Construct: P{walter} contains 12 single-base changes, each of which adds or removes a restriction site relative to the canonical w gene sequence. Construct: P{walLf} contains an insertion of an approximately 13,000bp f gene fragment (f+t13) into the polylinker of P{walL}. Construct: P{walLG} contains an insertion of approximately 4000bp DNA into the polylinker of P{walL}. Construct: P{walLK} contains an insertion of 1858bp DNA into the polylinker of P{walL}.
12 substitutions of single base pairs within a region of approximately 3kb of a w minigene.
Construct: P{walLKP} contains an insertion of a 1124bp KP element (missing the first and last 15 nucleotides) compared to P{walL}. Construct: P{walL} contains an insertion of a polylinker at position -2368 (numbering scheme from FBrf0041384) compared to P{walter}. Construct: P{walLy} contains an insertion of y+t7.7 into the polylinker of P{walL}. 12 single-base changes, each of which adds or removes a restriction site relative to the canonical w gene sequence.
Carried in plasmid "pMKw" and used in an in vitro transposition system investigating the transposition of Himar1.
Carried in plasmid "pP{walLf}" and injected into the posterior region of syncytial blastoderm whd80k17 embryos, where the pole cells will develop, for gene conversion assays using a stable genomic transposase source. Carried in plasmid "pP{walLG}" and injected into the posterior region of syncytial blastoderm whd80k17 embryos, where the pole cells will develop, for gene conversion assays using a stable genomic transposase source. Carried in plasmid "pP{walLK}" and injected into the posterior region of syncytial blastoderm whd80k17 embryos, where the pole cells will develop, for gene conversion assays using a stable genomic transposase source. Injected into the posterior region of syncytial blastoderm whd80k17 embryos, where the pole cells will develop, for gene conversion assays using a stable genomic transposase source or a coinjected transposase source. Carried in plasmid "pBSwalter" and injected into the posterior region of syncytial blastoderm whd80k17 embryos, where the pole cells will develop, for gene conversion assays using a coinjected transposase source. These assays demonstrate that P-element ends play little or no role in the conversion process from a plasmid template. Independent conversion events can occur in more than one pole cell in a single injected embryo.
Used as a template for transposase-mediated conversion of whd80k17 sequences.