FlyBase Allele Report: Dmel\Ras85D[e2F]

The adult eyes of Ras85D^2F4/Ras85D^e2F transheterozygotes have some clusters that are missing one or more photoreceptors. Misrotation of clusters is not seen.

(Strutt and Strutt, 2003)

Homozygous somatic clones in the legs and wings have bractless mechanosensory bristles.

(del Alamo et al., 2002)

Ras85D^C33/Ras85D^e2F survive to the third larval instar stage.

(Kramer et al., 1999)

94% of eggs laid by Ras85D^e2F/Ras85D^ix12a females have one fused dorsal appendage.

(Lu and Li, 1999)

Homozygous embryos show a loss of 3 chordotonal organs per abdominal hemisegment (VChA and two in LCh5).

(Okabe and Okano, 1997)

Embryos show reduced number of midline glial cells.

(Scholz et al., 1997, Scholz et al., 1997)

Heterozygous loss of Ras85D has no effect on size of Nf1 mutant pupae.

(The et al., 1997)

Removal of one copy of dos from sev::tor^{13D:Y2546F.hs.sev} individuals causes a significant reduction in ommatidia with multiple R7 cells.

(Raabe et al., 1996)

Ras85D^e2F/Ras85D⁰⁵⁷⁰³ transheterozygotes are semi-lethal. Female transheterozygotes with Ras85D⁰⁵⁷⁰³ lay eggs with a severely disrupted eggshell (patch of chorion instead of dorsal appendages), 57% of eggs hatch and develop to normal larvae. Unhatched embryos have normal cuticles, or are missing elements of the head skeleton or have defects in the anterior and posterior termini, holes in the cuticle and disordered posterior segments. Transheterozygotes with Ras85D⁰⁵⁷⁰³ exhibit slightly disrupted ommatidial array (small number of fused and misplaced ommatidia).

(Schnorr and Berg, 1996)

Clonal analysis reveals phenotypes in the adult including loss of wing vein, ectopic wing vein, reduced cell size, extra bristles, cell lethality and (incompletely expressed) tergite bristle abnormalities.

(Diaz-Benjumea and Hafen, 1994)

Reduces the ability of a cell to develop as an R7 cell or as any other photoreceptor.

(Simon et al., 1991)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Ras85D^e2F is an enhancer of abnormal cell polarity phenotype of S^48-5

(Gaengel and Mlodzik, 2003)

Ras85D^e2F is an enhancer of visible phenotype of BacA\p35^GMR.PH, Dp^GMR.PD, E2f1^GMR.PD

(Staehling-Hampton et al., 1999)

Ras85D^e2F is an enhancer of visible phenotype of rux³

(Thomas et al., 1994)

NOT Enhancer of

Statement

Reference

Ras85D^e2F is a non-enhancer of visible phenotype of Scer\GAL4^GMR.PF, btl^λ.UAS, stumps^UAS.Tag:FLAG

(Zhu et al., 2005)

Ras85D^e2F is a non-enhancer of visible phenotype of Pp2B-14D^act.GMR

(Sullivan and Rubin, 2002)

Ras85D^e2F is a non-enhancer of visible phenotype of Rala^S25N.UAS, Scer\GAL4^sca-537.4

(Sawamoto et al., 1999)

Suppressor of

Statement

Reference

Ras85D^e2F/Ras85D[+] is a suppressor | partially of visible | adult stage phenotype of Lztr1^GD4339, Scer\GAL4^Act5C.PI

(Bigenzahn et al., 2018)

Ras85D^e2F/Ras85D[+] is a suppressor | partially of visible | adult stage phenotype of Lztr1^HMS00306, Scer\GAL4^Act5C.PI

(Bigenzahn et al., 2018)

Ras85D^e2F/Ras85D[+] is a suppressor of increased cell number | third instar larval stage phenotype of Graf¹

(Kim et al., 2017)

