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General Information
Symbol
Dmel\ninaE8
Species
D. melanogaster
Name
FlyBase ID
FBal0013013
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
ninaEP334
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

C19886887T

Amino acid change:

T283M | ninaE-PA

Reported amino acid change:
Reported amino acid change:

T283M

Comment:

One of three mutations associated with this allele; Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Nucleotide change:

T19886870C

Amino acid change:

W289R | ninaE-PA

Reported amino acid change:
Reported amino acid change:

W289R

Comment:

One of three mutations associated with this allele; G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation (exact site of mutation unspecified). Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Nucleotide change:

T19886846A

Amino acid change:

C297S | ninaE-PA

Reported amino acid change:

C297S

Comment:

Amino acid replacement reported as reported as Cys to Ser, which could result from a nucleotide substitution in the first or second base of the codon. The mutation was annotated at the first base of the codon. One of three amino acid changes observed in ninaE in mutant strain.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: T283M. Amino acid replacement: W289R. Amino acid replacement: C297S. Mutations lie within the sixth transmembrane domain.

Amino acid replacement: T283M. Amino acid replacement: W239R. Amino acid replacement: C297S.

normal transcript level; Thr283 --> Met; Trp289 --> Arg; Cys297 --> Ser

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Electroretinograms from ninaE8 flies fail to show prolonged depolarization afterpotential (PDA) in response to blue light.

Homozygous flies and mosaic flies in which the retinal tissue is homozygous for ninaE8 but the rest of the animal is ninaE+ lack the prolonged depolarising afterpotential (PDA) in the electroretinogram response.

ninaE8 larvae reduce their path lengths and show increased head swinging when exposed to light. This response is indistinguishable from wild-type larvae. Wild-type larvae show a greater change in direction when lights are turned on or off (light (L) to dark (D), or D to L transition) than in the absence of a light transition (D to D). The amplitude of change of direction is greater for the D to L than for the L to D transition. The responses are wild type in ninaE8 larvae.

Individuals develop reasonably normal rhabdomeres and none show membrane curtains.

More rapid rate of R1-6 photoreceptor degeneration than ninaE7, but less rapid than ninaE9.

ERG phenotype: light coincident response is small, transients are absent. Rhodopsin levels very low in R1--R6 photoreceptors: respond poorly or not at all to light stimulus.

The electroretinogram (ERG) of homozygous flies is abnormal; the on- and off- transient components are absent. Homozygotes have almost no rhodopsin, heterozygotes have approximately 57% rhodopsin compared to wild-type levels.

<1% normal RH1 level

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressor of
Statement
Reference

ninaE8 is a suppressor of phenotype of norpA36

Additional Comments
Genetic Interactions
Statement
Reference

The deactivation of the electroretinogram photoresponse is much faster than in the Gα49B961 mutant in Gα49B961 ninaE8 double mutant flies.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer

Pak.

Comments
Comments

Encodes 4x10-4% of wild type levels of R1-6 rhodopsin (Johnson and Pak, J. Gen Physiol. 88:651--673 ).

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
References (18)