FlyBase Allele Report: Dmel\twe[1]

General Information

Symbol

Dmel\twe¹

Species

D. melanogaster

Name

FlyBase ID

FBal0017295

Feature type

allele

Associated gene

Dmel\twe

Associated Insertion(s)

Carried in Construct

Also Known As

twe^HB5, twine^HB5, mat(2)synHB5

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(White-Cooper et al., 1993)

Mutagen

ethyl methanesulfonate

(Schupbach and Wieschaus, 1989)

Progenitor genotype

Cytology

Description

Proline to leucine substitution at amino acid position 295 in HC motif of twe protein.

(Courtot et al., 1992)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:16,260,529..16,260,529 [-]

Nucleotide change:

C16260529T

Amino acid change:

P326L | twe-PA; P326L | twe-PB; P326L | twe-PC; P326L | twe-PD

(Courtot et al., 1992)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

oocyte

(Von Stetina et al., 2008)

oocyte nucleus

(Xiang et al., 2007)

primordial germ cell | maternal effect

(Schupbach and Wieschaus, 1989)

spermatocyte

(Lin et al., 1996)

spindle

(Sigrist et al., 1995, White-Cooper et al., 1993)

spindle & oocyte

(Von Stetina et al., 2008)

spindle | embryonic stage

(Von Stetina et al., 2008)

yolk

(Von Stetina et al., 2008)

Detailed Description

Statement

Reference

4.3% of late stage 13 oocytes show metaphase I arrest in twe¹ females, while 84% of the mutant oocytes are still in prophase I at this stage (virtually all wild-type oocytes are in metaphase I at this stage). At stage 14, 83% of mutant oocytes have abnormal DNA morphology. 82% of the mutant oocytes fail to form or maintain the meiotic spindle at stage 14 and 18% have abnormal spindle masses with DNA attached.

The nuclear envelope persists for longer than normal in twe¹ oocytes.

Stage 14 mutant oocytes are not dehydrated (in contrast to wild-type oocytes at this stage) and have abnormal yolk morphology.

96% of 0- to 3-hour embryos derived from homozygous females have dispersed or undetectable DNA. Of these, approximately half have abnormal spindles associated with DNA masses, whereas 8% have free spindle asters and/or thin, long spindles.

(Von Stetina et al., 2008)

Homozygous females show delayed nuclear envelope breakdown in the oocyte; the nucleus is still present until early stage 14.

(Xiang et al., 2007)

Male sterile but female fertile when transheterozygous with twe^k08310 or twe^EP613.

(Ashburner et al., 1999)

When heterozygous with twe^ZO758 meiotic entry occurs and the phenotype is similar to loss of function twe or bol alleles.

(Maines and Wasserman, 1999)

Primary spermatocytes entering the first meiotic division have bivalents with only partially condensed chromosomes. Chromosome segregation and cytokinesis fail to occur and meiotic spindles do not form in the spermatocytes. Meiotic arrest does not occur. Nucleolar breakdown occurs and the cells proceeds to the round spermatid stage, and mitochondrial aggregates form. Eventually the bivalents decondense and assume a thin crescent shape apposed to the nuclear membrane.

(Lin et al., 1996)

Meiotic spindles and metaphase plates are never formed at high temperatures. Chromosomes still condense in late spermatocytes and spermatid differentiation continues. Heat induced expression of stg^hs.PE3 restores spindle formation in testis.

(Sigrist et al., 1995)

Mutation prevents spindle formation during the entry into meiosis in males but chromosome condensation and nuclear envelope breakdown still occur. Testes contain disordered sperm heads on partially elongated sperm. Meiotic spindles do form in females but appear abnormal. Female meiotic divisions do not arrest at metaphase I as in wild type but continue repeatedly, leading to gross non-disjunction. Small chromatin masses often segregate properly to the spindle poles and persist into the embryos where they appear to participate in mitotic divisions on thin spindles. In addition the embryos contain a small number of large chromatin masses that are not associated with spindles.