Ras85D^e2F/Ras85D[+], Scer\GAL4^Act.PU is a suppressor of visible phenotype of Rabex-5^VDRC.cUa, Scer\GAL4^Act.PU

(Yan et al., 2010)

Ras85D^e2F/Ras85D[+] is a suppressor of lethal phenotype of Hsap\FOXO1::Hsap\PAX7^UAS.cGa, Scer\GAL4^Mhc.PW

(Galindo et al., 2006)

Ras85D^e2F is a suppressor of abnormal cell polarity phenotype of aos^rlt

(Gaengel and Mlodzik, 2003)

Ras85D^e2F is a suppressor of visible phenotype of Scer\GAL4^sca-109-68, ato^UAS.cJa

(White and Jarman, 2000)

Ras85D^e2F is a suppressor of visible phenotype of Scer\GAL4^Bx-MS1096, htl::tor^4021.UAS

(Feldmann et al., 1999)

Ras85D^e2F is a suppressor of lethal | heat sensitive phenotype of Src64B^{Δ540.hs.2sev}

(Kussick et al., 1993)

NOT Suppressor of

Statement

Reference

Ras85D^e2F is a non-suppressor of visible phenotype of Scer\GAL4^GMR.PF, btl^λ.UAS, stumps^UAS.Tag:FLAG

(Zhu et al., 2005)

Ras85D^e2F is a non-suppressor of visible phenotype of Pp2B-14D^act.GMR

(Sullivan and Rubin, 2002)

Ras85D^e2F is a non-suppressor of visible phenotype of Rala^S25N.UAS, Scer\GAL4^sca-537.4

(Sawamoto et al., 1999)

Phenotype Manifest In

Suppressed by

Statement

Reference

Ras85D^e2F has pericardial cell primordium | embryonic stage 10 phenotype, suppressible by Df(3R)Espl22

(Helman et al., 2011)

Ras85D^e2F/Ras85D^ix12a has dorsal appendage phenotype, suppressible | partially by Df(2R)nap8/+

(Lu and Li, 1999)

Ras85D^e2F/Ras85D^ix12a has dorsal appendage phenotype, suppressible | partially by Src42A[+]/Src42A^15-1

(Lu and Li, 1999)

Enhancer of

Statement

Reference

Ras85D^e2F is an enhancer of ommatidium phenotype of S^48-5

(Gaengel and Mlodzik, 2003)

Ras85D^e2F is an enhancer of photoreceptor cell R1 phenotype of B-H1^sev.PH

(Hayashi et al., 1998)

Ras85D^e2F is an enhancer of photoreceptor cell R2 phenotype of B-H1^sev.PH

(Hayashi et al., 1998)

Ras85D^e2F is an enhancer of photoreceptor cell R3 phenotype of B-H1^sev.PH

(Hayashi et al., 1998)

Ras85D^e2F is an enhancer of photoreceptor cell R4 phenotype of B-H1^sev.PH

(Hayashi et al., 1998)

Ras85D^e2F is an enhancer of photoreceptor cell R5 phenotype of B-H1^sev.PH

(Hayashi et al., 1998)

Ras85D^e2F is an enhancer of photoreceptor cell R6 phenotype of B-H1^sev.PH

(Hayashi et al., 1998)

Ras85D^e2F is an enhancer of eye phenotype of rpr^GMR.PW

(Kurada and White, 1998)

Ras85D^e2F is an enhancer of eye phenotype of hid^GMR.PG

(Kurada and White, 1998)

Ras85D^e2F is an enhancer of eye phenotype of rux³

(Thomas et al., 1994)

NOT Enhancer of

Statement

Reference

Ras85D^e2F is a non-enhancer of eye phenotype of Scer\GAL4^GMR.PF, btl^λ.UAS, stumps^UAS.Tag:FLAG

(Zhu et al., 2005)

Ras85D^e2F is a non-enhancer of eye phenotype of Pp2B-14D^act.GMR

(Sullivan and Rubin, 2002)

