FB2021_06, released December 14, 2021

Allele: Dmel\Nspl-1

Open Close
General Information
Symbol
Dmel\Nspl-1
Species
D. melanogaster
Name
FlyBase ID
FBal0012900
Feature type
allele
Associated gene
Dmel\N
Associated Insertion(s)
Carried in Construct
Also Known As
spl, Nspl, split, spl1, Nsp1
Key Links
Genomic Maps
Allele class

gain of function allele

antimorphic allele - genetic evidence

hypomorphic allele - genetic evidence

Mutagen
    Nature of the Allele
    Allele class

    antimorphic allele - genetic evidence

    (de Celis and Garcia-Bellido, 1994)

    gain of function allele

    (Hing et al., 1999)

    hypomorphic allele - genetic evidence

    (Brennan et al., 1997)
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    point_mutation
    X:3,164,361..3,164,361 [+]
    Nucleotide change:

    T3164361C

    Amino acid change:

    I578T | N-PA; I578T | N-PB

    Reported amino acid change:

    I578T

    Comment:

    Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

    (Lieber et al., 1992)
    Associated Sequence Data
    DDBJ / EMBL / GenBank
    DNA sequence
    Protein sequence
     
    UniProtKB/Swiss-Prot
      UniProtKB/TrEMBL
         
        Progenitor genotype
        Cytology

        Polytene chromosomes normal.

        (Lindsley and Zimm, 1992)
        Nature of the lesion
        Statement
        Reference

        Amino acid replacement: I578T.

        (Lieber et al., 1992)

        Mutation is within EGF-like repeat 14 of the N extracellular domain.

        (Lieber et al., 1992)

        Substitution of an isoleucine for a threonine in EGF-repeat number 14.

        (Baker et al., 1990)

        A missense mutation in the extracellular EGF-like domain.

        (Ramos et al., 1989)
        Expression Data
        Reporter Expression
        Additional Information
        Statement
        Reference
         
        Marker for
        Reflects expression of
        Reporter construct used in assay
        Human Disease Associations
        Disease Ontology (DO) Annotations
        Models Based on Experimental Evidence ( 0 )
        Disease
        Evidence
        References
        Modifiers Based on Experimental Evidence ( 0 )
        Disease
        Interaction
        References
        Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
         
        Disease-implicated variant(s)
         
        Phenotypic Data
        Phenotypic Class
        Phenotype Manifest In

        adult thoracic sensillum

        (Indrasamy et al., 2000, Brennan et al., 1997)

        chaeta

        (Xu et al., 1990)

        cone cell | third instar larval stage

        (Cordero and Cagan, 2010)

        dorsocentral bristle

        (Nagel and Preiss, 1999)

        eye

        (Cordero and Cagan, 2010, Kahali et al., 2009, Schaaf et al., 2009, Jones et al., 2006, Li et al., 2003, Indrasamy et al., 2000, Hing et al., 1999, Nagel and Preiss, 1999, Rollins et al., 1999, Lindsley and Zimm, 1992, Campos-Ortega and Knust, 1990, Rabinow and Birchler, 1990, Xu et al., 1990, Cagan and Ready, 1989, Shepard et al., 1989, Shellenbarger and Mohler, 1975, Cicak and Oster, 1957, Lewis, 1945)

        eye | heat sensitive

        (Foster, 1973)

        eye | male

        (Li and Baker, 2004)

        eye | somatic clone

        (Li et al., 2003)

        eye photoreceptor cell

        (Campos-Ortega and Knust, 1990)

        interommatidial bristle

        (Jones et al., 2006, Lai et al., 1998)

        interommatidial bristle | ectopic

        (Lai et al., 1998)

        macrochaeta

        (Hing et al., 1999, Rollins et al., 1999, de Celis and Garcia-Bellido, 1994, Lindsley and Zimm, 1992, Rabinow and Birchler, 1990, Shellenbarger and Mohler, 1975, Cicak and Oster, 1957, Meyer, 1952)

        macrochaeta | ectopic

        (Shellenbarger and Mohler, 1975)

        macrochaeta | heat sensitive

        (Foster, 1973)

        mesothoracic leg sensillum

        (Held, 1990)

        mesothoracic metatarsus

        (Held, 1990)

        mesothoracic tarsal bristle

        (Held, 1990)

        microchaeta

        (Ramain et al., 2001, Nagel and Preiss, 1999, de Celis and Garcia-Bellido, 1994)

        microchaeta | ectopic

        (Shellenbarger and Mohler, 1975)

        ocellar bristle

        (Shellenbarger and Mohler, 1975)

        ommatidial precursor cluster

        (Cagan and Ready, 1989)

        ommatidium

        (Jones et al., 2006, Nagel and Preiss, 1999, Campos-Ortega and Knust, 1990, Shellenbarger and Mohler, 1975)

        ommatidium & eye disc | male

        (Li and Baker, 2004)

        ommatidium | heat sensitive

        (Foster, 1973)

        ommatidium | somatic clone

        (Li et al., 2003)

        photoreceptor | third instar larval stage

        (Cordero and Cagan, 2010)

        photoreceptor cell & eye disc | male

        (Li and Baker, 2004)

        photoreceptor cell R7 | ectopic | somatic clone

        (Li et al., 2003)

        photoreceptor cell R8 & eye disc | male

        (Li and Baker, 2004)

        photoreceptor cell R8 | somatic clone

        (Li et al., 2003)

        presutural bristle

        (Shellenbarger and Mohler, 1975)

        scutellar bristle

        (Kahali et al., 2009, Schaaf et al., 2009, Indrasamy et al., 2000)

        scutellar bristle | ectopic

        (Kahali et al., 2009)

        sternopleural bristle

        (Indrasamy et al., 2000)

        supraalar bristle

        (Shellenbarger and Mohler, 1975)

        tarsal segment | heat sensitive

        (Foster, 1973)

        tormogen cell

        (Nagel and Preiss, 1999)

        vibrissae

        (Indrasamy et al., 2000)

        wing | heat sensitive

        (Foster, 1973)
        Detailed Description
        Statement
        Reference

        The retinas of Nspl-1 third instar larvae have a reduced number of photoreceptors and cone cells compared to wild type.

        The adult eyes of Nspl-1 mutants are reduced in size compared to controls.

        (Cordero and Cagan, 2010)

        Nspl-1/Y flies display a uniformly rough and reduced eye.

        85-90% of Nspl-1/Y flies exhibit split and missing scutellar macrochaetes, whereas they display ectopic scutellar macrochaetes with attenuated (~45%) severity.

        (Kahali et al., 2009)

        Mutants have scutellar bristle defects (missing or twinned bristles).

        (Schaaf et al., 2009)

        Mutants have a reduced, rough eye with missing or double bristles.

        (Jones et al., 2006)

        Hemizygous Nspl-1 males have small, rough eyes. Some R8 cells and ommatidia are missing, while other ommatidia have abnormal R8 cells and lack other photoreceptor cells in Nspl-1/Y eye imaginal discs.

        (Li and Baker, 2004)

        When Nspl-1 clones are made in the developing eye, animals have fewer ommatidia and ommatidia with less than the normal complement of differentiated cells. Cell death is increased in mutant eyes. Many R8 precursors are absent. In about 13% of ommatidia with Nspl-1 clones ectopic R7 photoreceptor cells are seen.

        (Li et al., 2003)

        Mutant flies have 120.22 +/- 1.34 thoracic microchaetae per heminotum (compared to the wild-type number of 130.35 +/- 1.54).

