Open Close
General Information
Symbol
Dmel\dppUAS.cCa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Capdevila
FlyBase ID
FBal0039276
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-dpp
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of a 2860bp EcoRI fragment carrying the entire dpp open reading frame.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of dppScer\UAS.cCa under the control of Scer\GAL4dpp.blk1 increases the size of the pouch in the haltere disc.

In 2XdppScer\UAS.cCa; Scer\GAL4nub-AC-62 third instar larvae, wing pouches of the wing discs are dramatically expanded in the anterior-posterior direction. This expansion is less dramatic when only on copy of dppScer\UAS.cCa is present. BrdU incorporation in these discs is concentrated laterally, in contrast to the even incorporation seen in wild-type discs. The resulting adults have enlarged wings in which anterior and posterior surfaces fail to adhere properly to each other.

Expression of dppScer\UAS.cCa under the control of Scer\GAL4cad-em459 can result in the complete duplication of the vaginal plates. However, in some cases, all the terminalia are deleted.

Flies expressing dppScer\UAS.cCa under the control of Scer\GAL4dpp.blk1 show ectopic initiation of differentiation along the anterior-ventral margin of the eye-antennal disc.

Expression of dppScer\UAS.cCa under the control of Scer\GAL4dpp.blk1 results in fusion at the tip of the claspers and lateral lobes in male external genitalia and fusion at the tip of the vaginal plates in female external genitalia.

When expression is governed by Scer\GAL4dpp.blk1, ommatidial differentiation from the posterior margin proceeds mostly normally, but precocious differentiation initiates at the anterior, and slightly ventral margin. In older discs ectopic ommatidial fields occupy the anterior ventral region of the disc. Ectopic furrows initiate near the anterior margin and progress posteriorly and inwards.

When expressed via Scer\GAL469B, tracheal tree is modified such that only dorsoventral tracheal branches arise while longitudinal branches fail to form. This phenotype is partially reversed is the embryos are also mutant for tkv.

Ectopic expression of dppScer\UAS.cCa using Scer\GAL471B does not affect vein 3, its associated sensilla and vein 4 in wing discs. Induces ectopic sensory organ precursors in the wing disc, corresponding to Sc12 and some clusters of the ventral radius, when expressed using Scer\GAL4C-765. Produces a general excess of bristles in the notum, except in the scutellum, when expressed using Scer\GAL4C-765 at 17oC. Causes the disappearance of most scutum and prescutum bristles, and does not give extra bristles in the scutellum, when expressed using Scer\GAL4C-765 or Scer\GAL4pnr-MD237 at 25oC.

Scer\GAL4MS248-mediated expression of a weakly expressed line causes reduced dorsal vaginal plates and duplicated ventral vaginal plates and no vulva. Males exhibit strong reduction of the penis apparatus. Expression of a strong line causes an extreme alteration in male genitalia, lateral plates and claspers are very reduced and fused.

When expressed from Scer\GAL4dpp.blk1, wing outgrowths occur. Ectopic wings are located postero-dorsally and mirror-symmetrically to the normal wing, as indicated by the opposing allulae.

Ectopic expression of dpp in the wing, in the Scer\GAL4 expressing line MS209 and 30A, induces overgrowth with a mirror image duplication of structures of the anterior and posterior compartments. Expression in other Scer\GAL4 expressing lines does not produce mirror image duplications. Ectopic expression in MS941 Scer\GAL4 expressing line causes defects in the anterior wing margin and posterior crossvein. Ectopic expression in MS1096 Scer\GAL4 expressing line causes severe disorganisation and necrosis of the wing.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement
Reference
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

The co-expression of dppUAS.cCa and pnutGD1512 from the second instar larval stage under the combined control of Scer\GAL4ap-md544 and Gal80[ts] does not leads to the formation of tumors in the third instar larval wing disc.

dppScer\UAS.cCa; Scer\GAL4bi-omb-Gal4 does not suppress duplication of the wing pouch and prospective notum in ds38k homozygous wing discs. However, the resulting discs have an expanded, rather than a reduced wing pouch, and anterior-posterior subdivision of the wing pouch is restored.

The ectopic initiation of differentiation along the anterior-ventral margin of the eye-antennal disc seen in flies expressing dppScer\UAS.cCa under the control of Scer\GAL4dpp.blk1 is suppressed by hhts2.

Ectopic expression of dppScer\UAS.cCa in ptcScer\UAS.cJa Scer\GAL471B flies does not restore either presumptive veins 3 and 4 or the Sc25, L3 or ACV sensilla precursors. Flies expressing ptcScer\UAS.cJa using Scer\GAL4MD-638 lack wing veins 2 and 5, the costa and alula, although wing veins 3 and 4 and the region between them are unaffected. Restores most of the wing tissue lost in ptcScer\UAS.cJa Scer\GAL4MD-638 flies when expressed using Scer\GAL4MD-638.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
dppScer\UAS.cCa
dppUAS.cCa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Capdevila
Secondary FlyBase IDs
    References (20)