FlyBase Allele Report: Dmel\Hrs[D28]

General Information

Symbol

Dmel\Hrs^D28

Species

D. melanogaster

Name

FlyBase ID

FBal0039519

Feature type

allele

Associated gene

Dmel\Hrs

Associated Insertion(s)

Carried in Construct

Also Known As

l(2)23AB5^D28

Key Links

Genomic Maps

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Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Littleton and Bellen, 1994)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

point_mutation

2L:2,741,607..2,741,607 [+]

Nucleotide change:

C2741607T

Amino acid change:

Q156term | Hrs-PA; Q269term | Hrs-PB; Q269term | Hrs-PC

Reported amino acid change:

Q269term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Lloyd et al., 2002)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Cytology

Nature of the lesion

Statement

Reference

Amino acid replacement: Q270term.

(Lloyd et al., 2002)

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

border follicle cell | somatic clone

(Jekely et al., 2005)

embryonic/larval garland cell

(Lloyd et al., 2002)

endosome

(Lloyd et al., 2002)

eye | somatic clone

(Tognon et al., 2014)

eye-antennal disc | somatic clone

(Lu and Bilder, 2005)

eye disc | somatic clone

(Legent et al., 2015)

eye disc | somatic clone | third instar larval stage

(Sheng et al., 2016)

eye photoreceptor cell, with Df(2L)Exel6277

(Miura et al., 2008)

imaginal disc epithelial cell | somatic clone

(Tognon et al., 2014)

late endosome | somatic clone

(Rives et al., 2006)

oocyte

(Vaccari et al., 2009)

photoreceptor | somatic clone | third instar larval stage

(Legent et al., 2015)

photoreceptor neuron | somatic clone

(Tognon et al., 2014)

photoreceptor neuron | somatic clone | third instar larval stage

(Sheng et al., 2016)

vesicle

(Vaccari et al., 2009)

wing disc | somatic clone | third instar larval stage

(Sheng et al., 2016)

Detailed Description

Statement

Reference

Somatic clones of Hrs^D28/Hrs^D28 cells in the third instar larval eye disc display low levels of apoptosis (detected by Caspase-3 staining).

(Sheng et al., 2016)

Loss of photoreceptors is seen in Hrs^D28/Df(2L)Exel6277 mutant eye discs.

(Legent et al., 2015)

Hrs^D28/Hrs^D28 MARCM ddaC clones show dendrite pruning similar to wild type at 18hr APF.

(Loncle et al., 2015)

Homozygous mushroom body gamma neuron clones show normal axon pruning.

(Issman-Zecharya and Schuldiner, 2014)

Hrs^D28 follicular epithelium clones display normal epithelial morphology.

There is no detectable phenotype in Hrs^D28 mosaic eye imaginal discs or imaginal discs consisting predominantly of mutant cells.

Eye discs consisting predominantly of cells mutant for Hrs^D28 progress to form adult eyes. These are smaller than wild-type and have a rough appearance but contain some mutant photoreceptors. The scarcity of mutant adult photoreceptors might be due to cell death, as apoptotic cells are seen these mutants.

(Tognon et al., 2014)

Homozygotes die at the early pupal stage.

(Gailite et al., 2012)

Hrs^D28 mosaic eye disc clones have a similar number of acidified endosomes to wild type.

(Vaccari et al., 2010)

Oocytes mutant for Hrs^D28 exhibit yolk granules containing intralumenal vesicles and isolated multivesicular bodies, although they are less abundant in the wild-type. In addition, internalised yolk proteins are detected in regularly shaped yolk granules.

(Vaccari et al., 2009)

Hrs^D28/Df(2L)Exel6277 eye discs show a loss of photoreceptor cells.

(Miura et al., 2008)

Hrs^D28 mutant cells are preferentially located at the front of the cluster during border cell posterior and dorsal migration in mosaic border cell clusters consisting of both wild-type and mutant cells.

(Bianco et al., 2007)

Hrs^D28 clones within the wing discs contain enlarged late endosomes.

(Rives et al., 2006)

Only slight defects in border cell migration are apparent when border cells are part of Hrs^D28 homozygous somatic clones.

(Jekely et al., 2005)

In mutant larval garland cells endosomes are dramatically enlarged. Wild-type endosomes mature by forming invaginations of their limiting membrane and eventually collapse upon themselves. In mutant garland cells there is a strong reduction the relative number of invaginated (5 fold) and collapsed (10 fold) endosomes. There is also a 5 fold reduction of small multivesicular bodies (MVBs) compared to wild-type.

(Lloyd et al., 2002)

Hemizygotes die as larvae.

