Sequencing revealed that there is no amino acid replacement in the coding sequence.
Strong abdominal defects.
Absence of posterior pole plasm, polar granules and pole cells.
Females retain some residual vas gene activity as eggs are laid at a low frequency, some develop into embryos with abdominal deletions and no pole cells.
vas1/vas3 has embryonic abdominal segment phenotype, suppressible | partially by nosnos.+2'ME-3X.Tag:HA
vas3 has embryonic abdominal segment phenotype, suppressible by nosαTub84B.UTR.Tag:HA
vas1/vas3 has embryonic abdominal segment phenotype, non-suppressible by nosnos.+1.+2'
vas1/vas3 has embryonic abdominal segment phenotype, non-suppressible by nosnos.+2RO.Tag:HA
vas3 has phenotype, non-suppressible by nosαTub84B.UTR.virTCE.Tag:HA
vas3 has phenotype, non-suppressible by nosnos.+1.Hsp83
nosnos.+2'ME-3X.T:Ivir\HA1 partially rescues the abdominal segmentation defect of embryos derived from vas1/vas3 females; 2% of the embryos develop no abdominal segments, 53% develop 1-3 abdominal segments, 43% develop 4-6 abdominal segments and 2% develop 7-8 abdominal segments. The embryos do not have anterior defects. nosnos.+1.+2' or nosnos.+2RO.T:Ivir\HA1 fail to rescue the abdominal segmentation defect of embryos derived from vas1/vas3 females; 100% of the embryos develop no abdominal segments. The embryos do not have anterior defects.
The lack of abdominal segments seen in embryos derived from vas1/vas3 females is rescued if the females also carry nosαTub84B.UTR.T:Ivir\HA1, but not if they carry nosαTub84B.UTR.virTCE.T:Ivir\HA1 or nosnos.+1.Hsp83.
Embryos derived from vas1/vas3 females carrying nosnos.+2.Hsp83 or nosnos.+2-3X.T:Ivir\HA1 do not produce any abdominal segments. 98% of embryos derived from vas1/vas3 females carrying nosnos.+2'.Hsp83 produce 7-8 abdominal segments and 58% of these embryos show loss of head or thorax structures. Embryos derived from vas1/vas3 females carrying nosnos.+2'-2X.T:Ivir\HA1 develop 0-3 abdominal segments in 16% of cases, 4-6 abdominal segments in 51% of cases and 7-8 abdominal segments in 34% of cases. Embryos derived from vas1/vas3 females carrying nosnos.+2'-3X.T:Ivir\HA1 develop 0-3 abdominal segments in 72% of cases and 4-6 abdominal segments in 28% of cases.
vas1/vas3 is rescued by Scer\GAL4VP16.nos.UTR/vasUAS.GFP
vas1/vas3 is rescued by vasαTub67C.GFP
vas1/vas3 is not rescued by Scer\GAL4VP16.nos.UTR/vasDQAD.UAS.GFP
Expression of vasDQAD.Scer\UAS.T:Avic\GFP using Scer\GAL4nos.UTR.T:Hsim\VP16 fails to rescue the infertility phenotype of vas1/vas3 mutants.
When vasScer\UAS.T:Avic\GFP is expressed using Scer\GAL4nos.UTR.T:Hsim\VP16 in a vas1/vas3 mutant background, it rescues infertility. The rescued embryos show normal segmentation patterns, very similar to those hatched from eggs laid by wild-type females.
vasαTub67C.T:Avic\GFP rescues the abdominal and germ-line defects of vas1/vas3 mutant embryos.
Expression of Ecol\lacZP\T.1911-2183.hs in vas3 flies shows that vas protein is not required for germ-line specific splicing of the P-element third intron.
Strong allele of vas. Cytoplasmic transplantation of wild type plasm into the abdominal region restores normal abdominal development.