Ras85D^e2F is a non-enhancer of phenotype of Doa⁷/Doa^HD

(Yun et al., 2000)

Ras85D^e2F is a non-enhancer of phenotype of Doa^HD/Doa^Dem

(Yun et al., 2000)

Ras85D^e2F is a non-enhancer of wing phenotype of Scer\GAL4^Bx-MS1096, vap^UAS.Tag:MYC

(Feldmann et al., 1999)

Suppressor of

Statement

Reference

Ras85D^e2F/Ras85D[+] is a suppressor | partially of wing vein | ectopic phenotype of Lztr1^GD4339, Scer\GAL4^Act5C.PI

(Bigenzahn et al., 2018)

Ras85D^e2F/Ras85D[+] is a suppressor | partially of wing vein | ectopic phenotype of Lztr1^HMS00306, Scer\GAL4^Act5C.PI

(Bigenzahn et al., 2018)

Ras85D^e2F/Ras85D[+] is a suppressor of hemocyte | increased number | third instar larval stage phenotype of Graf¹

(Kim et al., 2017)

Ras85D^e2F/Ras85D[+] is a suppressor of wing vein | ectopic phenotype of sl²

(Murillo-Maldonado et al., 2011)

Ras85D^e2F/Ras85D[+] is a suppressor of eye | somatic clone phenotype of gang-of-four¹

(Beam and Moberg, 2010)

Ras85D^e2F/Ras85D[+] is a suppressor of posterior crossvein phenotype of Rabex-5^VDRC.cUa, Scer\GAL4^Act.PU

(Yan et al., 2010)

Ras85D^e2F/Ras85D[+] is a suppressor of wing vein L2 phenotype of Rabex-5^VDRC.cUa, Scer\GAL4^Act.PU

(Yan et al., 2010)

Ras85D^e2F/Ras85D[+] is a suppressor of larval somatic muscle cell phenotype of Hsap\FOXO1::Hsap\PAX7^UAS.cGa, Scer\GAL4^Mhc.PW

(Galindo et al., 2006)

Ras85D^e2F/Ras85D[+] is a suppressor of larval muscle system phenotype of Hsap\FOXO1::Hsap\PAX7^UAS.cGa, Scer\GAL4^Mhc.PW

(Galindo et al., 2006)

Ras85D^e2F is a suppressor of ommatidium phenotype of aos^rlt

(Gaengel and Mlodzik, 2003)

Ras85D^e2F is a suppressor of eye phenotype of Scer\GAL4^sca-109-68, ato^UAS.cJa

(White and Jarman, 2000)

Ras85D^e2F is a suppressor of wing vein phenotype of Scer\GAL4^Bx-MS1096, htl::tor^4021.UAS

(Feldmann et al., 1999)

Ras85D^e2F/Ras85D[+] is a suppressor of phenotype of Src42A^Su(Raf)1-1

(Zhang et al., 1999)

Ras85D^e2F is a suppressor of photoreceptor cell R1 | ectopic phenotype of B-H1^sev.PH

(Hayashi et al., 1998)

Ras85D^e2F is a suppressor of photoreceptor cell R2 | ectopic phenotype of B-H1^sev.PH

(Hayashi et al., 1998)

Ras85D^e2F is a suppressor of photoreceptor cell R3 | ectopic phenotype of B-H1^sev.PH

(Hayashi et al., 1998)

Ras85D^e2F is a suppressor of photoreceptor cell R4 | ectopic phenotype of B-H1^sev.PH

(Hayashi et al., 1998)

Ras85D^e2F is a suppressor of photoreceptor cell R5 | ectopic phenotype of B-H1^sev.PH

(Hayashi et al., 1998)

Ras85D^e2F is a suppressor of photoreceptor cell R6 | ectopic phenotype of B-H1^sev.PH

(Hayashi et al., 1998)

Ras85D^e2F/Ras85D[+] is a suppressor of phenotype of aos²⁵⁷

(Sawamoto et al., 1996)

