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General Information
Symbol
Dmel\ph-plac
Species
D. melanogaster
Name
FlyBase ID
FBal0024620
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
phlac
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

P{lacW} insertion is close to the first intron splice site.

Insertion components
P{lacW}ph-plac
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

ph-plac homozygous females have egg chambers with abnormal numbers of germ cells. Egg chambers in these mutants with more than 16 germ cells (23% ovarioles contain at least one of these) sometimes contain groups of nurse cells with differing degrees of polyploidy, but all contain multiple oocytes. When an egg chamber contains fewer than 15 nurse cells (5.6%), one of the adjacent chambers contains the complementary number of nurse cells or degenerating nurse cells. Whether follicles contain more or fewer than the normal number of germline cells, there are always two groups of polar cells, one at each extremity, anterior and posterior, but these groups often contain extra polar cells. Apposed eggs chambers not separated by an interfollicular stalk are also present in these mutants. Where stalks are present they are abnormal - containing 10-50 cells in 2-3 lines rather than five to seven cells organized in a single line as in wild-type. This excess of polar and stalk cells is not due to any proliferation of these cells after stage 1, and so must be due to processes occurring in the germarium. Germaria in ph-plac homozygous females display a variety of phenotypes: delayed budding of the emerging egg chambers; disorganized arrangement of encapsulated cysts; splitting of germline cysts and envelopment of fewer than 16 germline cells; an absence of prefollicular cell migration between adjacent germline cysts associated with an accumulation of several mature cysts in region 3 of the germarium (in these cases, prefollicular cell invagination can be seen anteriorly, probably prefiguring to budding of a multicyst egg chamber). In the most extreme cases there is no clear boundary between germarium and vitellarium. The number of mitotic somatic cells in regions 2b and 3 of the germarium is significantly lower for ph-plac homozygous females compared to ph-plac/+ control sisters.

Transheterozygotes with a null ph-p allele or a deficiency of ph-p cause lethality.

Mild homeotic transformation.

Homozygous viable.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhancer of
Statement
Reference
NOT Suppressor of
Statement
Reference

ph-plac/ph-p[+] is a non-suppressor of visible | adult stage phenotype of Marcal1UAS.cBa, Scer\GAL4Tub.PU

Other
Phenotype Manifest In
NOT Enhancer of
Statement
Reference
NOT Suppressor of
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference

The formation of ectopic wing veins observed in adult flies expressing Marcal1Scer\UAS.cBa under the control of Scer\GAL4tub.PU is not affected by combination with a single copy of ph-plac.

0% of ph-plac/+; en54/+ double heterozygotes and 100% of ph-plac/Y; en54/+ double heterozygotes have a gap in the fourth wing vein at 25oC. 100% of ph-plac/Y ; en1/en1 and ph-plac/Y ; en1/en54 flies have a gap in the fourth wing vein at 25oC. 20% of ph-plac/Y ; enXho25/+ flies have a gap in the fourth wing vein at 25oC.

Xenogenetic Interactions
Statement
Reference

The formation of ectopic wing veins observed in adult flies expressing Hsap\SMARCAL1Scer\UAS.cBa under the control of Scer\GAL4Bx-MS1096 is not affected by combination with a single copy of ph-plac.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (6)