lamina & neuron | precursor | somatic clone
morphogenetic furrow & nucleus | somatic clone
photoreceptor cell & neuron
photoreceptor cell R8 & eye disc | somatic clone
The defects of smo3 homozygous somatic clones in third instar larval eye discs to enter S transition during the second mitotic wave, are suppressed by the expression of gigGD1454 (and Dicer-2 transgene, for efficient RNAi) under the control of Scer\GAL4mirr-DE.
24 hours after UV-induced nociceptive sensitization, early third instar smo3/+;TkΔ1C/+ larvae show a significant reduction of thermal allodynia (withdrawal responses to a sub-threshold 38[o]C probe) compared to heterozygotic smo3/+ or TkΔ1C/+ larvae.
Expression of Cad86CScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4Act5C.PP in smo3 tkv4 double homozygous clones in the region of the morphogenetic furrow can result in the formation of an epithelial invagination in some cases. Cells at the centre of this invagination are shorter along their apicobasal axis compared to smo3 tkv4 double homozygous clones or to wild-type cells within the morphogenetic furrow.
The rapid loss of smo3 homozygous somatic clone cells from the somatic stem cell population of the germarium is not significantly suppressed if the clone cells are also tkvQ199D.Scer\UAS; Scer\GAL4Act5C.PI (Scer\GAL80 method).
Independent ed1X5, smo3 double mutant clones that originate in different compartments (as evidenced by ci or en expression) can fuse together to form composite clones which have roundish, smooth shapes. Within such composite clones, the characteristic segregation of 'anterior' and 'posterior' cells is maintained - they do not intermix.
R8 differentiation fails in tkv8; smo3 or Mad1-2; smo3 double mutant clones, whereas it is only delayed in smo3 single mutant clones. The delay in R8 photoreceptor differentiation seen in smo3 mutant somatic clones is suppressed by ci94.
Expression of high levels of fz::smoSSF.Scer\UAS under the control of Scer\GAL4Bx-MS1096 in a smo3/+ background results in a reduction in the spacing between the 3rd and 4th wing vein. Co-expression of fuScer\UAS.cAb only weakly suppresses the reduction in spacing between the 3rd and 4th wing vein seen in flies expressing high levels of fz::smoSSF.Scer\UAS under the control of Scer\GAL4Bx-MS1096 in a smo3/+ background. The reduction in spacing between the 3rd and 4th wing vein seen in flies expressing high levels of fz::smoSSF.Scer\UAS under the control of Scer\GAL4Bx-MS1096 in a smo3/+ background is suppressed by co-expression of smoScer\UAS.cHa.T:Hsap\MYC. The ectopic venation and costal overgrowth caused by expression of smoC.Scer\UAS.T:Hsap\MYC,T:Uuuu\Myr4 under the control of hemizygous Scer\GAL4Bx-MS1096 in a smo3/+ background is enhanced by co-expression of fuScer\UAS.cAb, while the L3/4 narrowing is suppressed.
Ommatidial clusters situated posterior to smo3; tkv8 double mutant clones differentiate properly, but over-rotate at a much higher frequency than those situated in other regions of the same eye disc, with many of them reaching 110o-120o.
smo3 homozygous clones generated in an enApa/+ background have no effect on cell fate or polarity in the tergites; smo3 mutant cells located posterior to the line of symmetry retain posterior orientation, while smo3 mutant cells located anterior to the line retain anterior orientation. The smo3 clones interdigitate freely with the surrounding cells.
Pka-C1E95, sggM11 smo3 triple mutant clones in the wing disc exhibit some outgrowths from the notum but no significant wing pattern duplications. Wing discs with Pka-C1E95, smo3 clones have large expansions affecting the anterior compartment of the wing disc.
smo3 Mad1-2 double mutant clones in the eye never develop photoreceptors whether they lie at the margin or in the interior of the eye disc. Occasionally an ommatidium forms along the edge of the clone, such that some of the component photoreceptors lie within the clone.
Clones in the posterior compartment of the wing disc which are expressing hhαTub84B.PB in a smo3 background form wiggly borders with the surrounding cells. Clones in the anterior compartment of the wing disc which are expressing hhαTub84B.PB in a smo3 background form significantly smoother borders with neighbouring wild-type cells. Unlike Df(2R)enE single mutant clones, Df(2R)enE smo3 double mutant clones of posterior origin in the wing disc invariably occupy only posterior territory and define straight borders to anterior cells at the normal position of the anterior/posterior (A/P) boundary. Df(2R)enE smo3 double mutant clones of anterior origin also occupy posterior territory and define straight borders to anterior cells at the normal position of the A/P boundary.
Clones mutant for smo3 and Df(2R)enE induced in the posterior compartment of the abdominal tergite make a3 or a2 cuticle. The small number of clones produced after clone formation in the embryo tend to be elliptical with smooth boundaries. Clone induction in the larva produces mutant clones with less pigment and small bristles. These cells survive better than Df(2R)enE clones, especially in the posterior part of the P compartment. The boundaries of these clones are smooth. Clones mutant for smo3 and Df(2R)enE induced in the posterior compartment of the pleura form a3 or a2 cuticle embedded in the A compartments, having been ejected from the posterior compartment.
ptcG12 homozygotes exhibit reduced number of denticle rows and each belt is rectangular shape. ptcG12 smo3 double homozygotes raised at 25oC show an essentially wild type phenotype. Scer\GAL4h-1J3-mediated expression of hhScer\UAS.cIa at 18oC does not modify the smo phenotype. Scer\GAL4h-1J3-mediated expression of wgScer\UAS.cLa at 18oC suppresses the smo denticle belt phenotype.