FlyBase Allele Report: Dmel\cact[E8]

General Information

Symbol

Dmel\cact^E8

Species

D. melanogaster

Name

FlyBase ID

FBal0030706

Feature type

allele

Associated gene

Dmel\cact

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

PM hybrid dysgenesis

Nature of the Allele

Allele class

Mutagen

PM hybrid dysgenesis

(Ashburner et al., 1999.10.12, Roth et al., 1991)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

cact²⁵⁵

(Roth et al., 1991)

Cytology

Nature of the lesion

Statement

Reference

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

The mean circulating hemocyte number of cact^E10/cact^E8 mutants is not statistically different from that of controls.

(Chiu et al., 2005)

The number of circulating hemocytes is increased in cact^E8/cact⁹ third instar larvae compared to wild-type.

(Dolezal et al., 2005)

The ability of cact^E8/cact³ larvae to encapsulate L.boulardi eggs is significantly increased compared to that of control larvae.

(Sorrentino et al., 2004)

cact^E8/cact³ larvae are viable at 24 and 48 hours after fertilisation. By 72 hours after fertilisation there is some lethality and delay in the rate of development compared to control siblings. Only 40% of the larvae survive to 120 hours after fertilisation, and only 4% of the larvae make white prepupae. Over 90% of larvae have melanotic capsules. cact^E8/cact⁹ larvae are viable at 24 and 48 hours after fertilisation. More than 80% of the larvae survive to 120 hours after fertilisation and progress into pupal stages. Less than 1% of these animals eclose, the majority dying as pupae. Approximately 40% of larvae have melanotic capsules. cact^E10/cact^E8 larvae and adults do not have melanotic capsules. Hemocyte density in cact^E8/cact³ hemolymph is more than 10-fold higher than in the hemolymph of control or heterozygous larvae. There are an increased number of lamellocytes in the hemolymph compared to control larvae. The lymph glands of 5 to 8 day old animals are abnormally large and there are many more hemocytes per lobe. Hemocyte density in cact^E8/cact^E10 hemolymph is not significantly different than in the hemolymph of control larvae.

(Qiu et al., 1998)

Strong ventralizing phenotype: lack of all dorsally and laterally derived structures and the expansion of the ventral epidermis around the entire circumference. Expansion of twi expression in terminal regions.

(Roth et al., 1991)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

cact⁹/cact^E8 has melanotic mass phenotype | third instar larval stage phenotype, suppressible | partially by Adgf-A^hs.PD

(Dolezal et al., 2005)

cact⁹/cact^E8 has lethal | pupal stage phenotype, suppressible | heat sensitive | partially by Adgf-A^hs.PD

(Dolezal et al., 2005)

cact³/cact^E8 has lethal | larval stage phenotype, suppressible | partially by Adgf-A^hs.PD

(Dolezal et al., 2005)

NOT suppressed by

Statement

Reference

cact³/cact^E8 has melanotic mass phenotype | third instar larval stage phenotype, non-suppressible by Adgf-A^hs.PD

(Dolezal et al., 2005)

Suppressor of

Statement

Reference

cact^E8/cact^E10 is a suppressor | partially of melanotic mass phenotype | larval stage phenotype of lwr⁵/lwr^4-3

(Chiu et al., 2005)

Phenotype Manifest In

Suppressed by

Statement

Reference

cact⁹/cact^E8 has melanotic mass | third instar larval stage phenotype, suppressible | partially by Adgf-A^hs.PD

(Dolezal et al., 2005)

NOT suppressed by

Statement

Reference

cact³/cact^E8 has melanotic mass | third instar larval stage phenotype, non-suppressible by Adgf-A^hs.PD

(Dolezal et al., 2005)

Suppressor of

Statement

Reference

cact^E8/cact^E10 is a suppressor of hemocyte | increased number phenotype of lwr⁵/lwr^4-3

(Chiu et al., 2005)

NOT Suppressor of

Statement

Reference

cact^E8/cact^E10 is a non-suppressor of lamellocyte | increased number phenotype of lwr⁵/lwr^4-3

(Chiu et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

The melanotic tumour penetrance is reduced in lwr^4-3 cact^E8/lwr⁵ cact^E10 double mutants compared to lwr^4-3/lwr⁵ single mutants and by day 8, the amount of circulating hemocytes is reduced in the double mutants. In contrast, the amount of lamellocytes remains as high as in the single mutants.

(Chiu et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

cact^E8

cactus^E8

(Novakova and Dolezal, 2011)

Name Synonyms

Secondary FlyBase IDs

References (7)

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