FlyBase Allele Report: Dmel\vas[3]

General Information

Symbol

Dmel\vas³

Species

D. melanogaster

Name

FlyBase ID

FBal0017847

Feature type

allele

Associated gene

Dmel\vas

Associated Insertion(s)

Carried in Construct

Also Known As

vas^D1

Key Links

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Lasko and Ashburner, 1990)

Mutagen

ethyl methanesulfonate

(Ashburner et al., 1999.10.12, Ashburner, 1991, Lasko and Ashburner, 1990, Tearle and Nusslein-Volhard, 1987)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Cytology

Nature of the lesion

Statement

Reference

Sequencing revealed that there is no amino acid replacement in the coding sequence.

(Liang et al., 1994)

Expression Data

Reporter Expression

Additional Information

Statement

Reference

vas protein is undetectable in ovaries from vas³ and vas⁷ mothers.

(Lasko and Ashburner, 1990)

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile, with Scer\GAL4^VP16.nos.UTR, vas^DQAD.UAS.GFP

(Xiol et al., 2014)

female sterile (with vas¹)

(Xiol et al., 2014)

female sterile | recessive

(Ashburner et al., 1999)

lethal | maternal effect | recessive

(Lehmann and Nusslein-Volhard, 1991)

Phenotype Manifest In

egg

(Tomancak et al., 1998)

embryonic abdominal segment

(Crucs et al., 2000)

embryonic abdominal segment | maternal effect

(Lasko and Ashburner, 1990)

primordial germ cell | maternal effect

(Lasko and Ashburner, 1990)

Detailed Description

Statement

Reference

vas^EP812/vas³ and vas^k07233/vas³ females are sterile.

(Ashburner et al., 1999)

vas¹⁵/vas³ females produce eggs with more severe anterior-posterior and dorsal-ventral polarity phenotypes than homozygous vas¹⁵ females. vas⁷/vas³ females produce eggs with similar phenotypes to homozygous vas⁷ females.

(Tomancak et al., 1998)

Strong abdominal defects.

(Ephrussi et al., 1991)

Absence of posterior pole plasm, polar granules and pole cells.

(Lehmann and Nusslein-Volhard, 1991)

Females retain some residual vas gene activity as eggs are laid at a low frequency, some develop into embryos with abdominal deletions and no pole cells.

(Lasko and Ashburner, 1990)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

vas¹/vas³ has embryonic abdominal segment phenotype, suppressible | partially by nos^{nos.+2'ME-3X.Tag:HA}

(Bergsten et al., 2001)

vas³ has embryonic abdominal segment phenotype, suppressible by nos^{αTub84B.UTR.Tag:HA}

(Crucs et al., 2000)

vas¹/vas³ has abdomen phenotype, suppressible by nos^N5

(Wang and Lehmann, 1991)

NOT suppressed by

Statement

Reference

vas¹/vas³ has embryonic abdominal segment phenotype, non-suppressible by nos^nos.+1.+2'

(Bergsten et al., 2001)

vas¹/vas³ has embryonic abdominal segment phenotype, non-suppressible by nos^{nos.+2RO.Tag:HA}

(Bergsten et al., 2001)

vas³ has phenotype, non-suppressible by nos^{αTub84B.UTR.virTCE.Tag:HA}

(Crucs et al., 2000)

vas³ has phenotype, non-suppressible by nos^nos.+1.Hsp83

(Crucs et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

nos^{nos.+2'ME-3X.T:Ivir\HA1} partially rescues the abdominal segmentation defect of embryos derived from vas¹/vas³ females; 2% of the embryos develop no abdominal segments, 53% develop 1-3 abdominal segments, 43% develop 4-6 abdominal segments and 2% develop 7-8 abdominal segments. The embryos do not have anterior defects. nos^nos.+1.+2' or nos^{nos.+2RO.T:Ivir\HA1} fail to rescue the abdominal segmentation defect of embryos derived from vas¹/vas³ females; 100% of the embryos develop no abdominal segments. The embryos do not have anterior defects.

(Bergsten et al., 2001)

The lack of abdominal segments seen in embryos derived from vas¹/vas³ females is rescued if the females also carry nos^{αTub84B.UTR.T:Ivir\HA1}, but not if they carry nos^{αTub84B.UTR.virTCE.T:Ivir\HA1} or nos^nos.+1.Hsp83.

(Crucs et al., 2000)

Embryos derived from vas¹/vas³ females carrying nos^nos.+2.Hsp83 or nos^{nos.+2-3X.T:Ivir\HA1} do not produce any abdominal segments. 98% of embryos derived from vas¹/vas³ females carrying nos^{nos.+2'.Hsp83} produce 7-8 abdominal segments and 58% of these embryos show loss of head or thorax structures. Embryos derived from vas¹/vas³ females carrying nos^{nos.+2'-2X.T:Ivir\HA1} develop 0-3 abdominal segments in 16% of cases, 4-6 abdominal segments in 51% of cases and 7-8 abdominal segments in 34% of cases. Embryos derived from vas¹/vas³ females carrying nos^{nos.+2'-3X.T:Ivir\HA1} develop 0-3 abdominal segments in 72% of cases and 4-6 abdominal segments in 28% of cases.

(Bergsten and Gavis, 1999)

Injection of nos^N5 RNA into vas¹/vas³ embryos completely rescues the abdominal phenotype.

(Wang and Lehmann, 1991)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

vas¹

(Lehmann and Nusslein-Volhard, 1991)

Rescued by

vas¹/vas³ is rescued by Scer\GAL4^VP16.nos.UTR/vas^UAS.GFP

(Xiol et al., 2014)

vas¹/vas³ is rescued by vas^αTub67C.GFP

(Breitwieser et al., 1996)

Not rescued by

vas¹/vas³ is not rescued by Scer\GAL4^VP16.nos.UTR/vas^DQAD.UAS.GFP

(Xiol et al., 2014)

Comments

Expression of vas^{DQAD.Scer\UAS.T:Avic\GFP} using Scer\GAL4^{nos.UTR.T:Hsim\VP16} fails to rescue the infertility phenotype of vas¹/vas³ mutants.

When vas^{Scer\UAS.T:Avic\GFP} is expressed using Scer\GAL4^{nos.UTR.T:Hsim\VP16} in a vas¹/vas³ mutant background, it rescues infertility. The rescued embryos show normal segmentation patterns, very similar to those hatched from eggs laid by wild-type females.

(Xiol et al., 2014)

vas^{αTub67C.T:Avic\GFP} rescues the abdominal and germ-line defects of vas¹/vas³ mutant embryos.

(Breitwieser et al., 1996)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Expression of Ecol\lacZ^{P\T.1911-2183.hs} in vas³ flies shows that vas protein is not required for germ-line specific splicing of the P-element third intron.

(Ashburner, 1991)

Strong allele of vas. Cytoplasmic transplantation of wild type plasm into the abdominal region restores normal abdominal development.

(Lehmann and Nusslein-Volhard, 1991)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Name Synonyms

Secondary FlyBase IDs

References (30)

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