2019)

decreased cell size | somatic clone | third instar larval stage

(Burguete et al., 2019)

decreased occurrence of cell division | larval stage (with E2f1⁹¹)

(Du, 2000)

increased cell size | somatic clone

(Neufeld et al., 1998)

lethal | recessive

(Du, 2000, Duronio et al., 1995)

lethal - all die before end of larval stage (with E2f1⁹¹)

(Frolov et al., 2001)

lethal - all die before end of second instar larval stage (with Df(3R)Exel6186)

(Kim et al., 2018)

some die during larval stage (with E2f1⁹¹)

(Frolov et al., 2001)

Phenotype Manifest In

adherens junction | somatic clone | third instar larval stage

(Burguete et al., 2019)

central nervous system

(Duronio et al., 1995)

embryonic/larval optic lobe

(Du, 2000)

embryonic/larval optic lobe | larval stage (with E2f1⁹¹)

(Du, 2000)

embryonic/larval tracheal stalk cell | somatic clone | third instar larval stage

(Burguete et al., 2019)

eye

(Brook et al., 1996)

eye-antennal disc

(Brook et al., 1996)

eye disc

(Du, 2000)

eye disc & mitotic cell cycle

(Du, 2000)

eye disc | larval stage (with E2f1⁹¹)

(Du, 2000)

eye disc | somatic clone

(Ambrus et al., 2007, Baonza and Freeman, 2005)

imaginal disc (with E2f1⁹¹)

(Frolov et al., 2001)

larval optic anlage | somatic clone

(Zhou and Luo, 2013)

neuroblast

(Duronio et al., 1995)

nucleus | somatic clone | third instar larval stage

(Burguete et al., 2019)

ommatidium

(Brook et al., 1996)

photoreceptor neuron

(Brook et al., 1996)

rhabdomere

(Brook et al., 1996)

terminal tracheal cell | somatic clone | third instar larval stage

(Burguete et al., 2019)

wing disc | somatic clone

(Neufeld et al., 1998)

Detailed Description

Statement

Reference

E2f1^rM729/E2f1^rM729 somatic clones of terminal tracheal cells are smaller in size, have reduced nuclei, less branches and show gas filling defects compared to wild-type controls in third instar larvae. Heterozygous neighboring stalk cells invade the small mutant terminal cells leading to the formation of two or more adherens junctions (not one as in wild-type controls ) between them and show a compensatory increase in cell size.

(Burguete et al., 2019)

Homozygous clones in the developing optic lobe are very small. Some of the mutant cells are extruded from the neuroepithelium and basally enter the medulla cortex, where ectopic neuroblasts form prematurely.

(Zhou and Luo, 2013)

E2f1^rM729 germline stem cell (GSC) clones behave similarly to control GSC clones and do not dramatically affect GSC maintenance.

(Jin et al., 2008)

Homozygous clones in the eye disc are very small.

(Ambrus et al., 2007)

E2f^rM729 eye disc clones in a Minute/+ background are extremely small.

(Baonza and Freeman, 2005)

E2f⁹¹/E2f^rM729 transheterozygous larvae, are much smaller than wild-type, 5 days after egg laying. They fail to develop into 3rd instar larvae.

(Frolov et al., 2001)

E2f⁹¹/E2f^rM729 transheterozygotes can develop into the late second instar or early third instar larval stage at a very slow pace (around 13 days after egg laying), but do not develop into pupae. BrdU incorporation is not seen in the optic lobe region of the brain in day 13 E2f⁹¹/E2f^rM729 larvae, in contrast to wild-type third instar larvae. The eye discs of E2f⁹¹/E2f^rM729 larvae are very small and have only a few cells labelled with BrdU with no specific pattern.

(Du, 2000)

Cells in homozygous clones induced in the wing pouch of the wing disc become enlarged and are gradually lost from the disc epithelium.

