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General Information
Symbol
Dmel\dac3
Species
D. melanogaster
Name
FlyBase ID
FBal0039229
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Mutagen
    Nature of the Allele
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference

    Contains a 25 kb insertion.

    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease-implicated variant(s)
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    dac3 neuroblast clones generated in first instar larvae contain similar numbers of neurons as wild-type controls.

    dac3 neuroblast clones induced in early larvae (to mark &ygr; neurons), in late larvae (to mark α'β' neurons) and in pupae (to mark αβ neurons) all extend axons into the proper lobes. Axons lacking dac did exhibit some irregularities, with misrouting and over-extension of projections occurring in αβ and α'β neurons. Despite their relatively normal projection pattern, axons of all three types of mushroom body neurons are more extensively and variably branched in dac3 mutant single cell clones compared to controls, particularly mutant &ygr; axons.

    In dac3 mutant first instar larval neuroblast clones the axons fail to contribute significantly to the α lobe and the medially projecting axons are unevenly distributed within the horizontal lobes. In mid-third instar larval neuroblast clones the clonal axons project primarily to the α' and β' loves, and only a few clonal axons contribute to the α and β lobes. Although axons of dac3 larval neuroblast clones fail to contribute normally to the α lobe, the axons of dac3 pupal neuroblast clones project essentially normally to the α and β lobes, with β axons occasionally crossing the midline.

    dac3 mutant αβ neurons project to α' and β' lobes in larval neuroblast clones.

    dac3 mutants have no eyes.

    Tv neurons show normal innervation of the dorsal neurohemal organs in dac3/Df(2L)dac-4 mutant embryos.

    dac3/dacGAL4 animals have almost no eyes, and those ommatidia that remain are severely disorganised. Homozygous mutant animals have eyes smaller than a third of wild-type size, and small fused legs.

    In dac3/Df(2L)dac-4 transheterozygotes a fusion of the antennal segment 5 (a5) with the arista and a reduction in width of a5 are seen. However, no obvious reductions in length or loss of segments are seen in the mutant antenna.

    Mutant animals rarely eclose due to their truncated legs. Mutant females still have two spermathecae (as in wild type) but the two ducts are fused into one branched duct that is shared by both spermathecae. Male mutant flies show a severe reduction of the clasper; the clasper is truncated, has a reduced number of clasper teeth bristles and lacks the long bristle at the "distal" end.

    Homozygous clones in the leg result in segment fusions. Homozygous clones in the leg disc are a comparable size to wild-type clones.

    The mushroom bodies of dac1/dac3 or dac3/Df(2L)dac-4 adults show a number of defects. The α lobes are reduced in size. Aberrant projections are often seen emanating from the calyx along the peduncle and in the direction of the tips of the α lobes. The medial (β, β' and γ) lobes appear grossly disorganised. Aberrant projections are often seen emanating from the γ lobe and projections from the heel are occasionally seen. The ellipsoid body is severely disorganised in dac1/dac3 adults. Only 3.2% of dac1/dac3 and 10.5% of dac3/Df(2L)dac-4 third instar larvae show a marked reduction in size of the α-type lobe and the defect is frequently unilateral. No aberrant projections are evident from the β-type lobe, the peduncle or the calyx. Homozygous mushroom body neuroblast clones generated just after hatching fail to contribute to the α lobes. This defect is not due to the death of α/β neurons. Cells of the clone do contribute to the α' lobes. In contrast to wild-type control clones, which fill the horizontal mushroom body lobes homogeneously, mutant axons fill these neuropils in a disorganised way, with a substantial variability between clones.

    Mutants lack eyes because of a failure of morphogenetic furrow initiation.

    Homozygous adults exhibit severely reduced or no eyes due to failure of the morphogenetic furrow to initiate.

    Eyes of homozygotes are severely reduced and roughened, or absent. Morphogenetic furrow movement does not occur in about half mutant eye discs, and is dramatically reduced in the rest. Mutants have short, little legs. Femur, tibia and proximal three tarsi are severely condensed. Cell death is increased in mutant leg discs.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Suppressed by
    Statement
    Reference
    Phenotype Manifest In
    Suppressed by
    Statement
    Reference
    Suppressor of
    Statement
    Reference

    dac3 is a suppressor | partially of wing phenotype of Scer\GAL4dpp.PH, eyaUAS.cHa

    Additional Comments
    Genetic Interactions
    Statement
    Reference

    The proportion of eyaScer\UAS.cHa; Scer\GAL4dpp.PH flies with blistered wings (76%) is decreased by heterozygosity for dac3 to 4%, while the proprotion with only mild wing defects is increased from 24% to 96%. (All data from experiments with a single, 'strong' P{UAS-eya.H} insertion; n=approximately 300 in each case).

    Modifies the EgfrE3 mutant eye phenotype.

    Xenogenetic Interactions
    Statement
    Reference

    Eyes are restored to almost normal morphology by dac::Ggal\Dach231::556.Scer\UAS,Scer\GAL4dac-p7d23.

    Complementation and Rescue Data
    Partially rescued by
    Not rescued by
    Comments

    dac7C1.Scer\UAS expressed under the control of Scer\GAL4OK107 rescues the mushroom body defects of dac1/Df(2L)dac-4 animals to very close to wild type; the γ lobe shows minor disorganisation. The rescue is temperature dependent (greater rescue at 25oC compared to 18oC). dac7C1.Scer\UAS expressed under the control of Scer\GAL4OK107 rescues the mushroom body defects of dac3/Df(2L)dac-4 animals to very close to wild type; the γ lobe shows minor disorganisation. dac3; eyD1Da and dac3; eyJD double heterozygotes have normal mushroom bodies and eyes.

    Scer\GAL4dpp.blk1 mediated expression of dacScer\UAS.cSa rescues eye development (fully rescues the morphogenetic furrow initiation defect) but the ommatidial array is disorganised.

    Images (0)
    Mutant
    Wild-type
    Stocks (1)
    Notes on Origin
    Discoverer
    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (2)
    References (35)