FlyBase Allele Report: Dmel\pip[1]

General Information

Symbol

Dmel\pip¹

Species

D. melanogaster

Name

pipe¹

FlyBase ID

FBal0013836

Feature type

allele

Associated gene

Dmel\pip

Associated Insertion(s)

Carried in Construct

Also Known As

pip³⁸⁶, pipe³⁸⁶, pipe¹

Genomic Maps

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Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Tearle and Nusslein-Volhard, 1987)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

point mutation

3L:19,300,115..19,300,115 [-]

Nucleotide change:

T19300115A

Amino acid change:

V133D | pip-PA

Reported amino acid change:

V123D

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. Difference between reported and FlyBase amino acid change due to authors use of GenBank record gi:7293803 which has since been replaced by a new version gi:28380469 .

(Zhu et al., 2005)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Cytology

Nature of the lesion

Statement

Reference

Mutation is within the fourth pip-PA (Pipe-ST2) specific exon.

(Zhu et al., 2005)

Amino acid replacement: V123D.

(Zhu et al., 2005)

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/first instar larval cuticle

(Sen et al., 1998)

embryonic/first instar larval cuticle | maternal effect

(Zhu et al., 2005)

embryonic epidermis | ventral

(Nilson and Schupbach, 1998)

Detailed Description

Statement

Reference

pip¹ homozygous females produce dorsalised embryos.

(Shapiro and Anderson, 2006)

All embryos from females carrying pip¹ are completely dorsalized.

(Zhu et al., 2005)

Eggs from pip¹/pip² mothers do not have apparent vitelline membrane defects.

(LeMosy and Hashimoto, 2000)

Dorsalised embryos. dec-1-marked clones restricted to the dorsal side of the follicular epithelium produce normal embryos with no defects in dorsoventral patterning. Clones on the dorsal side produce local dorsalisation of the embryo. Clones in the ventral anterior region yield embryos exhibiting anterior dorsalisation, clones in the ventral posterior produce result in dorsalisation of the embryo at the posterior.

(Nilson and Schupbach, 1998)

Embryos derived from pip¹/pip² females are dorsalised, showing symmetric folds during gastrulation and forming a tube of dorsal cuticle. Embryos derived from pip¹/pip² females expressing pip^ST2.Scer\UAS under the control of Scer\GAL4²⁵ show strong ventralisation, producing cuticles with rings of ventral denticle material. The orientation of gastrulation in these embryos is variable and they show muscle movement. Embryos derived from pip¹/pip² females expressing pip^ST2.Scer\UAS under the control of Scer\GAL4²⁴¹ show gastrulation movements that are inverted with respect to the intrinsic orientation of the eggshell. The most ventral structures differentiated by most of these embryos are Filzkorper, although some embryos develop ventral denticle material. Embryos derived from pip¹/pip² females expressing pip^ST2.Scer\UAS under the control of Scer\GAL4^DE1 form a ventral furrow invagination only at the posterior end. These embryos form rings of denticles at the posterior end.

(Sen et al., 1998)

Mutant embryos respond to injected purified polarizing activity (spz), and produce polarizing activity themselves.

(Schneider et al., 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

pip¹ has embryo | dorsal phenotype, suppressible by gd^WT.cHa

(Han et al., 2000)

Other

Statement

Reference

Scer\GAL4^unspecified, ea^UASp.Tag:HA, pip³/pip¹, snk^UASp.mGFP6 has embryonic/first instar larval cuticle | maternal effect phenotype

(Cho et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of ea^{Scer\UAS.P\T.T:Ivir\HA1} and snk^{Scer\UAS.P\T.T:Avic\GFP-m6} using Scer\GAL4^unspecified in a pip¹/pip³ background results in the production of dorsalized embryos.

(Cho et al., 2010)

pip¹/pip¹ and pip¹/pip² mutants carrying germ-line clones of ik2¹ mothers produce dorsalised embryos, in the same manner as homozygous pip¹ or pip¹/pip² mutants.

(Shapiro and Anderson, 2006)

Embryos derived from Spn27A^ex32/Df(2L)BSC7 ; pip¹/pip² females are completely dorsalised.

(Ligoxygakis et al., 2003)

Perivitelline fluid from embryos laid by spz⁴/Df(3R)Bd females does not show axis-inducing activity when injected into the perivitelline space of embryos derived from pip¹/pip² females. Perivitelline fluid from embryos laid by spz^D1-RPQ/Df(3R)Bd females does not show axis-inducing activity when injected into the perivitelline space of embryos derived from pip¹/pip² females. Perivitelline fluid from embryos laid by Tl^rv13 spz⁴/Tl^rv4 spz² females does not show axis-inducing activity when injected into the perivitelline space of embryos derived from pip¹/pip² females. Perivitelline fluid from embryos laid by ea^D3 spz⁴/ea¹ spz² females does not show axis-inducing activity when injected into the perivitelline space of embryos derived from pip¹/pip² females. Perivitelline fluid from embryos laid by ea^D1 spz²/ea⁴ spz⁴ females shows axis-inducing activity when injected into the the perivitelline space of embryos derived from pip¹/pip² females. Perivitelline fluid from embryos laid by Tl^rv13/Tl^rv19 females shows polarising activity when injected into the perivitelline space of embryos derived from pip¹/pip² females.

(Chang and Morisato, 2002)

Double mutant combinations with ea^D1 are strongly dorsalizing.

(Chasan et al., 1992)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

pip²/pip¹ is partially rescued by pip^ST2.UAS/Scer\GAL4^DE1

(Sen et al., 1998)

pip²/pip¹ is partially rescued by Scer\GAL4²⁴¹/pip^ST2.UAS

(Sen et al., 1998)

pip²/pip¹ is partially rescued by Scer\GAL4²⁵/pip^ST2.UAS

(Sen et al., 1998)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

3110

st¹ pip¹ e¹/TM8, l(3)DTS4¹

Kyoto

106851

st¹ pip¹ e¹/TM8, l(3)DTS4¹

Notes on Origin

Discoverer

Comments

Strong mutation.

(Nilson and Schupbach, 1998)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

pip¹

(Sergeev et al., 2001)

pip³⁸⁶

(Steen et al., 2010, Zhu et al., 2005, Chang and Morisato, 2002, LeMosy et al., 2001, Han et al., 2000, LeMosy and Hashimoto, 2000, LeMosy et al., 1998, Nilson and Schupbach, 1998, Stein et al., 1998, Morisato and Anderson, 1994, Schneider et al., 1994, Chasan et al., 1992, Roth et al., 1991, Tearle and Nusslein-Volhard, 1987, Anderson et al., 1985)

pipe386

(Garcia and Stathopoulos, 2011)

pipe¹

(Cho et al., 2010, Zhang et al., 2009)

pipe³⁸⁶

(Biemar et al., 2005, Ligoxygakis et al., 2003, Stathopoulos et al., 2002, Fernandez et al., 2001, Sen et al., 1998)

Name Synonyms

pipe1

(Stein et al., 2008, LeMosy, 2006, Shapiro and Anderson, 2006)

pipe386

(Stein et al., 2008, Biemar et al., 2006)

Secondary FlyBase IDs

References (32)

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