Polytene chromosomes are normal (Foster, 1975).
H to Y substitution in the 29th EGF repeat of the N protein.
Mutation is within EGF element 29 of the N extracellular domain.
Amino acid replacement: H1167Y.
Amino acid replacement: H1167Y. Mutant partially sequenced; correlated with single amino acid replacements within six adjacent EGF-homologous elements of the N protein; has Gly at residue 2057 characteristic of Oregon R rather than Ser of Canton S (Kelley, Kidd, Deutsch and Young, 1987). Histidine replaced by tyrosine at residue 1167 in EGF-like repeat 29; CAT --> TAT (Hartley, Xu and Artavanis-Tsakonas, 1987; Kelley, Kidd, Deutsch and Young, 1987).
C3166127T
C?T
H1167Y | N-PA; H1167Y | N-PB
H1167Y
thorax & macrochaeta
Heterozygotes show a loss of wing veins.
NAx-E2 mutants exhibit a loss of the fourth and fifth wing veins in male flies caused by a gain of function of Notch.
Heterozygotes have a gap at the distal end of wing vein L5.
Mutants have shortened L4 and L5 wing veins.
Mutant flies have 90.88 +/- 1.94 thoracic microchaetae per heminotum (compared to the wild-type number of 130.35 +/- 1.54).
Heterozygotes show loss of wing vein, with a penetrance of 8.7%.
Shortened wing veins.
The number of missing bristles remains roughly equal with increasing temperature in homozygous flies, while the number and length of wing vein breaks increases with increasing temperature.
Some macrochaetae on the thorax are missing. Gaps in wing veins are present.
Length of microchaetae, macrochaetae and longitudinal wing veins is reduced with respect to that of wild type. Enhances the N haplo-insufficient phenotype of loss of the wing margin. Heteroallelic combinations with lethal Abruptex alleles show stronger phenotypes at 29oC than when heterozygous with a N null at 29oC.
Short wing veins IV and V.
Some hemizygous males occasionally lack the twin sensilla of the wing margin, the ventral sensillum of wing vein L3, and the anterior cross vein sensillum.
homozygous viable phenotype like NAx-1. Temperature sensitive for morphological phenotypes (Foster, 1975) but stable for viability (Portin and Siren, 1976). Viable in heterozygotes with N; Notch-wing phenotype is enhanced. At 18oC and 25oC, complementary in heterozygotes with recessive alleles at Notch; at 29oC, spl and Nnd-2 are weakly expressed (Portin, 1977a). Heterozygotes Nnd-3 spl NAx-E2/+++ are like spl/spl with suppression of wing-vein gaps; Nnd-3++/+ spl NAx-E2 and +spl NAx-E2/+++ show mild expression of spl (Foster, 1975). NAx-E2 is viable with alleles NAx-71d and NAx-16; lethal with lethal alleles NAx-59b and NAx-59d and with NAx-E1, NAx-9, and NAx-1 (Foster, 1975; Portin, 1975) and the lethality with NAx-1 is more pronounced at 29oC (Portin and Siren, 1976). In NAx-E2/NAx-1, the TSP for lethality is monophasic from the end of the third instar to early pupa (Portin and Siren, 1976). In NAx-E2/NAx-9, the focus of lethality is close to hypodermal sites of ventral thoracic structures, and in surviving gynandromorphs, the negative interaction between alleles is autonomous (Portin, 1977). NAx-E2 is placed on the genetic map of Notch close to and to the right of spl (Foster).