(Neufeld et al., 1998)

Clones induced in the eye in the first or second larval instar are not detected in the adult eye. They can be detected in the eye disc, but are much smaller than the adjacent twin spots, with fewer cells. These cells have initiated neuronal differentiation. Resulting ommatidia have incomplete sets of photoreceptors, and photoreceptors that are present often have abnormal morphology. Rhabdomeres are small in diameter, the rhabdomeres of the outer photoreceptors may project centrally and the photoreceptors do not extend the full length of the retina.

(Brook et al., 1996)

Cells in the CNS that normally enter endocycles fail at S₁₇. Replication either fails or occurs at a very low rate.

(Duronio et al., 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

E2f1⁹¹/E2f1^rM729 has abnormal developmental rate phenotype, suppressible by Df(2R)vg-B/Dp^a2

(Frolov et al., 2001)

E2f1⁹¹/E2f1^rM729 has abnormal developmental rate phenotype, suppressible by Df(2L)G5.1/E2f2^76Q1

(Frolov et al., 2001)

E2f1⁹¹/E2f1^rM729 has lethal phenotype, suppressible by Rbf¹⁴/Rbf^120a

(Du, 2000)

Enhancer of

Statement

Reference

Rbf^120a/E2f1^rM729 is an enhancer of increased cell death | somatic clone phenotype of Tsc1^f01910

(Hsieh et al., 2010)

E2f1^rM729 is an enhancer of visible | recessive phenotype of CycE^JP

(Secombe et al., 1998)

NOT Enhancer of

Statement

Reference

E2f1^rM729 is a non-enhancer of visible phenotype of Dp^GMR.PD, E2f1^GMR.PD

(Staehling-Hampton et al., 1999)

Suppressor of

Statement

Reference

E2f1^rM729/E2f1^rM729 is a suppressor of abnormal cell number | somatic clone | spermatogenesis phenotype of Rbf^GL01293, Scer\GAL4^Tub.PU

(Sainz de la Maza et al., 2022)

E2f1ⁱ²/E2f1^rM729 is a suppressor of increased cell death | somatic clone | third instar larval stage phenotype of Rbf^15aΔ, Stam¹⁹

(Sheng et al., 2016)

E2f1^rM729 is a suppressor of increased cell death | somatic clone phenotype of Rbf^120a, Tsc1^f01910

(Hsieh et al., 2010)

E2f[+]/E2f1^rM729 is a suppressor of increased cell death phenotype of Dref^UAS.cSa, Scer\GAL4^GMR.PS

(Hirose et al., 2001)

NOT Suppressor of

Statement

Reference

E2f1^rM729 is a non-suppressor of visible phenotype of Dp^GMR.PD, E2f1^GMR.PD

(Staehling-Hampton et al., 1999)

Other

Statement

Reference

E2f1⁹¹/E2f1^rM729, E2f2^g5/E2f2^76Q1 has decreased cell death | wandering third instar larval stage phenotype

(Moon et al., 2005)

E2f1ⁱ²/E2f1^rM729, Rbf¹⁴ has partially lethal phenotype

(Du, 2000)

Phenotype Manifest In

Suppressed by

Statement

Reference

E2f1^rM729 has eye | somatic clone phenotype, suppressible by l(3)mbt^6a14

(Ambrus et al., 2009)

E2f1^rM729 has eye | somatic clone phenotype, suppressible by l(3)mbt^4d30

(Ambrus et al., 2009)

E2f1^rM729 has eye | somatic clone phenotype, suppressible by l(3)mbt^3d23

(Ambrus et al., 2009)

E2f1^rM729 has eye | somatic clone phenotype, suppressible by l(3)mbt^5d8

(Ambrus et al., 2009)

E2f1^rM729 has eye | somatic clone phenotype, suppressible by l(3)mbt^4d16

(Ambrus et al., 2009)

E2f1^rM729 has eye | somatic clone phenotype, suppressible by l(3)mbt^5d6

(Ambrus et al., 2009)