        (Ramain et al., 2001)

        Nspl-1 mutants exhibit rough and small eyes.

        Nspl-1 mutants exhibit elevated levels of asymmetry and reduced mean character size relative to Canton-S flies for thoracic bristles. These mutants also display significantly elevated asymmetry relative to Canton-S for scutellar, vibrissa and carnia as well as sternopleural bristles.

        (Indrasamy et al., 2000)

        Homozygotes show small rough eyes and split bristles.

        (Hing et al., 1999)

        Dorsocentral bristles appear split due to a duplication of the bristle shaft at the expense of the socket cell. Conversely, at the back of the head, double sockets appear instead of microchaetae. Homozygous females have smaller eyes of rough appearance due to irregular spacing of ommatidia.

        (Nagel and Preiss, 1999)

        Small rough eyes, twinned bristles and missing bristles.

        (Rollins et al., 1999)

        Heterozygous females have largely wild-type eyes, although missing or double interommatidial bristles are sometimes seen.

        (Lai et al., 1998)

        Heterozygotes have a neurogenic phenotype and have an increase in the number of thoracic bristles compared to wild-type. The number of bristles is restored almost to wild-type in Nspl-1; Dp(1;2)51b double heterozygotes.

        (Brennan et al., 1997)

        Eye size of Nspl-1 is increased in males that are homozygous for mle4.

        (Hiebert and Birchler, 1994)

        Macrochaetae and microchaetae lost or affected by abnormal differentiation are those that are most sensitive to Abruptex mutants. In combination with a N null allele, Nspl-1 causes extra macrochaetae and microchaetae. In presence of a N duplication, the mutant phenotype is reduced but not suppressed.

        (de Celis and Garcia-Bellido, 1994)

        Clones mutant for spl on the thorax display a loss of function phenotype: more bristles are formed.

        (Heitzler and Simpson, 1993)

        Nspl-1 heterozygotes have essentially wild-type eyes. Nspl-1 heterozygotes also carrying EgfrE3 or EgfrE1 have rougher eyes. Hemizygous Nspl-1 animals homozygous for EgfrE3 or EgfrE1 have almost no facets, the eyes being composed almost entirely of pigment cells and bristles.

        (Baker and Rubin, 1992)

        Individuals homozygous for Bpt1 and Nspl-1 exhibit no Bpt1 phenotype and an enhanced spl phenotype.

        (Duus et al., 1992)

        Small rough eyes and missing or doubled bristles.

        (Gorman and Girton, 1992)

        Eyes rough and small. Many bristles doubled, sometimes missing. Bristle effect caused by an extra division of initial bristle-forming cell (FBrf0005804). Few bristles (but not their sockets) regularly removed from posterior border of tergites in Nspl-1/+ heterozygotes (Welshons). N64d6 Nspl-1 flies cannot be distinguished from N64d6/+ flies. When Nspl-1 is coupled to N64d6, Nspl-1 is not enhanced by E(spl)1. A temperature-sensitive effect is shown by N264-103/Nspl-1 flies, which have abnormal eye facets at 28-29oC but are almost wild type at 20-22oC. The spl phenotype can be enhanced by E(spl)1/+ or E(spl)1/E(spl)1. Nspl-1/+;E(spl)1/+ flies resemble Nspl-1/Nspl-1 flies; Nspl-1/Nspl-1; E(spl)1/+ and Nspl-1/Y;E(spl)1/+ flies show a very extreme mutant phenotype. Nspl-1/Y; groE73/+ males and Nspl-1/+; groE73/+ females show spl and Ax-like phenotypes (Xu et al.). The spl phenotype is reduced in mam heterozygotes. When, however, Nspl-1 is coupled to a N point mutant, as in N64d6 Nspl-1/++; E(spl)1/+, the phenotype is not spl. The Nspl-1 phenotype becomes dominant if Nspl-1 is coupled, in cis, to lethal Ax alleles. RK1.

        (Lindsley and Zimm, 1992)

        Phenotypes have topographic pattern specificities. Homozygotes show duplications and absence of trichogens preferentially in posterior and medial regions of the tergites.

        (Mari-Beffa et al., 1991)

        Increase in microchaetae density in the mesonotum when in combination with N amorph alleles and absence of macrochaetae when homozygote.

        (de Celis et al., 1991)

        Eye discs contain well-spaced clusters of cells with apically disposed nuclei, the number and arrangement of the cells is grossly abnormal. More than one boss-expressing cell was observed within many clusters. Prior to row 6 many cells per cluster contain boss-immunoreactive multivesicular bodies, boss is internalized as soon as boss expression is present on an adjacent cell surface. This correlates with a decrease in the number of R1-R6 neurons and an increase in the number of R7 neurons.

        (van Vactor et al., 1991)

        Homozygotes and hemizygotes have rough eyes which are slightly reduced in size compared to wild-type. Individual ommatidia are missing and ommatidia often contain fewer photoreceptor cells than normal and have defects in other ommatidial cell types. Photoreceptor cell axons appear normal.

        (Campos-Ortega and Knust, 1990)

        The total number of bristles on the basitarsi of the second legs is increased and the number of sensilla is decreased. A number of shaftless, misshapen sockets are seen.

        (Held, 1990)

        Homozygotes have small rough eyes due to reduced number of facets and twinned or missing bristles (FBrf0049795). Eye phenotype can be suppressed by sca and enhanced by extra copies of sca+ and vg+, the bristle phenotype is unaffected.

        (Rabinow and Birchler, 1990)

        Pronounced eye phenotype. Does not interact with dxENU.

        (Xu and Artavanis-Tsakonas, 1990)

        Small rough eyes and duplicated bristles.

        (Xu et al., 1990)

        Haploabnormal wing phenotype.

        (Alton et al., 1989)

        Early ommatidia in homozygous third instar discs are often poorly arranged and contain an abnormal number of cells. Rarely a single photoreceptor cell is found, and mitotic figures occasionally occur abnormally close to the furrow. By midpupation ommatidia often contain abnormal numbers of photoreceptors, cone cells and secondary pigment cells. Bristles are scattered and photoreceptors can be found beneath the retina. The eye is smaller than normal. Temperature shifts of third-instar Nl1N-ts1 larvae phenocopy the external defects of Nspl-1.

        (Cagan and Ready, 1989)

        Adult eyes rough, slightly reduced, with irregular facets and multiple setae. Irregular size, shape and composition of ommatidia: fewer than normal rhabdomeres per ommatidium. Eye disc pattern, shape and size irregular.

        (Renfranz and Benzer, 1989)

        Heterozygoutes have rough eyes. Hemizygotes show a more extreme reduction in eye size than homozygotes as well as an increase in facet and bristle abnormalities. Nspl-1/Y; E(spl)1/+ flies show a very extreme mutant phenotype.

        (Shepard et al., 1989)

        Dp(2;Y)G44 males crossed to a stock carrying Nspl-1 produce sons that have straight wings or curly wings. Curly wings result from trisomy for the 44C--50B region: enhance the mutant effect of Nspl-1.

        (Birchler, 1988)

        The split phenotype becomes dominant if Nspl-1 is coupled, in cis, to lethal Ax alleles.

        (Kelley et al., 1987)

        Homozygotes and hemizygotes develop small and rough compound eyes. Some macrochaetae are split or completely missing. The presence of one copy of E(spl)1 causes Nspl-1 to acquire a dominant phenotypic expression in all its traits. Dp(3;3)Su8 exerts a slight enhancing effect of the Nspl-1 phenotype.

