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General Information
Symbol
Dmel\modL8
Species
D. melanogaster
Name
FlyBase ID
FBal0032165
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
A4-4L8
Key Links
Mutagen
    Nature of the Allele
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference

    Approximately 24kb deletion with one breakpoint in the P{wA}4-4 element and the proximal breakpoint 47bp 5' of the longest krz cDNA detected.

    Breaks in 5' region of mod and deletes coding region.

    Caused by aberration
    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease-implicated variant(s)
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    Homozygous larvae reach the third larval stage with a 6-hour delay and do not exhibit significant size differences relative to wild-type. Larvae enter pupariation with a 3-day delay. Most die as pharate adult. The few escapers are reduced in body weight (26%) and total wing area (6%). The wing cell area is strongly reduced by 22%. However overall larval growth is not impaired in 5-day old larvae. Larval tissues in mutants normal. The endoreplicative cells of the larval gut are of normal size. However, mutant midguts 5 days after egg laying (AEL) has fewer isolated intestinal adult precursor cells (apc) than wild-type. They are usually seen as doublets or occasionally as groups of 3-4 cells (as opposed to 4-8 cells in wild-type). In addition mutant apc appear to be smaller than wild-type. Proliferation is not arrested however, since apc eventually form groups of 4-8 cells in 7 days AEL mutant larvae. Mutant wing imaginal discs are severely delayed in growth. However at the extended third instar (7-8 days AEL), the mutant discs attain a size comparable to prepupal wild-type discs. The reduced growth rate is not due to an enhancement of cell death, as no increase of apoptotic cells is observed. The cell cycle profile of mutant prepupal wing discs is normal, but a reduction in size in G1 and G2 cells.

    100% of modL8/mod07570 males are sterile. modL8 animals (in which the krzL8 phenotype of the Df(3R)A4-4L8 deficiency has been rescued by krz+t5.8) fail to eclose and die as pharate adults. They pupate up to 7 days after their control siblings and have a distinct minute phenotype.

    Homozygous clones in the head, thorax, legs and abdomen are reduced in size compared to control clones. Clones in the thorax produce short slender bristles. This phenotype is cell-autonomous.

    High frequency of melanotic tumours.

    Severe muscle phenotype: myoblasts retain a segmentally repeated pattern but fail to form elongated fibres and establish or maintain their insertion in the epidermis. In less severe cases myoblasts fuse and muscle fibres elongate but fail to reach the right attachment sites on the epidermis. Defects in head and tail structures. Salivary glands of mutant larva escapers exhibit gross morphological defects.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Phenotype Manifest In
    Additional Comments
    Genetic Interactions
    Statement
    Reference
    Xenogenetic Interactions
    Statement
    Reference
    Complementation and Rescue Data
    Not rescued by

    modL8 is not rescued by modt5.8

    Comments
    Images (0)
    Mutant
    Wild-type
    Stocks (1)
    Notes on Origin
    Discoverer
    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (4)
    Reported As
    Symbol Synonym
    Name Synonyms
    Secondary FlyBase IDs
      References (9)