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General Information
Symbol
Dmel\edslH8
Species
D. melanogaster
Name
FlyBase ID
FBal0043015
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Mutagen
    Nature of the Allele
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    Nucleotide change:

    T4098950A

    Amino acid change:

    C618S | ed-PA; C618S | ed-PB; C618S | ed-PC

    Reported amino acid change:

    C618S

    Comment:

    Amino acid replacement reported as reported as Cys to Ser, which could result from a nucleotide substitution in the first or second base of the codon. The mutation was annotated at the first base of the codon.

    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference

    Amino acid replacement: C618S.

    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease-implicated variant(s)
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    Many edslH8/edK1102 ommatidia over- or under-rotate; the variance (s.d.) in degree of rotation is greater than in wild type in adult eyes. The mutant s.d. does not become statistically distinct from wild type until row 7 in the third larval instar eye disc, approximately 2-3 rows after the initiation of rotation (the time at which the anterior and posterior cone cells join the ommatidial precursor). The s.d. is still significantly greater in the mutants than in wild type at row 15, when rotation is complete in wild type.

    edslH8 animals predominantly die as embryos although some develop into second instar

    larvae. In these larvae, defects in ventrolateral muscles and lateral transverse muscles are evident with both muscle groups forming aberrant projections and ectopic adhesion points. Consistent and prominent defects in muscle morphology can also be observed at the embryonic stage. The same defects are present in ed1X5/edslH8 transheterozygotes.

    The number of macrochaetae/heminotum in ed1X5/edslH8 mutants is - anterior+posterior notopleural: 2.10, presutural: 1.28, anterior supraalar: 1.13, posterior supraalar: 1.05, anterior postalar: 1.80, posterior postalar: 1.85, anterior+posterior dorsocentral: 2.80 and anterior+posterior scutellar: 2.63. The density of microchaetae on the notum is also increased in these flies. The number of macrochaetae/heminotum in edslH8/Df(2L)ed-dp mutants is - anterior+posterior notopleural: 1.80, presutural: 1.27, anterior supraalar: 1.06, posterior supraalar: 1.01, anterior postalar: 1.87, posterior postalar: 2.11, anterior+posterior dorsocentral: 3.00 and anterior+posterior scutellar: 2.24. The number of macrochaetae/heminotum in edslH8/edlF20 mutants is - anterior+posterior notopleural: 2.04, presutural: 1.19, anterior supraalar: 1.07, posterior supraalar: 1.06, anterior postalar: 1.87, posterior postalar: 2.09, anterior+posterior dorsocentral: 2.54 and anterior+posterior scutellar: 3.24.

    Surviving adults have rough eyes and extra wing veins. 33% of ommatidia of ed1X5/edslH8 flies have extra R7-like cells. 26% of ommatidia have extra outer photoreceptors and 6% have fewer than normal outer photoreceptors. Mystery cells are transformed into neuronal photoreceptor cells. 69% of ommatidia have 5-6 instead of 4 cone cells, and 10% have three primary pigment cells. ed1X5/edslH8 flies show an enlarged wing with extra wing vein material. Females of the genotype edslH8/Df(2L)ed-dp are fertile and the resulting eggs are normal in appearance.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Enhanced by
    Statement
    Reference

    edslH8/ed1X5 has visible phenotype, enhanceable by DlRevF10/Dl[+]

    Suppressed by
    Statement
    Reference

    edslH8/ed1X5 has visible phenotype, suppressible | partially by H2/H[+]

    NOT Suppressor of
    Statement
    Reference

    edslH8/ed1X5 is a non-suppressor of visible | dominant phenotype of NMcd1

    edslH8/ed1X5 is a non-suppressor of visible | dominant phenotype of NAx-M1

    Other
    Statement
    Reference
    Phenotype Manifest In
    Enhanced by
    Statement
    Reference

    edK1102/edslH8 has ommatidium phenotype, enhanceable by fred[+]/fredH24

    edslH8/ed1X5 has macrochaeta | ectopic phenotype, enhanceable by DlRevF10/Dl[+]

    Suppressed by
    Statement
    Reference
    NOT suppressed by
    Statement
    Reference

    edslH8/ed1X5 has microchaeta phenotype, non-suppressible by H2/H[+]

    Suppressor of
    Statement
    Reference

    edslH8/ed1X5 is a suppressor of ommatidium phenotype of aopACT.sev

    edslH8/ed1X5 is a suppressor of ommatidium phenotype of sina3/sina2

    edslH8/ed1X5 is a suppressor of ommatidium phenotype of pnt1277/pntΔ88

    NOT Suppressor of
    Statement
    Reference

    edslH8/ed1X5 is a non-suppressor of microchaeta phenotype of NMcd1

    edslH8/ed1X5 is a non-suppressor of macrochaeta phenotype of NAx-M1

    Other
    Additional Comments
    Genetic Interactions
    Statement
    Reference

    fredH24 dominantly enhances the phenotype of under- or over-rotation of ommatidia that is seen in edK1102/edslH8 animals.

    Expression of GripΔ1-3.Scer\UAS, under the control of Scer\GAL4twi.PG in a edslH8 background causes a mild lateral transverse muscle phenotype similar to that seen in edslH8 embryos without transgene expression. This is in contrast to the more severe phenotype seen when GripΔ1-3.Scer\UAS is expressed in a wild-type background.

    Expression of EgfrDN.Scer\UAS.cBa under the control of Scer\GAL4ap-md544 partially suppresses the extra bristle phenotype seen in ed1X5/edslH8 flies. The number of macrochaetae/heminotum in animals expressing EgfrDN.Scer\UAS.cBa under the control of Scer\GAL4ap-md544 in a ed1X5/edslH8 background is - anterior+posterior notopleural: 2.15, presutural: 1.05, anterior supraalar: 0.80, posterior supraalar: 0, anterior postalar: 0.60, posterior postalar: 0, anterior+posterior dorsocentral: 1.30 and anterior+posterior scutellar: 2.25. N55e11/+ ; ed1X5/edslH8 adults show an almost complete absence of microchaetae on the notum. DlRevF10/+ increases the number of extra macrochaetae on the notum seen in ed1X5/edslH8 adults. H2/+ almost completely eliminates the extra macrochaetae seen on the notum of ed1X5/edslH8 animals, although the increased density of microchaetae seen in ed1X5/edslH8 flies is not reduced by H2/+. The low density of microchaetae on the notum seen in NMcd1/+ flies is not rescued by ed1X5/edslH8. The loss of macrochaetae on the notum seen in NAx-M1/+ flies is not rescued by ed1X5/edslH8.

    Xenogenetic Interactions
    Statement
    Reference
    Complementation and Rescue Data
    Rescued by
    Comments
    Images (0)
    Mutant
    Wild-type
    Stocks (0)
    Notes on Origin
    Discoverer
    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (3)
    Reported As
    Name Synonyms
    Secondary FlyBase IDs
      References (6)