FlyBase Allele Report: Dmel\cact[E10]

General Information

Symbol

Dmel\cact^E10

Species

D. melanogaster

Name

FlyBase ID

FBal0030693

Feature type

allele

Associated gene

Dmel\cact

Associated Insertion(s)

P{}cact^E10

Carried in Construct

Key Links

Allele class

gain of function allele

Mutagen

PM hybrid dysgenesis

Nature of the Allele

Allele class

gain of function allele

(Bergmann et al., 1996, Geisler et al., 1995, Govind et al., 1993, Whalen and Steward, 1993, Roth et al., 1991, Ashburner et al., 1990)

Mutagen

PM hybrid dysgenesis

(Ashburner et al., 1999.10.12, Roth et al., 1991)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Cytology

Nature of the lesion

Statement

Reference

A small deletion derived from imprecise excision of a P-element removes part of the second exon including the initiator codon for translation and creates an additional exon from the P-element. An upstream portion of the second exon is spliced out upstream of codon 69. The ATG at position 145 is the first possible initiator resulting in an N-terminally truncated protein lacking the first 144 amino acids.

(Bergmann et al., 1996)

Insertion components

P{}cact^E10

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates hematologic cancer

modeled by lwr^4-3, lwr⁵

(Paddibhatla et al., 2010)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | maternal effect

(Ashburner et al., 1990)

lethal | recessive

(Roth et al., 1991)

Phenotype Manifest In

embryonic/first instar larval cuticle

(Bergmann et al., 1996)

embryonic ventral epidermis

(Govind et al., 1993, Roth et al., 1991)

Detailed Description

Statement

Reference

The mean circulating hemocyte number of both cact^E10/cact³ and cact^E10/cact^E8 mutants is not statistically different from that of controls.

(Chiu et al., 2005)

Eggs laid by cact^E10/+ females are wild type.

(Wakabayashi-Ito et al., 2001)

cact^E10/cact^E8 larvae and adults do not have melanotic capsules. Hemocyte density in cact^E8/cact^E10 hemolymph is not significantly different than in the hemolymph of control larvae.

(Qiu et al., 1998)

Causes a dorsalised phenotype.

(Bergmann et al., 1996)

Females homozygous for this allele or heterozygous for this allele and a deficiency for cact generate strongly dorsalized or dorsolateralized embryos. Females transheterozygous for this allele and a loss of function allele result in embryos in which ventral structures are progressively lost at the expense of dorsal structures as the strength of the loss of function allele increases.

(Govind et al., 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

cact^E10 has lethal | maternal effect phenotype, enhanceable by fus¹/fus[+]

(Wakabayashi-Ito et al., 2001)

Suppressor of

Statement

Reference

cact^E10 is a suppressor of abnormal immune response phenotype of lwr⁵/lwr^4-3

(Paddibhatla et al., 2010)

cact^E10 is a suppressor of neoplasia phenotype of lwr⁵/lwr^4-3

(Paddibhatla et al., 2010)

cact^E8/cact^E10 is a suppressor | partially of melanotic mass phenotype | larval stage phenotype of lwr⁵/lwr^4-3

(Chiu et al., 2005)

Other

Statement

Reference

cact^E10, lwr⁵/lwr^4-3 has lethal | wandering third instar larval stage phenotype

(Paddibhatla et al., 2010)

cact^E10, dl[-]/+ has female sterile phenotype

(Ashburner et al., 1990)

Phenotype Manifest In

Enhanced by

Statement

Reference

cact^E10 has embryonic epidermis phenotype, enhanceable by fus¹/fus[+]

(Wakabayashi-Ito et al., 2001)

cact^E10 has mesoderm phenotype, enhanceable by fus¹/fus[+]

(Wakabayashi-Ito et al., 2001)

cact^E10 has embryonic epidermis phenotype, enhanceable by Egfr[+]/fus[+]/Egfr^fus/fus²

(Wakabayashi-Ito et al., 2001)

cact^E10 has embryonic epidermis phenotype, enhanceable by dl[+]/dl¹

(Wakabayashi-Ito et al., 2001)

cact^E10 has embryonic epidermis phenotype, enhanceable by ea¹/ea[+]

(Wakabayashi-Ito et al., 2001)

cact^E10 has embryonic epidermis phenotype, enhanceable by grk³/grk³/grk[+]

(Wakabayashi-Ito et al., 2001)

cact^E10 has embryonic epidermis phenotype, enhanceable by grk⁶/grk⁶/grk[+]

(Wakabayashi-Ito et al., 2001)

cact^E10 has embryonic epidermis phenotype, enhanceable by spz[+]/spz⁴

(Wakabayashi-Ito et al., 2001)

cact^E10, fus[+]/fus² has embryonic epidermis phenotype, enhanceable by Egfr^fus

(Wakabayashi-Ito et al., 2001)

cact^E10 has embryonic epidermis phenotype, enhanceable by fus²

(Wakabayashi-Ito and Anderson, 1998)

cact^E10 has embryonic epidermis phenotype, enhanceable by fus¹

(Ito et al., 1997)

NOT Enhanced by

Statement

Reference

cact^E10 has embryonic epidermis phenotype, non-enhanceable by fus[+]/fus²

(Wakabayashi-Ito et al., 2001)

cact^E10 has embryonic epidermis phenotype, non-enhanceable by Egfr[+]/Egfr^t1

(Wakabayashi-Ito et al., 2001)

cact^E10 has embryonic epidermis phenotype, non-enhanceable by sqd^unspecified/sqd[+]

(Wakabayashi-Ito et al., 2001)

Suppressor of

Statement

Reference

cact^E10 is a suppressor of embryonic/larval fat body phenotype of lwr⁵/lwr^4-3

(Paddibhatla et al., 2010)

cact^E10 is a suppressor of embryonic/larval hemocoel phenotype of lwr⁵/lwr^4-3

(Paddibhatla et al., 2010)

cact^E8/cact^E10 is a suppressor of hemocyte | increased number phenotype of lwr⁵/lwr^4-3

(Chiu et al., 2005)

NOT Suppressor of

Statement

Reference

cact^E8/cact^E10 is a non-suppressor of lamellocyte | increased number phenotype of lwr⁵/lwr^4-3

(Chiu et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

A cact^E10 background suppresses blood cell infiltration and tumorigenesis in lwr^4-3/lwr⁵ mutants upon L.victoriae parasitic wasp infection.

(Paddibhatla et al., 2010)

The melanotic tumour penetrance is reduced in lwr^4-3 cact^E8/lwr⁵ cact^E10 double mutants compared to lwr^4-3/lwr⁵ single mutants and by day 8, the amount of circulating hemocytes is reduced in the double mutants. In contrast, the amount of lamellocytes remains as high as in the single mutants.

(Chiu et al., 2005)

Embryos laid by cact^E10/+, dl¹/+ females are weakly dorsalized, 90% of embryos produced by cact^E10/+, spz⁴/+ are weakly dorsalized and 50% of embryos produced by cact^E10/+, ea¹/+ are weakly dorsalized. More than 80% of embryos produced by cact^E10/+, fus¹/+ are distinctly dorsalized, and the Egfr^fus,fus² chromosome has a similar but stronger effect. Fewer than 10% of embryos produced by cact^E10/+, fus¹/+ females hatch and 80-90% show the twisted body and head defects characteristic of a loss of mesoderm. Mesodermal cell types are lost.