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General Information
Symbol
Dmel\sbr10
Species
D. melanogaster
Name
FlyBase ID
FBal0015174
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
l(1)ts403
Key Links
Mutagen
    Nature of the Allele
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    Nucleotide change:

    C10837150T

    Reported nucleotide change:

    C416T

    Amino acid change:

    P139L | sbr-PA; P3L | sbr-PB; P3L | sbr-PC

    Reported amino acid change:

    P139L

    Comment:

    In addition to the Pro to Leu change at residue 139, two conservative amino acid changes are observed in the sbr10 mutant.

    Nucleotide change:

    A10834117T

    Reported nucleotide change:

    A1477T

    Amino acid change:

    T493S | sbr-PA; T357S | sbr-PB; T357S | sbr-PC

    Reported amino acid change:

    T493S

    Comment:

    One of two conservative amino acid changes observed in the sbr10 mutant in addition to the Pro to Leu change at residue 139.

    Nucleotide change:

    G10834097T

    Reported nucleotide change:

    G1497T

    Amino acid change:

    M499I | sbr-PA; M363I | sbr-PB; M363I | sbr-PC

    Reported amino acid change:

    M499I

    Comment:

    One of two conservative amino acid changes observed in the sbr10 mutant in addition to the Pro to Leu change at residue 139.

    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference

    Nucleotide substitution: C416T. Nucleotide substitution: A1477T. Nucleotide substitution: G1497T. Amino acid replacement: P139L. Amino acid replacement: T493S. Amino acid replacement: M499I.

    Amino acid replacement: P139L.

    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease-implicated variant(s)
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In

    meiosis & nuclear chromosome

    Detailed Description
    Statement
    Reference

    Flies subjected to heat shocks have high learning indices immediately and 30 minutes after training, indicating normal learning and memory acquisition in the mutant flies. Memory retention 3 hours after training is impaired.

    Mutant females exposed to 37oC for 1 hour show a high frequency of nondisjunction and loss of sex chromosomes in meiosis. This phenotype shows incomplete dominance. Homozygous females exposed to 37oC for 1 hour show reduced viability and low fertility.

    Heat treated females show a high frequency of meiotic sex-chromosome nondisjunction.

    Under normal conditions, juvenile hormone (JH)-hydrolysing activity in 1 or 6 day old mutant females does not differ from wild type. Mutant and wild-type females exposed to 38oC show a significant decrease in JH-hydrolysing activity compared to control females at 25oC. 1 day old mutant females have lower levels of stress reactivity (calculated as the percent decrease in JH-hydrolysing activity at 38oC relative to normal conditions) than control females. 6 day old mutant females have significantly higher levels of stress reactivity than control females.

    Mutation shows no maternal effect. Mutation affects early embryonic thermosensitivity.

    Heat shock of 37oC for 1 hour during oogenesis stages 6-14 causes a high frequency of chromosome loss and non-disjunction. Successive action of anoxia and heat shock decreased the frequency of chromosome loss and non-disjunction.

    Lethal at 29oC, semi-lethal at 25oC, escapers have a small body.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Phenotype Manifest In
    Additional Comments
    Genetic Interactions
    Statement
    Reference
    Xenogenetic Interactions
    Statement
    Reference
    Complementation and Rescue Data
    Fails to complement
    Comments
    Images (0)
    Mutant
    Wild-type
    Stocks (0)
    Notes on Origin
    Discoverer
    Comments
    Comments

    After heat shock, mutant cells lack Hsp83 and Hsp35 expression, and also lack expression of some of the Hsp70 genes. A 72kD heat shock cognate protein which is not usually induced by high temperatures is intensively synthesised in mutant cells after heat shock.

    The dynamics of heat-shock puff activity and cell nuclei electrophoretic mobility in sbr10 larval salivary glands after heat shock have been studied.

    There is a delay in reaching the maximum level of heat shock protein synthesis and a more prolonged synthesis of these proteins after heat shock compared to wild-type.

    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (4)
    References (30)