Q156term | Hrs-PA; Q269term | Hrs-PB; Q269term | Hrs-PC
Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.
Somatic clones of Rbf15aΔ;HrsD28 double homozygous cells display significantly higher levels of apoptosis in third instar larval wing and eye discs, even in clones posterior to the eye disc morphogenetic furrow, compared to clones homozygous for either Rbf15aΔ or HrsD28 alone.
HrsD28 Stam2L2896 double mutant imaginal eye disc clonal cells form large clones of mesenchymal-like cells. Mosaic eye imaginal discs or eye imaginal discs consistently predominantly of cells mutant for both HrsD28 and Stam2L2896 show a loss of epithelial architecture phenotype.
Eye discs consisting predominantly of cells mutant for HrsD28 Stam2L2896 progress to form adult eyes. These are smaller than wild-type and have a rough appearance but contain some mutant photoreceptors. The scarcity of mutant adult photoreceptors might be due to cell death, as apoptotic cells are seen these mutants.
While HrsD28 homozygotes die at the early pupal stage, animals homozygous for HrsD28 and Df(1)rush4, sta+t4.4 never reach the pupal stage. Expression of rushScer\UAS.T:Avic\GFP under the control of Scer\GAL4arm.PS rescues this early lethality.