(Littleton and Bellen, 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Hrs^D28 has increased cell death | somatic clone | third instar larval stage phenotype, enhanceable by Rbf^15aΔ/Rbf^15aΔ

(Sheng et al., 2016)

Hrs^D28 has lethal | pupal stage phenotype, enhanceable by sta^+t4.4/Df(1)rush⁴

(Gailite et al., 2012)

Suppressed by

Statement

Reference

Df(1)rush⁴, Hrs^D28, sta^+t4.4 has lethal phenotype, suppressible | partially by rush^UAS.GFP/Scer\GAL4^arm.PS

(Gailite et al., 2012)

Hrs^D28 has lethal | pupal stage phenotype, suppressible by rush^UAS.GFP/Scer\GAL4^arm.PS

(Gailite et al., 2012)

Enhancer of

Statement

Reference

Hrs^D28/Hrs^D28 is an enhancer of increased cell death | somatic clone | third instar larval stage phenotype of Rbf^15aΔ

(Sheng et al., 2016)

Suppressor of

Statement

Reference

Hrs[+]/Hrs^D28 is a suppressor of abnormal neurophysiology | third instar larval stage phenotype of sky¹/sky²

(Uytterhoeven et al., 2011)

Hrs[+]/Hrs^D28 is a suppressor of abnormal neuroanatomy phenotype of spin¹⁰⁴⁰³

(Yuva-Aydemir et al., 2011)

Hrs^D28 is a suppressor of visible phenotype of l(2)gd1²⁴

(Gallagher and Knoblich, 2006)

Other

Statement

Reference

Hrs^D28, Rab8¹/Rab8[+] has abnormal neuroanatomy phenotype

(West et al., 2015)

Hrs^D28, Stam^2L2896 has abnormal neuroanatomy | somatic clone phenotype

(Issman-Zecharya and Schuldiner, 2014)

Df(2L)Exel6277/Hrs^D28, Snx16^Δ1/Snx16^e01600 has abnormal neuroanatomy | larval stage phenotype

(Rodal et al., 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

Hrs^D28 has eye disc | somatic clone | third instar larval stage phenotype, enhanceable by Rbf^15aΔ/Rbf^15aΔ

(Sheng et al., 2016)

Hrs^D28 has wing disc | somatic clone | third instar larval stage phenotype, enhanceable by Rbf^15aΔ/Rbf^15aΔ

(Sheng et al., 2016)

Enhancer of

Statement

Reference

Hrs^D28/Hrs^D28 is an enhancer of eye disc | somatic clone | third instar larval stage phenotype of Rbf^15aΔ

(Sheng et al., 2016)

Hrs^D28/Hrs^D28 is an enhancer of wing disc | somatic clone | third instar larval stage phenotype of Rbf^15aΔ

(Sheng et al., 2016)

Suppressor of

Statement

Reference

Hrs^D28 is a suppressor of phenotype of l(2)gd1^d7

(Schneider et al., 2013)

Hrs[+]/Hrs^D28 is a suppressor of embryonic/larval neuromuscular junction phenotype of sky¹/sky²

(Uytterhoeven et al., 2011)

Hrs[+]/Hrs^D28 is a suppressor of eye disc phenotype of spin¹⁰⁴⁰³

(Yuva-Aydemir et al., 2011)

Hrs[+]/Hrs^D28 is a suppressor of glial cell phenotype of spin¹⁰⁴⁰³

(Yuva-Aydemir et al., 2011)

Hrs^D28 is a suppressor of external sensory organ phenotype of l(2)gd1²⁴

(Gallagher and Knoblich, 2006)

NOT Suppressor of

Statement

Reference

Hrs^D28 is a non-suppressor of eye disc | somatic clone phenotype of shi^7C7

(Legent et al., 2015)

Hrs^D28 is a non-suppressor of photoreceptor | increased number | somatic clone | third instar larval stage phenotype of shi^7C7

(Legent et al., 2015)

Other

Statement

Reference

Hrs^D28, Rab8¹/Rab8[+] has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(West et al., 2015)

Hrs^D28, Rab8¹/Rab8[+] has NMJ bouton | third instar larval stage phenotype

(West et al., 2015)

Hrs^D28, Stam^2L2896 has gamma Kenyon cell | somatic clone phenotype

(Issman-Zecharya and Schuldiner, 2014)

Hrs^D28, Stam^2L2896 has eye | somatic clone phenotype

(Tognon et al., 2014)

Hrs^D28, Stam^2L2896 has imaginal disc epithelial cell | somatic clone phenotype

(Tognon et al., 2014)

Df(2L)Exel6277/Hrs^D28, Snx16^Δ1/Snx16^e01600 has NMJ bouton | increased number | larval stage phenotype

(Rodal et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Somatic clones of Rbf^15aΔ;Hrs^D28 double homozygous cells display significantly higher levels of apoptosis in third instar larval wing and eye discs, even in clones posterior to the eye disc morphogenetic furrow, compared to clones homozygous for either Rbf^15aΔ or Hrs^D28 alone.

