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General Information
Symbol
Dmel\stghs.PE2
Species
D. melanogaster
Name
heat shock construct of Edgar
FlyBase ID
FBal0035720
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Hs-stg
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

stg gene expression is under the control of Hsp70 promoter.

Allele components
Product class / Tool use(s)
Regulatory region(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

There is a delay in GMC-1 entering G2-phase in embryos that are ectopically expressing stg.

stg overproduction induces mitoses in the dorsal and ventral anterior domains of the ZNC in the developing wing.

Heat induced expression causes precocious mitoses and the formation of sensory mother cells is disturbed, resulting in the loss of macrochaetes in the adult notum.

Heat shock of wandering stage third instar larvae causes cells, across all developmental compartments of the wing disk, to enter premature mitoses. Ectopically induced mitoses display the normal progression of mitotic events. Ectopic induction results in mitoses in 30% of wing disc cells. Complete mitoses occur among the bristle progenitor cells as a consequence of ectopic stg expression. The minimum heat shock time required to induce mitoses is approximately 10 minutes. Abdominal histoblasts are refractory to ectopic stg expression. Ectopic stg expression results in mitoses just posterior to the furrow in the eye disc, no mitoses are observed in the furrow itself. Induced mitoses in eye discs show normal progression of mitotic events. Heat induced third instar larvae develop into adults indistinguishable from wild-type.

Heat shock 160-185 minutes after egg deposition causes the mitotic times of all embryonic cells, except those in the amnioserosa and mitotic domain B that are normally nondividing, to advance. The length of the S phase is unaltered. Heat shocked stg proteins can only induce mitotic divisions in G2 cells. Heat shock of G2 cells of cycle 14 causes all cells, including those from the amnioserosa, to enter S phase. By varying the time of heat pulses cells can only be induced to divide very early in G2 of interphase 16.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
NOT Suppressor of
Statement
Reference
Phenotype Manifest In
Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Wing disc compartments expressing Ras85DV12.Scer\UAS, dlg1GD4689, Pi3K92EGD11228, together with CycEScer\UAS.cRa and stghs.PE2 show a rescue in compartment size and high levels of His3-positive cells.

Coexpression of the transgenes, cdc2hs.PS, CycBΔ90, and CycB3Δ, does not restore the entry of epidermal cells in CycAC8LR1 embryos into mitosis 16.

Myb2 mutant pupae carrying stghs.PE2 raised at 25oC exhibit partial suppression of the wing phenotype, the number of hairs is increased by 30-35% and the orientation of the hairs is corrected.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescues
Comments

Rescues the retinal extra photoreceptor phenotype of stghwy mutants.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
stghs.PE2
Name Synonyms
heat shock construct of Edgar
Secondary FlyBase IDs
    References (12)