Open Close
General Information
Symbol
Dmel\ph-p409
Species
D. melanogaster
Name
FlyBase ID
FBal0013767
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
ph409
Key Links
Mutagen
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology

Polytene chromosomes normal? Chromosome mutant for ph and wapl but not for the intervening Pgd; probably carries an inversion with breaks in ph and wapl (Dura et al., 1987).

Nature of the lesion
Statement
Reference

Inversion breakpoint in ph-p.

Breakpoint within ph-p.

Mutation in ph-p.

Inversion breaks ph-p and wapl.

Deletion in the proximal repeat.

Caused by aberration
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

ph-p409 embryos from homozygous or heterozygous mothers exhibit segregation defects at anaphase and telophase. Late anaphase nuclei are frequently connected by chromatin bridges; these bridges can be thick which make the joined nuclei an irregular size, or be thin and appear as threadlike chromatin connections between sets of sister chromatids. These chromatin bridges persist to telophase. Most of these chromatin bridges are probably resolved because large irregularly-shaped nuclei and polyploid nuclei are only seen at a low penetrance.

ph-p409 embryos show a higher level of "nuclear fallout", a process that removes nuclei with abnormal mitoses, than wild-type embryos. Fallout nuclei tend to be observed in pairs or clusters and have a late telophase appearance. There is no evidence of metaphase defects in these embryos although a small number show chromatin bridges that persist to the prometaphase stage. 2% of these embryos show a "mitotic catastrophe" phenotype, characterized by completely disorganized chromosomes that have no regular pattern or normal ploidy and very few, abnormally large nuclei that may be amorphous or may have a normal, spherical shape.

ph-p409/+ heterozygous mutant flies exhibit a higher than wild-type average number of legs with sex combs, with an average of 2.7 legs with sex combs, compared to 2.0 in wild-type, although this is not statistically significant.

ph-p409/Df(3R)ry27 mutant flies exhibit a higher than wild-type average number of legs with sex combs, with a statistically significant average of 3.6 legs with sex combs, compared to 2.8 in ph-p409/+ heterozygotes.

ph-p409/Df(3R)ry619 mutant flies exhibit a higher than wild-type average number of legs with sex combs, with a statistically significant average of 5.1 legs with sex combs, compared to 4.1 in ph-p409/Tp(3;3)MRS heterozygotes.

Mutation changes the level of w expression in ph-plac+3 flies; eye colour is darker.

Viable when hemizygous and homozygous.

Adults viable.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Other
Phenotype Manifest In
Enhanced by
Statement
Reference

ph-p409 has sex comb phenotype, enhanceable by Hsc70-4195/Hsc70-4[+]

Suppressor of
Additional Comments
Genetic Interactions
Statement
Reference

ph-p409/Hsc70-4195 mutant flies exhibit a higher than wild-type average number of legs with sex combs, with a statistically significant average of 4.8 legs with sex combs, compared to 2.7 in ph-p409/+ heterozygotes.

ph-p409 completely suppresses the strong silencing of wBR.E.BR in P{SUPor-P}22F caused by tethered Pc, and substantially suppresses the strong silencing of wBR.E.BR in P{SUPor-P}22F caused by tethered Scm or esc.

ph-p409 males that are also heterozygous for ScmM56, ScmD1, ScmSuz302, ScmM36, Scm4, ScmD2, ScmH1, ScmET50, ScmR5-13, Scm5 or ScmK2 show partial lethality.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
References (14)