FlyBase Allele Report: Dmel\mys[8]

General Information

Symbol

Dmel\mys⁸

Species

D. melanogaster

Name

FlyBase ID

FBal0012685

Feature type

allele

Associated gene

Dmel\mys

Associated Insertion(s)

Carried in Construct

Also Known As

mys^nj42, mys^nj, nj⁴²

Key Links

Allele class

heat sensitive loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

heat sensitive loss of function allele

(Bunch et al., 1992)

Mutagen

ethyl methanesulfonate

(de la Pompa et al., 1989, Digan et al., 1986)

Progenitor genotype

Cytology

Description

May contain regulatory mutation as lesion not found within exons or splice sites of mys.

(Jannuzi et al., 2004)

Indistinguishable from wild type on Southern blot. Immunoblots indicate β_PS protein levels are comparable to wild type (within a factor of two).

(Bunch et al., 1992)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

10% of mutant males show wing blisters.

(Fraichard et al., 2010)

Hub cells fail to localise to the apical tip of the testis in homozygous males.

(Lee et al., 2008)

Avoidance of benzaldehyde is blunted in mutant animals compared to wild type.

mys^ts1/mys⁸ and mys^ts2/mys⁸ flies show normal odour avoidance behaviour.

(Bhandari et al., 2006)

0.3% of hemizygous mutant wings show a wing blister phenotype.

(Schock and Perrimon, 2003)

mys⁸ larvae show a reduced olfactory response to isoamyl acetate and n-butanol, but are unaffected in their response to ethyl acetate and benzaldehyde. mys⁸/mys^{olfC-A459.1F1} flies survive and show no defect in jumping ability. They show a reduced olfactory response to isoamyl acetate but respond normally to ethyl acetate.

(Ayyub et al., 2000)

Some venation abnormalities are seen in homozygous mutant adults. mys⁸/Y flies have a low frequency of wing blisters (=<10%).

(D'Avino and Thummel, 2000)

2% of hemizygous males have wing blisters.

(Walsh and Brown, 1998)

Wing blisters are seen in approximately 5-10% of hemizygotes, and approximately 30-50% of hemizygotes have held-out wings.

(Prout et al., 1997)

Retinal development begins to break down at 55% of pupal life: one half of the R8's cell bodies have fallen though the retinal floor coming to lie underneath the cone cell plate, interrupting cone cell plate contacts. By 67% of pupal life the pigment cell feet have pulled away from the grommet extracellular membrane, opening holes around the cone cell plate. By the adult stage the retinal floor is completely disrupted. Rhabdomeres buckle. Temperature shift experiments reveal that the mys function is required during cone cell plate formation.

(Longley and Ready, 1995)

Wings are held out at an angle of 90^o to the body, when flies are reared at 29^oC.

(Grinblat et al., 1994)

mys^xR04/mys⁸ flies die at 25^oC but survive at 18^oC, and have inflated or blistered wings and often show a gap between the pigment cell feet constituting the fenestrated membrane and the layer of pigmented subretinal cells found below the basement membrane. The viable mys⁸ adults have held-out wings, a phenotype which is enhanced at 29^oC. mys⁸ males have blistered wings.

(Zusman et al., 1993)

Homozygotes can survive at 18^oC, but their number decreases gradually as the rearing temperature is raised to 28^oC where none survive.

(Bunch et al., 1992)

Homozygous wing is wild type in appearance but sometimes held abnormally away from the body due to severe wasting of the mesothoracic and metathoracic tergotrochanteral muscles. This phenotype is enhanced in heterozygotes. In combination with Df(1)C128 wings display morphogenetic defects, wing blisters, the formation of extra crossveins between L2 and L3 and abnormal 'delta' thickenings at vein intersections. Heterozygotes with mys^xR04 are lethal. Double hemizygous and homozygous mutants with mys^xR04 are lethal.

(Wilcox et al., 1989)

Homozygotes show no jumping response and severe muscular defects.

(de la Pompa et al., 1989)

adults survive as nonjumpers

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

mys⁸ has visible phenotype, enhanceable by tnc^RNAi.UAS.cFa/Scer\GAL4^Act5C.PI

(Fraichard et al., 2010)

mys⁸ has visible | recessive phenotype, enhanceable by bs^V100/bs[+]

(Walsh and Brown, 1998)

mys⁸ has visible | recessive phenotype, enhanceable by puri[+]/puri^V4

(Walsh and Brown, 1998)

mys⁸ has visible | recessive phenotype, enhanceable by moa[+]/jbug^V50

(Walsh and Brown, 1998)

mys⁸ has visible | recessive phenotype, enhanceable by auk^2R-4/auk[+]