NAx-9/NAx-E2, dxENU/dx[+] has partially lethal - majority die phenotype, enhanceable by krzS095214
NAx-E2 has visible phenotype, enhanceable by Df(2L)Su(dx)-7
NAx-1/NAx-E2 has partially lethal phenotype, enhanceable by Dp(3;3)MKRS-D2
NAx-1/NAx-E2 has partially lethal phenotype, enhanceable by Dp(3;3)bxd110
NAx-9/NAx-E2 has partially lethal phenotype, enhanceable by Dp(3;3)MKRS-D2
NAx-9/NAx-E2 has partially lethal phenotype, enhanceable by Dp(3;3)bxd110
NAx-9/NAx-E2 has partially lethal - majority die phenotype, suppressible | partially by dxENU/dx[+]
NAx-9/NAx-E2 has lethal phenotype, suppressible by Df(3R)Delta-BX6
N[+]/NAx-E2 is a suppressor | partially of visible | heat sensitive phenotype of Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU
NAx-E2, Su(dx)sp has partially lethal - majority die phenotype
Dp(3;3)MKRS-D2, NAx-E2 has partially lethal phenotype
Dp(3;3)bxd110, NAx-E2 has partially lethal phenotype
NAx-E2 has wing vein phenotype, enhanceable by shamse01256/shamse01256
NAx-E2 has wing vein phenotype, enhanceable by Df(2L)Su(dx)-7
NAx-E2 has wing vein phenotype, enhanceable by E(spl)m8-HLH1
NAx-E2 has wing vein L4 phenotype, suppressible by Ero1L[+]/Ero1L23T
NAx-E2 has wing vein L5 phenotype, suppressible by Ero1L[+]/Ero1L23T
NAx-E2 has wing vein L5 | distal phenotype, suppressible by dx152/dx[+]
NAx-E2 has wing vein L4 phenotype, suppressible by Nipped-A[+]/Nipped-A323
NAx-E2 has wing vein L4 phenotype, suppressible by Nipped-A[+]/Nipped-ANC194
NAx-E2 has wing vein L4 phenotype, suppressible by domk08108/dom[+]
NAx-E2 has microchaeta phenotype, suppressible by dshhs.PA
NAx-E2 has phenotype, suppressible by Df(2R)Nipped-E43
NAx-E2 has wing vein phenotype, suppressible by Df(2R)Nipped-D341.1
NAx-E2 has wing vein phenotype, suppressible by Df(2R)Nipped-E338
NAx-E2 has wing vein phenotype, suppressible by Nipped-A222.3
NAx-E2 has wing vein phenotype, suppressible by RpL3845-72
NAx-E2 has macrochaeta phenotype, suppressible by E(spl)m8-HLH1
NAx-E2 has macrochaeta phenotype, suppressible by pydJ14
NAx-E2 has wing vein L4 phenotype, non-suppressible by Ada2b[+]/Ada2b2
NAx-E2 has wing vein L4 phenotype, non-suppressible by Nipped-A[+]/Nipped-ANC96
NAx-E2 has wing vein L4 phenotype, non-suppressible by Nipped-A[+]/Nipped-ANC105
NAx-E2 has wing vein L4 phenotype, non-suppressible by Nipped-A[+]/Nipped-ANC106
NAx-E2 has wing vein L4 phenotype, non-suppressible by Nipped-A[+]/Nipped-ANC186
NAx-E2 has wing vein L4 phenotype, non-suppressible by Ada2b[+]/Ada2bHD-5
NAx-E2 has wing vein L4 phenotype, non-suppressible by Ada2b[+]/Ada2b1
NAx-E2 has phenotype, non-suppressible by Nipped-B292.1
NAx-E2 has phenotype, non-suppressible by Nipped-B407
NAx-E2 is an enhancer of wing margin bristle phenotype of Cdc42N17.UAS, Scer\GAL4Bx-MS1096
NAx-E2 is an enhancer of wing margin phenotype of Cdc42N17.UAS, Scer\GAL4Bx-MS1096
N[+]/NAx-E2 is a suppressor | partially of wing blade posterior compartment | heat sensitive phenotype of Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU
NAx-E2 is a suppressor of phenotype of dxunspecified
The wing blistering phenotype seen in the posterior compartment of wings in flies expressing EogtGD5084 under the control of Scer\GAL4en.PU in the presence of Dcr-2Scer\UAS.cDa is dominantly partially suppressed if the flies are also heterozygous for NAx-E2.
Wing vein L4 is shortened in NAx-E2 mutants; heterozygosity for Nipped-A323, Nipped-ANC194 or domk08108 increases the length of L4.
The percentage of NAx-9/NAx-E2 flies surviving to adulthood is dramatically increased by dxENU/+. Survival of NAx-9/NAx-E2 dxENU/+ flies to adulthood is significantly decreased by krzS047819/+ or krzS095214/+.
Nipped-B407, l(2)41Ae7, Chie5.5 and vg1 have little or no effect on the NAx-E2 wing vein phenotype.
Homozygous viability is decreased if the flies also carry Dp(3;3)MKRS-D2 or Dp(3;3)bxd110.
Lethality of NAx-E2/NAx-9 heterozygotes can be rescued by Df(3R)Dl-BX6, Dl9P or mam10.
Foster.
Induced in: Oregon-R stock.
NAx-E2 shows negative complementation with NAx-M1.