ph-p409 embryos from homozygous or heterozygous mothers exhibit segregation defects at anaphase and telophase. Late anaphase nuclei are frequently connected by chromatin bridges; these bridges can be thick which make the joined nuclei an irregular size, or be thin and appear as threadlike chromatin connections between sets of sister chromatids. These chromatin bridges persist to telophase. Most of these chromatin bridges are probably resolved because large irregularly-shaped nuclei and polyploid nuclei are only seen at a low penetrance.
ph-p409 embryos show a higher level of "nuclear fallout", a process that removes nuclei with abnormal mitoses, than wild-type embryos. Fallout nuclei tend to be observed in pairs or clusters and have a late telophase appearance. There is no evidence of metaphase defects in these embryos although a small number show chromatin bridges that persist to the prometaphase stage. 2% of these embryos show a "mitotic catastrophe" phenotype, characterized by completely disorganized chromosomes that have no regular pattern or normal ploidy and very few, abnormally large nuclei that may be amorphous or may have a normal, spherical shape.
ph-p409/+ heterozygous mutant flies exhibit a higher than wild-type average number of legs with sex combs, with an average of 2.7 legs with sex combs, compared to 2.0 in wild-type, although this is not statistically significant.
ph-p409/Df(3R)ry27 mutant flies exhibit a higher than wild-type average number of legs with sex combs, with a statistically significant average of 3.6 legs with sex combs, compared to 2.8 in ph-p409/+ heterozygotes.
ph-p409/Df(3R)ry619 mutant flies exhibit a higher than wild-type average number of legs with sex combs, with a statistically significant average of 5.1 legs with sex combs, compared to 4.1 in ph-p409/Tp(3;3)MRS heterozygotes.
Mutation changes the level of w expression in ph-plac+3 flies; eye colour is darker.
Viable when hemizygous and homozygous.