(Prout et al., 1997)

mys⁸ has visible | recessive phenotype, enhanceable by stck[+]/stck^3R-17

(Prout et al., 1997)

mys⁸ has visible | recessive phenotype, enhanceable by bub^2L-2/bub[+]

(Prout et al., 1997)

mys⁸ has visible | recessive phenotype, enhanceable by 2R-F[+]/2R-F^2R-22

(Prout et al., 1997)

mys⁸ has visible | recessive phenotype, enhanceable by 2R-L[+]/2R-L^84-2

(Prout et al., 1997)

mys⁸ has visible | recessive phenotype, enhanceable by auk^unspecified/auk[+]

(Prout et al., 1997)

mys⁸ has visible | recessive phenotype, enhanceable by shot^65-2/shot[+]

(Prout et al., 1997)

mys⁸ has visible | recessive phenotype, enhanceable by bs[+]/bs^2R-19

(Prout et al., 1997)

mys⁸ has visible | recessive phenotype, enhanceable by bs[+]/bs^unspecified

(Prout et al., 1997)

mys⁸ has visible | recessive phenotype, enhanceable by shot^unspecified/shot[+]

(Prout et al., 1997)

mys⁸ has visible | recessive phenotype, enhanceable by blo[+]/blo^unspecified

(Prout et al., 1997)

mys⁸ has visible | recessive phenotype, enhanceable by kiwi^unspecified/kiwi[+]

(Prout et al., 1997)

mys⁸ has visible | recessive phenotype, enhanceable by mam^unspecified/mam[+]

(Prout et al., 1997)

mys⁸ has visible | recessive phenotype, enhanceable by pio[+]/pio^unspecified

(Prout et al., 1997)

mys⁸ has visible | recessive phenotype, enhanceable by rhea[+]/rhea¹

(Prout et al., 1997)

mys⁸ has visible | recessive phenotype, enhanceable by rhea[+]/rhea²

(Prout et al., 1997)

mys⁸ has visible | recessive phenotype, enhanceable by stck[+]/stck^unspecified

(Prout et al., 1997)

mys⁸ has visible | recessive phenotype, enhanceable by blo^2R-17/blo[+]

(Prout et al., 1997)

mys⁸ has visible | recessive phenotype, enhanceable by kiwi^2R-3/kiwi[+]

(Prout et al., 1997)

mys⁸ has visible | recessive phenotype, enhanceable by pio[+]/pio^2R-16

(Prout et al., 1997)

mys⁸/mys^G has visible phenotype, enhanceable by bs[+]/bs^unspecified

(Wessendorf et al., 1992)

Enhancer of

Statement

Reference

mys⁸/mys[+] is an enhancer of visible | female phenotype of Fak^UAS.Tag:HA, Scer\GAL4^en-e16E

(Grabbe et al., 2004)

mys⁸/mys[+] is an enhancer of visible | dominant phenotype of sog^EP7

(Araujo et al., 2003)

mys⁸/mys⁸ is an enhancer of visible | dominant phenotype of sog^EP7

(Araujo et al., 2003)

mys⁸/mys[+] is an enhancer of visible | dominant phenotype of sog^EP11

(Araujo et al., 2003)

mys⁸/mys⁸ is an enhancer of visible | dominant phenotype of sog^EP11

(Araujo et al., 2003)

Suppressor of

Statement

Reference

mys⁸ is a suppressor | partially of lethal phenotype of Scer\GAL4^Act5C.PI, tnc^EY16369

(Fraichard et al., 2010)

Other

Statement

Reference

Fak^UAS.Tag:HA, Scer\GAL4^en-e16E, mys⁸/mys[+] has visible | male phenotype

(Grabbe et al., 2004)

Fak^UAS.Tag:HA, Scer\GAL4^en-e16E, mys⁸ has visible | male phenotype

(Grabbe et al., 2004)

cass^E48/cass[+], mys⁸ has visible | recessive phenotype

(Walsh and Brown, 1998)

cno^mis1, mys⁸ has partially lethal phenotype

(Miyamoto et al., 1995)

Phenotype Manifest In

Enhanced by

Statement

Reference

mys⁸ has wing phenotype, enhanceable by tnc^RNAi.UAS.cFa/Scer\GAL4^Act5C.PI

(Fraichard et al., 2010)

mys⁸ has hub cell phenotype, enhanceable by Lasp[+]/Lasp¹

(Lee et al., 2008)

mys⁸ has wing phenotype, enhanceable by wb[+]/wb^4Y18

(Schock and Perrimon, 2003)

mys⁸ has wing phenotype, enhanceable by crol^k08217

(D'Avino and Thummel, 2000)

mys⁸ has wing phenotype, enhanceable by EcR^C300Y

(D'Avino and Thummel, 2000)

mys⁸ has wing phenotype, enhanceable by EcR^M554fs

(D'Avino and Thummel, 2000)

mys⁸ has wing phenotype, enhanceable by crol^3ex5

(D'Avino and Thummel, 2000)

mys⁸ has wing phenotype, enhanceable by bs^V100/bs[+]