(White-Cooper et al., 1993)

Large proportion of eggs derived from homozygous twe¹ mothers lack nuclear structures or have 5 enlarged 'nuclei' within them. The few reamining undergo a number of normal mitotic cycles. Meiosis does not occur in homozygous twe¹ males.

(Alphey et al., 1992)

Block of meiosis in males and severe meiotic defects in females.

(Courtot et al., 1992)

Eggs derived from homozygous females initiate development and cytoplasmic clearing occurs in a narrow zone around the egg periphery (in wild-type embryos this process is coupled to the arrival of the nuclei at the periphery). This cytoplasmic clearing appears irregular. The eggs do not seem to develop beyond this stage; pole cells are not formed and cellularisation does not occur. However, about two hours after cytoplasmic clearing, the egg periphery starts to show local contractions, in what might be an attempt at gastrulation.

(Schupbach and Wieschaus, 1989)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

twe¹/twe[+] is a suppressor of male sterile phenotype of rux⁸

(Thomas et al., 1994)

Other

Statement

Reference

stg², twe¹ has decreased cell number | oogenesis phenotype

(Edgar and Datar, 1996)

stg², twe¹ has decreased occurrence of cell division | oogenesis phenotype

(Edgar and Datar, 1996)

Phenotype Manifest In

Suppressor of

Statement

Reference

twe¹ is a suppressor of phenotype of rux⁹

(Gonczy et al., 1994)

twe¹ is a suppressor of phenotype of rux^unspecified

(Gonczy et al., 1994)

NOT Suppressor of

Statement

Reference

twe¹/twe[+] is a non-suppressor of eye phenotype of rux⁸

(Thomas et al., 1994)

Other

Statement

Reference

stg², twe¹ has ovariole phenotype

(Edgar and Datar, 1996)

stg², twe¹ has female germline cell | oogenesis phenotype

(Edgar and Datar, 1996)

Additional Comments

Genetic Interactions

Statement

Reference

Simultaneous deletion of stg²/stg² twe¹/twe¹ causes a block in germ cell proliferation, no ovarioles or eggs are produced.

(Edgar and Datar, 1996)

Lowering the dose of twe rescues the rux germline phenotype.

(Gonczy et al., 1994)

Removing one copy of twe rescues the sterility, but not the rough eye phenotype, of rux⁸.

(Thomas et al., 1994)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

twe¹ is rescued by Scer\GAL4^{VP16.nanos.UTR}/twe^UASp.Tag:MYC

(Von Stetina et al., 2008)

twe¹ is rescued by twe^hs.PVS

(Von Stetina et al., 2008)

twe¹ is rescued by twe^βTub85D.PM

(Maines and Wasserman, 1999)

twe¹ is rescued by twe^+t3.7

(Alphey et al., 1992)

twe¹ is rescued by twe^+t10

(Alphey et al., 1992)

Comments

Mutant phenotype can be rescued by the introduction of the wild type gene by P element mediated transformation.

(Alphey et al., 1992)

Images (0)

Mutant

Wild-type

Stocks (2)

4274

107663

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

HB5

(Gonczy et al., 1994)

mat(2)syn^HB5

(Courtot et al., 1992)

mat(2)synHB5

(Thomas et al., 1994, Schupbach and Wieschaus, 1989)

twe¹

(Das et al., 2016, Anderson et al., 2009, Rautureau et al., 2009, Von Stetina et al., 2008, Xiang et al., 2007)

twe^HB5

(Deneke et al., 2016, Baker and Fuller, 2007, Tadros et al., 2003, Ashburner et al., 1999, Maines and Wasserman, 1999, Eberhart and Wasserman, 1995, Alphey et al., 1992)

twine^HB5

(Liu et al., 2020, Hara et al., 2017, White-Cooper et al., 1998, Sigrist et al., 1995, White-Cooper et al., 1993, White-Cooper et al., 1993)

twineHB5

(Petrova et al., 2018)

twn^HB5

(Edgar and Datar, 1996)

Name Synonyms

twine1

(Von Stetina et al., 2008)

Secondary FlyBase IDs

References (28)

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