        (Knust et al., 1987)

        Homozygous have small eyes with a rough, pebbly surface. Some bristles are doubled, while others, such as the ocellar, supraalar and presutural bristles are missing, and some extra hairs are present. These phenotypes are the same at 18, 25 and 29oC.

        (Shellenbarger and Mohler, 1975)

        Hemizygotes and homozygotes have reduced eyes with an irregular facet arrangement, and missing or doubled bristles are frequently seen. N264-103/Nspl-1 flies show wing nicking and tarsal segment fusion phenotypes and also show the Nspl-1 eye phenotype. The mutant eye and wing phenotypes are more strongly expressed at high temperatures than low temperatures, and tarsal fusion only occurs at high temperatures. At 20-22oC, the eyes approach wild type but usually contain small, irregularly positioned areas of facet disarray, while at 28-29oC the facet disarray extends over the whole surface of the eye. The temperature sensitive periods of these phenotypes occur during the third larval instar stage.

        (Foster, 1973)

        The split phenotype becomes dominant if Nspl-1 is coupled, in cis, to lethal Ax alleles. When Nspl-1 is coupled to a N point mutant, as in N64d6,Nspl-1/++; E(spl)1/+, the phenotype is not split.

        (Welshons, 1971)

        N68j/Nspl-1 flies have a Nspl-1 phenotype.

        (Hayman and Maddern, 1969)

        NM/Nspl-1 flies have roughened eyes which are smaller than normal and split bristles.

        (Cicak and Oster, 1957)

        N47i/Nspl-1 flies have a bristle phenotype but not a roughened eye phenotype. No phenotype is seen in N51d/Nspl-1 flies.

        (Meyer, 1952)

        Homozygotes have rough eyes.

        (Lewis, 1945)
        External Data
        Interactions
        Show genetic interaction network for Enhancers & Suppressors
        Phenotypic Class
        Enhanced by
        Statement
        Reference

        Nspl-1 has visible phenotype, enhanceable by vtd80Fh-36/vtd[+]

        (Schaaf et al., 2009)

        Nspl-1 has visible phenotype, enhanceable by vtdγ26-6/vtd[+]

        (Schaaf et al., 2009)

        Nspl-1 has visible | dominant phenotype, enhanceable by E(spl)m8-HLH1

        (Nagel and Preiss, 1999)

        E(spl)m8-HLH1, Nspl-1 has visible | dominant phenotype, enhanceable by ato5

        (Nagel and Preiss, 1999)

        E(spl)m8-HLH1, Nspl-1 has visible | dominant phenotype, enhanceable by ato3

        (Nagel and Preiss, 1999)

        E(spl)m8-HLH1, Nspl-1 has visible | dominant phenotype, enhanceable by da5

        (Nagel and Preiss, 1999)

        E(spl)m8-HLH1, Nspl-1 has visible | suppressible phenotype, enhanceable by scaunspecified

        (Nagel and Preiss, 1999)

        Nspl-1 has visible | dominant phenotype, enhanceable by E(spl)m8-HLHK:CAACdel

        (Lai et al., 1998)

        Nspl-1 has visible | dominant phenotype, enhanceable by E(spl)m8-HLHK1K2mut

        (Lai et al., 1998)

        Nspl-1 has visible | dominant phenotype, enhanceable by Df(3R)Dl-FX3

        (Brennan et al., 1997)

        Nspl-1 has visible phenotype, enhanceable by E(spl)m8-HLH1

        (Campos-Ortega and Knust, 1990)

        Nspl-1 has visible phenotype, enhanceable by E(spl)[+]/E(spl)m8-HLH1

        (Shepard et al., 1989)
        NOT Enhanced by
        Statement
        Reference

        Nspl-1 has visible | dominant phenotype, non-enhanceable by E(spl)m8-HLHtLa

        (Lai et al., 1998)
        Suppressed by
        Statement
        Reference

        Nspl-1 has abnormal size | adult stage phenotype, suppressible | partially by wg[+]/wgl-17

        (Cordero and Cagan, 2010)

        Nspl-1 has decreased cell number | third instar larval stage phenotype, suppressible | partially by wg[+]/wgl-17

        (Cordero and Cagan, 2010)

        Nspl-1 has visible phenotype, suppressible by snamaPX1/mnm[+]

        (Jones et al., 2006)

        Nspl-1 has visible phenotype, suppressible by l(2)rQ313[+]/snamarQ313

        (Jones et al., 2006)

        Nspl-1 has visible | recessive phenotype, suppressible by Gp150[+]/Gp150k11120b

        (Li et al., 2003)

        Nspl-1 has visible phenotype, suppressible by dshhs.PA

        (Ramain et al., 2001)

        Nspl-1 has visible | dominant phenotype, suppressible by wgS107

        (Brennan et al., 1997)

        E(spl)[+]/E(spl)m8-HLH1, Nspl-1 has visible phenotype, suppressible by DlBE21/Dl[+]

        (Shepard et al., 1989)

        E(spl)[+]/E(spl)m8-HLH1, Nspl-1 has visible phenotype, suppressible by DlBE35/Dl[+]

        (Shepard et al., 1989)

        E(spl)[+]/E(spl)m8-HLH1, Nspl-1 has visible phenotype, suppressible by DlBX40/Dl[+]

        (Shepard et al., 1989)

        E(spl)[+]/E(spl)m8-HLH1, Nspl-1 has visible phenotype, suppressible by Dl[+]/DlBX41

        (Shepard et al., 1989)

        E(spl)[+]/E(spl)m8-HLH1, Nspl-1 has visible phenotype, suppressible by Dl[+]/DlBX43

        (Shepard et al., 1989)

        E(spl)[+]/E(spl)m8-HLH1, Nspl-1 has visible phenotype, suppressible by DlBX45/Dl[+]

        (Shepard et al., 1989)

        E(spl)[+]/E(spl)m8-HLH1, Nspl-1 has visible phenotype, suppressible by DlBX46/Dl[+]

        (Shepard et al., 1989)

        E(spl)[+]/E(spl)m8-HLH1, Nspl-1 has visible phenotype, suppressible by Dl[+]/DlCE21

        (Shepard et al., 1989)

        E(spl)[+]/E(spl)m8-HLH1, Nspl-1 has visible phenotype, suppressible by DlCE23/Dl[+]

        (Shepard et al., 1989)

        E(spl)[+]/E(spl)m8-HLH1, Nspl-1 has visible phenotype, suppressible by DlCE33/Dl[+]

        (Shepard et al., 1989)

        E(spl)[+]/E(spl)m8-HLH1, Nspl-1 has visible phenotype, suppressible by DlCE34/Dl[+]

        (Shepard et al., 1989)

        E(spl)[+]/E(spl)m8-HLH1, Nspl-1 has visible phenotype, suppressible by Dl[+]/DlBE23

        (Shepard et al., 1989)

        E(spl)[+]/E(spl)m8-HLH1, Nspl-1 has visible phenotype, suppressible by DlCE37/Dl[+]

        (Shepard et al., 1989)

        E(spl)[+]/E(spl)m8-HLH1, Nspl-1 has visible phenotype, suppressible by Dl[+]/DlCE43

        (Shepard et al., 1989)

        E(spl)[+]/E(spl)m8-HLH1, Nspl-1 has visible phenotype, suppressible by DlBE24/Dl[+]

        (Shepard et al., 1989)

        E(spl)[+]/E(spl)m8-HLH1, Nspl-1 has visible phenotype, suppressible by DlBE26/Dl[+]

        (Shepard et al., 1989)

        E(spl)[+]/E(spl)m8-HLH1, Nspl-1 has visible phenotype, suppressible by DlBE32/Dl[+]

