C2741607T
Q156term | Hrs-PA; Q269term | Hrs-PB; Q269term | Hrs-PC
Q269term
Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.
Amino acid replacement: Q270term.
Loss of photoreceptors is seen in HrsD28/Df(2L)Exel6277 mutant eye discs.
Homozygous mushroom body gamma neuron clones show normal axon pruning.
HrsD28 follicular epithelium clones display normal epithelial morphology.
There is no detectable phenotype in HrsD28 mosaic eye imaginal discs or imaginal discs consisting predominantly of mutant cells.
Eye discs consisting predominantly of cells mutant for HrsD28 progress to form adult eyes. These are smaller than wild-type and have a rough appearance but contain some mutant photoreceptors. The scarcity of mutant adult photoreceptors might be due to cell death, as apoptotic cells are seen these mutants.
Homozygotes die at the early pupal stage.
HrsD28 mosaic eye disc clones have a similar number of acidified endosomes to wild type.
Oocytes mutant for HrsD28 exhibit yolk granules containing intralumenal vesicles and isolated multivesicular bodies, although they are less abundant in the wild-type. In addition, internalised yolk proteins are detected in regularly shaped yolk granules.
HrsD28/Df(2L)Exel6277 eye discs show a loss of photoreceptor cells.
HrsD28 mutant cells are preferentially located at the front of the cluster during border cell posterior and dorsal migration in mosaic border cell clusters consisting of both wild-type and mutant cells.
HrsD28 clones within the wing discs contain enlarged late endosomes.
Only slight defects in border cell migration are apparent when border cells are part of HrsD28 homozygous somatic clones.
In mutant larval garland cells endosomes are dramatically enlarged. Wild-type endosomes mature by forming invaginations of their limiting membrane and eventually collapse upon themselves. In mutant garland cells there is a strong reduction the relative number of invaginated (5 fold) and collapsed (10 fold) endosomes. There is also a 5 fold reduction of small multivesicular bodies (MVBs) compared to wild-type.
Hemizygotes die as larvae.
HrsD28 has increased cell death | somatic clone | third instar larval stage phenotype, enhanceable by Rbf15aΔ/Rbf15aΔ
HrsD28 has lethal | pupal stage phenotype, enhanceable by sta+t4.4/Df(1)rush4
Df(1)rush4, HrsD28, sta+t4.4 has lethal phenotype, suppressible | partially by rushUAS.GFP/Scer\GAL4arm.PS
HrsD28 has lethal | pupal stage phenotype, suppressible by rushUAS.GFP/Scer\GAL4arm.PS
HrsD28/HrsD28 is an enhancer of increased cell death | somatic clone | third instar larval stage phenotype of Rbf15aΔ
Hrs[+]/HrsD28 is a suppressor of abnormal neurophysiology | third instar larval stage phenotype of sky1/sky2
Hrs[+]/HrsD28 is a suppressor of abnormal neuroanatomy phenotype of spin10403
HrsD28, Rab81/Rab8[+] has abnormal neuroanatomy phenotype
HrsD28, Stam2L2896 has abnormal neuroanatomy | somatic clone phenotype
Df(2L)Exel6277/HrsD28, Snx16Δ1/Snx16e01600 has abnormal neuroanatomy | larval stage phenotype
HrsD28 has eye disc | somatic clone | third instar larval stage phenotype, enhanceable by Rbf15aΔ/Rbf15aΔ
HrsD28 has wing disc | somatic clone | third instar larval stage phenotype, enhanceable by Rbf15aΔ/Rbf15aΔ
HrsD28/HrsD28 is an enhancer of eye disc | somatic clone | third instar larval stage phenotype of Rbf15aΔ
HrsD28/HrsD28 is an enhancer of wing disc | somatic clone | third instar larval stage phenotype of Rbf15aΔ
Hrs[+]/HrsD28 is a suppressor of embryonic/larval neuromuscular junction phenotype of sky1/sky2
Hrs[+]/HrsD28 is a suppressor of glial cell phenotype of spin10403
HrsD28 is a suppressor of external sensory organ phenotype of l(2)gd124
HrsD28 is a non-suppressor of eye disc | somatic clone phenotype of shi7C7
HrsD28 is a non-suppressor of photoreceptor | increased number | somatic clone | third instar larval stage phenotype of shi7C7
HrsD28, Rab81/Rab8[+] has embryonic/larval neuromuscular junction | third instar larval stage phenotype
HrsD28, Rab81/Rab8[+] has NMJ bouton | third instar larval stage phenotype
HrsD28, Stam2L2896 has gamma Kenyon cell | somatic clone phenotype
HrsD28, Stam2L2896 has eye | somatic clone phenotype
HrsD28, Stam2L2896 has imaginal disc epithelial cell | somatic clone phenotype
Df(2L)Exel6277/HrsD28, Snx16Δ1/Snx16e01600 has NMJ bouton | increased number | larval stage phenotype
Somatic clones of Rbf15aΔ;HrsD28 double homozygous cells display significantly higher levels of apoptosis in third instar larval wing and eye discs, even in clones posterior to the eye disc morphogenetic furrow, compared to clones homozygous for either Rbf15aΔ or HrsD28 alone.
Differentiation of the R8 photoreceptor in HrsD28/HrsD28 somatic clones in the third instar larval eye disc is normal but differentiation of other photoreceptors is significantly delayed.
HrsD28/HrsD28 Stam2L2896/Stam2L2896 MARCM ddaC double mutant clones show dendrite pruning similar to wild type at 18hr APF.
HrsD28 Stam2L2896 double homozygous mushroom body gamma neuron clones show defects in axon pruning.
HrsD28 Stam2L2896 follicular epithelium clones display normal epithelial morphology.
HrsD28 Stam2L2896 l(2)gl4 follicular epithelium clones display normal epithelial morphology.
HrsD28 Stam2L2896 double mutant imaginal eye disc clonal cells form large clones of mesenchymal-like cells. Mosaic eye imaginal discs or eye imaginal discs consistently predominantly of cells mutant for both HrsD28 and Stam2L2896 show a loss of epithelial architecture phenotype.
Eye discs consisting predominantly of cells mutant for HrsD28 Stam2L2896 progress to form adult eyes. These are smaller than wild-type and have a rough appearance but contain some mutant photoreceptors. The scarcity of mutant adult photoreceptors might be due to cell death, as apoptotic cells are seen these mutants.
The ectopic N activation phenotype (ectopic expression of Ecol\lacZDdc.be2.E(spl)m8-HLH) in l(2)gd1d7 wing disk clones is suppressed if cells are also mutant for HrsD28.
While HrsD28 homozygotes die at the early pupal stage, animals homozygous for HrsD28 and Df(1)rush4, sta+t4.4 never reach the pupal stage. Expression of rushScer\UAS.T:Avic\GFP under the control of Scer\GAL4arm.PS rescues this early lethality.
Average bouton number and average satellite bouton number at the neuromuscular junction is significantly increased compared to wild type in Snx16e01600 HrsD28/Snx16Δ1 Df(2L)Exel6277 double mutants.