FB2025_01 , released February 20, 2025
Allele: Ecol\lacZftz.PC
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General Information
Symbol
Ecol\lacZftz.PC
Species
E. coli
Name
FlyBase ID
FBal0041452
Feature type
allele
Associated gene
Ecol\lacZ
Associated Insertion(s)
Carried in Construct
P{ftz-lacC}
Also Known As
ftz-lacZ
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Yu and Pick, 1995, Callahan and Thomas, 1994, Doe, 1992, Kellum and Schedl, 1992, Parkhurst and Ish-Horowicz, 1991, Parkhurst and Ish-Horowicz, 1991, Kellerman et al., 1990, Nambu et al., 1990, Zak et al., 1990, Ambrosio et al., 1989, Lawrence and Johnston, 1989, Tremml and Bienz, 1989, Thomas et al., 1988, Hiromi and Gehring, 1987, Ish-Horowicz and Pinchin, 1987, Lawrence et al., 1987, Hiromi et al., 1985)
Progenitor genotype
Carried in construct
P{ftz-lacC}
Cytology
Description

Approximately 6kb of ftz 5' flanking sequence, including the zebra, neurogenic and upstream elements drives lacZ expression.

(Yu and Pick, 1995)

A full length ftz promoter fragment which extends from -6.1kb (relative to the translation start site) to the second codon is fused in frame to lacZ.

(Hiromi et al., 1985)
Allele components
Component
Use(s)
Regulatory region(s)
ftz_lacC
Encoded product / tool
lacZ
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      No Assay Recorded
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      embryonic stage 13 -- 14
      parasegment 4
      (Tremml and Bienz, 1989)
      parasegment 8
      (Tremml and Bienz, 1989)
      parasegment 12
      (Tremml and Bienz, 1989)
      distribution deduced from reporter
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      embryonic stage 10 -- 11
      mesoderm | pair rule expression pattern
      (Hiromi and Gehring, 1987)
      ectoderm | pair rule expression pattern
      (Hiromi and Gehring, 1987)
      embryonic stage 13 -- 14
      parasegment 10
      (Tremml and Bienz, 1989)
      parasegment 2
      (Tremml and Bienz, 1989)
      parasegment 6
      (Tremml and Bienz, 1989)
      embryonic stage 15 -- 16
      presumptive embryonic/larval central nervous system
      (Hiromi and Gehring, 1987)
      radioisotope in situ
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      embryonic stage 4 -- 17
      organism | pair rule expression pattern
      (Hiromi et al., 1985)
      embryonic stage 6 -- 17
      organism | pair rule expression pattern
      (Hiromi et al., 1985)
      embryonic stage 11 -- 17
      neuroblast | restricted
      (Hiromi et al., 1985)
      embryonic stage 13 -- 17
      larval ventral nerve cord | segmentally repeated
      (Hiromi et al., 1985)
      epidermis | pair rule expression pattern
      (Hiromi et al., 1985)
      western blot
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      embryonic stage
      (Hiromi et al., 1985)
      first instar larval stage
      (Hiromi et al., 1985)
      Additional Information
      Statement
      Reference

      ftz is expressed in alternating parasegments in the germ band retracted embryo between parasgements 2 and 12.

      (Tremml and Bienz, 1989)

      In germ-band extended embryos, strong expression in the mesoderm and ectoderm, in even parasegments. In later embryos, expression is observed in the CNS. Expression pattern characteristic of that observed for ftz.

      (Hiromi and Gehring, 1987)

      In embryos, peak accumulation is from 4.5-10.5 hours after egg-laying.

      (Hiromi et al., 1985)

      In gastrulating embryos, the first stripe is posterior to the cephalic furrow. Seven stripes observed; most posterior stripe thicker and occasionally resolves as two stripes. During mid-embryogenesis expression also observed in pairs of large cells; assumed to be neuroblasts. The most conspicuous of these pairs flank the ventral midline, one pair per segment. Observed stripes in epidermis correspond to parasegments, not segments. Expression throughout the ventral nerve cord (not in pair-rule pattern).

      (Hiromi et al., 1985)
       
      Marker for
      Reflects expression of
      ftz
      (Franke and Baker, 1999, Doe, 1992, Tremml and Bienz, 1989, Hiromi and Gehring, 1987, Hiromi et al., 1985)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (61)
      Notes on Origin
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Ecol\lacZftz.PC
      lacZftz.PC
      Name Synonyms
      Secondary FlyBase IDs
        References (56)