        (Shepard et al., 1989)
        NOT suppressed by
        Statement
        Reference

        E(spl)[+]/E(spl)m8-HLH1, Nspl-1 has visible phenotype, non-suppressible by DlHD82/Dl[+]

        (Shepard et al., 1989)
        Suppressor of
        Statement
        Reference

        N+/Nspl-1 is a suppressor | partially of visible | heat sensitive phenotype of Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU

        (Müller et al., 2013)
        Other
        Statement
        Reference

        Nspl-1, sno1 has lethal phenotype

        (Lefevre and Peterson, 1972)
        Phenotype Manifest In
        Enhanced by
        Statement
        Reference

        Nspl-1 has eye phenotype, enhanceable by E(spl)m8-HLH1.UAS/Scer\GAL4h-H10

        (Kahali et al., 2009)

        Nspl-1 has eye phenotype, enhanceable by vtd80Fh-36/vtd[+]

        (Schaaf et al., 2009)

        Nspl-1 has scutellar bristle phenotype, enhanceable by vtd80Fh-36/vtd[+]

        (Schaaf et al., 2009)

        Nspl-1 has eye phenotype, enhanceable by vtdγ26-6/vtd[+]

        (Schaaf et al., 2009)

        Nspl-1 has scutellar bristle phenotype, enhanceable by vtdγ26-6/vtd[+]

        (Schaaf et al., 2009)

        Nspl-1 has photoreceptor cell R8 phenotype, enhanceable by Scer\GAL4GMR.PF/DlUAS.cLa/Scer\GAL4GMR.PF

        (Li et al., 2003)

        Nspl-1 has eye phenotype, enhanceable by E(spl)m8-HLH1

        (Nagel and Preiss, 1999, Campos-Ortega and Knust, 1990)

        Nspl-1 has ommatidium phenotype, enhanceable by E(spl)m8-HLH1

        (Nagel and Preiss, 1999, Campos-Ortega and Knust, 1990)

        Nspl-1 has eye phenotype, enhanceable by E(spl)m8-HLH1.UAS/Scer\GAL4h-1J3

        (Nagel et al., 1999)

        Nspl-1 has eye phenotype, enhanceable by E(spl)m8-HLH::E(spl)mδ-HLHcNa

        (Nagel et al., 1999)

        Nspl-1 has eye phenotype, enhanceable by E(spl)m8-HLH::E(spl)m5-HLHE(spl).PN

        (Nagel et al., 1999)

        Nspl-1 has eye phenotype, enhanceable by E(spl)m8-HLH+t2.8

        (Nagel et al., 1999)

        Nspl-1 has eye phenotype, enhanceable by E(spl)m8-HLH1.tNa

        (Nagel et al., 1999)

        Nspl-1 has eye phenotype, enhanceable by E(spl)m7-HLH+t5.6

        (Nagel et al., 1999)

        Nspl-1 has phenotype, enhanceable by Df(2R)Nipped-D263.3

        (Rollins et al., 1999)

        Nspl-1 has phenotype, enhanceable by Df(2R)Nipped-E43

        (Rollins et al., 1999)

        Nspl-1 has macrochaeta phenotype, enhanceable by Df(2R)Nipped-D341.1

        (Rollins et al., 1999)

        Nspl-1 has macrochaeta phenotype, enhanceable by Df(2R)Nipped-E338

        (Rollins et al., 1999)

        Nspl-1 has interommatidial bristle | ectopic phenotype, enhanceable by E(spl)m8-HLHK:CAACdel

        (Lai et al., 1998)

        Nspl-1 has ommatidium phenotype, enhanceable by E(spl)m8-HLHK:CAACdel

        (Lai et al., 1998)

        Nspl-1 has interommatidial bristle | ectopic phenotype, enhanceable by E(spl)m8-HLHK1K2mut

        (Lai et al., 1998)

        Nspl-1 has ommatidium phenotype, enhanceable by E(spl)m8-HLHK1K2mut

        (Lai et al., 1998)

        Nspl-1 has adult thoracic sensillum phenotype, enhanceable by Df(3R)Dl-FX3

        (Brennan et al., 1997)

        Nspl-1 has phenotype, enhanceable by E(spl)m8-HLH1.tKa

        (Tietze et al., 1993)

        Nspl-1 has phenotype, enhanceable by rn[+]/rnroe-KE17

        (Brand and Campos-Ortega, 1990)

        Nspl-1 has phenotype, enhanceable by rn[+]/rnroe-KE18

        (Brand and Campos-Ortega, 1990)

        Nspl-1 has phenotype, enhanceable by rnroe-KE27/rn[+]

        (Brand and Campos-Ortega, 1990)

        Nspl-1 has phenotype, enhanceable by rnroe-KE55/rn[+]

        (Brand and Campos-Ortega, 1990)

        Nspl-1 has phenotype, enhanceable by rn[+]/rnroe-KE100

        (Brand and Campos-Ortega, 1990)

        Nspl-1 has phenotype, enhanceable by rnroe-KE345/rn[+]

        (Brand and Campos-Ortega, 1990)

        Nspl-1 has phenotype, enhanceable by rnroe-KE405/rn[+]

        (Brand and Campos-Ortega, 1990)

        Nspl-1 has phenotype, enhanceable by rnroe-KEK2/rn[+]

        (Brand and Campos-Ortega, 1990)

        Nspl-1 has phenotype, enhanceable by rnroe-KX314/rn[+]

        (Brand and Campos-Ortega, 1990)

        Nspl-1 has phenotype, enhanceable by rnroe-KE89/rn[+]

        (Brand and Campos-Ortega, 1990)

        Nspl-1 has phenotype, enhanceable by rn[+]/rnroe-KE113

        (Brand and Campos-Ortega, 1990)

        Nspl-1 has phenotype, enhanceable by rn[+]/rnroe-KE380

        (Brand and Campos-Ortega, 1990)

        Nspl-1 has phenotype, enhanceable by rnroe-KE399/rn[+]

        (Brand and Campos-Ortega, 1990)

        Nspl-1 has phenotype, enhanceable by rnroe-KE80/rn[+]

        (Brand and Campos-Ortega, 1990)

        Nspl-1 has phenotype, enhanceable by rn[+]/rnroe-KE350

        (Brand and Campos-Ortega, 1990)

        Nspl-1 has phenotype, enhanceable by rnroe-KE409/rn[+]

        (Brand and Campos-Ortega, 1990)

        Nspl-1 has phenotype, enhanceable by rn[+]/rnroe-KX316

        (Brand and Campos-Ortega, 1990)

        Nspl-1 has eye phenotype, enhanceable by E(spl)[+]/E(spl)m8-HLH1

        (Shepard et al., 1989)

        Nspl-1 has phenotype, enhanceable by E(spl)m8-HLHrv29

        (Ziemer et al., 1988)

        Nspl-1 has phenotype, enhanceable by E(spl)m8-HLHrv30

        (Ziemer et al., 1988)

        Nspl-1 has phenotype, enhanceable by E(spl)m8-HLHrv31

        (Ziemer et al., 1988)
        NOT Enhanced by
        Statement
        Reference

        Nspl-1 has scutellar bristle | ectopic phenotype, non-enhanceable by E(spl)m8-HLH1.UAS/Scer\GAL4h-H10

        (Kahali et al., 2009)

        Nspl-1 has scutellar bristle phenotype, non-enhanceable by E(spl)m8-HLH1.UAS/Scer\GAL4h-H10

        (Kahali et al., 2009)

