FlyBase Allele Report: Dmel\sv[spa-pol]

General Information

Symbol

Dmel\sv^spa-pol

Species

D. melanogaster

Name

poliert

FlyBase ID

FBal0015991

Feature type

allele

Associated gene

Dmel\sv

Associated Insertion(s)

Carried in Construct

Also Known As

spa^pol

Key Links

Allele class

Mutagen

spontaneous

Nature of the Allele

Allele class

Mutagen

spontaneous

(Fu and Noll, 1997)

Progenitor genotype

Cytology

Description

1.58kb deficiency extending from the second to the fourth intron.

(Fu et al., 1998)

1.58kb deficiency removing exons 3 and 4 and flanking sequences.

(Fu and Noll, 1997)

1.5kb deficiency deleting two small exons and flanking intron sequences.

(Fu and Noll, 1996)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female fertile (with sv^B)

(Weasner et al., 2025)

female fertile (with sv^F)

(Weasner et al., 2025)

increased cell death

(Siddall et al., 2003)

visible

(Sousa-Neves et al., 2005, Siddall et al., 2003, Jiao et al., 2001, Flores et al., 2000)

visible (with sv^B)

(Weasner et al., 2025)

visible (with sv^rugoso-nu)

(Sousa-Neves et al., 2005)

visible | recessive

(Brunner et al., 1999, Fu and Noll, 1997, Hochman et al., 1964)

Phenotype Manifest In

Detailed Description

Statement

Reference

When the majority of eye cells are homozygous sv^spa-pol mutant using Minute clones, the corneal lenses display slightly irregular shapes and sizes compared with wild-type eyes. The mutant lenses become progressively less defined posteriorly.

(Charlton-Perkins et al., 2011)

Although all cone cells and photoreceptor cells are present in most ommatidia in sv^spa-pol fly eyes, they display a rough eye resulting from improperly shaped and arranged cone cells.

(Shi and Noll, 2009)

sv^spa-pol homozygotes and sv^rugoso-nu/sv^spa-pol animals have rough eyes and normal body hairs.

(Sousa-Neves et al., 2005)

Mutants have cone and pigment cell defects, and photoreceptors are found in the lamina. Mutant eye discs show increased apoptosis compared to controls.

(Siddall et al., 2003)

Mutant flies have a rough eye phenotype.

(Jiao et al., 2001)

Flies show roughening across the entire eye.

(Flores et al., 2000)

Homozygotes show an almost complete absence of interommatidial bristles. A partial loss or misorientation of ommatidial structures is also seen.

(Brunner et al., 1999)

sv^spa-20/sv^spa-pol flies have a similar phenotype to sv^spa-pol flies.

(Fu et al., 1998)

Homozygotes have smaller eyes than normal. The corneal lenses and pseudocones are blurred and irregular. Numerous necrotic pits are seen between an irregular array of ommatidia of variable size. Nearly all bristles in the posterior of the eye have broken or fallen off by the third day after eclosion, although they are initially present when the adults eclose. Most bristles in the anterior of the eye are misplaced or project as doublets from the same vertex. Many ommatidia in adults have the normal number of photoreceptor cells, but show abnormal localisation and orientation of the photoreceptor cells and have malformed rhabdomeres. Other ommatidia have lost between 2 to 5 photoreceptor cells. Many ommatidia appear fused to each other. Cone cells retain their immature round shape and fail to adopt the typical rhomboid-like configuration 24 hours after puparium formation (APF), and some ommatidia lack one of the primary pigment cells. At 45 hours APF most ommatidia are disorganised and their regular lattice is disrupted. The cone cells are abnormal sizes and shapes and fail to form proper contacts with one another, and occasionally one cone cell is lost. Approximately half the ommatidia have lost one or two primary pigment cells, and the remaining primary pigment cell is enlarged and may enclose three of the four cone cells. There are frequently no cells remaining between primary pigment cells of adjacent ommatidia.

(Fu and Noll, 1997)

sv^spa-pol/sv^spa-1 has a more extreme phenotype than sv^spa-1/sv^spa-1.

(Sturtevant, 1961)

Eyes rather small; surface smooth and glassy. During second day of pupal life, retinula cells withdraw from other cells of eye disk. SEM studies show irregular disposition and morphology of ommatidial hairs as well as numerous necrotic pits over surface of eye (Oster and Crang, 1972); Strum-Tegethoff and Dicke, 1974). ERG absent (Grossfield, 1975). Homozygote has excellent viability and fertility. RK1.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

sv^spa-pol has visible phenotype, enhanceable by Poxn^hs.sev

(Jiao et al., 2001)

Suppressed by

Statement

Reference

sv^spa-pol has increased cell death phenotype, suppressible by BacA\p35^GMR.PH

(Siddall et al., 2003)

Enhancer of

Statement

Reference

sv^spa-pol/sv[+] is an enhancer of visible phenotype of EBV\BZLF1^GMR.PA

(Adamson et al., 2005)

sv^spa-pol/sv[+] is an enhancer of abnormal size | adult stage phenotype of EBV\BZLF1^GMR.PA

