copia insertion into intron 2.
Insertion of a copia element in the second intron, in parallel orientation.
5.2kb parallel copia insertion into the second intron.
Parallel copia-element insertion in second intron.
Insertion of copia element.
copia insertion into an intron.
copia insertion in second chromosome.
The wa allele of the white locus has been cloned by retrieving previously cloned segments carrying a copy of the transposable element copia together with contiguous sequences (Bingham, Levis and Rubin, 1981; Gehring and Paro, 1980). Bingham and Judd (1981) have shown that copia homology is tightly linked to the site of wa, copia being located at coordinate 0.0 on the physical map of the white locus (Levis, Bingham and Rubin, 1982; Levis, O'Hare and Rubin, 1984) and inserted in the small second intron (Pirrotta and Brockl, 1984). The size of the copia insertion is 5 kb. A small amount of the normal 2.6 kb white transcript is also found in wa mutants. su(wa), which darkens the eyes of wa flies, results in a higher level of the 2.6 kb transcript, while su(f), which decreases the amount of pigment, results in a lower level of this transcript. Partial revertants of wa have been sequenced and found to retain one LTR of copia (Carbonare and Gehring, 1985; Mount et al., 1988; Zachar, Davison, Garza and Bingham, 1985b). 5.0 kb copia insertion map site (kb): 0 (by definition)
Insertion of a 5.0 kb copia-element at map position 0.0 relative to the transcription start site of w.
Flies with a wa/we(g) mutant genotype enhance the eye colour defective phenotype of g2, g50e and g53d homozygous mutants leading to a greater reduction of red pigment in the eye. wa, g53d male mutants have lower amounts of pigment in the eye than wa single mutants, leading to a change in eye colour from dull orange-red to completely white.
w1 su(f)4/wa su(f)1 females have a w1 phenotype at 24-25oC. wa su(f)4/Y males have an eye colour intermediate between that of w1 and wa at 17-18oC. wa su(f)1 flies have apricot coloured eyes at 29-30oC, at 24-25oC the eyes are near white and at 17-18oC the eyes are indistinguishable from white.