FlyBase Allele Report: Dmel\ed[slH8]

General Information

Symbol

Dmel\ed^slH8

Species

D. melanogaster

Name

FlyBase ID

FBal0043015

Feature type

allele

Associated gene

Dmel\ed

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

loss of function allele

Mutagen

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Swan et al., 2006, Escudero et al., 2003)

loss of function allele

(Bai et al., 2001)

Mutagen

Progenitor genotype

Cytology

Description

Amino acid replacement: C618S.

(Escudero et al., 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:4,098,950..4,098,950 [+]

Nucleotide change:

T4098950A

Amino acid change:

C618S | ed-PA; C618S | ed-PB; C618S | ed-PC

Reported amino acid change:

C618S

Comment:

Amino acid replacement reported as reported as Cys to Ser, which could result from a nucleotide substitution in the first or second base of the codon. The mutation was annotated at the first base of the codon.

(Escudero et al., 2003)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

partially lethal - majority die

(de Belle et al., 1993)

partially lethal - majority die | recessive

(Bai et al., 2001)

viable (with ed^1X5)

(Escudero et al., 2003)

viable (with ed^lF20)

(Escudero et al., 2003)

visible (with Df(2L)ed-dp)

(Escudero et al., 2003)

visible (with ed^1X5)

(Escudero et al., 2003, Bai et al., 2001)

visible (with ed^lF20)

(Escudero et al., 2003)

visible | recessive

(Bai et al., 2001)

Phenotype Manifest In

cone cell (with ed^1X5)

(Bai et al., 2001)

eye

(Bai et al., 2001)

lateral transverse muscle cell

(Swan et al., 2006)

lateral transverse muscle cell (with ed^1X5)

(Swan et al., 2006)

macrochaeta | ectopic (with Df(2L)ed-dp)

(Escudero et al., 2003)

macrochaeta | ectopic (with ed^1X5)

(Escudero et al., 2003)

macrochaeta | ectopic (with ed^lF20)

(Escudero et al., 2003)

microchaeta | ectopic (with ed^1X5)

(Escudero et al., 2003)

mystery cell (with ed^1X5)

(Bai et al., 2001)

ommatidial precursor cluster (with ed^K1102)

(Fetting et al., 2009)

ommatidium (with ed^1X5)

(Bai et al., 2001)

ommatidium (with ed^K1102)

(Fetting et al., 2009)

photoreceptor cell R1 (with ed^1X5)

(Bai et al., 2001)

photoreceptor cell R1 | increased number (with ed^1X5)

(Bai et al., 2001)

photoreceptor cell R2 (with ed^1X5)

(Bai et al., 2001)

photoreceptor cell R2 | increased number (with ed^1X5)

(Bai et al., 2001)

photoreceptor cell R3 (with ed^1X5)

(Bai et al., 2001)

photoreceptor cell R3 | increased number (with ed^1X5)

(Bai et al., 2001)

photoreceptor cell R4 (with ed^1X5)

(Bai et al., 2001)

photoreceptor cell R4 | increased number (with ed^1X5)

(Bai et al., 2001)

photoreceptor cell R5 (with ed^1X5)

(Bai et al., 2001)

photoreceptor cell R5 | increased number (with ed^1X5)

(Bai et al., 2001)

photoreceptor cell R6 (with ed^1X5)

(Bai et al., 2001)

photoreceptor cell R6 | increased number (with ed^1X5)

(Bai et al., 2001)

photoreceptor cell R7 | increased number (with ed^1X5)

(Bai et al., 2001)

ventral longitudinal muscle cell

(Swan et al., 2006)

ventral longitudinal muscle cell (with ed^1X5)

(Swan et al., 2006)

wing (with ed^1X5)

(Bai et al., 2001)

wing vein | increased number

(Bai et al., 2001)

wing vein | increased number (with ed^1X5)

(Bai et al., 2001)

Detailed Description

Statement

Reference

Many ed^slH8/ed^K1102 ommatidia over- or under-rotate; the variance (s.d.) in degree of rotation is greater than in wild type in adult eyes. The mutant s.d. does not become statistically distinct from wild type until row 7 in the third larval instar eye disc, approximately 2-3 rows after the initiation of rotation (the time at which the anterior and posterior cone cells join the ommatidial precursor). The s.d. is still significantly greater in the mutants than in wild type at row 15, when rotation is complete in wild type.