(Adamson et al., 2005)

sv^spa-pol/sv[+] is an enhancer of visible phenotype of rg^unspecified

(Shamloula et al., 2002)

sv^spa-pol is an enhancer of visible phenotype of Poxn^UAS.cJa, Scer\GAL4^sv.PJ

(Jiao et al., 2001)

sv^spa-pol is an enhancer of visible | dominant phenotype of wg^hs.2sev

(Brunner et al., 1999)

Other

Statement

Reference

BacA\p35^GMR.PH, sv^spa-pol has visible phenotype

(Siddall et al., 2003)

Poxn^sv.PJ, sv^spa-pol has visible phenotype

(Jiao et al., 2001)

Phenotype Manifest In

Enhanced by

Statement

Reference

sv^spa-pol has lens | somatic clone phenotype, enhanceable by pros[+]/pros¹⁷

(Charlton-Perkins et al., 2011)

sv^spa-pol has cone cell | somatic clone phenotype, enhanceable by pros¹⁷

(Charlton-Perkins et al., 2011)

sv^spa-pol has cone cell phenotype, enhanceable by Poxn^hs.sev

(Jiao et al., 2001)

sv^spa-pol has eye phenotype, enhanceable by Poxn^hs.sev

(Jiao et al., 2001)

Suppressed by

Statement

Reference

sv^spa-pol has eye disc phenotype, suppressible by BacA\p35^GMR.PH

(Siddall et al., 2003)

Enhancer of

Statement

Reference

sv^spa-pol is an enhancer of rhabdomere R7 | increased number phenotype of Scer\GAL4^sev.EP, pros^L.UAS

(Charlton-Perkins et al., 2011)

sv^spa-pol/sv[+] is an enhancer of eye phenotype of EBV\BZLF1^GMR.PA

(Adamson et al., 2005)

sv^spa-pol/sv[+] is an enhancer of eye phenotype of rg^unspecified

(Shamloula et al., 2002)

sv^spa-pol is an enhancer of eye phenotype of Poxn^UAS.cJa, Scer\GAL4^sv.PJ

(Jiao et al., 2001)

sv^spa-pol is an enhancer of ommatidium phenotype of Poxn^UAS.cJa, Scer\GAL4^sv.PJ

(Jiao et al., 2001)

sv^spa-pol/sv[+] is an enhancer of phenotype of lz^ts1

(Flores et al., 2000)

Suppressor of

Statement

Reference

sv^spa-pol is a suppressor of cone cell | increased number phenotype of Scer\GAL4^sev.EP, pros^L.UAS

(Charlton-Perkins et al., 2011)

Other

Statement

Reference

Scer\GAL4^sev.EP, pros^L.UAS, sv^spa-pol has lens phenotype

(Charlton-Perkins et al., 2011)

sv^spa-pol, ttk¹ has eye phenotype

(Shi and Noll, 2009)

sv^spa-pol, ttk¹ has ommatidium phenotype

(Shi and Noll, 2009)

sv^spa-pol, ttk¹ has interommatidial bristle phenotype

(Shi and Noll, 2009)

sv^spa-pol, ttk¹ has eye photoreceptor cell phenotype

(Shi and Noll, 2009)

sv^spa-pol, ttk¹ has cone cell | pupal stage phenotype

(Shi and Noll, 2009)

BacA\p35^GMR.PH, sv^spa-pol has cone cell phenotype

(Siddall et al., 2003)

BacA\p35^GMR.PH, sv^spa-pol has secondary pigment cell phenotype

(Siddall et al., 2003)

Poxn^hs.sev, sv^spa-pol has lens phenotype

(Jiao et al., 2001)

Poxn^sv.PJ, sv^spa-pol has eye phenotype

(Jiao et al., 2001)

Poxn^sv.PJ, sv^spa-pol has ommatidium phenotype

(Jiao et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

pros¹⁷ mutants that are also heterozygous for pros¹⁷ show a much more severe lens phenotype than either individual mutant, with many ommatidia having reduced, defective, or missing lenses. These mutants also display a reduced number of cone cells per ommatidia.

Lens structures are missing in pros¹⁷, sv^spa-pol double mutants. No cone cells are detected in these mutants.

Overexpression of pros^L.Scer\UAS under the control of Scer\GAL4^sev.EP in homozygous sv^spa-pol mutant eyes results in a significant increase in R7 photoreceptor numbers, with three to four often present in individual ommatidia. In addition, no cone cells are detected and adult lenses fail to form.

(Charlton-Perkins et al., 2011)

sv^spa-pol ttk¹ double mutant eyes show a much stronger rough eye phenotype than either single mutant: the surface of the eye is nearly flat with irregularly spaced bristles. Only a few malformed ommatidia, many of which have open holes at their apices, are visible. Photoreceptors in the remaining ommatidia have strongly deformed rhabdomeres. sv^spa-pol ttk¹ pupal eye discs have no cone cells.

