T19300115A
V133D | pip-PA
V123D
Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. Difference between reported and FlyBase amino acid change due to authors use of GenBank record gi:7293803 which has since been replaced by a new version gi:28380469 .
Mutation is within the fourth pip-PA (Pipe-ST2) specific exon.
Amino acid replacement: V123D.
pip1 homozygous females produce dorsalised embryos.
All embryos from females carrying pip1 are completely dorsalized.
Dorsalised embryos. dec-1-marked clones restricted to the dorsal side of the follicular epithelium produce normal embryos with no defects in dorsoventral patterning. Clones on the dorsal side produce local dorsalisation of the embryo. Clones in the ventral anterior region yield embryos exhibiting anterior dorsalisation, clones in the ventral posterior produce result in dorsalisation of the embryo at the posterior.
Embryos derived from pip1/pip2 females are dorsalised, showing symmetric folds during gastrulation and forming a tube of dorsal cuticle. Embryos derived from pip1/pip2 females expressing pipST2.Scer\UAS under the control of Scer\GAL425 show strong ventralisation, producing cuticles with rings of ventral denticle material. The orientation of gastrulation in these embryos is variable and they show muscle movement. Embryos derived from pip1/pip2 females expressing pipST2.Scer\UAS under the control of Scer\GAL4241 show gastrulation movements that are inverted with respect to the intrinsic orientation of the eggshell. The most ventral structures differentiated by most of these embryos are Filzkorper, although some embryos develop ventral denticle material. Embryos derived from pip1/pip2 females expressing pipST2.Scer\UAS under the control of Scer\GAL4DE1 form a ventral furrow invagination only at the posterior end. These embryos form rings of denticles at the posterior end.
Mutant embryos respond to injected purified polarizing activity (spz), and produce polarizing activity themselves.
Co-expression of eaScer\UAS.P\T.T:Ivir\HA1 and snkScer\UAS.P\T.T:Avic\GFP-m6 using Scer\GAL4unspecified in a pip1/pip3 background results in the production of dorsalized embryos.
Embryos derived from Spn27Aex32/Df(2L)BSC7 ; pip1/pip2 females are completely dorsalised.
Perivitelline fluid from embryos laid by spz4/Df(3R)Bd females does not show axis-inducing activity when injected into the perivitelline space of embryos derived from pip1/pip2 females. Perivitelline fluid from embryos laid by spzD1-RPQ/Df(3R)Bd females does not show axis-inducing activity when injected into the perivitelline space of embryos derived from pip1/pip2 females. Perivitelline fluid from embryos laid by Tlrv13 spz4/Tlrv4 spz2 females does not show axis-inducing activity when injected into the perivitelline space of embryos derived from pip1/pip2 females. Perivitelline fluid from embryos laid by eaD3 spz4/ea1 spz2 females does not show axis-inducing activity when injected into the perivitelline space of embryos derived from pip1/pip2 females. Perivitelline fluid from embryos laid by eaD1 spz2/ea4 spz4 females shows axis-inducing activity when injected into the the perivitelline space of embryos derived from pip1/pip2 females. Perivitelline fluid from embryos laid by Tlrv13/Tlrv19 females shows polarising activity when injected into the perivitelline space of embryos derived from pip1/pip2 females.
Double mutant combinations with eaD1 are strongly dorsalizing.
pip2/pip1 is partially rescued by pipST2.UAS/Scer\GAL4DE1
pip2/pip1 is partially rescued by Scer\GAL4241/pipST2.UAS
pip2/pip1 is partially rescued by Scer\GAL425/pipST2.UAS
Strong mutation.