FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\ninaE8
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General Information
Symbol
Dmel\ninaE8
Species
D. melanogaster
Name
FlyBase ID
FBal0013013
Feature type
allele
Associated gene
Dmel\ninaE
Associated Insertion(s)
Carried in Construct
Also Known As
ninaEP334
Key Links
Genomic Maps

Allele class

hypomorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

hypomorphic allele - genetic evidence

(Kumar and Ready, 1995)
Mutagen
ethyl methanesulfonate
Progenitor genotype
Cytology
Description

Amino acid replacement: T283M. Amino acid replacement: W289R. Amino acid replacement: C297S. Mutations lie within the sixth transmembrane domain.

(Kumar and Ready, 1995)

Amino acid replacement: T283M. Amino acid replacement: W239R. Amino acid replacement: C297S.

(Kurada and O'Tousa, 1995)

normal transcript level; Thr283 --> Met; Trp289 --> Arg; Cys297 --> Ser

Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3R:19,886,887..19,886,887 [-]
Nucleotide change:

C19886887T

Amino acid change:

T283M | ninaE-PA

Reported amino acid change:

T283M|FBrf0080181

Reported amino acid change:

T283M

Comment:

One of three mutations associated with this allele; Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Kumar and Ready, 1995, Kurada and O'Tousa, 1995)
point_mutation
3R:19,886,870..19,886,870 [-]
Nucleotide change:

T19886870C

Amino acid change:

W289R | ninaE-PA

Reported amino acid change:

W289R|FBrf0080181

Reported amino acid change:

W289R

Comment:

One of three mutations associated with this allele; G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation (exact site of mutation unspecified). Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Kumar and Ready, 1995, Kurada and O'Tousa, 1995)
point_mutation
3R:19,886,846..19,886,846 [-]
Nucleotide change:

T19886846A

Amino acid change:

C297S | ninaE-PA

Reported amino acid change:

C297S

Comment:

Amino acid replacement reported as reported as Cys to Ser, which could result from a nucleotide substitution in the first or second base of the codon. The mutation was annotated at the first base of the codon. One of three amino acid changes observed in ninaE in mutant strain.

(Kumar and Ready, 1995, Kurada and O'Tousa, 1995)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Electroretinograms from ninaE8 flies fail to show prolonged depolarization afterpotential (PDA) in response to blue light.

      (Wang and Montell, 2005)

      Homozygous flies and mosaic flies in which the retinal tissue is homozygous for ninaE8 but the rest of the animal is ninaE+ lack the prolonged depolarising afterpotential (PDA) in the electroretinogram response.

      (Gu et al., 2004)

      ninaE8 larvae reduce their path lengths and show increased head swinging when exposed to light. This response is indistinguishable from wild-type larvae. Wild-type larvae show a greater change in direction when lights are turned on or off (light (L) to dark (D), or D to L transition) than in the absence of a light transition (D to D). The amplitude of change of direction is greater for the D to L than for the L to D transition. The responses are wild type in ninaE8 larvae.

      (Busto et al., 1999)

      Individuals develop reasonably normal rhabdomeres and none show membrane curtains.

      (Kumar and Ready, 1995)

      More rapid rate of R1-6 photoreceptor degeneration than ninaE7, but less rapid than ninaE9.

      (Leonard et al., 1992)

      ERG phenotype: light coincident response is small, transients are absent. Rhodopsin levels very low in R1--R6 photoreceptors: respond poorly or not at all to light stimulus.

      (O'Tousa et al., 1989)

      The electroretinogram (ERG) of homozygous flies is abnormal; the on- and off- transient components are absent. Homozygotes have almost no rhodopsin, heterozygotes have approximately 57% rhodopsin compared to wild-type levels.

      (Scavarda et al., 1983)

      <1% normal RH1 level

      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressor of
      Statement
      Reference

      ninaE8 is a suppressor of phenotype of norpAP24

      (Kurada and O'Tousa, 1995)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The deactivation of the electroretinogram photoresponse is much faster than in the Gα49B961 mutant in Gα49B961 ninaE8 double mutant flies.

      (Hu et al., 2012)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer

      Pak.

      Comments
      Comments

      Encodes 4x10-4% of wild type levels of R1-6 rhodopsin (Johnson and Pak, J. Gen Physiol. 88:651--673 ).

      (Leonard et al., 1992)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      References (19)