Differentiation of the R8 photoreceptor in Hrs^D28/Hrs^D28 somatic clones in the third instar larval eye disc is normal but differentiation of other photoreceptors is significantly delayed.

(Sheng et al., 2016)

Hrs^D28 does not suppress the increased photoreceptor differentiation seen in shi^7C7 mutant eye disc clones.

(Legent et al., 2015)

Hrs^D28/Hrs^D28 Stam^2L2896/Stam^2L2896 MARCM ddaC double mutant clones show dendrite pruning similar to wild type at 18hr APF.

(Loncle et al., 2015)

Hrs^D28 Stam^2L2896 double homozygous mushroom body gamma neuron clones show defects in axon pruning.

(Issman-Zecharya and Schuldiner, 2014)

Hrs^D28 Stam^2L2896 follicular epithelium clones display normal epithelial morphology.

Hrs^D28 Stam^2L2896 l(2)gl⁴ follicular epithelium clones display normal epithelial morphology.

Hrs^D28 Stam^2L2896 double mutant imaginal eye disc clonal cells form large clones of mesenchymal-like cells. Mosaic eye imaginal discs or eye imaginal discs consistently predominantly of cells mutant for both Hrs^D28 and Stam^2L2896 show a loss of epithelial architecture phenotype.

Eye discs consisting predominantly of cells mutant for Hrs^D28 Stam^2L2896 progress to form adult eyes. These are smaller than wild-type and have a rough appearance but contain some mutant photoreceptors. The scarcity of mutant adult photoreceptors might be due to cell death, as apoptotic cells are seen these mutants.

(Tognon et al., 2014)

The ectopic N activation phenotype (ectopic expression of Ecol\lacZ^{Ddc.be2.E(spl)m8-HLH}) in l(2)gd1^d7 wing disk clones is suppressed if cells are also mutant for Hrs^D28.

(Schneider et al., 2013)

While Hrs^D28 homozygotes die at the early pupal stage, animals homozygous for Hrs^D28 and Df(1)rush⁴, sta^+t4.4 never reach the pupal stage. Expression of rush^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^arm.PS rescues this early lethality.

(Gailite et al., 2012)

Average bouton number and average satellite bouton number at the neuromuscular junction is significantly increased compared to wild type in Snx16^e01600 Hrs^D28/Snx16^Δ1 Df(2L)Exel6277 double mutants.

(Rodal et al., 2011)

The increased excitatory junctional current (EJC) amplitude seen at the neuromuscular junction in sky¹/sky² third instar larvae is suppressed if the larvae are also heterozygous for Hrs^D28.

(Uytterhoeven et al., 2011)

Hrs^D28/+ suppresses the glial migration defects seen in spin¹⁰⁴⁰³ eye discs.

(Yuva-Aydemir et al., 2011)

The ectopic upregulation of N signaling seen in l(2)gd1^d7 clones is suppressed when clones also carry the Hrs^D28 mutation.

(Childress et al., 2006)

The external sensory organ multi-socket phenotype in l(2)gd1²⁴ animals is suppressed in a Hrs^D28 mutant background

(Gallagher and Knoblich, 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Hrs^D28 is rescued by Hrs^+t11.5

(Lloyd et al., 2002)

Hrs^D28 is rescued by Hrs^hs.PB

(Lloyd et al., 2002)

Comments

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

42691

Hrs^D28; P{UAS-arr.flu}3/T(2;3)B3, CyO: TM6B, Tb⁺

56816

w^*; Hrs^D28 Stam^2L2896 P{neoFRT}40A/CyO, P{GAL4-twi.G}2.2, P{UAS-2xEGFP}AH2.2

54574

P{hsFLP}12, y¹ w^*; Hrs^D28 P{neoFRT}40A/In(2LR)Gla, wg^Gla-1 PPO1^Bc

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (9)

Reported As

Symbol Synonym

HRS^D28

(West et al., 2015)

Hrs^D28

(Wang et al., 2022, Pannen et al., 2020, Kahsai and Cook, 2018, Gomez-Lamarca et al., 2015, Legent et al., 2015, Loncle et al., 2015, Issman-Zecharya and Schuldiner, 2014, Tognon et al., 2014, Gailite et al., 2012, Du et al., 2011, Richardson and Pichaud, 2010, Vaccari et al., 2010, Yan et al., 2009, Miura et al., 2008, Vaccari et al., 2008, Bianco et al., 2007, Hennig et al., 2006)

hrs_D28

(Yuva-Aydemir et al., 2011)

hrs^28D

(Rusten et al., 2006)

hrs^d28

(Gallagher and Knoblich, 2006)

hrsD28

(Jaekel and Klein, 2006)

l(2)23AB5^D28

(Lloyd et al., 2002, Littleton and Bellen, 1994)

vps27^D28

(Ahmad et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (48)

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