(Walsh and Brown, 1998)

mys⁸ has wing phenotype, enhanceable by puri[+]/puri^V4

(Walsh and Brown, 1998)

mys⁸ has wing phenotype, enhanceable by moa[+]/jbug^V50

(Walsh and Brown, 1998)

mys⁸ has wing phenotype, enhanceable by auk^2R-4/auk[+]

(Prout et al., 1997)

mys⁸ has wing phenotype, enhanceable by stck[+]/stck^3R-17

(Prout et al., 1997)

mys⁸ has wing phenotype, enhanceable by bub^2L-2/bub[+]

(Prout et al., 1997)

mys⁸ has wing phenotype, enhanceable by 2R-F[+]/2R-F^2R-22

(Prout et al., 1997)

mys⁸ has wing phenotype, enhanceable by 2R-L[+]/2R-L^84-2

(Prout et al., 1997)

mys⁸ has phenotype, enhanceable by Delta^unspecified

(Prout et al., 1997)

mys⁸ has wing phenotype, enhanceable by auk^unspecified/auk[+]

(Prout et al., 1997)

mys⁸ has wing phenotype, enhanceable by bs[+]/bs^2R-19

(Prout et al., 1997)

mys⁸ has wing phenotype, enhanceable by bs[+]/bs^unspecified

(Prout et al., 1997)

mys⁸ has wing phenotype, enhanceable by shot^unspecified/shot[+]

(Prout et al., 1997)

mys⁸ has wing phenotype, enhanceable by blo[+]/blo^unspecified

(Prout et al., 1997)

mys⁸ has wing phenotype, enhanceable by shot^65-2/shot[+]

(Prout et al., 1997)

mys⁸ has wing phenotype, enhanceable by kiwi^unspecified/kiwi[+]

(Prout et al., 1997)

mys⁸ has wing phenotype, enhanceable by mam^unspecified/mam[+]

(Prout et al., 1997)

mys⁸ has wing phenotype, enhanceable by pio[+]/pio^unspecified

(Prout et al., 1997)

mys⁸ has wing phenotype, enhanceable by rhea[+]/rhea¹

(Prout et al., 1997)

mys⁸ has wing phenotype, enhanceable by rhea[+]/rhea²

(Prout et al., 1997)

mys⁸ has wing phenotype, enhanceable by stck[+]/stck^unspecified

(Prout et al., 1997)

mys⁸ has wing phenotype, enhanceable by blo^2R-17/blo[+]

(Prout et al., 1997)

mys⁸ has wing phenotype, enhanceable by kiwi^2R-3/kiwi[+]

(Prout et al., 1997)

mys⁸ has wing phenotype, enhanceable by pio[+]/pio^2R-16

(Prout et al., 1997)

mys⁸/mys^G has wing phenotype, enhanceable by bs[+]/bs^unspecified

(Wessendorf et al., 1992)

mys⁸ has wing phenotype, enhanceable by if³

(Wilcox et al., 1989)

NOT Enhanced by

Statement

Reference

mys⁸ has wing phenotype, non-enhanceable by LanA^9-32/LanA[+]

(Schock and Perrimon, 2003)

mys⁸ has wing phenotype, non-enhanceable by crol⁰⁴⁴¹⁸

(D'Avino and Thummel, 2000)

mys⁸ has wing phenotype, non-enhanceable by crol⁰⁶⁴⁷⁰

(D'Avino and Thummel, 2000)

mys⁸ has wing phenotype, non-enhanceable by auk^V106/auk[+]

(Walsh and Brown, 1998)

mys⁸ has wing phenotype, non-enhanceable by auk[+]/auk^V162

(Walsh and Brown, 1998)

mys⁸ has wing phenotype, non-enhanceable by blo[+]/blo^V134

(Walsh and Brown, 1998)

mys⁸ has wing phenotype, non-enhanceable by blo^V99/blo[+]

(Walsh and Brown, 1998)

mys⁸ has wing phenotype, non-enhanceable by bs[+]/bs^V121

(Walsh and Brown, 1998)

mys⁸ has wing phenotype, non-enhanceable by cass^E44/cass[+]