        Nspl-1 has eye phenotype, non-enhanceable by E(spl)m8-HLHΔgro.UAS/Scer\GAL4h-H10

        (Kahali et al., 2009)

        Nspl-1 has eye phenotype, non-enhanceable by E(spl)m8-HLHSA.Δgro.UAS/Scer\GAL4h-H10

        (Kahali et al., 2009)

        Nspl-1 has eye phenotype, non-enhanceable by E(spl)m8-HLHSD.Δgro.UAS/Scer\GAL4GMR.PU

        (Kahali et al., 2009)

        Nspl-1 has eye phenotype, non-enhanceable by E(spl)m8-HLHΔgro.UAS/Scer\GAL4sca-109-68

        (Kahali et al., 2009)

        Nspl-1 has eye phenotype, non-enhanceable by E(spl)m8-HLHSA.Δgro.UAS/Scer\GAL4sca-109-68

        (Kahali et al., 2009)

        Nspl-1 has scutellar bristle | ectopic phenotype, non-enhanceable by Df(3R)Espl22/+

        (Kahali et al., 2009)

        Nspl-1 has scutellar bristle | ectopic phenotype, non-enhanceable by Scer\GAL4h-H10/CkIIαdsRNA.UAS

        (Kahali et al., 2009)

        Nspl-1 has eye phenotype, non-enhanceable by E(spl)m8-HLH1.UAS/Scer\GAL4sca-109-68

        (Kahali et al., 2009)

        Nspl-1 has eye phenotype, non-enhanceable by E(spl)m8-HLH1.UAS/Scer\GAL4h-H10

        (Bose et al., 2006)

        Nspl-1 has eye phenotype, non-enhanceable by Egfrf3

        (Nagel and Preiss, 1999)

        Nspl-1 has eye phenotype, non-enhanceable by S54

        (Nagel and Preiss, 1999)

        Nspl-1 has eye phenotype, non-enhanceable by SIIN

        (Nagel and Preiss, 1999)

        Nspl-1 has eye phenotype, non-enhanceable by hh18

        (Nagel and Preiss, 1999)

        Nspl-1 has eye phenotype, non-enhanceable by hh21

        (Nagel and Preiss, 1999)

        Nspl-1 has eye phenotype, non-enhanceable by hh3

        (Nagel and Preiss, 1999)

        Nspl-1 has eye phenotype, non-enhanceable by wgl-12

        (Nagel and Preiss, 1999)

        Nspl-1 has eye phenotype, non-enhanceable by wgl-9

        (Nagel and Preiss, 1999)

        Nspl-1 has eye phenotype, non-enhanceable by E(spl)m8-HLHUAS.cGa/Scer\GAL4h-1J3

        (Nagel et al., 1999)

        Nspl-1 has eye phenotype, non-enhanceable by E(spl)m8-HLH::E(spl)m5-HLHcNa

        (Nagel et al., 1999)

        Nspl-1 has phenotype, non-enhanceable by Chie5.5

        (Rollins et al., 1999)

        Nspl-1 has phenotype, non-enhanceable by Nipped-A222.3

        (Rollins et al., 1999)

        Nspl-1 has phenotype, non-enhanceable by Su(H)8

        (Rollins et al., 1999)

        Nspl-1 has phenotype, non-enhanceable by vg1

        (Rollins et al., 1999)

        Nspl-1 has macrochaeta phenotype, non-enhanceable by Nipped-B292.1

        (Rollins et al., 1999)

        Nspl-1 has macrochaeta phenotype, non-enhanceable by Su(H)16

        (Rollins et al., 1999)

        Nspl-1 has interommatidial bristle phenotype, non-enhanceable by E(spl)m8-HLHtLa

        (Lai et al., 1998)

        Nspl-1 has ommatidium phenotype, non-enhanceable by E(spl)m8-HLHtLa

        (Lai et al., 1998)

        Nspl-1 has phenotype, non-enhanceable by E(S)1

        (Lewis, 1945)
        Suppressed by
        Statement
        Reference

        Nspl-1 has eye phenotype, suppressible | partially by wg[+]/wgl-17

        (Cordero and Cagan, 2010)

        Nspl-1 has cone cell phenotype, suppressible | partially by wg[+]/wgl-17

        (Cordero and Cagan, 2010)

        Nspl-1 has photoreceptor phenotype, suppressible | partially by wg[+]/wgl-17

        (Cordero and Cagan, 2010)

        Nspl-1 has eye phenotype, suppressible by Scer\GAL4sca-109-68/E(spl)m8-HLHSD.Δgro.UAS

        (Kahali et al., 2009)

        Nspl-1 has eye phenotype, suppressible by Df(3R)Espl22/+

        (Kahali et al., 2009)

        Nspl-1 has scutellar bristle phenotype, suppressible by Df(3R)Espl22/+

        (Kahali et al., 2009)

        Nspl-1 has eye phenotype, suppressible by Scer\GAL4h-H10/CkIIαdsRNA.UAS

        (Kahali et al., 2009)

        Nspl-1 has scutellar bristle phenotype, suppressible by Scer\GAL4h-H10/CkIIαdsRNA.UAS

        (Kahali et al., 2009)

        Nspl-1 has eye phenotype, suppressible by CkIIαUAS.Tik/Scer\GAL4h-H10

        (Kahali et al., 2009)

        Nspl-1 has eye phenotype, suppressible by E(spl)m8-HLHSD.Δgro.UAS/Scer\GAL4h-H10

        (Kahali et al., 2009)

        Nspl-1 has scutellar bristle | ectopic phenotype, suppressible by E(spl)m8-HLHSD.Δgro.UAS/Scer\GAL4h-H10

        (Kahali et al., 2009)

        Nspl-1 has scutellar bristle phenotype, suppressible by E(spl)m8-HLHSD.Δgro.UAS/Scer\GAL4h-H10

        (Kahali et al., 2009)

        Nspl-1 has eye phenotype, suppressible by Nipped-B[+]/Nipped-B407

        (Schaaf et al., 2009)

        Nspl-1 has eye phenotype, suppressible by snamaPX1/mnm[+]

        (Jones et al., 2006)

        Nspl-1 has ommatidium phenotype, suppressible by snamaPX1/mnm[+]

        (Jones et al., 2006)

        Nspl-1 has eye phenotype, suppressible by l(2)rQ313[+]/snamarQ313

        (Jones et al., 2006)

        Nspl-1 has ommatidium phenotype, suppressible by l(2)rQ313[+]/snamarQ313

        (Jones et al., 2006)

        Nspl-1 has eye phenotype, suppressible by Gp150[+]/Gp150k11120b

        (Li et al., 2003)

        Nspl-1 has microchaeta phenotype, suppressible by dshhs.PA

        (Ramain et al., 2001)

        Nspl-1 has eye phenotype, suppressible by Hsc70-4195

        (Hing et al., 1999)

        Nspl-1 has macrochaeta phenotype, suppressible by Hsc70-4195

        (Hing et al., 1999)

        Nspl-1 has phenotype, suppressible by Df(2R)Nipped-D263.3

        (Rollins et al., 1999)

        Nspl-1 has eye phenotype, suppressible by Df(2R)Nipped-D341.1

        (Rollins et al., 1999)

        Nspl-1 has eye phenotype, suppressible by Df(2R)Nipped-E338

        (Rollins et al., 1999)

        Nspl-1 has eye phenotype, suppressible by Nipped-B292.1

        (Rollins et al., 1999)

        Nspl-1 has eye phenotype, suppressible by Nipped-B407

        (Rollins et al., 1999)

