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General Information
Symbol
Dmel\grkHF
Species
D. melanogaster
Name
FlyBase ID
FBal0031411
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
grkHF48
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

C8432396T

Reported nucleotide change:

C1721T

Amino acid change:

Q57term | grk-PA

Reported amino acid change:

Q56term

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Nucleotide substitution: C1721T.

Amino acid replacement: Q56term.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

grkHF/+ mothers lay mildly ventralised eggs (about 54%). grkHF homozygous mothers lay 100% of eggs with strong ventralised egg shells.

Eggs derived from grk2B6/grkHF females lack operculum regions II and III (the section that normally extends from the area surrounding the micropyle to the dorsal appendages and the section that is normally between the dorsal appendages), and instead only region I cells are present symmetrically surrounding the micropyle, following a symmetric collar that separates the operculum from the rest of the chorion.

Heterozygous females lay eggs with fused or partially fused dorsal appendages (98% at 18oC, 66.7% at 25oC and 34% at 29oC).

Embryos exhibit reduction or absence of dorsal appendages (ventralization) with increase in main body egg shell. Micropyle is present at both ends of eggs, embryos are ventralized.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement
Reference

grkHF has dorsal appendage phenotype, enhanceable by CSN5L4032/CSN5[+]

NOT Enhancer of
Statement
Reference

grkHF is a non-enhancer of denticle belt phenotype of Krn27, spi1, vnL6

Other
Additional Comments
Genetic Interactions
Statement
Reference

vnL6 enhances the ventral patterning defects seen in spi1 mutant embryos. The double mutants have a reduced number of denticle belts compared to controls, fusion of the denticle belts and an anterior hole. The phenotype is further exacerbated by Krn27, with embryos having no denticle belts and a large anterior hole. A zygotic grkHF mutation has no additional effect.

Krn27 enhances the ventral patterning defects seen in spi1 mutant embryos. The double mutants have a reduced number of denticle belts compared to controls, fusion of the denticle belts and an anterior hole. The phenotype is further exacerbated by vnL6, with embryos having no denticle belts and a large anterior hole. A zygotic grkHF mutation has no additional effect.

Eggs derived from females expressing dppScer\UAS.cSa under the control of Scer\GAL4CY2 in a grk2B6/grkHF background have misshaped micropyles, show the loss of the collar that normally separates the operculum from the rest of the chorion and show a reduction in egg diameter.

Expression of grk::ylgrk1-275.yl1826-1984 is able to suppress the grk2B6/grkHF oogenesis phenotype.

grk::ylgrk1-275.yl1826-1984.

The egg chambers of Pvf1EP1624; spi1; grkHF females do not develop normally.

The fused dorsal appendage phenotype of eggs laid by grkHF/+ females is enhanced if the females are also heterozygous for CSN5L4032.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Rescued by
Comments

grkT:Arus\HRP rescues the ventralised egg shell phenotype of eggs laid by grkHF mutant mothers.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
References (19)