(Shi and Noll, 2009)

The increased cell death seen in sv^spa-pol eye discs is blocked by BacA\p35^GMR.PH, although the adult eye is not rescued in these flies, and it lacks cone cells but has excess secondary pigment cells.

(Siddall et al., 2003)

The sv^spa-pol eye phenotype is enhanced by Poxn^hs.sev, with the eye lacking all lenses and most bristles. The number of cone cells present in mid-pupal eye discs is reduced compared to sv^spa-pol single mutants and those that are seen are smaller than normal and seem to be undergoing apoptosis. Flies carrying two copies of Poxn^sv.PJ in a sv^spa-pol/+ background have a weak rough eye phenotype, which is stronger in a homozygous sv^spa-pol background.

(Jiao et al., 2001)

sv^spa-pol dominantly enhances the lz^ts1 phenotype.

(Flores et al., 2000)

Strongly enhances the phenotype of wg^hs.2sev.

(Brunner et al., 1999)

Xenogenetic Interactions

Statement

Reference

The eye phenotype of flies carrying a weakly expressing P{GMR-BZLF1.A} line is enhanced by sv^spa-pol/+.

(Adamson et al., 2005)

Complementation and Rescue Data

Fails to complement

sv^rugoso-nu

(Sousa-Neves et al., 2005)

sv^4G

(Kaminker et al., 2001)

sv^I

(Kaminker et al., 2001)

sv^spa-20

(Fu et al., 1998)

Rescued by

sv^spa-pol is rescued by sv^+mFa

(Flores et al., 2000)

sv^spa-pol is rescued by sv^+m6

(Flores et al., 2000)

sv^spa-pol is rescued by sv^+m1

(Flores et al., 2000)

sv^spa-pol is rescued by sv^+m2

(Flores et al., 2000)

sv^spa-pol is rescued by sv^+m4

(Flores et al., 2000)

sv^spa-pol is rescued by sv^+mFa

(Fu et al., 1998)

sv^spa-pol is rescued by sv^+mFa

(Fu and Noll, 1997)

Partially rescued by

sv^spa-pol is partially rescued by sv^m11

(Flores et al., 2000)

sv^spa-pol is partially rescued by sv^m13

(Flores et al., 2000)

sv^spa-pol is partially rescued by sv^m7

(Flores et al., 2000)

sv^spa-pol is partially rescued by sv^m10

(Flores et al., 2000)

sv^spa-pol is partially rescued by sv^m9

(Flores et al., 2000)

sv^spa-pol is partially rescued by sv^m12

(Flores et al., 2000)

sv^spa-pol is partially rescued by sv^m14

(Flores et al., 2000)

sv^spa-pol is partially rescued by sv^m3

(Flores et al., 2000)

sv^spa-pol is partially rescued by sv^hs.PF

(Fu et al., 1998)

Not rescued by

sv^spa-pol is not rescued by sv^mRDx3

(Flores et al., 2000)

Comments

The sv^spa-pol eye phenotype is partially rescued by expression of sv^hs.PF using heat shock.

(Fu et al., 1998)

The sv^spa-pol phenotype is completely rescued by sv^+mFa.

(Fu and Noll, 1997)

Images (1)

Holtzman, S. and Kaufman, T. (2013)

Mutant

Wild-type

Stocks (81)

Bloomington

1566

sv^spa-pol

4236

mei-41^D5 f¹; sv^spa-pol

3698

y¹ v¹; C(4)RM, sv^spa-pol

84094

w^*/P{hsp26-pt-T}7-M143; sv^spa-pol

3524

y¹; Df(3R)Po2, cv-c¹/TM2; sv^spa-pol

3525

y¹; Df(3R)Po3, cv-c¹/TM2; sv^spa-pol

3526

y¹; Df(3R)Po4, cv-c¹/TM2; sv^spa-pol

Kyoto

101789

sv^spa-pol

101785

ci^D sv^spa-pol / l(4)102CDq^*

106412

winscy; dpy^ov1; rho^ve-1; sv^spa-pol

107082

y¹; Df(3R)Po2, cv-c¹/TM2; sv^spa-pol

107083

y¹; Df(3R)Po3, cv-c¹/TM2; sv^spa-pol

More stocks available...

Notes on Origin

Discoverer

Hadorn, Jan. 1951.

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

D-Pax2^spa-pol

(Kaminker et al., 2001)

Df(4)spa^pol

(Flores et al., 2000)

pol

spa^pol

(Charlton-Perkins et al., 2011, Dziedzic et al., 2009, Shi and Noll, 2009, Blanco et al., 2005, Siddall et al., 2003, Punzo et al., 2002, Shamloula et al., 2002, Jiao et al., 2001, Negishi et al., 2001, Flores et al., 2000, Green and Piergentili, 2000, Brunner et al., 1999, Kavaler et al., 1999, Fu et al., 1998)

sv^spa-pol

(Binder et al., 2025, Valtonen et al., 2014, Cook, 2007.12.19)

Name Synonyms

Sparkling-poliert

(Green and Piergentili, 2000)

poliert

polished

(Cook, 2007.12.19)

Secondary FlyBase IDs

References (32)

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