The mean circulating hemocyte number of cactE10/cactE8 mutants is not statistically different from that of controls.
The number of circulating hemocytes is increased in cactE8/cact9 third instar larvae compared to wild-type.
The ability of cactE8/cact3 larvae to encapsulate L.boulardi eggs is significantly increased compared to that of control larvae.
cactE8/cact3 larvae are viable at 24 and 48 hours after fertilisation. By 72 hours after fertilisation there is some lethality and delay in the rate of development compared to control siblings. Only 40% of the larvae survive to 120 hours after fertilisation, and only 4% of the larvae make white prepupae. Over 90% of larvae have melanotic capsules. cactE8/cact9 larvae are viable at 24 and 48 hours after fertilisation. More than 80% of the larvae survive to 120 hours after fertilisation and progress into pupal stages. Less than 1% of these animals eclose, the majority dying as pupae. Approximately 40% of larvae have melanotic capsules. cactE10/cactE8 larvae and adults do not have melanotic capsules. Hemocyte density in cactE8/cact3 hemolymph is more than 10-fold higher than in the hemolymph of control or heterozygous larvae. There are an increased number of lamellocytes in the hemolymph compared to control larvae. The lymph glands of 5 to 8 day old animals are abnormally large and there are many more hemocytes per lobe. Hemocyte density in cactE8/cactE10 hemolymph is not significantly different than in the hemolymph of control larvae.
Strong ventralizing phenotype: lack of all dorsally and laterally derived structures and the expansion of the ventral epidermis around the entire circumference. Expansion of twi expression in terminal regions.