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General Information
Symbol
Dmel\trx1
Species
D. melanogaster
Name
FlyBase ID
FBal0017165
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Mutagen
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Linked to:
EcoRI-EcoRI restriction fragment
Comment:

position of restriction fragment on reference sequence inferred by FlyBase curator

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

trx1 is associated with an approximately 9kb insert in the region encoding the first intron of trx. This mutant appears to encode normal trx protein, that is produced in reduced amounts.

9kb insertion upstream of the coding region, possibly a novel mobile element.

9.2kb insertion of unknown DNA within intron.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

trx1 heterozygous adults show occasional homeotic transformation in the abdomen (A5 to A4 transformation, indicated by loss of pigmentation in A5).

Heterozygous adults display transformation of the fifth abdominal segment towards the likelihood of A5, and frequently show a partial haltere-to-wing transformation.

trx1 flies show a haltere to wing transformation.

trx1/trxB11 flies show transformations of wing to haltere; 49% show strong transformation, 34% show weak transformation and 16% show no transformation.

trx1/trxE2 flies show transformations of wing to haltere; 57% show strong transformation, 34% show weak transformation and 9% show no transformation.

Homozygotes show homeotic transformation phenotypes. They show an average of 7.16 transformations/fly (males were scored for a possible 29 transformations, females were scored for a possible 26 transformations). These phenotypes have an increased penetrance at 25oC. 0.8% of heterozygous adults show at least one transformation phenotype. trx1 in combination with other trx alleles produces patchy transformations that occur most frequently in anterior compartments. trx1/trxred-P6 hemizygotes show phenotypes that suggest decreased Scr function (transformation of T1 structures to a T2 identity), decreased Ubx function (transformation of T3 to T2), decreased abd-A function (transformation of A2-A7 to A1) and decreased Abd-B function (transformation of posterior abdominal segments to more anterior abdominal identities). Scr group transformations include the development of mesothoracic tibial preapical bristles or mesothoracic tibial apical bristles on the prothoracic tibia, reduced numbers of sex comb teeth (in males), development of sternopleural bristles or katepisternal bristles on the T1 segment. Ubx group transformations include haltere to wing, metathoracic laterotergite to scutum, the development of T3 sternopleural bristles, mesothoracic tibial preapical bristles or mesothoracic tibial apical bristles on the metathoracic tibia. abd-A group transformations include, the development of A1 type bristles on abdominal tergites 2. Abd-B group transformations include, the pigmentation of abdominal tergites 5 to 6 in males, and enlargement of abdominal tergite 7.

Loss of trx in enF4 flies enhances the wing phenotype; severe disruption to posterior wing development. These flies are also female sterile.

Temperature shift experiments reveal temperature sensitive period is prior to gastrulation.

Transformation of the posterior abdominal segments to anterior abdominal segments.

Flies homozygous for trx1 show a variety of partial homeotic transformations <up>ventral prothorax and metathorax to mesothorax and second to seventh abdominal segments to first abdominal segment (Ingham and Whittle, 1980)</up>. Penetrance of the transformation phenotype is stronger in hemizygotes than homozygotes and increases as the temperature is raised from 18oC to 25oC. At the higher temperature, the penetrance of the mutant offspring of trx1/trx1 females is almost 100%, while mutant offspring of trx1/+ females show only about 50% penetrance. The temperature-sensitive period occurs prior to hatching. Ingham (1980) noted the following abnormalities in extreme trx1 mutants: (1) Extra bristles between humerus and coxa and on the distal tibia of the first leg; (2) Similar changes on the third leg; (3) Loss or reduction of transverse bristle rows, and, in males, decrease in number of sex comb teeth on the first leg; (4) Replacement of halter disk derivatives by wing blade, notal and scutellar structures; (5) Rotated genitalia and abnormal tergite pigmentation in male flies. 75% of heterozygotes with a red- deletion or with the lethal allele trx3 are lethal, either as larvae or pupae (Ingham, 1981); the heterozygotes that survive show cuticular transformations of the ventral prothorax and the metathorax, an extra mesonotum developing posterior to the normal one and anteriorly-directed abdominal transformations (Ingham, 1985a). recessive; viable in homozygotes