Ras85D^e2F is a suppressor of phenotype of Src42A^KR.hs.2sev

(Takahashi et al., 1996)

Ras85D^e2F is a suppressor of phenotype of svp^1.sev

(Begemann et al., 1995)

Ras85D^e2F is a suppressor of phenotype of svp^2.sev

(Begemann et al., 1995, Kramer et al., 1995)

Ras85D^e2F is a suppressor of phenotype of rho^hs.sev

(Freeman, 1994)

Ras85D^e2F is a suppressor of eye phenotype of Src64B^{Δ540.hs.2sev}

(Kussick et al., 1993)

NOT Suppressor of

Statement

Reference

Ras85D^e2F is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PF, btl^λ.UAS, stumps^UAS.Tag:FLAG

(Zhu et al., 2005)

Ras85D^e2F is a non-suppressor of eye phenotype of Pp2B-14D^act.GMR

(Sullivan and Rubin, 2002)

Ras85D^e2F is a non-suppressor of ommatidium phenotype of Rac1^V12.hs.sev

(Fanto et al., 2000)

Ras85D^e2F is a non-suppressor of phenotype of Doa⁷/Doa^HD

(Yun et al., 2000)

Ras85D^e2F is a non-suppressor of phenotype of Doa^HD/Doa^Dem

(Yun et al., 2000)

Ras85D^e2F is a non-suppressor of phenotype of dsh^hs.sev.B

(Boutros et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

The increased concentration of circulating hemocytes characteristic for Graf¹/Y mutant third instar larvae is suppressible by combination with a single copy of Ras85D^e2F.

(Kim et al., 2017)

The loss of eve-positive pericardial progenitor cell clusters which is seen in stage 10 Ras85D^e2F embryos is partially rescued if the embryos are also mutant for Df(3R)Espl22.

(Helman et al., 2011)

One copy of Ras85D^e2F partially suppresses the ectopic wing vein phenotype seen in sl² homozygotes.

(Murillo-Maldonado et al., 2011)

The ommatidial patterning defects seen in homozygous gfr¹ clones in the pupal eye disc are not suppressed by Ras85D^e2F.

The enlarged eye phenotype seen in mosaic animals in which the eye is homozygous for gfr¹ is suppressed by Ras85D^e2F/+.

(Beam and Moberg, 2010)

Ras85D^e2F dominantly suppresses the extra wing veins caused by constitutive expression of Rabex-5^VDRC.cUa via Scer\GAL4^Act.PU.

(Yan et al., 2010)

S^48-5/Ras85D^e2F mutants show 58.05%+-4.57 misrotated ommatidia compared to 9.55%+-1.43 seen in S^48-5 mutants alone.

(Gaengel and Mlodzik, 2003)

The wing phenotype caused by expression of vap^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^Bx-MS1096 is not enhanced by one copy of Ras85D^e2F. Suppresses the extra wing vein phenotype caused by htl::tor^{4021.Scer\UAS} expressed under the control of Scer\GAL4^Bx-MS1096.

(Feldmann et al., 1999)

The fused dorsal appendage phenotype is partially suppressed by one copy of Src42A^15-1 or Df(2R)nap8.

(Lu and Li, 1999)

Enhances the phenotype of E2f^GMR.PD Dp^GMR.PD BacA\p35^GMR.PH flies.

(Staehling-Hampton et al., 1999)

Dominantly suppresses the ability of Src42A^Su(phl)1-1 to suppress the lethality of phl¹/Y flies.

(Zhang et al., 1999)

Ras85D^e2F significantly suppresses the formation of extra outer photoreceptors caused by B-H1^sev.PH. The fraction of B-H1^sev.PH ommatidia that lack 1 or 2 outer photoreceptors is increased by Ras85D^e2F.

(Hayashi et al., 1998)

Enhances the eye reduction phenotype caused by rpr^GMR.PW. Enhances the eye reduction phenotype caused by W^GMR.PG.

