Open Close
General Information
Symbol
Dmel\vas3
Species
D. melanogaster
Name
FlyBase ID
FBal0017847
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
vasD1
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Sequencing revealed that there is no amino acid replacement in the coding sequence.

Expression Data
Reporter Expression
Additional Information
Statement
Reference

vas protein is undetectable in ovaries from vas3 and vas7 mothers.

 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

vas15/vas3 females produce eggs with more severe anterior-posterior and dorsal-ventral polarity phenotypes than homozygous vas15 females. vas7/vas3 females produce eggs with similar phenotypes to homozygous vas7 females.

Strong abdominal defects.

Absence of posterior pole plasm, polar granules and pole cells.

Females retain some residual vas gene activity as eggs are laid at a low frequency, some develop into embryos with abdominal deletions and no pole cells.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
NOT suppressed by
Statement
Reference

vas3 has phenotype, non-suppressible by nosαTub84B.UTR.virTCE.Tag:HA

vas3 has phenotype, non-suppressible by nosnos.+1.Hsp83

Additional Comments
Genetic Interactions
Statement
Reference

nosnos.+2'ME-3X.T:Ivir\HA1 partially rescues the abdominal segmentation defect of embryos derived from vas1/vas3 females; 2% of the embryos develop no abdominal segments, 53% develop 1-3 abdominal segments, 43% develop 4-6 abdominal segments and 2% develop 7-8 abdominal segments. The embryos do not have anterior defects. nosnos.+1.+2' or nosnos.+2RO.T:Ivir\HA1 fail to rescue the abdominal segmentation defect of embryos derived from vas1/vas3 females; 100% of the embryos develop no abdominal segments. The embryos do not have anterior defects.

The lack of abdominal segments seen in embryos derived from vas1/vas3 females is rescued if the females also carry nosαTub84B.UTR.T:Ivir\HA1, but not if they carry nosαTub84B.UTR.virTCE.T:Ivir\HA1 or nosnos.+1.Hsp83.

Embryos derived from vas1/vas3 females carrying nosnos.+2.Hsp83 or nosnos.+2-3X.T:Ivir\HA1 do not produce any abdominal segments. 98% of embryos derived from vas1/vas3 females carrying nosnos.+2'.Hsp83 produce 7-8 abdominal segments and 58% of these embryos show loss of head or thorax structures. Embryos derived from vas1/vas3 females carrying nosnos.+2'-2X.T:Ivir\HA1 develop 0-3 abdominal segments in 16% of cases, 4-6 abdominal segments in 51% of cases and 7-8 abdominal segments in 34% of cases. Embryos derived from vas1/vas3 females carrying nosnos.+2'-3X.T:Ivir\HA1 develop 0-3 abdominal segments in 72% of cases and 4-6 abdominal segments in 28% of cases.

Injection of nosN5 RNA into vas1/vas3 embryos completely rescues the abdominal phenotype.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of vasDQAD.Scer\UAS.T:Avic\GFP using Scer\GAL4nos.UTR.T:Hsim\VP16 fails to rescue the infertility phenotype of vas1/vas3 mutants.

When vasScer\UAS.T:Avic\GFP is expressed using Scer\GAL4nos.UTR.T:Hsim\VP16 in a vas1/vas3 mutant background, it rescues infertility. The rescued embryos show normal segmentation patterns, very similar to those hatched from eggs laid by wild-type females.

vasαTub67C.T:Avic\GFP rescues the abdominal and germ-line defects of vas1/vas3 mutant embryos.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

Expression of Ecol\lacZP\T.1911-2183.hs in vas3 flies shows that vas protein is not required for germ-line specific splicing of the P-element third intron.

Strong allele of vas. Cytoplasmic transplantation of wild type plasm into the abdominal region restores normal abdominal development.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
References (30)