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General Information
Symbol
Dmel\N264-39
Species
D. melanogaster
Name
FlyBase ID
FBal0029934
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Notch264-39, N264.39, Df(1)N264-39
Key Links
Mutagen
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Deficient for band 3C7.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Heterozygous N264-39 flies show mild wing margin notching in the distal region in about half of the wings.

Females heterozygous for N264-39, compared with wild-type, show a reduction in germarium cap cells 7 days after eclosion .

Heterozygous N264-39 wings have a mild notched wing margin, bristle loss, and slight vein thickening near the wing blades.

Stage 13 mutant male embryos have a normal number of somatic gonadal precursor cells. However, the gonads of mutant first instar male larvae lack hub cells.

The number of corpora cardiaca (CC) cells is increased in mutant stage 17 embryos compared to wild type. The CC cell lineage does not develop precociously in the mutant embryos, but multiple gl-positive CC precursors are detectable in the mutant embryos at early stage 11 (in contrast to the pair of single precursor cells seen in wild-type embryos at this stage).

Heterozygotes have a notched wing phenotype.

Zygotic N264-39 mutant embryos display a massive expansion of neuronal cells in the entire ectodermal tissue.

Flies heterozygous for N264-39 have smaller eyes than wild-type flies, but their head capsule appears of normal size.

The number of cap cells and germline stem cells in the ovaries of 2 day old heterozygous females is significantly reduced compared to wild type.

Approximately 36.36% of heterozygous N264-39 flies exhibit a haploinsufficient phenotype of wing notching at the posterior margins.

Heterozygotes show notching of the wing margin at the anterior-posterior border.

Heterozygotes have a nicked wing margin phenotype.

Boundary cells of the hindgut fail to develop in mutant embryos.

The wing-notching phenotype of N264-39 heterozygotes is more severe in flies which also carry one or two copies of EgfrE3 or EgfrE1.

Cause a complex pleiotropic syndrome including perturbations of the chaetae pattern.

Mutation causes overproduction of glioblasts.

Homozygous clones in the eye have a severely disturbed ommatidial pattern, visible as a scar in the eye surface. Ommatidia are larger than wild-type and interommatidial bristles are missing. Each ommatidium contains more retinula cells and fewer pigment cells than wild-type. Each ommatidium contains more receptor cells than normal, and may contain up to 13 receptor cells. Homozygous clones in the cuticle lack all bristles. Homozygous mutant clones in the eye are smaller and less frequent than the corresponding control clones.

Extreme embryonic neurogenic phenotype.

Lethal in combination with Nl1N-ts1 or NAx-tsl at 29oC. Homozygous lethal at 29oC.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

N264-39 has visible | dominant phenotype, enhanceable by Taf9190/e(y)1[+]

N264-39 has visible | dominant phenotype, enhanceable by hkl[+]/Acn1

N264-39 has visible | dominant phenotype, enhanceable by Acn27/Acn[+]

N264-39 has visible | dominant phenotype, enhanceable by Df(3L)vin3/+

NOT Enhanced by
Statement
Reference
Suppressed by
Statement
Reference

N264-39 has abnormal size | dominant phenotype, suppressible by crb11A22/crb[+]

N264-39 has visible | dominant phenotype, suppressible by Scer\GAL4[-]/Nedd4T119FS

NOT suppressed by
Statement
Reference

N264-39 has visible | dominant phenotype, non-suppressible by Df(3R)Pi3K92EA/+

Enhancer of
Statement
Reference
NOT Enhancer of
Statement
Reference

N[+]/N264-39 is a non-enhancer of visible phenotype of CycEJP

Suppressor of
Statement
Reference

N[+]/N264-39 is a suppressor of visible | somatic clone | heat sensitive phenotype of Uba1D6

NOT Suppressor of
Statement
Reference

N264-39 is a non-suppressor of lethal phenotype of Nf-YAUAS.Tag:HA, Scer\GAL4ey.PH

N[+]/N264-39 is a non-suppressor of visible phenotype of CycEJP

Phenotype Manifest In
Enhanced by
Statement
Reference

N264-39 has wing margin phenotype, enhanceable by Taf9190/e(y)1[+]

N264-39 has germarium cap cell phenotype, enhanceable by enok1/enok[+]

N264-39 has wing phenotype, enhanceable by hkl[+]/Acn1

N264-39 has wing phenotype, enhanceable by Acn27/Acn[+]

N264-39 has posterior wing margin phenotype, enhanceable by auxI670K/aux[+]

N264-39 has wing margin phenotype, enhanceable by Df(3L)vin3/+

NOT Enhanced by
Statement
Reference
Suppressed by
Statement
Reference
NOT suppressed by
Enhancer of
Statement
Reference