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

trx1 has homeotic | adult stage phenotype, enhanceable by enok1/enok[+]

trx1 has homeotic | adult stage phenotype, enhanceable by enok[+]/enok2

Suppressed by
Statement
Reference

trx1 has homeotic phenotype, suppressible by Kdm2KG04325/Kdm2[+]

trx1 has homeotic phenotype, suppressible by Kdm2EY01336/Kdm2[+]

trx1 has homeotic phenotype, suppressible by Kdm2[+]/Kdm2DG12810

trxE2/trx1 has visible | homeotic phenotype, suppressible | partially by Pp1-87B[+]/Pp1-87B1

trxB11/trx1 has visible | homeotic phenotype, suppressible | partially by Pp1-87B[+]/Pp1-87B87Bg-6

trxB11/trx1 has visible | homeotic phenotype, suppressible | partially by Pp1-87B[+]/Pp1-87B1

trxE2/trx1 has visible | homeotic phenotype, suppressible | partially by Pp1-87B[+]/Pp1-87B87Bg-6

NOT suppressed by
Statement
Reference

trx1 has visible | homeotic phenotype, non-suppressible by Df(2R)ED3921

Enhancer of
Statement
Reference

trx[+]/trx1 is an enhancer of visible | homeotic phenotype of Dsp11

NOT Enhancer of
Statement
Reference
NOT Suppressor of
Statement
Reference

trx[+]/trx1 is a non-suppressor of visible | adult stage phenotype of Marcal1UAS.cBa, Scer\GAL4Tub.PU

trx1 is a non-suppressor of visible phenotype of BEAF-32UAS.cYa, Scer\GAL4GMR.PS

Phenotype Manifest In
Enhanced by
Statement
Reference

trx1 has adult abdominal segment 4 phenotype, enhanceable by enok1/enok[+]

trx1 has adult abdominal segment 5 phenotype, enhanceable by enok1/enok[+]

trx1 has adult abdominal segment 5 phenotype, enhanceable by enok[+]/enok2

trx1 has adult abdominal segment 4 phenotype, enhanceable by enok[+]/enok2

Suppressed by
Statement
Reference

trxE2/trx1 has wing phenotype, suppressible | partially by Pp1-87B[+]/Pp1-87B1

trxE2/trx1 has haltere | ectopic phenotype, suppressible | partially by Pp1-87B[+]/Pp1-87B1

trxB11/trx1 has wing phenotype, suppressible | partially by Pp1-87B[+]/Pp1-87B87Bg-6

trxB11/trx1 has haltere | ectopic phenotype, suppressible | partially by Pp1-87B[+]/Pp1-87B87Bg-6

trxB11/trx1 has wing phenotype, suppressible | partially by Pp1-87B[+]/Pp1-87B1

trxB11/trx1 has haltere | ectopic phenotype, suppressible | partially by Pp1-87B[+]/Pp1-87B1

trxE2/trx1 has wing phenotype, suppressible | partially by Pp1-87B[+]/Pp1-87B87Bg-6

trxE2/trx1 has haltere | ectopic phenotype, suppressible | partially by Pp1-87B[+]/Pp1-87B87Bg-6

