In 2XdppScer\UAS.cCa; Scer\GAL4nub-AC-62 third instar larvae, wing pouches of the wing discs are dramatically expanded in the anterior-posterior direction. This expansion is less dramatic when only on copy of dppScer\UAS.cCa is present. BrdU incorporation in these discs is concentrated laterally, in contrast to the even incorporation seen in wild-type discs. The resulting adults have enlarged wings in which anterior and posterior surfaces fail to adhere properly to each other.
Expression of dppScer\UAS.cCa under the control of Scer\GAL4cad-em459 can result in the complete duplication of the vaginal plates. However, in some cases, all the terminalia are deleted.
Flies expressing dppScer\UAS.cCa under the control of Scer\GAL4dpp.blk1 show ectopic initiation of differentiation along the anterior-ventral margin of the eye-antennal disc.
Expression of dppScer\UAS.cCa under the control of Scer\GAL4dpp.blk1 results in fusion at the tip of the claspers and lateral lobes in male external genitalia and fusion at the tip of the vaginal plates in female external genitalia.
When expression is governed by Scer\GAL4dpp.blk1, ommatidial differentiation from the posterior margin proceeds mostly normally, but precocious differentiation initiates at the anterior, and slightly ventral margin. In older discs ectopic ommatidial fields occupy the anterior ventral region of the disc. Ectopic furrows initiate near the anterior margin and progress posteriorly and inwards.
When expressed via Scer\GAL469B, tracheal tree is modified such that only dorsoventral tracheal branches arise while longitudinal branches fail to form. This phenotype is partially reversed is the embryos are also mutant for tkv.
Ectopic expression of dppScer\UAS.cCa using Scer\GAL471B does not affect vein 3, its associated sensilla and vein 4 in wing discs. Induces ectopic sensory organ precursors in the wing disc, corresponding to Sc12 and some clusters of the ventral radius, when expressed using Scer\GAL4C-765. Produces a general excess of bristles in the notum, except in the scutellum, when expressed using Scer\GAL4C-765 at 17oC. Causes the disappearance of most scutum and prescutum bristles, and does not give extra bristles in the scutellum, when expressed using Scer\GAL4C-765 or Scer\GAL4pnr-MD237 at 25oC.
Scer\GAL4MS248-mediated expression of a weakly expressed line causes reduced dorsal vaginal plates and duplicated ventral vaginal plates and no vulva. Males exhibit strong reduction of the penis apparatus. Expression of a strong line causes an extreme alteration in male genitalia, lateral plates and claspers are very reduced and fused.
When expressed from Scer\GAL4dpp.blk1, wing outgrowths occur. Ectopic wings are located postero-dorsally and mirror-symmetrically to the normal wing, as indicated by the opposing allulae.
Ectopic expression of dpp in the wing, in the Scer\GAL4 expressing line MS209 and 30A, induces overgrowth with a mirror image duplication of structures of the anterior and posterior compartments. Expression in other Scer\GAL4 expressing lines does not produce mirror image duplications. Ectopic expression in MS941 Scer\GAL4 expressing line causes defects in the anterior wing margin and posterior crossvein. Ectopic expression in MS1096 Scer\GAL4 expressing line causes severe disorganisation and necrosis of the wing.