(Fetting et al., 2009)

ed^slH8 animals predominantly die as embryos although some develop into second instar

larvae. In these larvae, defects in ventrolateral muscles and lateral transverse muscles are evident with both muscle groups forming aberrant projections and ectopic adhesion points. Consistent and prominent defects in muscle morphology can also be observed at the embryonic stage. The same defects are present in ed^1X5/ed^slH8 transheterozygotes.

(Swan et al., 2006)

The number of macrochaetae/heminotum in ed^1X5/ed^slH8 mutants is - anterior+posterior notopleural: 2.10, presutural: 1.28, anterior supraalar: 1.13, posterior supraalar: 1.05, anterior postalar: 1.80, posterior postalar: 1.85, anterior+posterior dorsocentral: 2.80 and anterior+posterior scutellar: 2.63. The density of microchaetae on the notum is also increased in these flies. The number of macrochaetae/heminotum in ed^slH8/Df(2L)ed-dp mutants is - anterior+posterior notopleural: 1.80, presutural: 1.27, anterior supraalar: 1.06, posterior supraalar: 1.01, anterior postalar: 1.87, posterior postalar: 2.11, anterior+posterior dorsocentral: 3.00 and anterior+posterior scutellar: 2.24. The number of macrochaetae/heminotum in ed^slH8/ed^lF20 mutants is - anterior+posterior notopleural: 2.04, presutural: 1.19, anterior supraalar: 1.07, posterior supraalar: 1.06, anterior postalar: 1.87, posterior postalar: 2.09, anterior+posterior dorsocentral: 2.54 and anterior+posterior scutellar: 3.24.

(Escudero et al., 2003)

Surviving adults have rough eyes and extra wing veins. 33% of ommatidia of ed^1X5/ed^slH8 flies have extra R7-like cells. 26% of ommatidia have extra outer photoreceptors and 6% have fewer than normal outer photoreceptors. Mystery cells are transformed into neuronal photoreceptor cells. 69% of ommatidia have 5-6 instead of 4 cone cells, and 10% have three primary pigment cells. ed^1X5/ed^slH8 flies show an enlarged wing with extra wing vein material. Females of the genotype ed^slH8/Df(2L)ed-dp are fertile and the resulting eggs are normal in appearance.

(Bai et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

ed^slH8/ed^1X5 has visible phenotype, enhanceable by Delta^RevF10/Dl[+]

(Escudero et al., 2003)

Suppressed by

Statement

Reference

ed^slH8/ed^1X5 has visible phenotype, suppressible | partially by Scer\GAL4^ap-md544/Egfr^DN.UAS.cBa

(Escudero et al., 2003)

ed^slH8/ed^1X5 has visible phenotype, suppressible | partially by H²/H[+]

(Escudero et al., 2003)

NOT Suppressor of

Statement

Reference

ed^slH8/ed^1X5 is a non-suppressor of visible | dominant phenotype of N^Ax-M1

(Escudero et al., 2003)

ed^slH8/ed^1X5 is a non-suppressor of visible | dominant phenotype of N^Mcd1

(Escudero et al., 2003)

Other

Statement

Reference

N^55e11, ed^slH8/ed^1X5 has visible phenotype

(Escudero et al., 2003)

N[+]/N^55e11, ed^slH8/ed^1X5 has visible phenotype

(Escudero et al., 2003)

Phenotype Manifest In

Enhanced by

Statement

Reference

ed^K1102/ed^slH8 has ommatidium phenotype, enhanceable by fred[+]/fred^H24

(Fetting et al., 2009)

ed^slH8/ed^1X5 has macrochaeta | ectopic phenotype, enhanceable by Delta^RevF10/Dl[+]

(Escudero et al., 2003)

Suppressed by

Statement

Reference

ed^slH8/ed^1X5 has macrochaeta | ectopic phenotype, suppressible by H²/H[+]

(Escudero et al., 2003)

ed^slH8/ed^1X5 has macrochaeta | ectopic phenotype, suppressible | partially by Scer\GAL4^ap-md544/Egfr^DN.UAS.cBa

(Escudero et al., 2003)

NOT suppressed by

Statement

Reference

ed^slH8/ed^1X5 has microchaeta phenotype, non-suppressible by H²/H[+]