(Walsh and Brown, 1998)

mys⁸ has wing phenotype, non-enhanceable by shot^V104/shot[+]

(Walsh and Brown, 1998)

mys⁸ has wing phenotype, non-enhanceable by shot[+]/shot^V167

(Walsh and Brown, 1998)

mys⁸ has wing phenotype, non-enhanceable by jbug^V88/moa[+]

(Walsh and Brown, 1998)

mys⁸ has wing phenotype, non-enhanceable by puri[+]/puri^V15

(Walsh and Brown, 1998)

mys⁸ has wing phenotype, non-enhanceable by pyg[+]/pyg^E16

(Walsh and Brown, 1998)

mys⁸ has wing phenotype, non-enhanceable by pyg^E50/pyg[+]

(Walsh and Brown, 1998)

mys⁸ has wing phenotype, non-enhanceable by how[+]/how^T1

(Walsh and Brown, 1998)

mys⁸ has wing phenotype, non-enhanceable by how[+]/how^T90

(Walsh and Brown, 1998)

mys⁸ has wing phenotype, non-enhanceable by tak[+]/tak^V27

(Walsh and Brown, 1998)

mys⁸ has wing phenotype, non-enhanceable by tak^V42/tak[+]

(Walsh and Brown, 1998)

mys¹¹/mys⁸ has wing phenotype, non-enhanceable by pot[+]/pot^P3

(Walsh and Brown, 1998)

Enhancer of

Statement

Reference

mys⁸/mys[+] is an enhancer of wing | female phenotype of Fak^UAS.Tag:HA, Scer\GAL4^en-e16E

(Grabbe et al., 2004)

mys⁸/mys[+] is an enhancer of wing vein phenotype of sog^EP7

(Araujo et al., 2003)

mys⁸/mys⁸ is an enhancer of wing vein phenotype of sog^EP7

(Araujo et al., 2003)

mys⁸/mys[+] is an enhancer of wing vein phenotype of sog^EP11

(Araujo et al., 2003)

mys⁸/mys⁸ is an enhancer of wing vein phenotype of sog^EP11

(Araujo et al., 2003)

mys⁸ is an enhancer of phenotype of bs^unspecified

(Fristrom et al., 1994)

mys⁸ is an enhancer of wing phenotype of if³

(Wilcox et al., 1989)

Other

Statement

Reference

Fak^UAS.Tag:HA, Scer\GAL4^en-e16E, mys⁸/mys[+] has wing | male phenotype

(Grabbe et al., 2004)

Fak^UAS.Tag:HA, Scer\GAL4^en-e16E, mys⁸ has wing | male phenotype

(Grabbe et al., 2004)

cass^E48/cass[+], mys⁸ has wing phenotype

(Walsh and Brown, 1998)

cno^mis1, mys⁸ has wing vein phenotype

(Miyamoto et al., 1995)

if³, mys⁸ has wing phenotype

(Brabant and Brower, 1993)

Additional Comments

Genetic Interactions

Statement

Reference

The frequency of wing blisters seen in mys⁸/Y flies is increased to 54% if they are also expressing tnc^{dsRNA.Scer\UAS.cFa} under the control of Scer\GAL4^Act5C.PI, and the overall frequency of wing defects is increased to 68%.

(Fraichard et al., 2010)

mys⁸ ; Lasp¹/+ males show a stronger hub cell mislocalisation phenotype than mys⁸ males. The double mutant males have wild-type fertility.

(Lee et al., 2008)

The penetrance of the wing blistering phenotype seen in females expressing Fak56D^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^en-e16E at 25^oC is increased from 28% to 44% if they are carrying mys⁸/+. Males expressing Fak56D^{Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^en-e16E at 25^oC and hemizygous for mys⁸ show 31% wing blistering (compared to 0% wing blistering in a mys⁺ background).

(Grabbe et al., 2004)

The addition of wb^4Y18/+ to mys⁸ hemizygotes enhances the wing blister phenotype, so that 28% of wings show a blistering phenotype. The addition of LanA^9-32 does not enhance this phenotype.

(Schock and Perrimon, 2003)

The addition of crol⁰⁴⁴¹⁸, crol⁰⁶⁴⁷⁰ does not enhance the wing blistering phenotype of mys⁸/Y flies. The addition of crol^k08217 does enhance the wing blistering phenotype of mys⁸/Y flies by 3 fold. The addition of crol^3ex5 enhances the wing blistering phenotype of mys⁸/Y flies by about 50%. This is significantly less than the enhancement seen by crol^k08217. The addition of EcR^C300Y or EcR^M554fs enhances the wing blistering phenotype of mys⁸/Y flies by about three or four-fold respectively. The amount of balloon wings is increased several fold in both cases.

