Open Close
General Information
Symbol
Dmel\cactE10
Species
D. melanogaster
Name
FlyBase ID
FBal0030693
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

A small deletion derived from imprecise excision of a P-element removes part of the second exon including the initiator codon for translation and creates an additional exon from the P-element. An upstream portion of the second exon is spliced out upstream of codon 69. The ATG at position 145 is the first possible initiator resulting in an N-terminally truncated protein lacking the first 144 amino acids.

Insertion components
P{}cactE10
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The mean circulating hemocyte number of both cactE10/cact3 and cactE10/cactE8 mutants is not statistically different from that of controls.

Eggs laid by cactE10/+ females are wild type.

cactE10/cactE8 larvae and adults do not have melanotic capsules. Hemocyte density in cactE8/cactE10 hemolymph is not significantly different than in the hemolymph of control larvae.

Causes a dorsalised phenotype.

Females homozygous for this allele or heterozygous for this allele and a deficiency for cact generate strongly dorsalized or dorsolateralized embryos. Females transheterozygous for this allele and a loss of function allele result in embryos in which ventral structures are progressively lost at the expense of dorsal structures as the strength of the loss of function allele increases.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

cactE10 has lethal | maternal effect phenotype, enhanceable by fus1/fus[+]

Suppressor of
Statement
Reference
Other
Phenotype Manifest In
Enhanced by
Statement
Reference

cactE10 has embryonic epidermis phenotype, enhanceable by fus1/fus[+]

cactE10 has mesoderm phenotype, enhanceable by fus1/fus[+]

cactE10 has embryonic epidermis phenotype, enhanceable by Egfr[+]/fus[+]/Egfrfus/fus2

cactE10 has embryonic epidermis phenotype, enhanceable by dl[+]/dl1

cactE10 has embryonic epidermis phenotype, enhanceable by ea1/ea[+]

cactE10 has embryonic epidermis phenotype, enhanceable by grk3/grk3/grk[+]

cactE10 has embryonic epidermis phenotype, enhanceable by grk6/grk6/grk[+]

cactE10 has embryonic epidermis phenotype, enhanceable by spz[+]/spz4

cactE10 has embryonic epidermis phenotype, enhanceable by fus1

NOT Enhanced by
Statement
Reference

cactE10 has embryonic epidermis phenotype, non-enhanceable by fus[+]/fus2

cactE10 has embryonic epidermis phenotype, non-enhanceable by Egfr[+]/Egfrt1

Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference

cactE8/cactE10 is a non-suppressor of lamellocyte | increased number phenotype of lwr5/lwr4-3

Additional Comments
Genetic Interactions
Statement
Reference

A cactE10 background suppresses blood cell infiltration and tumorigenesis in lwr4-3/lwr5 mutants upon L.victoriae parasitic wasp infection.

The melanotic tumour penetrance is reduced in lwr4-3 cactE8/lwr5 cactE10 double mutants compared to lwr4-3/lwr5 single mutants and by day 8, the amount of circulating hemocytes is reduced in the double mutants. In contrast, the amount of lamellocytes remains as high as in the single mutants.

Embryos laid by cactE10/+, dl1/+ females are weakly dorsalized, 90% of embryos produced by cactE10/+, spz4/+ are weakly dorsalized and 50% of embryos produced by cactE10/+, ea1/+ are weakly dorsalized. More than 80% of embryos produced by cactE10/+, fus1/+ are distinctly dorsalized, and the Egfrfus,fus2 chromosome has a similar but stronger effect. Fewer than 10% of embryos produced by cactE10/+, fus1/+ females hatch and 80-90% show the twisted body and head defects characteristic of a loss of mesoderm. Mesodermal cell types are lost.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

In trans to dl amorphic alleles, this allele results in dorsalized embryos.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (18)