(Escudero et al., 2003)

Suppressor of

Statement

Reference

ed^slH8 is a suppressor of lateral transverse muscle cell phenotype of Grip^Δ1-3.UAS, Scer\GAL4^twi.PG

(Swan et al., 2006)

ed^slH8/ed^1X5 is a suppressor of photoreceptor cell R7 phenotype of pnt¹²⁷⁷/pnt^Δ88

(Bai et al., 2001)

ed^slH8/ed^1X5 is a suppressor of ommatidium phenotype of pnt¹²⁷⁷/pnt^Δ88

(Bai et al., 2001)

ed^slH8/ed^1X5 is a suppressor of photoreceptor cell R7 phenotype of sina³/sina²

(Bai et al., 2001)

ed^slH8/ed^1X5 is a suppressor of ommatidium phenotype of sina³/sina²

(Bai et al., 2001)

ed^slH8/ed^1X5 is a suppressor of photoreceptor cell R7 phenotype of aop^ACT.sev

(Bai et al., 2001)

ed^slH8/ed^1X5 is a suppressor of ommatidium phenotype of aop^ACT.sev

(Bai et al., 2001)

NOT Suppressor of

Statement

Reference

ed^slH8/ed^1X5 is a non-suppressor of macrochaeta phenotype of N^Ax-M1

(Escudero et al., 2003)

ed^slH8/ed^1X5 is a non-suppressor of microchaeta phenotype of N^Mcd1

(Escudero et al., 2003)

ed^slH8/ed^1X5 is a non-suppressor of photoreceptor neuron phenotype of ttk^{88.GMR.Tag:MYC}

(Bai et al., 2001)

Other

Statement

Reference

N^55e11, ed^slH8/ed^1X5 has microchaeta phenotype

(Escudero et al., 2003)

N[+]/N^55e11, ed^slH8/ed^1X5 has microchaeta phenotype

(Escudero et al., 2003)

ed^slH8/ed^1X5, sev¹⁴ has photoreceptor cell R7 | increased number phenotype

(Bai et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

fred^H24 dominantly enhances the phenotype of under- or over-rotation of ommatidia that is seen in ed^K1102/ed^slH8 animals.

(Fetting et al., 2009)

Expression of Grip^{Δ1-3.Scer\UAS}, under the control of Scer\GAL4^twi.PG in a ed^slH8 background causes a mild lateral transverse muscle phenotype similar to that seen in ed^slH8 embryos without transgene expression. This is in contrast to the more severe phenotype seen when Grip^{Δ1-3.Scer\UAS} is expressed in a wild-type background.

(Swan et al., 2006)

Expression of Egfr^{DN.Scer\UAS.cBa} under the control of Scer\GAL4^ap-md544 partially suppresses the extra bristle phenotype seen in ed^1X5/ed^slH8 flies. The number of macrochaetae/heminotum in animals expressing Egfr^{DN.Scer\UAS.cBa} under the control of Scer\GAL4^ap-md544 in a ed^1X5/ed^slH8 background is - anterior+posterior notopleural: 2.15, presutural: 1.05, anterior supraalar: 0.80, posterior supraalar: 0, anterior postalar: 0.60, posterior postalar: 0, anterior+posterior dorsocentral: 1.30 and anterior+posterior scutellar: 2.25. N^55e11/+ ; ed^1X5/ed^slH8 adults show an almost complete absence of microchaetae on the notum. Dl^RevF10/+ increases the number of extra macrochaetae on the notum seen in ed^1X5/ed^slH8 adults. H²/+ almost completely eliminates the extra macrochaetae seen on the notum of ed^1X5/ed^slH8 animals, although the increased density of microchaetae seen in ed^1X5/ed^slH8 flies is not reduced by H²/+. The low density of microchaetae on the notum seen in N^Mcd1/+ flies is not rescued by ed^1X5/ed^slH8. The loss of macrochaetae on the notum seen in N^Ax-M1/+ flies is not rescued by ed^1X5/ed^slH8.

(Escudero et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

ed^slH8 is rescued by ed^hs.PB

(Bai et al., 2001)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

ed^SLH8

(Swan et al., 2006)

ed^SlH8

(Fetting et al., 2009, Swan et al., 2006, Swan et al., 2006)

ed^slH8

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

Open Close