(Kurada and White, 1998)

fs(1)K10¹/fs(1)K10¹; Ras85D^ix13b/Ras85D^e2F females lay eggs that range from strongly dorsalised to weakly ventralised, indistinguishable from Ras85D mutant eggs. The embryos range from strongly dorsalised to weakly dorsalised, to almost normal with slight head defects.

(Schnorr and Berg, 1996)

Suppressor of the dosage-dependent (two or more copies of P{sev-svp1} or P{sev-svp2}) transformation of cone cells into R7 photoreceptors and at a lower frequency R7 cells into outer photoreceptors.

(Begemann et al., 1995)

Mutation has no effect on the rough eye phenotype caused by two insertions of P{GMR-Rho1}.

(Hariharan et al., 1995)

Suppression of the rough eye phenotype (formation of extra R7 cells) caused by P{sev-svp2} and no effect on the P{ro-svp1} rough eye phenotype (loss of one or more outer photoreceptors from many of the ommatidia).

(Kramer et al., 1995)

Enhances the sev^B4 phenotype: disrupts sev^B4 protein signalling.

(Simon et al., 1991)

Xenogenetic Interactions

Statement

Reference

Heterozygosity for Ras85D^e2F, Ras85D^e1B or Ras85D⁰⁶⁶⁷⁷ suppresses the larval muscle phenotype seen in animals expressing Hsap\FOXO1A::Hsap\PAX7^Scer\UAS.cGa under the control of Scer\GAL4^Mhc.PW and also results in viable adult escapers. Of these three alleles, Ras85D^e2F is the strongest suppressor: at the lower temperature of 23 ^oC, 83% of Hsap\FOXO1A::Hsap\PAX7^Scer\UAS.cGa, Scer\GAL4^Mhc.PW, Ras85D^e2F/+ larvae are rescued to adult viability whereas only 15% of Hsap\FOXO1A::Hsap\PAX7^Scer\UAS.cGa, Scer\GAL4^Mhc.PW larvae survive to adulthood.

(Galindo et al., 2006)

Complementation and Rescue Data

Rescued by

Ras85D^e2F/Ras85D^e1B is rescued by Ras85D^+t12

(Simon et al., 1991)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

5690

sev¹⁴; Ras85D^e2F/TM3, Sb¹

Kyoto

108367

sev¹⁴; Ras85D^e2F/TM3, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (18)

Reported As

Symbol Synonym

Dras1^e2f

(Kurada and White, 1998, Yagi et al., 1998)

Dras^e2F

(del Alamo et al., 2002)

E(sev)3C^E2F

(Boulton et al., 2000, Staehling-Hampton et al., 1999)

E(sev)3C^e2F

(Doyle and Bishop, 1993, Simon et al., 1991)

Ras-e2F

(Gaengel and Mlodzik, 2003)

Ras1^D38N

(Lu and Li, 1999, Zhang et al., 1999, Mantrova and Hsu, 1998)

Ras1^e2f

(Zhu et al., 2005, Therrien et al., 2000, White and Jarman, 2000)

Ras85D^D38N

(Schnorr and Berg, 1996)

Ras85D^e2F

(Tong et al., 2007, Galindo et al., 2006)

Ras85D^e2f

(Kim et al., 2017, Murillo-Maldonado et al., 2011, Beam and Moberg, 2010, Hasson et al., 2005, Strutt and Strutt, 2003, Sullivan and Rubin, 2002)

Ras^e2F

(Bigenzahn et al., 2018, Hannan et al., 2006)

Ras^e2f

(Yan et al., 2010)

ras1^e2F

(del Alamo et al., 2002, Scholz et al., 1997, Scholz et al., 1997, Begemann et al., 1995)

ras1^e2f

(Gafuik and Steller, 2011, Helman et al., 2011, Boutros et al., 1998)

ras^D38N

(Wu et al., 2009)

ras^E2f

(Galindo et al., 2006)

ras^e2F

(Yun et al., 2000)

Name Synonyms

Secondary FlyBase IDs

References (61)

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