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General Information
Symbol
Dmel\twe1
Species
D. melanogaster
Name
FlyBase ID
FBal0017295
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
tweHB5, twineHB5, mat(2)synHB5
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

C16260529T

Amino acid change:

P326L | twe-PA; P326L | twe-PB; P326L | twe-PC; P326L | twe-PD

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Proline to leucine substitution at amino acid position 295 in HC motif of twe protein.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

4.3% of late stage 13 oocytes show metaphase I arrest in twe1 females, while 84% of the mutant oocytes are still in prophase I at this stage (virtually all wild-type oocytes are in metaphase I at this stage). At stage 14, 83% of mutant oocytes have abnormal DNA morphology. 82% of the mutant oocytes fail to form or maintain the meiotic spindle at stage 14 and 18% have abnormal spindle masses with DNA attached.

The nuclear envelope persists for longer than normal in twe1 oocytes.

Stage 14 mutant oocytes are not dehydrated (in contrast to wild-type oocytes at this stage) and have abnormal yolk morphology.

96% of 0- to 3-hour embryos derived from homozygous females have dispersed or undetectable DNA. Of these, approximately half have abnormal spindles associated with DNA masses, whereas 8% have free spindle asters and/or thin, long spindles.

Homozygous females show delayed nuclear envelope breakdown in the oocyte; the nucleus is still present until early stage 14.

Male sterile but female fertile when transheterozygous with twek08310 or tweEP613.

When heterozygous with tweZO758 meiotic entry occurs and the phenotype is similar to loss of function twe or bol alleles.

Primary spermatocytes entering the first meiotic division have bivalents with only partially condensed chromosomes. Chromosome segregation and cytokinesis fail to occur and meiotic spindles do not form in the spermatocytes. Meiotic arrest does not occur. Nucleolar breakdown occurs and the cells proceeds to the round spermatid stage, and mitochondrial aggregates form. Eventually the bivalents decondense and assume a thin crescent shape apposed to the nuclear membrane.

Meiotic spindles and metaphase plates are never formed at high temperatures. Chromosomes still condense in late spermatocytes and spermatid differentiation continues. Heat induced expression of stghs.PE3 restores spindle formation in testis.

Mutation prevents spindle formation during the entry into meiosis in males but chromosome condensation and nuclear envelope breakdown still occur. Testes contain disordered sperm heads on partially elongated sperm. Meiotic spindles do form in females but appear abnormal. Female meiotic divisions do not arrest at metaphase I as in wild type but continue repeatedly, leading to gross non-disjunction. Small chromatin masses often segregate properly to the spindle poles and persist into the embryos where they appear to participate in mitotic divisions on thin spindles. In addition the embryos contain a small number of large chromatin masses that are not associated with spindles.

Large proportion of eggs derived from homozygous twe1 mothers lack nuclear structures or have 5 enlarged 'nuclei' within them. The few reamining undergo a number of normal mitotic cycles. Meiosis does not occur in homozygous twe1 males.

Block of meiosis in males and severe meiotic defects in females.

Eggs derived from homozygous females initiate development and cytoplasmic clearing occurs in a narrow zone around the egg periphery (in wild-type embryos this process is coupled to the arrival of the nuclei at the periphery). This cytoplasmic clearing appears irregular. The eggs do not seem to develop beyond this stage; pole cells are not formed and cellularisation does not occur. However, about two hours after cytoplasmic clearing, the egg periphery starts to show local contractions, in what might be an attempt at gastrulation.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement
Reference

twe1/twe[+] is a suppressor of male sterile phenotype of rux8

Other
Phenotype Manifest In
Suppressor of
Statement
Reference

twe1 is a suppressor of phenotype of rux9

twe1 is a suppressor of phenotype of ruxunspecified

NOT Suppressor of
Statement
Reference

twe1/twe[+] is a non-suppressor of eye phenotype of rux8

Other
Additional Comments
Genetic Interactions
Statement
Reference

Simultaneous deletion of stg2/stg2 twe1/twe1 causes a block in germ cell proliferation, no ovarioles or eggs are produced.

Lowering the dose of twe rescues the rux germline phenotype.

Removing one copy of twe rescues the sterility, but not the rough eye phenotype, of rux8.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Mutant phenotype can be rescued by the introduction of the wild type gene by P element mediated transformation.

Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (9)
References (28)