E2f1^rM729 has eye | somatic clone phenotype, suppressible by l(3)mbt^6a31

(Ambrus et al., 2009)

E2f1^rM729 has eye | somatic clone phenotype, suppressible by l(3)mbt^3d33

(Ambrus et al., 2009)

E2f1^rM729 has eye | somatic clone phenotype, suppressible by Doa^6d2

(Ambrus et al., 2009)

E2f1^rM729 has eye | somatic clone phenotype, suppressible by Doa^6a21

(Ambrus et al., 2009)

E2f1^rM729 has eye | somatic clone phenotype, suppressible by Doa^5d19

(Ambrus et al., 2009)

E2f1^rM729 has eye | somatic clone phenotype, suppressible by Doa^6a52

(Ambrus et al., 2009)

E2f1^rM729 has eye | somatic clone phenotype, suppressible by Doa^5d7

(Ambrus et al., 2009)

E2f1^rM729 has eye | somatic clone phenotype, suppressible by gfzf^6a27

(Ambrus et al., 2009)

E2f1^rM729 has eye | somatic clone phenotype, suppressible by B52^5d21

(Ambrus et al., 2009)

E2f1^rM729 has eye | somatic clone phenotype, suppressible by B52^3d16

(Ambrus et al., 2009)

E2f1^rM729 has eye | somatic clone phenotype, suppressible by B52^3d24

(Ambrus et al., 2009)

E2f1^rM729 has eye | somatic clone phenotype, suppressible by Slimp^7d21

(Ambrus et al., 2009)

E2f1^rM729 has eye | somatic clone phenotype, suppressible by Slimp^f07858

(Ambrus et al., 2009)

E2f1^rM729 has eye | somatic clone phenotype, suppressible by bel^7d19

(Ambrus et al., 2009)

E2f1^rM729 has eye | somatic clone phenotype, suppressible by Su(E1)-A^7d13

(Ambrus et al., 2009)

E2f1^rM729 has eye | somatic clone phenotype, suppressible by Su(E1)-A^6a39

(Ambrus et al., 2009)

E2f1^rM729 has eye | somatic clone phenotype, suppressible by Su(E1)-B^4d26

(Ambrus et al., 2009)

E2f1^rM729 has eye | somatic clone phenotype, suppressible by Su(E1)-B^6a11

(Ambrus et al., 2009)

E2f1^rM729 has eye | somatic clone phenotype, suppressible by Su(E1)-C^1d24

(Ambrus et al., 2009)

E2f1^rM729 has eye | somatic clone phenotype, suppressible by Su(E1)-C^3d28

(Ambrus et al., 2009)

E2f1^rM729 has eye | somatic clone phenotype, suppressible by Su(E1)-D^6a47

(Ambrus et al., 2009)

E2f1^rM729 has eye | somatic clone phenotype, suppressible by Su(E1)-E^3d25

(Ambrus et al., 2009)

E2f1^rM729 has eye | somatic clone phenotype, suppressible by Su(E1)-F^6b9

(Ambrus et al., 2009)

E2f1^rM729 has eye | somatic clone phenotype, suppressible by Su(E1)-G^6a3

(Ambrus et al., 2009)

E2f1^rM729 has eye | somatic clone phenotype, suppressible by Su(E1)-H^6a50

(Ambrus et al., 2009)

E2f1^rM729 has eye | somatic clone phenotype, suppressible by Su(E1)-I^6d9

(Ambrus et al., 2009)

E2f1^rM729 has eye | somatic clone phenotype, suppressible by Su(E1)-J^6a8

(Ambrus et al., 2009)

E2f1^rM729 has eye | somatic clone phenotype, suppressible by Su(E1)-K^6b12

(Ambrus et al., 2009)

E2f1^rM729 has eye | somatic clone phenotype, suppressible by Su(E1)-L^6b19

(Ambrus et al., 2009)

E2f1^rM729 has eye | somatic clone phenotype, suppressible by Su(E1)-M^6b31

(Ambrus et al., 2009)