        Nspl-1 has eye phenotype, suppressible by Nipped-A323

        (Rollins et al., 1999)

        Nspl-1 has eye phenotype, suppressible by RpL3845-72

        (Rollins et al., 1999)

        Nspl-1 has eye phenotype, suppressible by Su(H)16

        (Rollins et al., 1999)

        Nspl-1 has adult thoracic sensillum phenotype, suppressible by wgS107

        (Brennan et al., 1997)

        Nspl-1 has phenotype, suppressible by E(nd)195ry+3550

        (Hing and Artavanis-Tsakonas, 1995)

        Nspl-1 has phenotype, suppressible by Su(spl)KE7

        (Hu et al., 1995, Mlodzik et al., 1990)

        Nspl-1 has phenotype, suppressible by Dlsup4

        (Lieber et al., 1992)

        Nspl-1 has phenotype, suppressible by Dlsup5

        (Lieber et al., 1992)

        Nspl-1 has phenotype, suppressible by scaKE15

        (Mlodzik et al., 1990)

        Nspl-1 has phenotype, suppressible by scaKE17

        (Mlodzik et al., 1990)

        Nspl-1 has phenotype, suppressible by scaKE305

        (Mlodzik et al., 1990)

        Nspl-1 has phenotype, suppressible by scaKE3

        (Mlodzik et al., 1990)

        Nspl-1 has phenotype, suppressible by scaKX2

        (Mlodzik et al., 1990)

        Nspl-1 has eye phenotype, suppressible by mam10/mam[+]

        (Xu et al., 1990)

        Nspl-1 has chaeta phenotype, suppressible by mam10/mam[+]

        (Xu et al., 1990)

        Nspl-1 has eye phenotype, suppressible by mam88-4/mam[+]

        (Xu et al., 1990)

        Nspl-1 has chaeta phenotype, suppressible by mam88-4/mam[+]

        (Xu et al., 1990)

        Nspl-1 has eye phenotype, suppressible | partially by mam88-10/mam[+]

        (Xu et al., 1990)

        Nspl-1 has chaeta phenotype, suppressible | partially by mam88-10/mam[+]

        (Xu et al., 1990)

        E(spl)[+]/E(spl)m8-HLH1, Nspl-1 has eye phenotype, suppressible by DlBE32/Dl[+]

        (Shepard et al., 1989)

        E(spl)[+]/E(spl)m8-HLH1, Nspl-1 has eye phenotype, suppressible by DlBE35/Dl[+]

        (Shepard et al., 1989)

        E(spl)[+]/E(spl)m8-HLH1, Nspl-1 has eye phenotype, suppressible by DlBX40/Dl[+]

        (Shepard et al., 1989)

        E(spl)[+]/E(spl)m8-HLH1, Nspl-1 has eye phenotype, suppressible by Dl[+]/DlBX41

        (Shepard et al., 1989)

        E(spl)[+]/E(spl)m8-HLH1, Nspl-1 has eye phenotype, suppressible by Dl[+]/DlBX43

        (Shepard et al., 1989)

        E(spl)[+]/E(spl)m8-HLH1, Nspl-1 has eye phenotype, suppressible by DlBE21/Dl[+]

        (Shepard et al., 1989)

        E(spl)[+]/E(spl)m8-HLH1, Nspl-1 has eye phenotype, suppressible by DlBX45/Dl[+]

        (Shepard et al., 1989)

        E(spl)[+]/E(spl)m8-HLH1, Nspl-1 has eye phenotype, suppressible by DlBX46/Dl[+]

        (Shepard et al., 1989)

        E(spl)[+]/E(spl)m8-HLH1, Nspl-1 has eye phenotype, suppressible by Dl[+]/DlCE21

        (Shepard et al., 1989)

        E(spl)[+]/E(spl)m8-HLH1, Nspl-1 has eye phenotype, suppressible by DlCE23/Dl[+]

        (Shepard et al., 1989)

        E(spl)[+]/E(spl)m8-HLH1, Nspl-1 has eye phenotype, suppressible by DlCE33/Dl[+]

        (Shepard et al., 1989)

        E(spl)[+]/E(spl)m8-HLH1, Nspl-1 has eye phenotype, suppressible by DlCE34/Dl[+]

        (Shepard et al., 1989)

        E(spl)[+]/E(spl)m8-HLH1, Nspl-1 has eye phenotype, suppressible by DlCE37/Dl[+]

        (Shepard et al., 1989)

        E(spl)[+]/E(spl)m8-HLH1, Nspl-1 has eye phenotype, suppressible by Dl[+]/DlCE43

        (Shepard et al., 1989)

        E(spl)[+]/E(spl)m8-HLH1, Nspl-1 has eye phenotype, suppressible by Dl[+]/DlBE23

        (Shepard et al., 1989)

        E(spl)[+]/E(spl)m8-HLH1, Nspl-1 has eye phenotype, suppressible by DlBE24/Dl[+]

        (Shepard et al., 1989)

        E(spl)[+]/E(spl)m8-HLH1, Nspl-1 has eye phenotype, suppressible by DlBE26/Dl[+]

        (Shepard et al., 1989)
        NOT suppressed by
        Statement
        Reference

        Nspl-1 has eye phenotype, non-suppressible by E(spl)m8-HLHSA.Δgro.UAS/Scer\GAL4h-H10

        (Kahali et al., 2009)

        Nspl-1 has scutellar bristle | ectopic phenotype, non-suppressible by E(spl)m8-HLHSA.Δgro.UAS/Scer\GAL4h-H10

        (Kahali et al., 2009)

        Nspl-1 has scutellar bristle phenotype, non-suppressible by E(spl)m8-HLHSA.Δgro.UAS/Scer\GAL4h-H10

        (Kahali et al., 2009)

        Nspl-1 has eye phenotype, non-suppressible by E(spl)m8-HLHSD.Δgro.UAS/Scer\GAL4GMR.PU

        (Kahali et al., 2009)

        Nspl-1 has eye phenotype, non-suppressible by E(spl)m8-HLHΔgro.UAS/Scer\GAL4sca-109-68

        (Kahali et al., 2009)

        Nspl-1 has eye phenotype, non-suppressible by E(spl)m8-HLHSA.Δgro.UAS/Scer\GAL4sca-109-68

        (Kahali et al., 2009)

        Nspl-1 has scutellar bristle | ectopic phenotype, non-suppressible by Df(3R)Espl22/+

        (Kahali et al., 2009)

        Nspl-1 has scutellar bristle | ectopic phenotype, non-suppressible by Scer\GAL4h-H10/CkIIαdsRNA.UAS

        (Kahali et al., 2009)

        Nspl-1 has scutellar bristle | ectopic phenotype, non-suppressible by E(spl)m8-HLH1.UAS/Scer\GAL4h-H10

        (Kahali et al., 2009)

        Nspl-1 has scutellar bristle phenotype, non-suppressible by E(spl)m8-HLH1.UAS/Scer\GAL4h-H10

        (Kahali et al., 2009)

        Nspl-1 has eye phenotype, non-suppressible by E(spl)m8-HLHΔgro.UAS/Scer\GAL4h-H10

        (Kahali et al., 2009)

        Nspl-1 has scutellar bristle | ectopic phenotype, non-suppressible by E(spl)m8-HLHΔgro.UAS/Scer\GAL4h-H10

        (Kahali et al., 2009)

        Nspl-1 has scutellar bristle phenotype, non-suppressible by E(spl)m8-HLHΔgro.UAS/Scer\GAL4h-H10

