Although dac1 lamina precursor cells are able to undergo mitosis and are integrated into columns, they fail to express a late neuronal differentiation marker.
Mutant animals have almost no eye tissue, and small fused legs.
The mushroom bodies of dac1/dac3 or dac1/Df(2L)dac-4 adults show a number of defects. The α lobes are reduced in size (this phenotype is completely penetrant). Aberrant projections are often seen emanating from the calyx along the peduncle and in the direction of the tips of the α lobes. The medial (β, β' and γ) lobes appear grossly disorganised (this phenotype is completely penetrant). Aberrant projections are often seen emanating from the γ lobe and projections from the heel are occasionally seen in dac1/dac3 adults. The ellipsoid body is severely disorganised in dac1/dac3 adults. Only 3.2% of dac1/dac3 and 8.9% of dac1/Df(2L)dac-4 third instar larvae show a marked reduction in size of the α-type lobe and the defect is frequently unilateral. No aberrant projections are evident from the β-type lobe, the peduncle or the calyx.
Homozygous adults exhibit severely reduced or no eyes due to failure of the morphogenetic furrow to initiate.
Eyes of homozygotes are severely reduced and roughened, or absent. Ommatidia have either too many or too few photoreceptors, and the ommatidial array is disrupted. Morphogenetic furrow movement does not occur in about half mutant eye discs, and is dramatically reduced in the rest. Mutants have short, little legs. Femur, tibia and proximal three tarsi are severely condensed. Cell death is increased in mutant leg discs.