E2f1^rM729 has eye | somatic clone phenotype, suppressible by Su(E1)-N^4d22

(Ambrus et al., 2009)

E2f1^rM729 has eye | somatic clone phenotype, suppressible by Su(E1)-O^6a19

(Ambrus et al., 2009)

E2f1^rM729 has eye disc | somatic clone phenotype, suppressible by E2f2^c03344

(Ambrus et al., 2007)

E2f1^rM729 has eye disc | somatic clone phenotype, suppressible by bel^7d19

(Ambrus et al., 2007)

E2f1^rM729 has eye disc | somatic clone phenotype, suppressible by bel^L4740

(Ambrus et al., 2007)

E2f1^rM729 has eye disc | somatic clone phenotype, suppressible by smo¹

(Ambrus et al., 2007)

E2f1⁹¹/E2f1^rM729 has imaginal disc phenotype, suppressible by Df(2L)G5.1/E2f2^76Q1

(Frolov et al., 2001)

E2f1⁹¹/E2f1^rM729 has embryonic/larval optic lobe phenotype, suppressible by Rbf¹⁴/Rbf^120a

(Du, 2000)

E2f1⁹¹/E2f1^rM729 has eye disc phenotype, suppressible by Rbf¹⁴/Rbf^120a

(Du, 2000)

E2f1⁹¹/E2f1^rM729 has eye disc & mitotic cell cycle phenotype, suppressible by Rbf¹⁴/Rbf^120a

(Du, 2000)

NOT suppressed by

Statement

Reference

E2f1^rM729 has eye disc | somatic clone phenotype, non-suppressible by Su(H)^VP16.UAS/Scer\GAL4^GMR.PF

(Baonza and Freeman, 2005)

Enhancer of

Statement

Reference

E2f1^rM729 is an enhancer of eye disc | somatic clone phenotype of Tsc1^f01910

(Hsieh et al., 2010)

Rbf^120a/E2f1^rM729 is an enhancer of eye disc | somatic clone phenotype of Tsc1^f01910

(Hsieh et al., 2010)

E2f1^rM729, Scer\GAL4^GMR.PF is an enhancer of eye phenotype of E2f2^UAS.cFa, Scer\GAL4^GMR.PF

(Frolov et al., 2001)

E2f1^rM729, Scer\GAL4^GMR.PF is an enhancer of ommatidium phenotype of E2f2^UAS.cFa, Scer\GAL4^GMR.PF

(Frolov et al., 2001)

E2f1^rM729 is an enhancer of eye phenotype of CycE^JP

(Secombe et al., 1998)

Suppressor of

Statement

Reference

E2f1^rM729/E2f1^rM729 is a suppressor of somatic cell of testis | somatic clone phenotype of Rbf^GL01293, Scer\GAL4^Tub.PU

(Sainz de la Maza et al., 2022)

E2f1ⁱ²/E2f1^rM729 is a suppressor of eye disc | somatic clone | third instar larval stage phenotype of Rbf^15aΔ, Stam¹⁹

(Sheng et al., 2016)

E2f1^rM729 is a suppressor of eye disc | somatic clone phenotype of Rbf^120a, Tsc1^f01910

(Hsieh et al., 2010)

E2f1^rM729 is a suppressor of eye disc morphogenetic furrow | somatic clone phenotype of Rbf^120a, Tsc1^f01910

(Hsieh et al., 2010)

E2f1ⁱ²/E2f1^rM729 is a suppressor of eye | somatic clone phenotype of Rbf^15aΔ, rno¹

(Steele et al., 2009)

E2f1ⁱ²/E2f1^rM729 is a suppressor of photoreceptor cell R8 | somatic clone | third instar larval stage phenotype of Rbf^15aΔ, rno¹

(Steele et al., 2009)

E2f1^rM729 is a suppressor of female germline stem cell | germline clone phenotype of bam^Δ86