        (Kahali et al., 2009)

        Nspl-1 has eye | male phenotype, non-suppressible by Scer\GAL4sca-109-68/Dl::SerDl-E.Ser-I.UAS

        (Li and Baker, 2004)

        Nspl-1 has photoreceptor cell R8 & eye disc | male phenotype, non-suppressible by Scer\GAL4sca-109-68/Dl::SerDl-E.Ser-I.UAS

        (Li and Baker, 2004)

        Nspl-1 has ommatidium & eye disc | male phenotype, non-suppressible by Scer\GAL4sca-109-68/Dl::SerDl-E.Ser-I.UAS

        (Li and Baker, 2004)

        Nspl-1 has photoreceptor cell & eye disc | male phenotype, non-suppressible by Scer\GAL4sca-109-68/Dl::SerDl-E.Ser-I.UAS

        (Li and Baker, 2004)

        Nspl-1 has phenotype, non-suppressible by Chie5.5

        (Rollins et al., 1999)

        Nspl-1 has phenotype, non-suppressible by Nipped-A222.3

        (Rollins et al., 1999)

        Nspl-1 has phenotype, non-suppressible by Su(H)8

        (Rollins et al., 1999)

        Nspl-1 has phenotype, non-suppressible by vg1

        (Rollins et al., 1999)

        Nspl-1 has phenotype, non-suppressible by Nipped-A394.2

        (Rollins et al., 1999)

        Nspl-1 has macrochaeta phenotype, non-suppressible by Nipped-B292.1

        (Rollins et al., 1999)

        Nspl-1 has macrochaeta phenotype, non-suppressible by Su(H)16

        (Rollins et al., 1999)

        E(spl)[+]/E(spl)m8-HLH1, Nspl-1 has eye phenotype, non-suppressible by DlHD82/Dl[+]

        (Shepard et al., 1989)

        Nspl-1 has phenotype, non-suppressible by su(Hw)2

        (Lindsley and Grell, 1968)
        Enhancer of
        Statement
        Reference

        Nspl-1 is an enhancer of mechanosensory sensory organ phenotype of Hsap\APLP2::Hsap\APPUAS.Tag:MYC, Scer\GAL4sca-537.4

        (Merdes et al., 2004)

        Nspl-1 is an enhancer of phenotype of gl3

        (Ma et al., 1996)
        Suppressor of
        Statement
        Reference

        N+/Nspl-1 is a suppressor | partially of wing blade posterior compartment | heat sensitive phenotype of Dcr-2UAS.cDa, EogtGD5084, Scer\GAL4en.PU

        (Müller et al., 2013)
        Other
        Additional Comments
        Genetic Interactions
        Statement
        Reference

        The wing blistering phenotype seen in the posterior compartment of wings in flies expressing EogtGD5084 under the control of Scer\GAL4en.PU in the presence of Dcr-2Scer\UAS.cDa is dominantly partially suppressed if the flies are also heterozygous for Nspl-1.

        (Müller et al., 2013)

        wgl-17/+ suppresses the adult eye size phenotype seen in Nspl-1 mutants.

        wgl-17/+ suppresses the cone cell and photoreceptor loss phenotypes seen in Nspl-1 third instar larvae.

        (Cordero and Cagan, 2010)

        Nspl-1/Y; E(spl)1/+ mutants exhibit a severely reduced eye. The residual eye field is highly disorganised; the clustering of the interommatidial bristles might well reflect facet loss.

        The severe reduced eye found in Nspl-1/Y; E(spl)1/+ mutants is partially suppressed upon overexpression of E(spl)123-179.CtD.Scer\UAS immediately anterior to the morphogenetic furrow under the control of Scer\GAL4h-H10. Expression of E(spl)123-179.CtD.Scer\UAS not only increases eye size (facet numbers), but also restores the patterning of the facets. This patterning is, however, lost toward the anterior margin of the residual eye.

        The severe reduced eye found in Nspl-1/Y; E(spl)1/+ mutants is unaffected by overexpression of E(spl)S159D.123-179.CtD.Scer\UAS under the control of Scer\GAL4h-H10.

        Expression of E(spl)123-179.CtD.Scer\UAS in trans, in all cells posterior to the morphogenetic furrow, under the control of Scer\GAL4GMR.PF does not rescue the severe reduced eye phenotype found in Nspl-1/Y; E(spl)1/+ mutants. The absence of rescue is also supported by facet numbers, which upon expression of three independent E(spl)123-179.CtD.Scer\UAS lines closely mimicks control levels in Nspl-1/Y; E(spl)1/+ mutants. Rescue of the Nspl-1/Y; E(spl)1/+ retinal defects by E(spl)123-179.CtD.Scer\UAS therefore requires expression in a region of the developing eye where funding R8 photoreceptors are patterned and specified.

        (Kahali et al., 2010)

        Expression of E(spl)m8-HLH1.Scer\UAS mediated by Scer\GAL4h-H10 in a Nspl-1/Y background elicits a severely reduced eye.

        Expression of E(spl)m8-HLHSD.Δgro.Scer\UAS mediated by Scer\GAL4h-H10 suppresses the Nspl-1/Y rough and reduced eye and appears to restore ommatidial phasing.

        Expression of E(spl)m8-HLHSD.Δgro.Scer\UAS mediated by Scer\GAL4sca-109-68 suppresses the Nspl-1/Y rough and reduced eye.

        Df(3R)Espl22/+ suppresses the Nspl-1/Y rough and reduced eye.

        Expression of CkIIαdsRNA.Scer\UAS or CkIIαScer\UAS.Tik mediated by Scer\GAL4h-H10 suppresses the Nspl-1/Y rough and reduced eye.

        Expression of E(spl)m8-HLHSD.Δgro.Scer\UAS mediated by Scer\GAL4h-H10 potently suppresses the Nspl-1/Y scutellar bristle phenotypes.

        Df(3R)Espl22/+ or expression of CkIIαdsRNA.Scer\UAS mediated by Scer\GAL4h-H10 suppresses the Nspl-1/Y split/missing scutellar bristle phenotype but does not modulate the Nspl-1/Y ectopic scutellar bristle phenotype.

        (Kahali et al., 2009)

        The lack of scutellar bristles seen in Nspl-1 flies is enhanced by vtd80Fh-36/+ or vtdγ26-6/+.

        (Schaaf et al., 2009)

        Expression of E(spl)1.Scer\UAS under the control of Scer\GAL4h-H10 in a Nspl-1/Y background results in reduced eyes that are similar in appearance to those seen in Nspl-1/Y single mutants.

        (Bose et al., 2006)

        E(spl)1/+, Nspl-1/Y flies have severely reduced eyes. They typically exhibit 4-8 residual ommatidia.

        (Karandikar et al., 2004)

        Expression of Dl::SerDl-E.Ser-I.Scer\UAS (line A10, P{UAS-Dl::Ser.L}A10) in R8 cells (using Scer\GAL4sca-109-68) does not rescue the adult eye or eye imaginal disc defects of hemizygous Nspl-1 males.

        (Li and Baker, 2004)

        Nspl-1/Y, Gp150k11120b/+ animals have eyes that are almost wild-type, only mild aberrations in facet arrangement and some bristle duplications remain.

        (Li et al., 2003)

        The addition of BacA\p35Scer\UAS.cHa driven by BacA\p35Scer\UAS.cHa to Nspl-1 somatic clones does not rescue all the eye phenotypes seen in Nspl-1 cells. When DlScer\UAS.cLa is driven by Scer\GAL4GMR.PF in a Nspl-1 background photoreceptor R8 differentiation is greatly reduced. The R8 photoreceptor cell phenotype seen in Nspl-1 clones is slightly enhanced by the addition of fng13.