(D'Avino and Thummel, 2000)

The frequency of wing blisters in mys⁸ hemizygotes is dominantly enhanced by bs^V100, cass^E48, puri^V4 or moa^V50. The frequency of wing blisters in mys⁸ hemizygotes is not dominantly enhanced by pyg^E16, pyg^E50, shot^V104, shot^V167, tak^V27, tak^V42, auk^V106, auk^V162, how^T1, how^T90, bs^V121, cass^E44, puri^V15, blo^V99, blo^V134 or moa^V88. The frequency of wing blisters seen in mys¹¹/mys⁸ flies is not dominantly enhanced by pot^P3.

(Walsh and Brown, 1998)

The frequency of wing blisters of mys⁸ hemizygotes is slightly increased (to approximately 20%) if the flies are also heterozygous for auk^unspecified, auk^2R-4, shot^unspecified, shot^65-2, blo^unspecified, blo^2R-17, kiwi^unspecified, kiwi^2R-3, mam^unspecified or 2R-L^84-2, moderately increased (to approximately 40%) if the flies are also heterozygous for bub^2L-2, and strongly increased if the flies are also heterozygous for bs^unspecified, rhea² or rhea¹. The frequency of the wings held-out phenotype is enhanced if the flies are also heterozygous for stck^unspecified, stck^3R-17, rhea¹, rhea², pio^2R-16 or pio^unspecified. Shows a weak genetic interaction with Dl^unspecified.

(Prout et al., 1997)

Individuals in combination with cno^mis1 exhibit incomplete lethality, few escapers have normal wings or an extra wing vein phenotype.

(Miyamoto et al., 1995)

Enhances the blister but not vein phenotype of bs mutations.

(Fristrom et al., 1994)

Double mutants with mys⁸ have wing blister phenotype.

(Brabant and Brower, 1993)

The held-out wing phenotype of mys⁸/mys^G flies is dominantly enhanced by bs^unspecified.

(Wessendorf et al., 1992)

Double hemizygous and homozygous mutants with if³ display an extreme wing phenotype, wings are folded and unexpanded, veins and aberrant crossveins can be seen.

(Wilcox et al., 1989)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

mys⁹⁶⁸

(Bateman et al., 2001)

mys^olfC-x17

(Ayyub et al., 2000)

mys^olfC-x10

(Ayyub et al., 2000)

mys^olfC-x5

(Ayyub et al., 2000)

mys^olfC-x3

(Ayyub et al., 2000)

Rescued by

mys⁸ is rescued by mys^hs.PB

(Longley and Ready, 1995)

mys⁸ is rescued by mys^rYYF

(Grinblat et al., 1994)

mys⁸ is rescued by mys^din

(Grinblat et al., 1994)

mys⁸ is rescued by mys^hs.PB

(Brabant and Brower, 1993)

Partially rescued by

mys⁸ is partially rescued by mys^tZa

(Grinblat et al., 1994)

mys⁸ is partially rescued by mys^rFFA

(Grinblat et al., 1994)

mys⁸ is partially rescued by mys^rDEA

(Grinblat et al., 1994)

mys⁸ is partially rescued by mys^4B

(Zusman et al., 1993)

mys⁸ is partially rescued by mys^tZa

(Zusman et al., 1993)

mys⁸ is partially rescued by mys^4A

(Zusman et al., 1993)

Not rescued by

mys⁸ is not rescued by mys^t2

(Grinblat et al., 1994)

mys⁸ is not rescued by mys^t1

(Grinblat et al., 1994)

Comments

mys⁸ fails to complement the isoamyl acetate olfactory response phenotype seen in mys^olfC-x3, mys^olfC-x5, mys^olfC-x10 and mys^olfC-x17.

(Ayyub et al., 2000)

The if³/mys⁸ wing blister phenotype can be rescued by a mys⁺ transgene.

(Brabant and Brower, 1993)

The held-out wing phenotype and blistered wing phenotype of males are rescued by mys^4A, mys^4B and mys^tZa.

(Zusman et al., 1993)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Altered PSβ protein.

(Wilcox et al., 1989)

Double heterozygotes of LanA^7-5/+ and mys⁸/+ indicated no interaction of the mutations.

(Henchcliffe et al., 1993)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

mys⁸

mys^NJ42

(Walsh and Brown, 1998)

mys^nj49

(Brown et al., 2002)

mys^nj

(Fristrom et al., 1994, Wessendorf et al., 1992)

nj42

nj⁴²

(de la Pompa et al., 1989, Digan et al., 1986)

Name Synonyms

Secondary FlyBase IDs

References (30)

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