N+/N264-39 is an enhancer of wing margin phenotype of N1

N[+]/N264-39 is an enhancer of photoreceptor cell R4 phenotype of fzhs.sev

N[+]/N264-39 is an enhancer of ommatidium phenotype of fzhs.sev

N[+]/N264-39 is an enhancer of ommatidium phenotype of dshhs.sev.B

NOT Enhancer of
Statement
Reference

N[+]/N264-39 is a non-enhancer of eye phenotype of CycEJP

Suppressor of
Statement
Reference

N[+]/N264-39 is a suppressor of eye | somatic clone phenotype of Utx1

N264-39 is a suppressor of adult head | somatic clone phenotype of lsn5F3-8

N[+]/N264-39 is a suppressor of eye | somatic clone phenotype of Vps25N55

N[+]/N264-39 is a suppressor of adult head | somatic clone phenotype of Vps25N55

N264-39 is a suppressor of eye | somatic clone phenotype of lsn5F3-8

N[+]/N264-39 is a suppressor of eye | somatic clone | heat sensitive phenotype of Uba1D6

NOT Suppressor of
Statement
Reference

N[+]/N264-39 is a non-suppressor of eye | somatic clone phenotype of Vps36L5212

N[+]/N264-39 is a non-suppressor of eye phenotype of CycEJP

Additional Comments
Genetic Interactions
Statement
Reference

Combination of Scer\GAL4bbg-C96>e(y)1dsRNA.Scer\UAS.cUa and N264-39/+ results in a synergistic enhancement of the notched wing phenotype in terms of both penetrance and expressivity.

Although e(y)1190 heterozygous flies do not show any visible wing defects, e(y)1190/+ greatly enhances the wing margin notching phenotype of N264-39/+ in both expressivity and penetrance.

The germarium cap cell loss caused by N264-39/+ is significantly enhanced by enok1/+.

Scer\GAL4Bx-MS1096-mediated expression of NdfipScer\UAS.T:Ivir\HA1 suppresses the N264-39/+ wing phenotypes.

Scer\GAL4Bx-MS1096-mediated expression of NdfipKK108770 enhances the wing margin notching and slight vein thickening near the wing blade seen in N264-39/+ wings.

The severity of the notched wing phenotype seen in N264-39/+ flies is enhanced by Acn27/+ and by Acn1/+.

Heterozygosity for N264-39 suppresses the over-representation phenotype of Utx1 mosaics.

Heterozygous crb11A22 suppresses the N264-39/+ small eye phenotype. The suppression is achieved without altering the size of the facet lenses.

Heterozygosity for N264-39 suppresses the overgrowth of the adult eye and head that occurs in animals with somatic clones of Vps25N55 generated in the eye-antennal disc.

Heterozygosity for N264-39 does not suppresses the rough-eye phenotype that occurs in animals with somatic clones of Vps36L5212 generated in the eye-antennal disc.

Heterozygosity for N264-39 suppresses the overgrowth of the adult eye and head that occurs in animals with somatic clones of lsn5F3-8 generated in the eye-antennal disc.

The eye overgrowth phenotype seen in Uba1D6 mosaics raised at 29[o]C is dominantly suppressed by N264-39.

A heterozygous auxI670K background increases the severity of both the penetrance and the phenotype of N264-39 heterozygous mutants.

The wing notching phenotype seen in N264-39/+ flies is enhanced by expression of Pi3K68DKD.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL469B, but is not enhanced by expression of Pi3K68DScer\UAS.T:Hsap\MYC under the control of Scer\GAL469B.

Df(3L)vin3/+ enhances the wing notching phenotype seen in N264-39/+ flies.

Df(3R)Pi3K92EA/+ has no effect on the wing notching phenotype seen in N264-39/+ flies.

The nicked wing phenotype seen in N264-39/+ flies is suppressed by Nedd4T119FS/+.

Dominantly enhances the eye-polarity phenotype of fzhs.sev; most fzhs.sev ommatidia show R3/R3-type symmetry in a N264-39 heterozygous background. Dominantly enhances the eye-polarity phenotype of dshhs.sev.B.

Xenogenetic Interactions
Statement
Reference

A N264-39 heterozygous background suppresses the eye deformities, eye curvature, patterning of the photoreceptor nuclei later, and phototactic behavior seen in flies expressing Hsap\CHMP2BIntron5.Scer\UAS.N.T:Zzzz\FLAG under the control of Scer\GAL4ey.PH.

Complementation and Rescue Data
Rescued by
Partially rescued by
Comments

Expression of N3.hs.T:Scer\GAL4,T:Hsim\VP16 using three 40 minute heat shocks starting at 8-9 hours after egg laying does not rescue hub cell number in the gonads of male N264-39 larvae. However, expression of N3.hs.T:Scer\GAL4,T:Hsim\VP16 using three 40 minute heat shocks starting at 5-6 hours after egg laying results in significant rescue of hub cell number in the gonads of male N264-39 larvae, with almost half of the animals reaching the wild-type range of hub cells.

Expression of NScer\UAS.T:Avic\GFP under the control of Scer\GAL4prd.RG1 greatly reduces the amount of neuronal cells in N264-39 mutants.

Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer

Slizynska, 1937.

Arose on: wch.

Comments
Comments

Originally reported as a deficiency of band 3C7 (FBrf0004288). Later reexamination of chromosomes of males from lines carrying wch and marked N264-39 revealed the presence of 3C7 (Welshons)

Cytological examination reveals that this mutant chromosome is no longer cytologically deficient.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (7)
References (43)