NOT suppressed by
Statement
Reference

trx1 has haltere phenotype, non-suppressible by Df(2R)ED3921

trx1 has wing | ectopic phenotype, non-suppressible by Df(2R)ED3921

Enhancer of
Statement
Reference

trx[+]/trx1 is an enhancer of eye phenotype of Hsap\MECP2UAS.FL, Scer\GAL4GMR.PU

trx[+]/trx1 is an enhancer of eye phenotype of Hsap\MECP2Δ166.UAS, Scer\GAL4GMR.PU

trx[+]/trx1 is an enhancer of wing | ectopic phenotype of Dsp11

trx[+]/trx1 is an enhancer of haltere phenotype of Dsp11

NOT Enhancer of
Statement
Reference

trx[+]/trx1 is a non-enhancer of wing vein | adult stage phenotype of Marcal1UAS.cBa, Scer\GAL4Tub.PU

trx[+]/trx1 is a non-enhancer of wing vein | ectopic phenotype of Snr1E1

trx1 is a non-enhancer of eye phenotype of BEAF-32UAS.cYa, Scer\GAL4GMR.PS

Suppressor of
Statement
Reference

trx1 is a suppressor of eye phenotype of CycEJP

NOT Suppressor of
Statement
Reference

trx[+]/trx1 is a non-suppressor of wing vein | adult stage phenotype of Marcal1UAS.cBa, Scer\GAL4Tub.PU

trx[+]/trx1 is a non-suppressor of wing vein | ectopic phenotype of Snr1E1

trx1 is a non-suppressor of eye phenotype of BEAF-32UAS.cYa, Scer\GAL4GMR.PS

Additional Comments
Genetic Interactions
Statement
Reference

The formation of ectopic wing veins observed in adult flies expressing Marcal1Scer\UAS.cBa under the control of Scer\GAL4tub.PU is not affected by combination with a single copy of trx1.

The frequency of homeotic transformations is strikingly reduced in flies transheterozygous for trx1 and Kdm2KG04325, Kdm2EY01336 or Kdm2DG12810, compared to flies heterozygous for trx1 alone.

Df(2R)ED3921 fails to suppress the haltere to wing transformation of trx1 flies.

Pp1-87B87Bg-6 dominantly suppresses the transformation of wing to haltere seen in trx1/trxE2 flies; 36% show strong transformation, 42% show weak transformation and 22% show no transformation.

Pp1-87B1 dominantly suppresses the transformation of wing to haltere seen in trx1/trxE2 flies; 31% show strong transformation, 39% show weak transformation and 30% show no transformation.

Pp1-87B87Bg-6 dominantly suppresses the transformation of wing to haltere seen in trx1/trxB11 flies; 28% show strong transformation, 29% show weak transformation and 43% show no transformation.

Pp1-87B1 dominantly suppresses the transformation of wing to haltere seen in trx1/trxB11 flies; 27% show strong transformation, 24% show weak transformation and 49% show no transformation.

Expression of Pp1-87BScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4arm.PS has no effect on the transformation of wing to haltere seen in trx1/trxE2 and trx1/trxB11 flies.

Mutant suppresses the CycEJP eye rough phenotype.

The frequency of transformation of halteres into wings seen in Dsp11/Y flies is enhanced by trx1/+.

Has little or no effect on the phenotype of E2fGMR.PD DpGMR.PD BacA\p35GMR.PH flies.

Xenogenetic Interactions
Statement
Reference

The formation of ectopic wing veins observed in adult flies expressing Hsap\SMARCAL1Scer\UAS.cBa under the control of Scer\GAL4Bx-MS1096 is not affected by combination with a single copy of trx1.

Complementation and Rescue Data
Comments

Partial complementation between hypomorphs produced phenotypes that were weaker than either of the hemizygous or homozygous phenotypes, either rescuing lethality or producing a weaker homeotic transformation phenotype. Transformations range from an average of 0.01 to 4.82 transformations/fly (males were scored for a possible 29 transformations, females were scored for a possible 26 transformations) in different allelic combinations.

Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer

Ingham.

Comments
Comments

The following alleles form a series of progressively increasing penetrance and expressivity of phenotype when in transheterozygous combination with trx1 : trxM17 > trxZ32 > trxZ16 > trxZ11 > trxM18 = trxJY16 > trxE3.

Weak trx allele.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
References (36)