meiosis II & aster | female
meiosis II & spindle | female
meiotic cell cycle & spindle | male
meiotic metaphase II & spindle | female
meiotic telophase II & contractile ring | female
neuroblast & centrosome & larva
neuroblast & mitotic anaphase & larva
spermatocyte & midbody
spermatocyte & pericentriolar material
spermatocyte & spindle
Expression of maelS138D.Scer\UAS.P\T.T:Zzzz\FLAG under the control of Scer\GAL4nos.PG partially suppresses the defect in oocyte commitment (high frequency of 'two-oocyte phenotype' in region 3 of the germarium) that is seen in polo1/poloS025604 germaria.
Expression of maelS138A.Scer\UAS.P\T.T:Zzzz\FLAG under the control of Scer\GAL4nos.PG does not suppress the defect in oocyte commitment (high frequency of 'two-oocyte phenotype' in region 3 of the germarium) that is seen in polo1/poloS025604 germaria.
Heterozygosity for either twsaar-1, twsj11C8 or mtsXE-2202 enhances the reduced fertility phenotype of polo1/+ gwlscant/+ double heterozygous females, such that in each case the triple heterozygotes are completely sterile.
polo1 gwlscant double heterozygous females produce embryos that frequently display centrosome disassociation from one pole. There is a slight but significant increase in defective spindles in embryos derived from polo1 gwlscant mutants compared to controls, indicating that a single mutant copy of these genes in mothers leads to mitotic defects at a low frequency. However, these embryos always hatch and develop fully, indicating that such low frequencies of defects can be tolerated.
The lethal phase of Cdc27S092309 is advanced to an earlier developmental stage when in combination with polo1. The stage of death is also earlier than with polo1 alone. In terms of the mitotic index, the metaphase:anaphase ratio, the condensation state of the chromosomes and the levels of mitotic cyclins, the phenotype of Cdc27S092309 polo1 cells do not differ significantly from that of the Cdc27S092309 single mutant. The polo1 phenotype with respect to centrosomal defects is enhanced by the Cdc27S092309 mutation. Mitotically arrested Cdc27S092309 polo1 cells, lack the MPM2 epitope at their spindle poles, consistent with diminished levels of polo kinase activity at the spindle poles. Cdc27S092309 is epistatic to polo1 in respect of mitotic progression and cyclin degradation. Cdc27S092309 polo1 double mutant cells display overcondensed chromosomes. In Cdc27S092309 polo1 double mutants the mitotic index, proportions of metaphase:anaphase cells and the proportion of polyploids are similar to that in the Cdc27S092309 mutant alone.
polo1 mgrunspecified double mutants show a phenotype more similar to mgrunspecified than polo1 single mutants, although the frequency of circular figures appears to be additive. aspE3 polo1 double mutants show mitotic abnormalities similar to those seen in aspE3 single mutants, including polyploid and aneuploid cells with various degrees of chromosome condensation. There are a large number of cells in metaphase, and unlike the polo1 single mutant, no normal metaphase or anaphase figures and no circular mitotic figures are seen. The mitotic frequency of the double mutant and the frequency of polyploid cells are dramatically increased compared to that of either single mutant, indicating a synergistic effect.
polo1/+ partially suppresses the wing expansion defects, shorter lifespan, locomotion defects (larval and adult), and increases of Is bouton numbers (but does not suppress branch number changes or the decrease of Ib boutons) at the larval neuromuscular junction seen in animals with Hsap\APP695.Scer\UAS driven by Scer\GAL4Appl.G1a.
polowt.T:Avic\GFP substantially rescues the sterility of polo1/Df(3L)rdgC-co2 females: in the presence of one copy of polowt.T:Avic\GFP the rescued females produce an average of 23.9 progeny per female over 18 days when mated to wild-type males and in the presence of two copies, produce an average of 30.9 progeny per female.