FB2025_01 , released February 20, 2025
Allele: Dmel\walter
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General Information
Symbol
Dmel\walter
Species
D. melanogaster
Name
alter
FlyBase ID
FBal0035972
Feature type
allele
Associated gene
Dmel\w
Associated Insertion(s)
Carried in Construct
P{walL}
P{walLy}
P{walter}
Also Known As
P{walter}
Key Links
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Keeler and Gloor, 1997, Keeler et al., 1996, Engels et al., 1994, Nassif et al., 1994)
Progenitor genotype
w+mC
(Nassif et al., 1994)
Carried in construct
P{walL.25}
P{walL.242}
P{walL.HTT-4168}
P{walL}
P{walLf}
P{walLG}
P{walLK}
P{walLKP}
P{walLy}
P{walL-λ3Kb}
P{walL-λ8Kb}
P{walter}
Cytology
Description

Contains a w gene with 12 single-base alterations compared to the canonical w sequence together with y gene sequences.

(Keeler and Gloor, 1997)

Construct: P{walter} contains 12 single-base changes, each of which adds or removes a restriction site relative to the canonical w gene sequence. Construct: P{walLf} contains an insertion of an approximately 13,000bp f gene fragment (f+t13) into the polylinker of P{walL}. Construct: P{walLG} contains an insertion of approximately 4000bp DNA into the polylinker of P{walL}. Construct: P{walLK} contains an insertion of 1858bp DNA into the polylinker of P{walL}.

(Keeler et al., 1996)

12 substitutions of single base pairs within a region of approximately 3kb of a w minigene.

(Lankenau, 1995)

Construct: 2456bp of contiguous w locus sequence on one side of the site of P-element excision in "whd" allele series and 999bp on the other.

(Engels et al., 1994)

Construct: P{walLKP} contains an insertion of a 1124bp KP element (missing the first and last 15 nucleotides) compared to P{walL}. Construct: P{walL} contains an insertion of a polylinker at position -2368 (numbering scheme from FBrf0041384) compared to P{walter}. Construct: P{walLy} contains an insertion of y+t7.7 into the polylinker of P{walL}. 12 single-base changes, each of which adds or removes a restriction site relative to the canonical w gene sequence.

(Nassif et al., 1994)
Allele components
Component
Use(s)
Encoded product / tool
w
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Carried in plasmid "pMKw" and used in an in vitro transposition system investigating the transposition of Himar1.

      (Lampe et al., 1998)

      Carried in plasmid "pP{walLf}" and injected into the posterior region of syncytial blastoderm whd80k17 embryos, where the pole cells will develop, for gene conversion assays using a stable genomic transposase source. Carried in plasmid "pP{walLG}" and injected into the posterior region of syncytial blastoderm whd80k17 embryos, where the pole cells will develop, for gene conversion assays using a stable genomic transposase source. Carried in plasmid "pP{walLK}" and injected into the posterior region of syncytial blastoderm whd80k17 embryos, where the pole cells will develop, for gene conversion assays using a stable genomic transposase source. Injected into the posterior region of syncytial blastoderm whd80k17 embryos, where the pole cells will develop, for gene conversion assays using a stable genomic transposase source or a coinjected transposase source. Carried in plasmid "pBSwalter" and injected into the posterior region of syncytial blastoderm whd80k17 embryos, where the pole cells will develop, for gene conversion assays using a coinjected transposase source. These assays demonstrate that P-element ends play little or no role in the conversion process from a plasmid template. Independent conversion events can occur in more than one pole cell in a single injected embryo.

      (Keeler et al., 1996)

      Used as a template for transposase-mediated conversion of whd80k17 sequences.

      (Keeler and Gloor, 1997)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (10)
      Reported As
      Symbol Synonym
      w::yalLy
      walL
      walLy
      walt
      walter
      walL
      (Nassif et al., 1994)
      walLKP
      (Nassif et al., 1994)
      walLy
      (Nassif et al., 1994)
      Name Synonyms
      alter
      (Engels et al., 1994)
      walter
      (Nassif et al., 1994)
      Secondary FlyBase IDs
      • FBal0050894
      • FBal0050927
      References (11)