(Jin et al., 2008)

E2f[+]/E2f1^rM729 is a suppressor of eye phenotype of Dref^UAS.cSa, Scer\GAL4^GMR.PS

(Hirose et al., 2001)

Other

Statement

Reference

E2f1⁹¹/E2f1^rM729, E2f2^c03344/E2f2^76Q1 has larval fat cell of larval fat body | third instar larval stage phenotype

(Zappia et al., 2021)

E2f1⁹¹/E2f1^rM729, E2f2^c03344/E2f2^76Q1 has nucleus | increased number | third instar larval stage phenotype

(Zappia et al., 2021)

E2f1⁹¹/E2f1^rM729, E2f2^g5/E2f2^76Q1 has wing disc | wandering third instar larval stage phenotype

(Moon et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

E2f2^76Q1/E2f2^c03344, E2f1⁹¹/E2f1^rM729 third instar larvae exhibit binucleated cells in the fat body.

(Zappia et al., 2021)

The high levels of apoptosis (detected by Caspase-3 staining) observed in Rbf^15aΔ;Stam¹⁹ double homozygous somatic clones in third instar larval eye disc is completely abolished when the clones are created in E2f1[i2]/E2f1[rM729] mutant background.

(Sheng et al., 2016)

An E2f1^rM729 mutant background dominantly enhances the Scer\GAL4^GMR.PF>lin-52^GD12885 eye phenotype. This is accompanied by a corresponding increase in hid protein and elevated apoptosis in the eye-antennal disc.

(Bhaskar et al., 2014)

Tsc1^f01910 E2f^rM729 double mutant eye disc clones in a wild-type background are smaller than Tsc1^f01910 single mutant eye disc clones.

When Tsc1^f01910 E2f^rM729 double mutant clones are generated in Rbf^120a, the sizes of the Tsc1^f01910 E2f^rM729 double mutant clones are much smaller than that of Tsc1^f01910 single mutant clones.

The prevailing cell death phenotype observed in Rbf^120a Tsc1^f01910 double mutant clones at the morphogenetic furrow is no longer present in Rbf^120a E2f^rM729 Tsc1^f01910 triple mutant cells, indicating that the increased level of ectopic cell death observed in Rbf^120a Tsc1^f01910 double mutant cells is E2f-dependent.

(Hsieh et al., 2010)

Each of l(3)mbt^6a14, l(3)mbt^4d30, l(3)mbt^3d23, l(3)mbt^5d8, l(3)mbt^4d16, l(3)mbt^5d6, l(3)mbt^6a31, l(3)mbt^3d33, Doa^6d2, Doa^6a21, Doa^5d7, gfzf^6a27, B52^5d21, B52^3d16, B52^3d24, CG31133^7d21, CG31133^f07858, bel^7d19, Su(E1)-A^7d13, Su(E1)-A^6a39, Su(E1)-B^4d26, Su(E1)-B^6a11, Su(E1)-C^1d24, Su(E1)-C^3d28, Su(E1)-D^6a47, Su(E1)-E^3d25, Su(E1)-F^6b9, Su(E1)-G^6a3, Su(E1)-H^6a50, Su(E1)-I^6d9, Su(E1)-J^6a8, Su(E1)-K^6b12, Su(E1)-L^6b19, Su(E1)-M^6b31, Su(E1)-N^4d22 and Su(E1)-O^6a19 can suppress the proliferation block of E2f^rM729 clones, allowing recovery of double mutant clones in the eye.

The second mitotic wave is strongly restored in l(3)mbt^3d33 E2f^rM729 and Su(E1)-A^7d13 E2f^rM729 double mutant clones in the eye disc.

The second mitotic wave is weakly rescued in CG31133^7d21 E2f^rM729 and gfzf^6a27 E2f^rM729 double mutant clones in the eye disc, with the double mutant cells entering the second mitotic wave several columns more posterior than in wild-type cells. In addition BrdU incorporation persists several columns more posterior in the double mutant cells than in the adjacent wild-type cells.