        (Li et al., 2003)

        When DlScer\UAS.cLa is driven by Scer\GAL4GMR.PF in a Nspl-1 background photoreceptor R8 differentiation is greatly reduced.

        The R8 photoreceptor cell phenotype seen in Nspl-1 clones is slightly enhanced by the addition of fng13.

        (Li et al., 2003)

        The number of microchaetae in Nspl-1 flies is increased to 153.33 +/- 1.42 by expression of dshhs.PA using a heat pulse.

        (Ramain et al., 2001)

        The Nspl-1 eye phenotype is enhanced by E(spl)1 and further enhanced by da5 and ato3 and ato5, and suppressed by scaunspecified.

        (Nagel and Preiss, 1999)

        The addition of E(spl)1.Scer\UAS driven by Scer\GAL4unspecified to a Nspl-1 background produces a strong enhancement of the Nspl-1 eye phenotype, whilst the addition of E(spl)Scer\UAS.cGa shows little or no effect.

        (Nagel et al., 1999)

        Nipped-A222.3, Nipped-A394.2, l(2)41Ae7, Chie5.5, vg1 and Su(H)8 have little or no effect on the eye phenotype. Nipped-A222.3, Nipped-B292.1, Chie5.5, vg1, Su(H)16 and Su(H)8 have little or no effect on the bristle phenotype. Df(2R)Nipped-D341.1, Df(2R)Nipped-D263.3 and Df(2R)Nipped-E338 dominantly suppress the eye phenotype. Df(2R)Nipped-D341.1, Df(2R)Nipped-D263.3, Df(2R)Nipped-E43 and Df(2R)Nipped-E338 dominantly enhance the bristle phenotype.

        (Rollins et al., 1999)

        The heterozygous eye phenotype is unaffected if the flies are also carrying two copies of E(spl)tLa, but is enhanced if the flies are carrying two copies of E(spl)K:CAACdel or E(spl)K1K2mut; resulting in ommatidial fusion and bristle multiplication.

        (Lai et al., 1998)

        The number of thoracic bristles is increased further in hemizygous or Df(3R)Dl-FX3 ; Nspl-1 double heterozygous flies, and the number of bristles is restored to wild-type in Nspl-1; wgS107 double heterozygotes.

        (Brennan et al., 1997)

        Double mutants with cnomis1 always have rumpled wings curved downwards and extra bristles.

        (Miyamoto et al., 1995)

        Complex interactions with dx alleles.

        (Gorman and Girton, 1992)

        Nspl-1 flies also heterozygous for E(spl)1 show a conspicuous enhancement of this phenotype; in particular the size of the compound eye is severely reduced and only 15-30 widely spaced facets can be distinguished. The ommatidia contain fewer photoreceptors than normal, and the rhabdomeres are often distorted. A large number of secondary pigment cells fill the space between the scattered photoreceptor cell clusters. Photoreceptor cell axons follow an aberrant course, and usually fail to enter the eye stalk. The pattern of cell proliferation is qualitatively normal in Nspl-1/Y E(spl)1/+ flies as assayed by BrdU incorporation. There is abundant cell death in the eye imaginal discs of these flies during the third instar larval stage, dead cells are more abundant behind the morphogenetic furrow.

        (Campos-Ortega and Knust, 1990)

        Both the eye and bristle phenotype are suppressed when in combination with one copy of mam10 or mam88-4, weak suppression is seen with mam88-10. Hemizygotes and homozygotes, in combination with one copy of groE73, display an Ax-like phenotype, small gaps in the posterior end of the fifth longitudinal wing vein.

        (Xu et al., 1990)

        parX1122 Nspl-1/FM3 heterozygotes have a Nspl-1 phenotype.

        (Thierry-Mieg, 1982)

        Males carrying Nspl-1 and sno1 have difficulty in eclosion and survive only briefly.

        (Lefevre and Peterson, 1972)

        E(S)1 has little or no effect on the rough eye phenotype in ecunspecified E(S)1 double heterozygotes.

        (Lewis, 1945)
        Xenogenetic Interactions
        Statement
        Reference

        The addition of BacA\p35Scer\UAS.cHa driven by BacA\p35Scer\UAS.cHa to Nspl-1 somatic clones does not rescue all the eye phenotypes seen in Nspl-1 cells.

        (Li et al., 2003)
        Complementation and Rescue Data
        Images (1)
        Stocks (46)
        Notes on Origin
        Discoverer
        Comments
        Comments

        Nspl-1 acts as a loss of function allele with respect to N inductive processes during eye development.

        (Nagel and Preiss, 1999)
        External Crossreferences and Linkouts ( 0 )
        Synonyms and Secondary IDs (9)
        Reported As
        Symbol Synonym
        NSpl-1
        (Müller et al., 2013)
        Nsp1
        (Nagel and Preiss, 1999, Zipursky et al., 1992)
        Nspl-1
        (Dutta et al., 2021, Morimoto et al., 2016, Seugnet et al., 2011, Tsubota et al., 2011, Cordero and Cagan, 2010, Popodi et al., 2010-, Ozdowski et al., 2009, Schaaf et al., 2009)
        Nspl1
        (Dutta et al., 2021)
        Nspl
        (Majot and Bidwai, 2017, Kahali et al., 2010, Kahali et al., 2009, Bose et al., 2006, Jones et al., 2006, Tang and Sun, 2002, Indrasamy et al., 2001, Indrasamy et al., 2000, Lai et al., 1998, Brennan et al., 1997, Mackay and Fry, 1996, Ma et al., 1996, Signorelli et al., 1995, Dickson and Hafen, 1993, Woods and Bryant, 1992, Baker et al., 1990)
        Notchspl-1
        (Copeland et al., 2007)
        spl
        (Li and Baker, 2004, Li et al., 2003, Li et al., 2003, Ramain et al., 2001, Lloyd, 2000, Hing et al., 1999, Nagel et al., 1999, Rollins et al., 1999, Wesley, 1999, de Celis and Garcia-Bellido, 1994, de Celis et al., 1993, Baker and Rubin, 1992, Duus et al., 1992, Gorman and Girton, 1992, Lieber et al., 1992, Brand and Campos-Ortega, 1990, Campos-Ortega and Knust, 1990, Held, 1990, Rabinow and Birchler, 1990, Xu and Artavanis-Tsakonas, 1990, Xu et al., 1990, Alton et al., 1989, Ramos et al., 1989, Shepard et al., 1989, Knust et al., 1987, Grimwade et al., 1985, Thierry-Mieg, 1982, Shellenbarger and Mohler, 1975, Lefevre, 1974, Foster, 1973, Lefevre and Peterson, 1972, Hayman and Maddern, 1969, Welshons, 1965, Cicak and Oster, 1957, Slizynska, 1938)
        spl1
        (Heitzler and Simpson, 1993, Lindsley and Zimm, 1992, de Celis et al., 1991, Mari-Beffa et al., 1991, Renfranz and Benzer, 1989)
        split
        (Cordero and Cagan, 2010)
        Name Synonyms
        split
        (Li and Baker, 2004, Wesley and Saez, 2000, Hiebert and Birchler, 1994, Meinertzhagen and Hanson, 1993, Basler and Hafen, 1991, Hafen and Basler, 1991, Harris, 1991, Cagan and Ready, 1989, Meyer, 1952, Lewis, 1945)
        Secondary FlyBase IDs
          References (93)