FB2025_01 , released February 20, 2025
Allele: Dmel\walter
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General Information
Symbol
Dmel\walter
Species
D. melanogaster
Name
alter
FlyBase ID
FBal0035972
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
P{walter}
Key Links
Transgenic product class
Nature of the Allele
Transgenic product class
Progenitor genotype
Cytology
Description

Contains a w gene with 12 single-base alterations compared to the canonical w sequence together with y gene sequences.

Construct: P{walter} contains 12 single-base changes, each of which adds or removes a restriction site relative to the canonical w gene sequence. Construct: P{walLf} contains an insertion of an approximately 13,000bp f gene fragment (f+t13) into the polylinker of P{walL}. Construct: P{walLG} contains an insertion of approximately 4000bp DNA into the polylinker of P{walL}. Construct: P{walLK} contains an insertion of 1858bp DNA into the polylinker of P{walL}.

12 substitutions of single base pairs within a region of approximately 3kb of a w minigene.

Construct: 2456bp of contiguous w locus sequence on one side of the site of P-element excision in "whd" allele series and 999bp on the other.

Construct: P{walLKP} contains an insertion of a 1124bp KP element (missing the first and last 15 nucleotides) compared to P{walL}. Construct: P{walL} contains an insertion of a polylinker at position -2368 (numbering scheme from FBrf0041384) compared to P{walter}. Construct: P{walLy} contains an insertion of y+t7.7 into the polylinker of P{walL}. 12 single-base changes, each of which adds or removes a restriction site relative to the canonical w gene sequence.

Allele components
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Expression Data
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Marker for
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Reporter construct used in assay
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Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
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Comments

Carried in plasmid "pMKw" and used in an in vitro transposition system investigating the transposition of Himar1.

Carried in plasmid "pP{walLf}" and injected into the posterior region of syncytial blastoderm whd80k17 embryos, where the pole cells will develop, for gene conversion assays using a stable genomic transposase source. Carried in plasmid "pP{walLG}" and injected into the posterior region of syncytial blastoderm whd80k17 embryos, where the pole cells will develop, for gene conversion assays using a stable genomic transposase source. Carried in plasmid "pP{walLK}" and injected into the posterior region of syncytial blastoderm whd80k17 embryos, where the pole cells will develop, for gene conversion assays using a stable genomic transposase source. Injected into the posterior region of syncytial blastoderm whd80k17 embryos, where the pole cells will develop, for gene conversion assays using a stable genomic transposase source or a coinjected transposase source. Carried in plasmid "pBSwalter" and injected into the posterior region of syncytial blastoderm whd80k17 embryos, where the pole cells will develop, for gene conversion assays using a coinjected transposase source. These assays demonstrate that P-element ends play little or no role in the conversion process from a plasmid template. Independent conversion events can occur in more than one pole cell in a single injected embryo.

Used as a template for transposase-mediated conversion of whd80k17 sequences.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (10)
Reported As
Symbol Synonym
w::yalLy
walL
walLy
walt
walter
Name Synonyms
Secondary FlyBase IDs
  • FBal0050894
  • FBal0050927
References (11)