The second mitotic wave is weakly rescued in Doa^6d2 E2f^rM729 double mutant clones in the eye disc, with double mutant cells only being sporadically labelled with BrdU in the second mitotic wave. Those double mutant cells that do incorporate BrdU are always found to be significantly more than adjacent wild-type cells that incorporate BrdU.

(Ambrus et al., 2009)

The shiny-eye and multiple photoreceptor-R8 phenotype seen in rno¹, Rbf^15aΔ eye somatic mutant clones is significantly suppressed in a E2fⁱ²/E2f^rM729 mutant background.

(Steele et al., 2009)

bam^Δ86 E2f1^rM729 double mutant germline stem cell (GSC) clones are much less competitive than bam^Δ86 single mutant GSCs.

(Jin et al., 2008)

Relatively large E2f2^c03344 E2f^rM729 double mutant clones can be recovered in the eye disc.

The small size of E2f^rM729 clones in the eye disc is partially rescued if they are also mutant for either bel^7d19, bel^L4740 or smo¹.

(Ambrus et al., 2007)

Scer\GAL4^GMR.PF-driven expression of Su(H)^{Scer\UAS.T:Hsim\VP16} is unable to rescue the small size of E2f^rM729 eye disc clones.

(Baonza and Freeman, 2005)

In wild-type animals, exposure to 40Gy of γ rays induces a distinct pattern of apoptosis in the wing disc, focussed on the the wing pouch, but excluding the DV boundary region and prospective vein. In E2f^rM729/E2f⁹¹; E2f2^76Q1/E2f2^g5 animals, the same irradiation treatment induces a different pattern of apoptosis a smaller number of apoptotic cells are clustered in the center of the wing pouch, many in the D-V boundary region or prospective veins.

(Moon et al., 2005)

The addition of E2f2^76Q1/Df(2L)G5.1 to E2f⁹¹/E2f^rM729 flies almost completely suppresses the E2f⁹¹/E2f^rM729 phenotypes - mutants develop normally without any significant delay in larval growth, reach pupal stage and finally die as mid- or late pupae. The pattern of DNA synthesis in eye discs is largely normal. The addition of Dp^a2/Df(2R)vg-B to E2f⁹¹/E2f^rM729 flies almost completely suppresses the E2f⁹¹/E2f^rM729 phenotypes - mutants develop normally without any significant delay in larval growth.

(Frolov et al., 2001)

The rough eye phenotype seen upon expression of Dref^Scer\UAS.cSa in the developing eye under the control of Scer\GAL4^GMR.PS is suppressed in an E2f^rM729 heterozygous background.

(Hirose et al., 2001)

Approximately 50% of the expected number of Rbf¹⁴/Y ; E2fⁱ²/E2f^rM729 flies survive to the adult stage, and the development of these flies is not significantly delayed compared to sibling flies. The surviving flies have normal eyes, normal macrochaetae on the notum and normal wings and legs. The larval lethality of E2f⁹¹/E2f^rM729 animals can be suppressed by Rbf^120a/Rbf¹⁴; the larvae develop into large late third instar larvae and many can pupate. Some Rbf^120a/Rbf¹⁴ ; E2f⁹¹/E2f^rM729 animals can develop into pharate adults with adult eyes, legs and wing structures. The development of these larvae is significantly retarded compared to wild-type larvae, with the earliest pupae observed at around day 11 after egg laying. The normal pattern of DNA replication in the optic lobe region is restored in E2f⁹¹/E2f^rM729 larvae that are also carrying Rbf^120a/Rbf¹⁴. In Rbf^120a/Rbf¹⁴ ; E2f⁹¹/E2f^rM729 eye discs the overall pattern of DNA replication is normal. No significant cell death is seen in these eye discs. In late third instar discs there are about 25 rows of developing photoreceptor cells (as in wild-type discs) but there are much fewer cells anterior to the morphogenetic furrow than in wild type.

