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General Information
Symbol
Dmel\ninaE7
Species
D. melanogaster
Name
FlyBase ID
FBal0013012
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
ninaEP332
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

G19887586M

Amino acid change:

G128R | ninaE-PA

Reported amino acid change:
Reported amino acid change:

ninaE[7]

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: G128R.

Missense mutation in the third transmembrane domain.

normal transcript level; Gly128 --> Arg

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Electroretinograms of ninaE7 mutants do not show the expected prolonged depolarization afterpotential to blue light stimuli normally observed in controls.

ninaE7 mutant adult males have reduced ability to orient towards and remain close to a female, compared to controls; they also lose the bias seen in controls to extend the wing closest to, rather than furthest from, the female; following of females, wing extensions and copulation are significantly reduced, compared to controls.

Mutant larvae show strong discrimination between 18[o]C and 24[o]C in a binary choice thermotaxis assay (as do wild-type controls), but discrimination between 18[o]C and either 20 or 22[o]C is eliminated in the mutant larvae.

Individuals develop reasonably normal rhabdomeres and none show membrane curtains.

Reduction in the photopigment content and a similar reduction in sensitivity to light. At maximal excitation intensity the calcium signal has a similar wave form to wild type photoreceptors. A partial recovery of sensitivity of the calcium signal to a second light flask is observed.

Degeneration of photoreceptors but light-dependent 35S-labelled guanosine 5'-3-0-(thio)triphosphate (35S GTPĪ³S) binding is normal.

More rapid rate of R1-6 photoreceptor degeneration than ninaE5, but less rapid than ninaE8.

0.08% of wild-type levels of functional rhodopsin.

Retains regular rhabdomeric membranes.

Rhabdomeres of young flies are reduced in size and show aberrant packing of microvilli. Rhabdomeres are nearly wild type in adults.

The electroretinogram (ERG) of homozygous flies is abnormal; they display neither inactivation nor afterpotential, and the on-transient component is absent. Heterozygotes have an intermediate phenotype that varies somewhat with age. Homozygotes have almost no rhodopsin, heterozygotes have approximately 37% rhodopsin compared to wild-type levels. The R1-R6 rhabdomeres have normal morphology in homozygotes, although they are sightly reduced in volume.

<2% normal RH1 level

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Other
Statement
Reference
Phenotype Manifest In
Suppressor of
Statement
Reference

ninaE7 is a suppressor | partially of retina | adult stage phenotype of Culde01982

ninaE7 is a suppressor of retina phenotype of Tsp42Ej1

ninaE7 is a suppressor of phenotype of norpA36

Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

The progressive light-dependent retinal degeneration (loss of photoreceptor cells and rhabdomeres) characteristic for Culde01982 mutant adults is suppressed by combination with ninaE7. All seven rhabdomeres are still present in 15-day-old Culde01982;ninaE7 flies kept under light-dark cycle, although the rhabdomeres are smaller than in wild-type.

The termination responses of Camtates-2; ninaE7 double mutant flies are as fast as wild-type flies, but the amplitude of the responses is decreased. In Camtates-2; ninaE7/+ flies, the response amplitude is similar to wild-type, but the termination response is increased compared to Camtates-2 single mutant flies.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer

Pak.

Comments
Comments

Encodes 0.1% of wild type levels of R1-6 rhodopsin (Johnson and Pak, J. Gen Physiol. 88:651--673 ).

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
References (25)