(Du, 2000)

Dominantly enhances the rough eye phenotype of CycE^JP homozygotes.

(Secombe et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

E2f1⁰⁷¹⁷²

(BDGP Project Members, 1994-1999)

Partially rescued by

Df(3R)Exel6186/E2f1^rM729 is partially rescued by E2f1^{UAS.a.Tag:FLAG,Tag:MYC}/Scer\GAL4^Ubi.PU

(Kim et al., 2018)

Df(3R)Exel6186/E2f1^rM729 is partially rescued by E2f1^{UAS.a.Tag:FLAG,Tag:MYC}/Scer\GAL4^Ubi.PU/E2f1^{UAS.b.Tag:FLAG,Tag:MYC}

(Kim et al., 2018)

Df(3R)Exel6186/E2f1^rM729 is partially rescued by Scer\GAL4^Ubi.PU/E2f1^{UAS.b.Tag:FLAG,Tag:MYC}

(Kim et al., 2018)

Comments

The early larval lethality of E2f1^rM729/Df(3R)Exel6186 transheterozygotes is partially rescues by Scer\GAL4^Ubi.PU-driven expression of either E2f1^{UAS.a.Tag:FLAG,Tag:MYC} or E2f1^{UAS.b.Tag:FLAG,Tag:MYC} (giving rise to few, severely developmentally delayed third instar larvae and pupae, but no pharate adults), or of both transgenes (giving rise to the expected numbers of mildly developmentally delayed third instar larvae, pupae and to pharate adults, but to no adults).

(Kim et al., 2018)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

34054

ry⁵⁰⁶ P{PZ}E2f1^rM729/TM3, Sb¹

35849

w^*; P{neoFRT}82B P{PZ}E2f1^rM729/TM3, Sb¹

Notes on Origin

Discoverer

G. Rubin.

Comments

Complements: InR⁰⁵⁵⁴⁵.

(BDGP Project Members, 1994-1999)

Transposase-mediated excision of the P{PZ} produces ry^- flies that lack both the insertion and the lethal phenotype.

(Duronio et al., 1995)

mir-11^Δ1 complements E2f^rM729.

(Truscott et al., 2011)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (21)

Reported As

Symbol Synonym

E2F1⁷²⁹

(Zhou and Luo, 2013)

E2F^rM729

(McPherson et al., 2023, Burguete et al., 2019)

E2F^rm729

(Heriche et al., 2003, Hwang et al., 2002, Asano and Wharton, 1999, Staehling-Hampton et al., 1999)

E2f1⁷²⁹

(Herrera et al., 2021)

E2f1^m729

(Zappia et al., 2021)

E2f1^rM729

(Sainz de la Maza et al., 2022)

E2f1^rm729

(Bhaskar et al., 2014)

E2f⁷²⁹

(Hirose et al., 2001, Brook et al., 1996)

E2f^rM729

E2f^rm729

(Li et al., 2010)

dE2F1⁷²⁹

(Reis and Edgar, 2004)

dE2F1^rM729

(Jin et al., 2008, Baonza and Freeman, 2005)

dE2F⁷²⁹

(Hirose et al., 2001, Neufeld et al., 1998, Sawado et al., 1998)

dE2F^RM729

(Secombe et al., 1998)

dE2f1^rM729

(Truscott et al., 2011)

dE2f1^rm729

(Steele et al., 2009)

de2f1⁷²⁹

(Kim et al., 2018, Hsieh et al., 2010, Ambrus et al., 2009, Ambrus et al., 2007, Moon et al., 2005)

de2f1^rm729

(Kim et al., 2018, Sheng et al., 2016, Frolov et al., 2003, Frolov et al., 2001, Du, 2000)

e2f1^rM729

(Zhang et al., 2021)

e2f1^rm729

(Wang et al., 2020)

l(3)rM729^rM729

Name Synonyms

Secondary FlyBase IDs

References (36)

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